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The map label for this gene is tpiA

Identifier: 189025765

GI number: 189025765

Start: 580487

End: 581236

Strand: Reverse

Name: tpiA

Synonym: TPASS_0537

Alternate gene names: 189025765

Gene position: 581236-580487 (Counterclockwise)

Preceding gene: 189025766

Following gene: 189025764

Centisome position: 51.01

GC content: 56.27

Gene sequence:

>750_bases
ATGCGCGGCTATTTTATCGCGGGGAATTGGAAGATGCATAAAACGTGTGCGGAGGCGGTGGCGCTTGCGCAGGAACTCGT
GCGGGAATTACGGGGTGGTCCGCACACATACATGATTGCGCCGAGTTTTACCGCTTTAGACGCGGTGGGGAAGGTGCTAC
GGGGAAGTAACGTACTTCTTGGCGCGCAGGACGTGAGTAGTGAAGAGTGGGGGGCGCATACTGGAGAGGTATCCGTCCTT
CAACTCGAAGACCTGGGGGTACAGGTAGTCATCGTGGGGCATTCCGAACGGCGTCATGGGCGTGGGGAGAATGATAAGCT
TATCAATCAAAAGGTCAGACGCGTGTTAGAAAGCGGTTTGCGCGTCATCTTATGCGTCGGCGAACGACTCCAAGAGTATG
AAGCGGGGTGTACCAACGAGGTAGTGGGAACTCAAGTGCGCGCAGGGATGGCAGACGTGTGTGGGTCACTCATGCATAAT
GTAACTGTTGCGTATGAGCCTGTGTGGGCAATTGGTACGGGTAAGACTGCCACTCCGGCACAGGCGAATGCGGTTCATGC
TCATATTCGGTCAGTAGTCCGCGAGATGTACGGCGCGGCTATCGCAGAGGCACTGTGTATTCAATACGGCGGATCCATGA
AAGCGGAGAACGCGCGGGCACTGTTGGCTGAAGAGCACATTGACGGGGGACTCATTGGCGGTGCTTCGCTGGAGGCTGCG
TCTTTTGTTCCTATCGCGCGCAGCGTGTAG

Upstream 100 bases:

>100_bases
GCGAGGGTATGTCTCTGCGGTGGCTCTATAGAACAGTTTCTCGTGGAGCTATCGGCGGGGTGTTTGTGGTGTGAACGAAA
GGATGTGCTAAGGAGGGGAT

Downstream 100 bases:

>100_bases
AGAGTAGTAAAGGATTCTGGGTGGTGTGCGGTATTGCGTAAGCAGGTTTCTTTTTTTGTGCCGTTCACATGGTCGTTTTT
CTGTTCTCGGGACAGGGAGT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRGYFIAGNWKMHKTCAEAVALAQELVRELRGGPHTYMIAPSFTALDAVGKVLRGSNVLLGAQDVSSEEWGAHTGEVSVL
QLEDLGVQVVIVGHSERRHGRGENDKLINQKVRRVLESGLRVILCVGERLQEYEAGCTNEVVGTQVRAGMADVCGSLMHN
VTVAYEPVWAIGTGKTATPAQANAVHAHIRSVVREMYGAAIAEALCIQYGGSMKAENARALLAEEHIDGGLIGGASLEAA
SFVPIARSV

Sequences:

>Translated_249_residues
MRGYFIAGNWKMHKTCAEAVALAQELVRELRGGPHTYMIAPSFTALDAVGKVLRGSNVLLGAQDVSSEEWGAHTGEVSVL
QLEDLGVQVVIVGHSERRHGRGENDKLINQKVRRVLESGLRVILCVGERLQEYEAGCTNEVVGTQVRAGMADVCGSLMHN
VTVAYEPVWAIGTGKTATPAQANAVHAHIRSVVREMYGAAIAEALCIQYGGSMKAENARALLAEEHIDGGLIGGASLEAA
SFVPIARSV
>Mature_249_residues
MRGYFIAGNWKMHKTCAEAVALAQELVRELRGGPHTYMIAPSFTALDAVGKVLRGSNVLLGAQDVSSEEWGAHTGEVSVL
QLEDLGVQVVIVGHSERRHGRGENDKLINQKVRRVLESGLRVILCVGERLQEYEAGCTNEVVGTQVRAGMADVCGSLMHN
VTVAYEPVWAIGTGKTATPAQANAVHAHIRSVVREMYGAAIAEALCIQYGGSMKAENARALLAEEHIDGGLIGGASLEAA
SFVPIARSV

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=244, Percent_Identity=40.5737704918033, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI226529917, Length=244, Percent_Identity=40.5737704918033, Blast_Score=158, Evalue=5e-39,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=44.1767068273092, Blast_Score=190, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=43.7751004016064, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320255, Length=251, Percent_Identity=41.0358565737052, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=42.6829268292683, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=42.6829268292683, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28572004, Length=246, Percent_Identity=42.6829268292683, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_TREPA (O83548)

Other databases:

- EMBL:   AE000520
- PIR:   F71311
- RefSeq:   NP_218976.1
- ProteinModelPortal:   O83548
- SMR:   O83548
- IntAct:   O83548
- GeneID:   2611705
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0537
- NMPDR:   fig|243276.1.peg.535
- TIGR:   TP_0537
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   TPAL243276:TP_0537-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26541; Mature: 26541

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 94-94 ACT_SITE 166-166 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGYFIAGNWKMHKTCAEAVALAQELVRELRGGPHTYMIAPSFTALDAVGKVLRGSNVLL
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCEEE
GAQDVSSEEWGAHTGEVSVLQLEDLGVQVVIVGHSERRHGRGENDKLINQKVRRVLESGL
ECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHCC
RVILCVGERLQEYEAGCTNEVVGTQVRAGMADVCGSLMHNVTVAYEPVWAIGTGKTATPA
EEEEHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCEEEECCEEEECCCCCCCCC
QANAVHAHIRSVVREMYGAAIAEALCIQYGGSMKAENARALLAEEHIDGGLIGGASLEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEECCCCCCHH
SFVPIARSV
HCCCHHCCC
>Mature Secondary Structure
MRGYFIAGNWKMHKTCAEAVALAQELVRELRGGPHTYMIAPSFTALDAVGKVLRGSNVLL
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCEEE
GAQDVSSEEWGAHTGEVSVLQLEDLGVQVVIVGHSERRHGRGENDKLINQKVRRVLESGL
ECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHCC
RVILCVGERLQEYEAGCTNEVVGTQVRAGMADVCGSLMHNVTVAYEPVWAIGTGKTATPA
EEEEHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCEEEECCEEEECCCCCCCCC
QANAVHAHIRSVVREMYGAAIAEALCIQYGGSMKAENARALLAEEHIDGGLIGGASLEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEECCCCCCHH
SFVPIARSV
HCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876