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The map label for this gene is cysS

Identifier: 189025325

GI number: 189025325

Start: 99986

End: 101548

Strand: Direct

Name: cysS

Synonym: TPASS_0091

Alternate gene names: 189025325

Gene position: 99986-101548 (Clockwise)

Preceding gene: 189025320

Following gene: 189025326

Centisome position: 8.77

GC content: 54.51

Gene sequence:

>1563_bases
ATGGCCTTGCGCGTATACAACACCCTTACTCGTCAGCAAGAGCACTTTCAACCCTGGGAGCACGGGCACGTGCGTCTCTA
CGGTTGTGGGCCTACGGTGTACAATTATCCCCATCTGGGGAATCTGCGCGCATACGTTTTTCAGGATACGGTTCGACGTA
CCTTGCACTTTCTTGGATACCGCGTCACCTACGTTATGAATATTACCGACGTTGGGCATTTAGAAAGTGACGCAGACAGT
GGTGAGGATAAGCTGGTAAGGAGCGCACAGGCGCATGGCCACTCGGTGTTGCAGGTTGCAGCGCACTATCGCGCAGCCTT
TTTCCGCGATACTGCACTGCTCGGTATTGAAGAGCCGTCCATTGTCTGTAATGCCAGCGATTGTATCCAGGATATGATCG
CGTTTATCGAGCAATTGCTCGCGCGTGGGCACGCGTACTGTGCAGGAGGGAACGTGTATTTTGATGTGCGATCCTTTCCT
AGCTACGAAAGCTTCGGTTCTGCCGCGGTAGAAGATGTTCAGGAAGGAGAGGATGCGGCGCGCGCGCGGGTGGCACACGA
TACGCATAAGCGTGATGCACGTGATTTTGTGCTGTGGTTTACCCGTAGTAAATTTGTGCGTCATGCGTTGACGTGGGATT
CTCCGTGGGGGCGGGGGTACCCCGGGTGGCACATCGGGTGTTCTGCAATGAGCATGAAGTTTTTAGGACCACGTTGCGAC
ATCCACATCGGAGGGGTGGATCATATTCGTGTGCATCACCGTAACGAGCGTGCTCAGTGTGAAGCAATTACTGGTGCACC
CTGGGTGAGGTACTGGTTACACCACGAGTTCTTGCTGATGCAGCTGCAAAAGCGCGCAGTACATGCGGATATGGGCAGTT
CGGTGGTGTCGTCTTTTTCTAAAATGTCCAAGTCCTGTGGGCAGTTTTTGACGCTTTCTTCGCTGCAGGAGCGTGGCTTT
CAGCCAGCTGATTTTCGCTTCTTTTTGTTGAGTGGACAGTATCGCACGCAACTTGCTTTTTCTTGGGATGCGCTAAAAAC
GGCGCGTGCCGCCCGACGGAGTTTTGTGCGGCGAGTGGCGCGTGTAGTGGACGCTGCTCGAGCAACTACAGGCAGCGTGC
GCGGCACTAGTGCAGAGTGTGCCGCAGAAAGGGTGTGTGAATCGCGCGCATCAGAATCTGAGCTGCTCTTAACTGACTTT
CGTGCTGCGTTGGAGGATGACTTTTCTACGCCACGTGCTCTGAGCGCCTTACAAAAATTGGTGCGTGATACCTCGGTGCC
GCCATCGCTGTGTGTTTCGGCACTCCAGGTGGCGGATACAGTGCTAGGGTTAGGCATAATACAGGAAGCGACCGCATCGC
TATCTGCGCAGGTTCCTGCTGGCGATACGTTGCCGCAGCGTCCTTTACCGAGTGAGGAGTGGATTGGACAGTTGGTGCGT
GCGCGTGCACATGCACGCCAAACGCGTGATTTTCCCCGTGCAGATGAGATCCGTCGGCAGTTGAAGGCTGAAGGGATTGA
ACTTGAAGACACCCATCTTGGGACTATTTGGAAGCGCGTGTAA

Upstream 100 bases:

>100_bases
ACTGAAGCGCAGGCGGGCAAAGGCGTTTGCGCGACAATGGGGCTGGGCGTGGTCCGCACGTGTTCTGCGCCCGGTTGGGA
GAAACTTCGAAGGGGCGCAC

Downstream 100 bases:

>100_bases
CATTTTGGGAGATACATTGTTGCATGAGCAGGAGCTTTTAAGAGCACAGGATGATGCAGATTTTAAGCTCATGTACGAGC
AGCTTGTGCCAGTGCTCTAC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MALRVYNTLTRQQEHFQPWEHGHVRLYGCGPTVYNYPHLGNLRAYVFQDTVRRTLHFLGYRVTYVMNITDVGHLESDADS
GEDKLVRSAQAHGHSVLQVAAHYRAAFFRDTALLGIEEPSIVCNASDCIQDMIAFIEQLLARGHAYCAGGNVYFDVRSFP
SYESFGSAAVEDVQEGEDAARARVAHDTHKRDARDFVLWFTRSKFVRHALTWDSPWGRGYPGWHIGCSAMSMKFLGPRCD
IHIGGVDHIRVHHRNERAQCEAITGAPWVRYWLHHEFLLMQLQKRAVHADMGSSVVSSFSKMSKSCGQFLTLSSLQERGF
QPADFRFFLLSGQYRTQLAFSWDALKTARAARRSFVRRVARVVDAARATTGSVRGTSAECAAERVCESRASESELLLTDF
RAALEDDFSTPRALSALQKLVRDTSVPPSLCVSALQVADTVLGLGIIQEATASLSAQVPAGDTLPQRPLPSEEWIGQLVR
ARAHARQTRDFPRADEIRRQLKAEGIELEDTHLGTIWKRV

Sequences:

>Translated_520_residues
MALRVYNTLTRQQEHFQPWEHGHVRLYGCGPTVYNYPHLGNLRAYVFQDTVRRTLHFLGYRVTYVMNITDVGHLESDADS
GEDKLVRSAQAHGHSVLQVAAHYRAAFFRDTALLGIEEPSIVCNASDCIQDMIAFIEQLLARGHAYCAGGNVYFDVRSFP
SYESFGSAAVEDVQEGEDAARARVAHDTHKRDARDFVLWFTRSKFVRHALTWDSPWGRGYPGWHIGCSAMSMKFLGPRCD
IHIGGVDHIRVHHRNERAQCEAITGAPWVRYWLHHEFLLMQLQKRAVHADMGSSVVSSFSKMSKSCGQFLTLSSLQERGF
QPADFRFFLLSGQYRTQLAFSWDALKTARAARRSFVRRVARVVDAARATTGSVRGTSAECAAERVCESRASESELLLTDF
RAALEDDFSTPRALSALQKLVRDTSVPPSLCVSALQVADTVLGLGIIQEATASLSAQVPAGDTLPQRPLPSEEWIGQLVR
ARAHARQTRDFPRADEIRRQLKAEGIELEDTHLGTIWKRV
>Mature_519_residues
ALRVYNTLTRQQEHFQPWEHGHVRLYGCGPTVYNYPHLGNLRAYVFQDTVRRTLHFLGYRVTYVMNITDVGHLESDADSG
EDKLVRSAQAHGHSVLQVAAHYRAAFFRDTALLGIEEPSIVCNASDCIQDMIAFIEQLLARGHAYCAGGNVYFDVRSFPS
YESFGSAAVEDVQEGEDAARARVAHDTHKRDARDFVLWFTRSKFVRHALTWDSPWGRGYPGWHIGCSAMSMKFLGPRCDI
HIGGVDHIRVHHRNERAQCEAITGAPWVRYWLHHEFLLMQLQKRAVHADMGSSVVSSFSKMSKSCGQFLTLSSLQERGFQ
PADFRFFLLSGQYRTQLAFSWDALKTARAARRSFVRRVARVVDAARATTGSVRGTSAECAAERVCESRASESELLLTDFR
AALEDDFSTPRALSALQKLVRDTSVPPSLCVSALQVADTVLGLGIIQEATASLSAQVPAGDTLPQRPLPSEEWIGQLVRA
RAHARQTRDFPRADEIRRQLKAEGIELEDTHLGTIWKRV

