Click here to switch to the map view.

The map label for this gene is trpS

Identifier: 189025858

GI number: 189025858

Start: 691687

End: 692700

Strand: Direct

Name: trpS

Synonym: TPASS_0632

Alternate gene names: 189025858

Gene position: 691687-692700 (Clockwise)

Preceding gene: 189025857

Following gene: 189025859

Centisome position: 60.7

GC content: 56.61

Gene sequence:

>1014_bases
ATGCTGCGCGTCCTTACCGGAGACCGGCCCACGGGGCGTCTCCACCTCGGTCACTATGCAGGCTCGCTCGGGACGCGTCT
GCGCATACAGTCTGAGCACGAGTGTTTCTTCATCATCGCGGATCTGCACACGCTCACTACACGCCATGGGCGTGCACAGC
TTGCCGAGCTGCCTGCTCTCGTTCGTGAGCTCGTGCTGGACTACCTAGCTTGTGGCATCGACGCGCAGCGTGCGGTCATT
TATCTGCAATCGGCGGTGCCAGAAGTTACAGAGCTGGCGTTCATTTTTGCGAATCTGGTAACGGTGCCGCGTTTGCAGCG
CATCCCTTCCTTAAAGGAGATGGCTCAAGCGGCAAACCTTTCTGAACTTCCCTTTGGTCTTTTGGGATATCCCGTACTTC
AAGCGGCGGACATTCTGCTGGCGCGGGCGCATCTTGTGCCGGTAGGTAAAGATAATGAAAGTCACGTAGAACTGACGCGC
GAAGTTGCAAAGCGTTTCAATCGCCTCTATGGGGAGGTCTTTCCATTGCCGCGTGCACTCCTCAGTGACTGCGCCACTCT
CGTGGGAACTGACGGACAAGCGAAAATGTCTAAAAGTTTGGGGAACGCGATTTTTCTCTCGGACGATGAAGAGACGGTGC
GGCGCAAGGTCCGTGCGATGTACACAGATCCTGCGCGCACCCGGGCAGATATACCAGGACGCGTGGAAGGGAATCCGGTG
TTTGCGTACCACGATGCGTTCAACCCAAACACGGACGAGGTTGCACATTTTAAAGAGCGGTATCGCGCTGGGCGCGTGGG
AGACACAGAAGTGAAAGACGCTCTTGCGCGCGCCTTGAATACGTTTTTAGAACCCATCCGCGCACGCCGTGCACAGTTAG
AACAGCAGCCTGCGTACCTTGCACGCGTATTAGAGGAAGGCTGCGCGCGCATGCGTGCTGAAGCACGCAAAACCATGCAG
AGAGTAAAAGAGGCGATGGGTCTTTCTGCACGTCCTCTCGTTCATCTGCCGTAG

Upstream 100 bases:

>100_bases
GACACGCACCCGTGATATAGAGGAGGGCTGCGCGGTCGGCGCACGTGTTCCTGCGCCTGCACTCTTGAATACTGGACGGA
CCTTGATTTACAGTCTCCCC

Downstream 100 bases:

>100_bases
GGAACGCTTGTAGCCGCGCATCAACACGGCTGCCTGCAACGCAATCACGCTTCCGCCGGTTTCCCTCTTACCAGTGCGCC
GAGTCCTGCCGCCGAGGAGG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MLRVLTGDRPTGRLHLGHYAGSLGTRLRIQSEHECFFIIADLHTLTTRHGRAQLAELPALVRELVLDYLACGIDAQRAVI
YLQSAVPEVTELAFIFANLVTVPRLQRIPSLKEMAQAANLSELPFGLLGYPVLQAADILLARAHLVPVGKDNESHVELTR
EVAKRFNRLYGEVFPLPRALLSDCATLVGTDGQAKMSKSLGNAIFLSDDEETVRRKVRAMYTDPARTRADIPGRVEGNPV
FAYHDAFNPNTDEVAHFKERYRAGRVGDTEVKDALARALNTFLEPIRARRAQLEQQPAYLARVLEEGCARMRAEARKTMQ
RVKEAMGLSARPLVHLP

Sequences:

>Translated_337_residues
MLRVLTGDRPTGRLHLGHYAGSLGTRLRIQSEHECFFIIADLHTLTTRHGRAQLAELPALVRELVLDYLACGIDAQRAVI
YLQSAVPEVTELAFIFANLVTVPRLQRIPSLKEMAQAANLSELPFGLLGYPVLQAADILLARAHLVPVGKDNESHVELTR
EVAKRFNRLYGEVFPLPRALLSDCATLVGTDGQAKMSKSLGNAIFLSDDEETVRRKVRAMYTDPARTRADIPGRVEGNPV
FAYHDAFNPNTDEVAHFKERYRAGRVGDTEVKDALARALNTFLEPIRARRAQLEQQPAYLARVLEEGCARMRAEARKTMQ
RVKEAMGLSARPLVHLP
>Mature_337_residues
MLRVLTGDRPTGRLHLGHYAGSLGTRLRIQSEHECFFIIADLHTLTTRHGRAQLAELPALVRELVLDYLACGIDAQRAVI
YLQSAVPEVTELAFIFANLVTVPRLQRIPSLKEMAQAANLSELPFGLLGYPVLQAADILLARAHLVPVGKDNESHVELTR
EVAKRFNRLYGEVFPLPRALLSDCATLVGTDGQAKMSKSLGNAIFLSDDEETVRRKVRAMYTDPARTRADIPGRVEGNPV
FAYHDAFNPNTDEVAHFKERYRAGRVGDTEVKDALARALNTFLEPIRARRAQLEQQPAYLARVLEEGCARMRAEARKTMQ
RVKEAMGLSARPLVHLP

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=337, Percent_Identity=33.8278931750742, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI41352700, Length=193, Percent_Identity=40.4145077720207, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1789786, Length=337, Percent_Identity=31.7507418397626, Blast_Score=174, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI71982800, Length=335, Percent_Identity=28.3582089552239, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71982793, Length=335, Percent_Identity=28.3582089552239, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320474, Length=356, Percent_Identity=29.7752808988764, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24666151, Length=339, Percent_Identity=33.0383480825959, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_TREPA (O83640)

Other databases:

- EMBL:   AE000520
- PIR:   F71300
- RefSeq:   NP_219070.1
- ProteinModelPortal:   O83640
- SMR:   O83640
- IntAct:   O83640
- GeneID:   2611479
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0632
- NMPDR:   fig|243276.1.peg.629
- TIGR:   TP_0632
- HOGENOM:   HBG293263
- OMA:   TYLDAFH
- ProtClustDB:   PRK00927
- BioCyc:   TPAL243276:TP_0632-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 37563; Mature: 37563

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVLTGDRPTGRLHLGHYAGSLGTRLRIQSEHECFFIIADLHTLTTRHGRAQLAELPAL
CEEEECCCCCCCEEEECHHHCCCCCEEEEECCCCEEEEEEHHHHHHHCCCHHHHHHHHHH
VRELVLDYLACGIDAQRAVIYLQSAVPEVTELAFIFANLVTVPRLQRIPSLKEMAQAANL
HHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
SELPFGLLGYPVLQAADILLARAHLVPVGKDNESHVELTREVAKRFNRLYGEVFPLPRAL
CCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LSDCATLVGTDGQAKMSKSLGNAIFLSDDEETVRRKVRAMYTDPARTRADIPGRVEGNPV
HHHHHHHHCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEE
FAYHDAFNPNTDEVAHFKERYRAGRVGDTEVKDALARALNTFLEPIRARRAQLEQQPAYL
EEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARVLEEGCARMRAEARKTMQRVKEAMGLSARPLVHLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLRVLTGDRPTGRLHLGHYAGSLGTRLRIQSEHECFFIIADLHTLTTRHGRAQLAELPAL
CEEEECCCCCCCEEEECHHHCCCCCEEEEECCCCEEEEEEHHHHHHHCCCHHHHHHHHHH
VRELVLDYLACGIDAQRAVIYLQSAVPEVTELAFIFANLVTVPRLQRIPSLKEMAQAANL
HHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
SELPFGLLGYPVLQAADILLARAHLVPVGKDNESHVELTREVAKRFNRLYGEVFPLPRAL
CCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LSDCATLVGTDGQAKMSKSLGNAIFLSDDEETVRRKVRAMYTDPARTRADIPGRVEGNPV
HHHHHHHHCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEE
FAYHDAFNPNTDEVAHFKERYRAGRVGDTEVKDALARALNTFLEPIRARRAQLEQQPAYL
EEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARVLEEGCARMRAEARKTMQRVKEAMGLSARPLVHLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876