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The map label for this gene is lytB

Identifier: 189025775

GI number: 189025775

Start: 591843

End: 592973

Strand: Reverse

Name: lytB

Synonym: TPASS_0547

Alternate gene names: 189025775

Gene position: 592973-591843 (Counterclockwise)

Preceding gene: 189025778

Following gene: 189025774

Centisome position: 52.04

GC content: 64.37

Gene sequence:

>1131_bases
ATGAGAAAGGTCAATTGCATTAAGGTTATCACGCGCGCCCACACCCTCGGCTATTGCGGCGGGGTGCGTATGGCGGTGCG
CATGGCAGAACACGCCCGCGCCCACCACCGCGGGAGCGTCTACACGCTCGGTCCGCTCGTCCACAATCCCGTGACGCTCG
CCCGTTTGCGCGCGCGTGGCATTGAGTGTCTGGATCCTGCTCATCTATCTTTTGCGCTGCACGCTCCTGCGGCACCGGGC
GCAGCGCCGCATGCAGTGGAAGAAAAGACGGCGCGTACCGTGGTGATTAGAGCGCATGGCGTGGCACCTGAGGTGTATGA
GGCCCTCGAGCGTTCCGGAGCGCAGGTGGTGGACGCCACCTGCCCGCGAGTTAAGGAAAGTCAGCGGCGTGCTCAGGGTT
TTGCCGCGCAGGGACTGCACGTTATTCTCGCCGGGGACCGCAATCATGGGGAAATCGTTGGCATCGAGGGGTATGTGCGC
GCGGGAGCTGCGCAGGCGTGCAGCCACTTGCCGGGCGGCGCACCAGACGGCATGCTGCCACAGGTGCAGTGCTTTGTGGT
GCAAAACGCGCGTGAGGCTGCCGCGTTGCCGTGTTTAGCGCGTGCAGCGCTCCTTGCCCAAACTACCATTACACAGGGTG
AATACGACGCGATCGCCGCTGCGGCGCGTACCCGTGTGCGGGAATTGACGGTAGCGCGTACTATCTGCGCGGCAACTGCG
CGCAGACAGGCGGCGCTGCGTGCTCTTGCGCCCACGGTGGAAGCGCTCCTGGTGATCGGCGGCGCACACTCGGCAAATAC
CCAGCGTCTACTCCACACCGCGCGCGAAACGTCGCTGCCTACGTGGCTGGTAGAGCGTGTAGAAGATATTCCCCCCGATA
TCTATGCCTTCAGTGCGGTGGGCATCAGTGCAGGGGCTTCCACCCCAGACTGTGTTATCGCTGCTGTGGAGCAGGCGCTG
CGCACGGGCGGCGCGCCTGTCGCTTCTCGGGTGTCTTCCTCTGCTCTGCCCAAGGTGAGTACCTGCAGGGCTGTTTGTGC
GGCGGCTACTTCTTCCGTCGGTTCAGCGGGTGCATCCGGCGCGGTGTCGCCCGGTGCTGTCCGACCTTTTGCTGTAGGCT
CCGTGCGGTGA

Upstream 100 bases:

>100_bases
AAGAAGAACTCACCACCACGCCGCGCCCTTGCATGACCTGGGTGCCTTTGGTACACTCGGCGCGCCAGTTTTCGTCCGGT
TTAGTGCCCCAGTGAGTGAT

Downstream 100 bases:

>100_bases
AAACCGCTTCCCTTTGTCTGCGAAGTGCATGGCTGAACGTTGCGCTCGGCGGGGTGCTGGGTGTCCTTTCCGGTTGTCGT
GTCTTCGACGAAAGGGCGGA

Product: penicillin tolerance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRKVNCIKVITRAHTLGYCGGVRMAVRMAEHARAHHRGSVYTLGPLVHNPVTLARLRARGIECLDPAHLSFALHAPAAPG
AAPHAVEEKTARTVVIRAHGVAPEVYEALERSGAQVVDATCPRVKESQRRAQGFAAQGLHVILAGDRNHGEIVGIEGYVR
AGAAQACSHLPGGAPDGMLPQVQCFVVQNAREAAALPCLARAALLAQTTITQGEYDAIAAAARTRVRELTVARTICAATA
RRQAALRALAPTVEALLVIGGAHSANTQRLLHTARETSLPTWLVERVEDIPPDIYAFSAVGISAGASTPDCVIAAVEQAL
RTGGAPVASRVSSSALPKVSTCRAVCAAATSSVGSAGASGAVSPGAVRPFAVGSVR