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=426, Percent_Identity=33.8028169014084, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI10835051, Length=280, Percent_Identity=30, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI21269877, Length=280, Percent_Identity=30, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI303304987, Length=280, Percent_Identity=30, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI62240992, Length=280, Percent_Identity=30, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1786737, Length=525, Percent_Identity=35.2380952380952, Blast_Score=263, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17509797, Length=271, Percent_Identity=30.6273062730627, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17509795, Length=271, Percent_Identity=30.6273062730627, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324082, Length=367, Percent_Identity=27.7929155313352, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19922218, Length=546, Percent_Identity=28.9377289377289, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI19922364, Length=355, Percent_Identity=29.5774647887324, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_TREPA (O83129)

Other databases:

- EMBL:   AE000520
- PIR:   D71368
- RefSeq:   NP_218531.1
- ProteinModelPortal:   O83129
- IntAct:   O83129
- MINT:   MINT-6479416
- GeneID:   2611268
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0091
- NMPDR:   fig|243276.1.peg.90
- TIGR:   TP_0091
- HOGENOM:   HBG327651
- OMA:   IGNLRTY
- ProtClustDB:   PRK14536
- BioCyc:   TPAL243276:TP_0091-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 58404; Mature: 58272

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRVYNTLTRQQEHFQPWEHGHVRLYGCGPTVYNYPHLGNLRAYVFQDTVRRTLHFLGY
CEEEHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RVTYVMNITDVGHLESDADSGEDKLVRSAQAHGHSVLQVAAHYRAAFFRDTALLGIEEPS
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHEEECCCCC
IVCNASDCIQDMIAFIEQLLARGHAYCAGGNVYFDVRSFPSYESFGSAAVEDVQEGEDAA
EEECHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHCCHHHH
RARVAHDTHKRDARDFVLWFTRSKFVRHALTWDSPWGRGYPGWHIGCSAMSMKFLGPRCD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHHCCCCCE
IHIGGVDHIRVHHRNERAQCEAITGAPWVRYWLHHEFLLMQLQKRAVHADMGSSVVSSFS
EEECCCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KMSKSCGQFLTLSSLQERGFQPADFRFFLLSGQYRTQLAFSWDALKTARAARRSFVRRVA
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
RVVDAARATTGSVRGTSAECAAERVCESRASESELLLTDFRAALEDDFSTPRALSALQKL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
VRDTSVPPSLCVSALQVADTVLGLGIIQEATASLSAQVPAGDTLPQRPLPSEEWIGQLVR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
ARAHARQTRDFPRADEIRRQLKAEGIELEDTHLGTIWKRV
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
ALRVYNTLTRQQEHFQPWEHGHVRLYGCGPTVYNYPHLGNLRAYVFQDTVRRTLHFLGY
EEEHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RVTYVMNITDVGHLESDADSGEDKLVRSAQAHGHSVLQVAAHYRAAFFRDTALLGIEEPS
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHEEECCCCC
IVCNASDCIQDMIAFIEQLLARGHAYCAGGNVYFDVRSFPSYESFGSAAVEDVQEGEDAA
EEECHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHCCHHHH
RARVAHDTHKRDARDFVLWFTRSKFVRHALTWDSPWGRGYPGWHIGCSAMSMKFLGPRCD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHHCCCCCE
IHIGGVDHIRVHHRNERAQCEAITGAPWVRYWLHHEFLLMQLQKRAVHADMGSSVVSSFS
EEECCCCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KMSKSCGQFLTLSSLQERGFQPADFRFFLLSGQYRTQLAFSWDALKTARAARRSFVRRVA
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
RVVDAARATTGSVRGTSAECAAERVCESRASESELLLTDFRAALEDDFSTPRALSALQKL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
VRDTSVPPSLCVSALQVADTVLGLGIIQEATASLSAQVPAGDTLPQRPLPSEEWIGQLVR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
ARAHARQTRDFPRADEIRRQLKAEGIELEDTHLGTIWKRV
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876