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The map label for this gene is nrdF

Identifier: 189025287

GI number: 189025287

Start: 61735

End: 62790

Strand: Reverse

Name: nrdF

Synonym: TPASS_0053

Alternate gene names: 189025287

Gene position: 62790-61735 (Counterclockwise)

Preceding gene: 189025292

Following gene: 189025284

Centisome position: 5.51

GC content: 45.08

Gene sequence:

>1056_bases
ATGATGGAAAGTTCAACGATACTGCAAAGACGCGCGCTGTTTAATGAGGCAGGAGACATTGAACTTCATAAGCGACGCAT
GGTCGGGGGGAACACTACCAATCTCAACGACTTTAACAATATGAAGTATCCCTGGGTCAGCAAGTGGTATCGGCAGGCAA
TGAACAACTTCTGGATCCCCGAAGAAATCAACATGAGCAGTGACGTGCAAGATTATCGTAATCTATCCGCTATCGAGAAA
ACCGCGTACGATAAAATCCTCTCCTTCCTTATTTTCCTAGATAGCATCCAGACTGCGAATCTGCCAAATATTGGTCAGTA
CATTACCGCAAATGAGATTAACCTGTGCCTAACCATTCAGGCTTTCCAAGAGGCTGTGCATTCGCAGAGTTACAGTTACA
TGCTGGACACCATTTGCTCCCCCGAAGAACGCAATGACATTCTATACCAGTGGAAGGACGACGAGCATCTGCTTGCGCGC
AATAAATTCATTGGCAACTTGTACAACGAGTTTCAAGATGACAAAAGCGTACTCGCACTGCTGAAGGTAGCAATTGCGAA
TTATGTTTTGGAAGGAATTTATTTCTATTCGGGTTTTATGTTCTTCTATAACTTGGGGCGTAATAACAAGATGCCCGGCT
CGGTGCAGGAAATTCGTTATATCAACCGCGATGAAAACACACACCTGTGGCTTTTTCGATCCATTATTCAAGAACTGCAA
AAGGAAGAGCCACAGGTGTTCACTGCACGCAATGTACGCTTATTTCGCGATATGATTCGCGAAGGATGCGAACAAGAAAT
TAAGTGGGGGGACTATGTCATTGGGGATCAGATCCCTGGACTAAATCGCCACATGGTGGCAGACTATATCCGGTATTTGG
GTAATCTTCGTTGCGAAAATTTAGGCTTTGAGCCCTTGTACGAGGGGCACCGTGTAGAACCGGAATCGATGAGCTGGGTA
AGTCAGTACAGCAACGCAAACTTGATTAAAACCGATTTTTTTGAAGCAAAATCAACCGCGTACGCGAAGTCATCCGCAAT
GGTGGATGATCTCTAA

Upstream 100 bases:

>100_bases
CAGAGCCCTGCAGACAGACATTTTTACGCCCGCCGCAATTGAGAATTCAAAGTCCATCAAGTATACTACCCTGTGCCCGC
ACTCAGGAGGAGGTTTCATC

Downstream 100 bases:

>100_bases
CTCCTTCATGTACTCAGCCTATCGGCCAACGCGGCGCGGACATCCACCTGCAAACGCCAGGATAACCACTCAGTCGCCGT
GTTTCACCCTAGCCACTCGG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MMESSTILQRRALFNEAGDIELHKRRMVGGNTTNLNDFNNMKYPWVSKWYRQAMNNFWIPEEINMSSDVQDYRNLSAIEK
TAYDKILSFLIFLDSIQTANLPNIGQYITANEINLCLTIQAFQEAVHSQSYSYMLDTICSPEERNDILYQWKDDEHLLAR
NKFIGNLYNEFQDDKSVLALLKVAIANYVLEGIYFYSGFMFFYNLGRNNKMPGSVQEIRYINRDENTHLWLFRSIIQELQ
KEEPQVFTARNVRLFRDMIREGCEQEIKWGDYVIGDQIPGLNRHMVADYIRYLGNLRCENLGFEPLYEGHRVEPESMSWV
SQYSNANLIKTDFFEAKSTAYAKSSAMVDDL

Sequences:

>Translated_351_residues
MMESSTILQRRALFNEAGDIELHKRRMVGGNTTNLNDFNNMKYPWVSKWYRQAMNNFWIPEEINMSSDVQDYRNLSAIEK
TAYDKILSFLIFLDSIQTANLPNIGQYITANEINLCLTIQAFQEAVHSQSYSYMLDTICSPEERNDILYQWKDDEHLLAR
NKFIGNLYNEFQDDKSVLALLKVAIANYVLEGIYFYSGFMFFYNLGRNNKMPGSVQEIRYINRDENTHLWLFRSIIQELQ
KEEPQVFTARNVRLFRDMIREGCEQEIKWGDYVIGDQIPGLNRHMVADYIRYLGNLRCENLGFEPLYEGHRVEPESMSWV
SQYSNANLIKTDFFEAKSTAYAKSSAMVDDL
>Mature_351_residues
MMESSTILQRRALFNEAGDIELHKRRMVGGNTTNLNDFNNMKYPWVSKWYRQAMNNFWIPEEINMSSDVQDYRNLSAIEK
TAYDKILSFLIFLDSIQTANLPNIGQYITANEINLCLTIQAFQEAVHSQSYSYMLDTICSPEERNDILYQWKDDEHLLAR
NKFIGNLYNEFQDDKSVLALLKVAIANYVLEGIYFYSGFMFFYNLGRNNKMPGSVQEIRYINRDENTHLWLFRSIIQELQ
KEEPQVFTARNVRLFRDMIREGCEQEIKWGDYVIGDQIPGLNRHMVADYIRYLGNLRCENLGFEPLYEGHRVEPESMSWV
SQYSNANLIKTDFFEAKSTAYAKSSAMVDDL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family

Homologues:

Organism=Homo sapiens, GI42544136, Length=314, Percent_Identity=24.8407643312102, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI289177074, Length=314, Percent_Identity=24.8407643312102, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4557845, Length=304, Percent_Identity=24.3421052631579, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260064013, Length=304, Percent_Identity=24.3421052631579, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788567, Length=352, Percent_Identity=24.4318181818182, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789031, Length=303, Percent_Identity=26.4026402640264, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17551968, Length=311, Percent_Identity=23.1511254019293, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193206379, Length=265, Percent_Identity=23.0188679245283, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322434, Length=316, Percent_Identity=24.0506329113924, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18079279, Length=321, Percent_Identity=24.2990654205607, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIR2_TREPA (O83092)

Other databases:

- EMBL:   AE000520
- PIR:   G71372
- RefSeq:   NP_218493.1
- ProteinModelPortal:   O83092
- SMR:   O83092
- IntAct:   O83092
- MINT:   MINT-6480942
- GeneID:   2611427
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0053
- NMPDR:   fig|243276.1.peg.52
- TIGR:   TP_0053
- HOGENOM:   HBG321133
- OMA:   TLCSTPD
- ProtClustDB:   PRK09614
- BioCyc:   TPAL243276:TP_0053-MONOMER
- BRENDA:   1.17.4.1
- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358
- Gene3D:   G3DSA:1.10.620.20
- PANTHER:   PTHR23409

Pfam domain/function: PF00268 Ribonuc_red_sm; SSF47240 Ferritin/RR_like

EC number: =1.17.4.1

Molecular weight: Translated: 41199; Mature: 41199

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00368 RIBORED_SMALL

Important sites: ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMESSTILQRRALFNEAGDIELHKRRMVGGNTTNLNDFNNMKYPWVSKWYRQAMNNFWIP
CCCHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
EEINMSSDVQDYRNLSAIEKTAYDKILSFLIFLDSIQTANLPNIGQYITANEINLCLTIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEHH
AFQEAVHSQSYSYMLDTICSPEERNDILYQWKDDEHLLARNKFIGNLYNEFQDDKSVLAL
HHHHHHHHCHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LKVAIANYVLEGIYFYSGFMFFYNLGRNNKMPGSVQEIRYINRDENTHLWLFRSIIQELQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
KEEPQVFTARNVRLFRDMIREGCEQEIKWGDYVIGDQIPGLNRHMVADYIRYLGNLRCEN
HCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCC
LGFEPLYEGHRVEPESMSWVSQYSNANLIKTDFFEAKSTAYAKSSAMVDDL
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMESSTILQRRALFNEAGDIELHKRRMVGGNTTNLNDFNNMKYPWVSKWYRQAMNNFWIP
CCCHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
EEINMSSDVQDYRNLSAIEKTAYDKILSFLIFLDSIQTANLPNIGQYITANEINLCLTIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEHH
AFQEAVHSQSYSYMLDTICSPEERNDILYQWKDDEHLLARNKFIGNLYNEFQDDKSVLAL
HHHHHHHHCHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LKVAIANYVLEGIYFYSGFMFFYNLGRNNKMPGSVQEIRYINRDENTHLWLFRSIIQELQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
KEEPQVFTARNVRLFRDMIREGCEQEIKWGDYVIGDQIPGLNRHMVADYIRYLGNLRCEN
HCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCC
LGFEPLYEGHRVEPESMSWVSQYSNANLIKTDFFEAKSTAYAKSSAMVDDL
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876