Sequences:

>Translated_376_residues
MRKVNCIKVITRAHTLGYCGGVRMAVRMAEHARAHHRGSVYTLGPLVHNPVTLARLRARGIECLDPAHLSFALHAPAAPG
AAPHAVEEKTARTVVIRAHGVAPEVYEALERSGAQVVDATCPRVKESQRRAQGFAAQGLHVILAGDRNHGEIVGIEGYVR
AGAAQACSHLPGGAPDGMLPQVQCFVVQNAREAAALPCLARAALLAQTTITQGEYDAIAAAARTRVRELTVARTICAATA
RRQAALRALAPTVEALLVIGGAHSANTQRLLHTARETSLPTWLVERVEDIPPDIYAFSAVGISAGASTPDCVIAAVEQAL
RTGGAPVASRVSSSALPKVSTCRAVCAAATSSVGSAGASGAVSPGAVRPFAVGSVR
>Mature_376_residues
MRKVNCIKVITRAHTLGYCGGVRMAVRMAEHARAHHRGSVYTLGPLVHNPVTLARLRARGIECLDPAHLSFALHAPAAPG
AAPHAVEEKTARTVVIRAHGVAPEVYEALERSGAQVVDATCPRVKESQRRAQGFAAQGLHVILAGDRNHGEIVGIEGYVR
AGAAQACSHLPGGAPDGMLPQVQCFVVQNAREAAALPCLARAALLAQTTITQGEYDAIAAAARTRVRELTVARTICAATA
RRQAALRALAPTVEALLVIGGAHSANTQRLLHTARETSLPTWLVERVEDIPPDIYAFSAVGISAGASTPDCVIAAVEQAL
RTGGAPVASRVSSSALPKVSTCRAVCAAATSSVGSAGASGAVSPGAVRPFAVGSVR

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=315, Percent_Identity=27.9365079365079, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_TREPA (O83558)

Other databases:

- EMBL:   AE000520
- PIR:   G71310
- RefSeq:   NP_218986.1
- ProteinModelPortal:   O83558
- IntAct:   O83558
- GeneID:   2611796
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0547
- NMPDR:   fig|243276.1.peg.545
- TIGR:   TP_0547
- HOGENOM:   HBG335228
- OMA:   REENIEF
- ProtClustDB:   CLSK218751
- BioCyc:   TPAL243276:TP_0547-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 38885; Mature: 38885

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVNCIKVITRAHTLGYCGGVRMAVRMAEHARAHHRGSVYTLGPLVHNPVTLARLRARG
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHCC
IECLDPAHLSFALHAPAAPGAAPHAVEEKTARTVVIRAHGVAPEVYEALERSGAQVVDAT
CCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHCCCEEEECC
CPRVKESQRRAQGFAAQGLHVILAGDRNHGEIVGIEGYVRAGAAQACSHLPGGAPDGMLP
CCHHHHHHHHHHCHHHCCEEEEEECCCCCCCEEEEHHHHHCCHHHHHHCCCCCCCCCCCC
QVQCFVVQNAREAAALPCLARAALLAQTTITQGEYDAIAAAARTRVRELTVARTICAATA
CEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRQAALRALAPTVEALLVIGGAHSANTQRLLHTARETSLPTWLVERVEDIPPDIYAFSAV
HHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHH
GISAGASTPDCVIAAVEQALRTGGAPVASRVSSSALPKVSTCRAVCAAATSSVGSAGASG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
AVSPGAVRPFAVGSVR
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKVNCIKVITRAHTLGYCGGVRMAVRMAEHARAHHRGSVYTLGPLVHNPVTLARLRARG
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHCC
IECLDPAHLSFALHAPAAPGAAPHAVEEKTARTVVIRAHGVAPEVYEALERSGAQVVDAT
CCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHCCCEEEECC
CPRVKESQRRAQGFAAQGLHVILAGDRNHGEIVGIEGYVRAGAAQACSHLPGGAPDGMLP
CCHHHHHHHHHHCHHHCCEEEEEECCCCCCCEEEEHHHHHCCHHHHHHCCCCCCCCCCCC
QVQCFVVQNAREAAALPCLARAALLAQTTITQGEYDAIAAAARTRVRELTVARTICAATA
CEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRQAALRALAPTVEALLVIGGAHSANTQRLLHTARETSLPTWLVERVEDIPPDIYAFSAV
HHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHH
GISAGASTPDCVIAAVEQALRTGGAPVASRVSSSALPKVSTCRAVCAAATSSVGSAGASG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
AVSPGAVRPFAVGSVR
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876