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The map label for this gene is dnaB

Identifier: 189025292

GI number: 189025292

Start: 67637

End: 68953

Strand: Reverse

Name: dnaB

Synonym: TPASS_0058

Alternate gene names: 189025292

Gene position: 68953-67637 (Counterclockwise)

Preceding gene: 189025293

Following gene: 189025287

Centisome position: 6.05

GC content: 53.0

Gene sequence:

>1317_bases
GTGCCGGGCATGCCTAATCCCACTCAGGAACTCAAGGGAAAAATTCCCCCTCATAACCTAGAGGCCGAGCGGGCTGTGCT
CGGCGCTGTTCTACTGGATGACTCTGCTCTGAGCACGGCAACAGAGCAGTTGTCTGCGAGCTCTTTTTATTCCGCTGCGC
ACCAGCGCATCTTTCAAGCACTCGTAGAGTTATCGGATTTAGGTCAACGCCCTGATATCCTCGTGCTCAGCGAGCACCTG
CGCTCCTGTGAGGCGCTCGATTTCGTCGGTGGCAGCGCGTATGTTGCCTCTCTCACCGATGCGGTCCCAAGCGCCGCGAA
TGTTGAATACTACACACGCATCGTTTGCGACGCTGCCATGCGCCGGTCGCTACTAAAAGTAGCCCGCATTATCACCGCAG
AGGCATTCAATGACACCGTGTCGGGCAACATCGTACTCGAAACAGCACAAAGGGAAATCTATGACCTAACAAATGCAAGG
AGAGTAGCAACCTTCAAATTGCTGAAAAACCTCATTCCTGATTTGGTCAATACTATTGAGACTCGTTACCGAAATCAAAG
CGATCTGGTCGGAATTGCCACCGGCCTAACGGCTCTGGATAATCTTACCGGTGGATTCCAGAACTCCGAGCTTATTGTCA
TAGGTGCGCGTCCTTCCATGGGGAAAACTGCGCTCGCCATGACTATGGCCTCGAACATTGCCATAAGGCAACGTATTCCA
ACCGCCTTTTTTTCTCTGGAAATGTCTAATTTGCTTCTGATGCAGCGACTTATCGCTGCAGAGTCAGGAGTATCCGCGAC
GAACCTGCGCAAAGGGCTTTTACAACTTTCCGATTTCGGGCGCATCCAAAACGCCGCAGGAGAGATGTACGACGCCCCAC
TTTACATCGTGGACGTGCCAAACATGAAGCTACTGGATCTCCGTGCCGTGGCTCGTCGATTATGCGTACAAGAAAAGATC
CAGATTATTTTTGTCGACTACTTGGGACTTATCGTAGCAGACAATCCCTTTGCGCCCCGCTACGAACAATTTGCAGCGAT
CTCCCAATCACTAAAAAGCCTCGCGCGCGAGCTAGACATCCCCATCGTAGCACTTTCGCAAGTCGGTCGACCGGCAGAGG
GCTCTGCGCCAAACCTGGCGGACATTCGTGGTTCGGGGGCAATTGAGCAGGACGCCGACGTGGTCATGTTTCTACACCGC
GACCGCAACGAAACCGAGACTCAACTCATTTTAGCGAAGCAACGCAACGGCCCCATTGGGACAGTTGAGCTCGAATTCCA
AGCGAGCTTTACCCGCTTCGTCTGCAAAAGCCCCTAG

Upstream 100 bases:

>100_bases
GGGCCTCAACCCTTTGCCTCGTTAGAGGGTTTCCACGGAGAGGGGGGGGACTGTCTTTTCACCACCTTCCCTCCTCTGAG
TATTCCTCGGAGGAGGTTCT

Downstream 100 bases:

>100_bases
CCACCTGAGAGGGTGTGCCCGCGCACGGAGGAAAGGGTACCTAAGCGGCAAAACCCCGCGGTTACTCTTCCTCCCTACCC
TCCGTAGGCCGAGATGAACA

Product: replicative DNA helicase DnaB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPGMPNPTQELKGKIPPHNLEAERAVLGAVLLDDSALSTATEQLSASSFYSAAHQRIFQALVELSDLGQRPDILVLSEHL
RSCEALDFVGGSAYVASLTDAVPSAANVEYYTRIVCDAAMRRSLLKVARIITAEAFNDTVSGNIVLETAQREIYDLTNAR
RVATFKLLKNLIPDLVNTIETRYRNQSDLVGIATGLTALDNLTGGFQNSELIVIGARPSMGKTALAMTMASNIAIRQRIP
TAFFSLEMSNLLLMQRLIAAESGVSATNLRKGLLQLSDFGRIQNAAGEMYDAPLYIVDVPNMKLLDLRAVARRLCVQEKI
QIIFVDYLGLIVADNPFAPRYEQFAAISQSLKSLARELDIPIVALSQVGRPAEGSAPNLADIRGSGAIEQDADVVMFLHR
DRNETETQLILAKQRNGPIGTVELEFQASFTRFVCKSP

Sequences:

>Translated_438_residues
MPGMPNPTQELKGKIPPHNLEAERAVLGAVLLDDSALSTATEQLSASSFYSAAHQRIFQALVELSDLGQRPDILVLSEHL
RSCEALDFVGGSAYVASLTDAVPSAANVEYYTRIVCDAAMRRSLLKVARIITAEAFNDTVSGNIVLETAQREIYDLTNAR
RVATFKLLKNLIPDLVNTIETRYRNQSDLVGIATGLTALDNLTGGFQNSELIVIGARPSMGKTALAMTMASNIAIRQRIP
TAFFSLEMSNLLLMQRLIAAESGVSATNLRKGLLQLSDFGRIQNAAGEMYDAPLYIVDVPNMKLLDLRAVARRLCVQEKI
QIIFVDYLGLIVADNPFAPRYEQFAAISQSLKSLARELDIPIVALSQVGRPAEGSAPNLADIRGSGAIEQDADVVMFLHR
DRNETETQLILAKQRNGPIGTVELEFQASFTRFVCKSP
>Mature_437_residues
PGMPNPTQELKGKIPPHNLEAERAVLGAVLLDDSALSTATEQLSASSFYSAAHQRIFQALVELSDLGQRPDILVLSEHLR
SCEALDFVGGSAYVASLTDAVPSAANVEYYTRIVCDAAMRRSLLKVARIITAEAFNDTVSGNIVLETAQREIYDLTNARR
VATFKLLKNLIPDLVNTIETRYRNQSDLVGIATGLTALDNLTGGFQNSELIVIGARPSMGKTALAMTMASNIAIRQRIPT
AFFSLEMSNLLLMQRLIAAESGVSATNLRKGLLQLSDFGRIQNAAGEMYDAPLYIVDVPNMKLLDLRAVARRLCVQEKIQ
IIFVDYLGLIVADNPFAPRYEQFAAISQSLKSLARELDIPIVALSQVGRPAEGSAPNLADIRGSGAIEQDADVVMFLHRD
RNETETQLILAKQRNGPIGTVELEFQASFTRFVCKSP

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain

Homologues:

Organism=Escherichia coli, GI1790486, Length=440, Percent_Identity=37.2727272727273, Blast_Score=268, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAB_TREPA (O83097)

Other databases:

- EMBL:   AE000520
- PIR:   B71370
- RefSeq:   NP_218498.1
- ProteinModelPortal:   O83097
- SMR:   O83097
- IntAct:   O83097
- GeneID:   2611409
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0058
- NMPDR:   fig|243276.1.peg.57
- TIGR:   TP_0058
- HOGENOM:   HBG734566
- OMA:   DINDADF
- ProtClustDB:   CLSK218497
- BioCyc:   TPAL243276:TP_0058-MONOMER
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- Gene3D:   G3DSA:1.10.860.10
- TIGRFAMs:   TIGR00665

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C; SSF48024 DNA_helicase_DnaB-like_N

EC number: =3.6.4.12

Molecular weight: Translated: 47797; Mature: 47665

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS51199 SF4_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGMPNPTQELKGKIPPHNLEAERAVLGAVLLDDSALSTATEQLSASSFYSAAHQRIFQA
CCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVELSDLGQRPDILVLSEHLRSCEALDFVGGSAYVASLTDAVPSAANVEYYTRIVCDAAM
HHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RRSLLKVARIITAEAFNDTVSGNIVLETAQREIYDLTNARRVATFKLLKNLIPDLVNTIE
HHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TRYRNQSDLVGIATGLTALDNLTGGFQNSELIVIGARPSMGKTALAMTMASNIAIRQRIP
HHHCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
TAFFSLEMSNLLLMQRLIAAESGVSATNLRKGLLQLSDFGRIQNAAGEMYDAPLYIVDVP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEECC
NMKLLDLRAVARRLCVQEKIQIIFVDYLGLIVADNPFAPRYEQFAAISQSLKSLARELDI
CCEEHHHHHHHHHHHHHHHHHEEEEEHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
PIVALSQVGRPAEGSAPNLADIRGSGAIEQDADVVMFLHRDRNETETQLILAKQRNGPIG
CEEEHHHCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCCHHEEEEEECCCCCEE
TVELEFQASFTRFVCKSP
EEEEEEECCHHHHHCCCC
>Mature Secondary Structure 
PGMPNPTQELKGKIPPHNLEAERAVLGAVLLDDSALSTATEQLSASSFYSAAHQRIFQA
CCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVELSDLGQRPDILVLSEHLRSCEALDFVGGSAYVASLTDAVPSAANVEYYTRIVCDAAM
HHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RRSLLKVARIITAEAFNDTVSGNIVLETAQREIYDLTNARRVATFKLLKNLIPDLVNTIE
HHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TRYRNQSDLVGIATGLTALDNLTGGFQNSELIVIGARPSMGKTALAMTMASNIAIRQRIP
HHHCCCCCEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
TAFFSLEMSNLLLMQRLIAAESGVSATNLRKGLLQLSDFGRIQNAAGEMYDAPLYIVDVP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEECC
NMKLLDLRAVARRLCVQEKIQIIFVDYLGLIVADNPFAPRYEQFAAISQSLKSLARELDI
CCEEHHHHHHHHHHHHHHHHHEEEEEHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
PIVALSQVGRPAEGSAPNLADIRGSGAIEQDADVVMFLHRDRNETETQLILAKQRNGPIG
CEEEHHHCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCCHHEEEEEECCCCCEE
TVELEFQASFTRFVCKSP
EEEEEEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876