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The map label for this gene is pcnA [H]

Identifier: 189025504

GI number: 189025504

Start: 287287

End: 288723

Strand: Direct

Name: pcnA [H]

Synonym: TPASS_0270

Alternate gene names: 189025504

Gene position: 287287-288723 (Clockwise)

Preceding gene: 189025503

Following gene: 189025507

Centisome position: 25.21

GC content: 54.84

Gene sequence:

>1437_bases
GTGCGCGTGTTTCCTGTCTGTCCGCTGCTGCGTGAAGTGAGTGAGCGTTTCATCCGTGCAGGATTTTGCATTTATGTTGT
CGGGGGTGCTGTGCGTGACTTTCTCCTGAACCGCAGTGCTCATGATTGGGATCTTGCAACTGACGCTCCCCCTGAGCGTG
TGCGTATGCTTTTTAGACGCACGGTTCCTACTGGTATTGAACACGGCACTGTTACAATTCTTTTCAGAGGGCATTCTATT
GAGTGCACTACGTTCCGTGTTGAGTCGGATTATTCCGATAGGAGACATCCGGATTCTGTTTGCTTTGCCGCGCGCATTGA
GGACGATTTGGCAAGGCGCGACTTCACTGTCAATGCTTTCGCTGCCGCGCTCCCCTCGGGGGAAATCATCGACGTATGTG
GCGGTTACGCCGATTTGCGTAACGGTCTTATCTGTAGCGTTGGGGATGCACATGCTAGATTTTCTGAAGATGCGTTGCGT
CCTTTGCGTGCTGTGCGTTTTGCAGCGCAGTTATCTTTTTCCATCGAAGCGCGCACGCGTGAAGCAATTATCGCGCTACG
CGCTCATACTGCACGTATTTCTCGTGAGCGGGTGCGTGATGAACTTTCTAAGATGCTTTGTACTCCCCGTCCGAGTATTG
CGCTCCGCCTAATGGAAGAGACTGGATTGCTGCACACACTTTTTCCTGCGCTGGCGCAGGTGTGTGGGAGAAACGAAGGG
GAGGAGAAGGAGACGCAGGACAGTCTGCAGGTTTCACCGCAGCAACGACGTGACGCTCGCACCTTTGCTGCGTGCGCGTT
TGCTGCGTGCGATCGGGTGCCTGCAGAGCTTGCGGTCCGCCTTGCAGCACTTCTCTTTCCGCTGGCTCACTATCGTACGC
TTCCTGCTTCAGGAACGGGGGCAGCGTTGTTTATATGCCCTCCTGCACTTGCTGAAGCGCGCGAGCTTTTGCGTGGCCTG
AAGTACCCGAATTACCTGACGGCTCAGGTGTGTCACTTGGTTGCACACGCGCGTTTTACTCCACACGAATGTTGGTCTGA
AGGAACGCTCCGTCGTTTCGTTGTGACGGTAGGTACTACTCAGCTAGAATCGCTGTGTGTCCTGCGGCGTGCCTATCTTG
CTGATGACGATTACCGCATATCTGCTCAGCCTGGAGTGTCCTGCGAGAATGCGCGGGAAGGAAAAAAAATGCAGGAACAG
TTTGAAATATTCGTAACACGCGTGCGCAGGGTCGCCGCACAATTGCCTGTGCACGGCATACGCGACCTTGCGGTGAACGG
ACGCGACTTGATAGCGCAGGGCATTCCCCCAGGTCCCACCATAGGGCACATCCTGAACGCACTGTTTGATATGGTCCTCA
TGCAACCGTCGCGCAACACGCGTCCGCAGCTTTTGGAACATGCGCAGGAGATTGTGCGCACCATGGCGCAGAATTAG

Upstream 100 bases:

>100_bases
CATAACGAGGGTGCTATCCTCTTGTGAGTTTCTGATTCGCAGTGGAGATTCATTGTTGTTAGAAAAAAACGAGCATGCAG
TAGAGCGTGAGGAAGGGTCT

Downstream 100 bases:

>100_bases
TGCGCCTTGAGCAACCCGTAGAGTCTTTCTAATTCTGCTTGGGAGAAATACGTGATCTCAATTCTCCCCCGTTGTAAATT
CCCACTGATGCGCACCTTGG

Product: polynucleotide adenylyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRVFPVCPLLREVSERFIRAGFCIYVVGGAVRDFLLNRSAHDWDLATDAPPERVRMLFRRTVPTGIEHGTVTILFRGHSI
ECTTFRVESDYSDRRHPDSVCFAARIEDDLARRDFTVNAFAAALPSGEIIDVCGGYADLRNGLICSVGDAHARFSEDALR
PLRAVRFAAQLSFSIEARTREAIIALRAHTARISRERVRDELSKMLCTPRPSIALRLMEETGLLHTLFPALAQVCGRNEG
EEKETQDSLQVSPQQRRDARTFAACAFAACDRVPAELAVRLAALLFPLAHYRTLPASGTGAALFICPPALAEARELLRGL
KYPNYLTAQVCHLVAHARFTPHECWSEGTLRRFVVTVGTTQLESLCVLRRAYLADDDYRISAQPGVSCENAREGKKMQEQ
FEIFVTRVRRVAAQLPVHGIRDLAVNGRDLIAQGIPPGPTIGHILNALFDMVLMQPSRNTRPQLLEHAQEIVRTMAQN

Sequences:

