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The map label for this gene is hisS

Identifier: 189025866

GI number: 189025866

Start: 703027

End: 704355

Strand: Reverse

Name: hisS

Synonym: TPASS_0641

Alternate gene names: 189025866

Gene position: 704355-703027 (Counterclockwise)

Preceding gene: 189025869

Following gene: 189025863

Centisome position: 61.81

GC content: 49.89

Gene sequence:

>1329_bases
GTGCGCGTGGGTTCTGCTGTTTCTCCGAAAGTTTTAAAAGGCTTTCGCGATCTTTTACCGGATGAAGAGATTGAGCGTGC
ATTGCTCGTAGAAAAACTGACGGTGGCTTTAAGACAAATGGGTTTTGTACCTATCGATACCCCCGCGTTGGAGTACACCG
AGGTTTTGCTGCGCAAAAGTGAGGGTGACACAGAGAAGCAGATGTTTCGCTTTGTTGATAAGGGTGGAAGAGATGTGGCC
CTCCGCTTTGATCTTACGGTGCCGCTTGCGCGGTTCGTTGCAACGCACTATGCGCGTTTGTATTTTCCTTTTAAGCGCTA
TCATTTTGCAAAAGTGTGGAGGGGCGAGAAGCCTCAGATGGGTCGTTATAGAGAATTCACGCAGTGTGATTTTGATATCG
TCGGTTCGGATTCGGTGTGTGCTGACTTTGAAATTCTAAAGTCGATACGGCACATGTTGTATATGGCTGGTGCAGAACAC
ATACGTATTCACGTTGCGCATCGTGGCCTGTTTGATCGTTTTTTGCGTGCTCTTTCTTTGTCTGACCAGGCTGAGCATAT
CCTGCGGATAATTGACAAACGTGCAAAGATGGCGCCGCATGTGTTGACAGCTCAACTTGAGTCGCTTTGCGATCCAGTTC
GTGTGCAAAAGATTATGACGTATGTAAGTGCGGGGGAGGTGGACGGTGTTGCGCCGTCGTTTGAACATACATTGTCTGCC
ATTGAGACATTGACAGGGGGTGTCTCGGAAGAGAGTACACGGCTTAGAAAAATATATGAGCTACTCTGTGCAGTGAACAT
TCAGTCCTCTTATGTGTTCGATCCATCTATCACGCGTGGTTTTGATTACTACACCGGTATGGTGTGTGAAACGTTTTTAA
CACAGTTGCCTCATATCGGTTCGGTGTGCTCAGGTGGGCGCTATGACCATCTGACGGCTTTGTACATGAAGGATGCAGTG
AGTGGGGTGGGTGCATCCATTGGGTTGGATCGCTTGTATGCAGCGTTTCAGCAGTTGGGAATGTCCCGAGAGCACGTTTG
TTTTGTGCAGGCGCTTATCTTCTGTCAGGATAGTGCGCTCATGGATGTGTACCAAAAGCTGTGTTCATACTTTGCAGTGC
AGGTGGCGACGGAAGTCTTCCCTGATCCGCGGAAGTTGAGCCAACAGTACGCCTTTGCAGAGAAGAAGGGGATTAGGTGG
GGGATCTTTGTTGAACAGCGCAACGCCGTGGTGGAGGACTGCCTGCTCGTACTGCGCGACCTTTCTACGCGAAAGGACAC
ACGCCTACCTGCGCACGAAGTGCGCAGACGCATGGCAGCTGAAGGGTAA

Upstream 100 bases:

>100_bases
CACCACAGGATAGAGGGGCATTGTAATGGGAAGGTGCTGCTCTGTGCAATGCTCACAAAAAGTGCATGTCTTGAAAAAGT
GTACCAGAGCCACTACACTG

Downstream 100 bases:

>100_bases
CAGGCGCCCCCGCGACTCTAGAGTCGCATGTTGCTCAATTCAGTGACTAGGTCCGTTATGGAATCCTTGTTCTTCTGTCC
GATTTCAGTGATTGAGGAAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MRVGSAVSPKVLKGFRDLLPDEEIERALLVEKLTVALRQMGFVPIDTPALEYTEVLLRKSEGDTEKQMFRFVDKGGRDVA
LRFDLTVPLARFVATHYARLYFPFKRYHFAKVWRGEKPQMGRYREFTQCDFDIVGSDSVCADFEILKSIRHMLYMAGAEH
IRIHVAHRGLFDRFLRALSLSDQAEHILRIIDKRAKMAPHVLTAQLESLCDPVRVQKIMTYVSAGEVDGVAPSFEHTLSA
IETLTGGVSEESTRLRKIYELLCAVNIQSSYVFDPSITRGFDYYTGMVCETFLTQLPHIGSVCSGGRYDHLTALYMKDAV
SGVGASIGLDRLYAAFQQLGMSREHVCFVQALIFCQDSALMDVYQKLCSYFAVQVATEVFPDPRKLSQQYAFAEKKGIRW
GIFVEQRNAVVEDCLLVLRDLSTRKDTRLPAHEVRRRMAAEG

Sequences:

>Translated_442_residues
MRVGSAVSPKVLKGFRDLLPDEEIERALLVEKLTVALRQMGFVPIDTPALEYTEVLLRKSEGDTEKQMFRFVDKGGRDVA
LRFDLTVPLARFVATHYARLYFPFKRYHFAKVWRGEKPQMGRYREFTQCDFDIVGSDSVCADFEILKSIRHMLYMAGAEH
IRIHVAHRGLFDRFLRALSLSDQAEHILRIIDKRAKMAPHVLTAQLESLCDPVRVQKIMTYVSAGEVDGVAPSFEHTLSA
IETLTGGVSEESTRLRKIYELLCAVNIQSSYVFDPSITRGFDYYTGMVCETFLTQLPHIGSVCSGGRYDHLTALYMKDAV
SGVGASIGLDRLYAAFQQLGMSREHVCFVQALIFCQDSALMDVYQKLCSYFAVQVATEVFPDPRKLSQQYAFAEKKGIRW
GIFVEQRNAVVEDCLLVLRDLSTRKDTRLPAHEVRRRMAAEG
>Mature_442_residues
MRVGSAVSPKVLKGFRDLLPDEEIERALLVEKLTVALRQMGFVPIDTPALEYTEVLLRKSEGDTEKQMFRFVDKGGRDVA
LRFDLTVPLARFVATHYARLYFPFKRYHFAKVWRGEKPQMGRYREFTQCDFDIVGSDSVCADFEILKSIRHMLYMAGAEH
IRIHVAHRGLFDRFLRALSLSDQAEHILRIIDKRAKMAPHVLTAQLESLCDPVRVQKIMTYVSAGEVDGVAPSFEHTLSA
IETLTGGVSEESTRLRKIYELLCAVNIQSSYVFDPSITRGFDYYTGMVCETFLTQLPHIGSVCSGGRYDHLTALYMKDAV
SGVGASIGLDRLYAAFQQLGMSREHVCFVQALIFCQDSALMDVYQKLCSYFAVQVATEVFPDPRKLSQQYAFAEKKGIRW
GIFVEQRNAVVEDCLLVLRDLSTRKDTRLPAHEVRRRMAAEG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=457, Percent_Identity=29.5404814004376, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI6996014, Length=462, Percent_Identity=30.5194805194805, Blast_Score=158, Evalue=9e-39,
Organism=Escherichia coli, GI1788861, Length=441, Percent_Identity=24.9433106575964, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71993693, Length=451, Percent_Identity=26.6075388026608, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI71993686, Length=451, Percent_Identity=26.6075388026608, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6325290, Length=371, Percent_Identity=30.4582210242588, Blast_Score=147, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24643061, Length=472, Percent_Identity=30.0847457627119, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24643059, Length=490, Percent_Identity=28.5714285714286, Blast_Score=138, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_TREPA (O83647)

Other databases:

- EMBL:   AE000520
- PIR:   H71298
- RefSeq:   NP_219078.1
- ProteinModelPortal:   O83647
- SMR:   O83647
- IntAct:   O83647
- GeneID:   2611765
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0641
- NMPDR:   fig|243276.1.peg.637
- TIGR:   TP_0641
- HOGENOM:   HBG616575
- OMA:   ALDIARC
- ProtClustDB:   PRK00037
- BioCyc:   TPAL243276:TP_0641-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR015807
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: =6.1.1.21

Molecular weight: Translated: 50200; Mature: 50200

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGSAVSPKVLKGFRDLLPDEEIERALLVEKLTVALRQMGFVPIDTPALEYTEVLLRKS
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
EGDTEKQMFRFVDKGGRDVALRFDLTVPLARFVATHYARLYFPFKRYHFAKVWRGEKPQM
CCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRYREFTQCDFDIVGSDSVCADFEILKSIRHMLYMAGAEHIRIHVAHRGLFDRFLRALSL
CCCHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCC
SDQAEHILRIIDKRAKMAPHVLTAQLESLCDPVRVQKIMTYVSAGEVDGVAPSFEHTLSA
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
IETLTGGVSEESTRLRKIYELLCAVNIQSSYVFDPSITRGFDYYTGMVCETFLTQLPHIG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCHHHHHHHHHHHHHHCCCCC
SVCSGGRYDHLTALYMKDAVSGVGASIGLDRLYAAFQQLGMSREHVCFVQALIFCQDSAL
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHH
MDVYQKLCSYFAVQVATEVFPDPRKLSQQYAFAEKKGIRWGIFVEQRNAVVEDCLLVLRD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHH
LSTRKDTRLPAHEVRRRMAAEG
HCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MRVGSAVSPKVLKGFRDLLPDEEIERALLVEKLTVALRQMGFVPIDTPALEYTEVLLRKS
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
EGDTEKQMFRFVDKGGRDVALRFDLTVPLARFVATHYARLYFPFKRYHFAKVWRGEKPQM
CCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRYREFTQCDFDIVGSDSVCADFEILKSIRHMLYMAGAEHIRIHVAHRGLFDRFLRALSL
CCCHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCC
SDQAEHILRIIDKRAKMAPHVLTAQLESLCDPVRVQKIMTYVSAGEVDGVAPSFEHTLSA
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
IETLTGGVSEESTRLRKIYELLCAVNIQSSYVFDPSITRGFDYYTGMVCETFLTQLPHIG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCHHHHHHHHHHHHHHCCCCC
SVCSGGRYDHLTALYMKDAVSGVGASIGLDRLYAAFQQLGMSREHVCFVQALIFCQDSAL
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHH
MDVYQKLCSYFAVQVATEVFPDPRKLSQQYAFAEKKGIRWGIFVEQRNAVVEDCLLVLRD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHH
LSTRKDTRLPAHEVRRRMAAEG
HCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876