>Translated_478_residues
MRVFPVCPLLREVSERFIRAGFCIYVVGGAVRDFLLNRSAHDWDLATDAPPERVRMLFRRTVPTGIEHGTVTILFRGHSI
ECTTFRVESDYSDRRHPDSVCFAARIEDDLARRDFTVNAFAAALPSGEIIDVCGGYADLRNGLICSVGDAHARFSEDALR
PLRAVRFAAQLSFSIEARTREAIIALRAHTARISRERVRDELSKMLCTPRPSIALRLMEETGLLHTLFPALAQVCGRNEG
EEKETQDSLQVSPQQRRDARTFAACAFAACDRVPAELAVRLAALLFPLAHYRTLPASGTGAALFICPPALAEARELLRGL
KYPNYLTAQVCHLVAHARFTPHECWSEGTLRRFVVTVGTTQLESLCVLRRAYLADDDYRISAQPGVSCENAREGKKMQEQ
FEIFVTRVRRVAAQLPVHGIRDLAVNGRDLIAQGIPPGPTIGHILNALFDMVLMQPSRNTRPQLLEHAQEIVRTMAQN
>Mature_478_residues
MRVFPVCPLLREVSERFIRAGFCIYVVGGAVRDFLLNRSAHDWDLATDAPPERVRMLFRRTVPTGIEHGTVTILFRGHSI
ECTTFRVESDYSDRRHPDSVCFAARIEDDLARRDFTVNAFAAALPSGEIIDVCGGYADLRNGLICSVGDAHARFSEDALR
PLRAVRFAAQLSFSIEARTREAIIALRAHTARISRERVRDELSKMLCTPRPSIALRLMEETGLLHTLFPALAQVCGRNEG
EEKETQDSLQVSPQQRRDARTFAACAFAACDRVPAELAVRLAALLFPLAHYRTLPASGTGAALFICPPALAEARELLRGL
KYPNYLTAQVCHLVAHARFTPHECWSEGTLRRFVVTVGTTQLESLCVLRRAYLADDDYRISAQPGVSCENAREGKKMQEQ
FEIFVTRVRRVAAQLPVHGIRDLAVNGRDLIAQGIPPGPTIGHILNALFDMVLMQPSRNTRPQLLEHAQEIVRTMAQN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=33.9805825242718, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI87081691, Length=237, Percent_Identity=34.5991561181435, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789436, Length=227, Percent_Identity=33.920704845815, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71995920, Length=475, Percent_Identity=25.4736842105263, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321016, Length=258, Percent_Identity=28.2945736434109, Blast_Score=90, Evalue=9e-19,
Organism=Drosophila melanogaster, GI281360112, Length=209, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357337, Length=209, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 53321; Mature: 53321

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFPVCPLLREVSERFIRAGFCIYVVGGAVRDFLLNRSAHDWDLATDAPPERVRMLFRR
CCCCCCCHHHHHHHHHHHHCCHHEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
TVPTGIEHGTVTILFRGHSIECTTFRVESDYSDRRHPDSVCFAARIEDDLARRDFTVNAF
HCCCCCCCCEEEEEEECCEEEEEEEEEECCCCCCCCCCHHEEHHHHHHHHHHCCCCHHHH
AAALPSGEIIDVCGGYADLRNGLICSVGDAHARFSEDALRPLRAVRFAAQLSFSIEARTR
HHHCCCCCEEHHCCCHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHH
EAIIALRAHTARISRERVRDELSKMLCTPRPSIALRLMEETGLLHTLFPALAQVCGRNEG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
EEKETQDSLQVSPQQRRDARTFAACAFAACDRVPAELAVRLAALLFPLAHYRTLPASGTG
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
AALFICPPALAEARELLRGLKYPNYLTAQVCHLVAHARFTPHECWSEGTLRRFVVTVGTT
CEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHEEEEECHH
QLESLCVLRRAYLADDDYRISAQPGVSCENAREGKKMQEQFEIFVTRVRRVAAQLPVHGI
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RDLAVNGRDLIAQGIPPGPTIGHILNALFDMVLMQPSRNTRPQLLEHAQEIVRTMAQN
HHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVFPVCPLLREVSERFIRAGFCIYVVGGAVRDFLLNRSAHDWDLATDAPPERVRMLFRR
CCCCCCCHHHHHHHHHHHHCCHHEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
TVPTGIEHGTVTILFRGHSIECTTFRVESDYSDRRHPDSVCFAARIEDDLARRDFTVNAF
HCCCCCCCCEEEEEEECCEEEEEEEEEECCCCCCCCCCHHEEHHHHHHHHHHCCCCHHHH
AAALPSGEIIDVCGGYADLRNGLICSVGDAHARFSEDALRPLRAVRFAAQLSFSIEARTR
HHHCCCCCEEHHCCCHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHH
EAIIALRAHTARISRERVRDELSKMLCTPRPSIALRLMEETGLLHTLFPALAQVCGRNEG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
EEKETQDSLQVSPQQRRDARTFAACAFAACDRVPAELAVRLAALLFPLAHYRTLPASGTG
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
AALFICPPALAEARELLRGLKYPNYLTAQVCHLVAHARFTPHECWSEGTLRRFVVTVGTT
CEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHEEEEECHH
QLESLCVLRRAYLADDDYRISAQPGVSCENAREGKKMQEQFEIFVTRVRRVAAQLPVHGI
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RDLAVNGRDLIAQGIPPGPTIGHILNALFDMVLMQPSRNTRPQLLEHAQEIVRTMAQN
HHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA