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The map label for this gene is groEL

Identifier: 189025264

GI number: 189025264

Start: 38304

End: 39938

Strand: Direct

Name: groEL

Synonym: TPASS_0030

Alternate gene names: 189025264

Gene position: 38304-39938 (Clockwise)

Preceding gene: 189025263

Following gene: 189025265

Centisome position: 3.36

GC content: 50.15

Gene sequence:

>1635_bases
ATGGCGAAGCAATTGCTGTTTAATGAGGAAGCCCGCAAGAAGCTGCTTTCCGGGGTTGAGCAGATTTCGAGTGCGGTGAA
GGTAACGCTTGGTCCTAAGGGTCGCAATGTCTTGCTTGAAAAAGGGTACGGGGCTCCCACAGTCACGAAGGATGGGGTTT
CCGTTGCGAAAGAGGTTGAGCTCGAAGATCCGTTCGAGAATATGGGTGCACAGCTTTTAAAAGAGGTGGCTACGAAGACG
AACGACGTAGCTGGGGATGGCACAACTACTGCGACGGTATTGGCGTATTCGATGGTGCGTGAGGGTCTGAAGGCGGTTGC
TGCCGGTATGACGCCCCTTGAGTTGAAGCGTGGTATGGATAAGGCAGTTGCGATTGCAGTCGATGACATTAAGCAAAATT
CCAAGGGTATAAAGAGCAATGAAGAAGTCGCTCATGTAGCGTCAGTATCTGCGAATAACGACAAAGAGATTGGAAGGATT
CTGGCAAGCGCAATCGAGAAGGTGGGGAATGACGGGGTCATTGACGTTGACGAAGCCCAGACAATGGAAACGGTGACGGA
ATTCGTTGAAGGGATGCAGTTTGATCGTGGGTACATCTCGTCCTACTTCGTCACTGACCGAGATAGGATGGAAACGGTGT
ATGAAAATCCTTACATCCTTATCTACGATAAGTCCATCTCGACTATGAAGGATTTGCTTCCGCTACTCGAGAAAATTGCG
CAAACAGGTCGCCCGCTGCTCATCATAGCTGAGGATGTCGAAGGCGAAGCACTGGCTACGCTGGTGGTGAATAGTCTTCG
GGGAACGCTGAAGACGTGTGCGGTGAAGGCTCCCGGTTTCGGCGATAGGCGTAAAGAAATGCTTGAGGATATCGCGATTC
TTTCGGGCGGTCAGGTCATTTCCGAGGATTTAGGATTGAAGCTAGAGTCCGCGGATATTGCGCTCTTAGGTCAGGCAAAG
AGCGTGAAGGTAGACAAGGAGAATACCACGATTATTGACGGGTCGGGTAAGTCGAAGGATATCAAGGATCGTATAGAGCA
GATTAAAAAGCAAATAGAGGCCTCGACTTCAGATTATGACAGTGAGAAGTTGAAGGAGCGCTTGGCAAAGCTCTCTGGTG
GCGTTGCAGTTATCAAAATTGGTGCAGTCACCGAAGTGGAAATGAAAGAGAAGAAGCACCGGGTTGAAGATGCCTTAAAT
GCGACACGTGCGGCAATAGAGGAAGGTATTGTTGCTGGTGGTGGTTTAGCGCTTATTCAGGCTGCGGCGGCGCTCGAGAA
AGCTGATTTGAGTGGACTGACTCCAGATGAGGCGGTTGGTTTTAAGATTGTGCGTCGTGCTCTCGAGGAGCCGATACGCC
AGATTTCAGAGAACGCGGGTATTGATGGCGCAGTTGTGGCAGAGAAGGCAAAGGAGAAACGTGGCATCGGTTTTGATGCA
TCCAAGATGGAATGGGTTGATATGATTAAGGTCGGGATTATCGATCCGGCGAAGGTTACACGTTCAGCACTACAGAACGC
GGCTTCGGTTTCTGGGCTTTTGTTGACTACCGAATGTGCAATTGCTGCAATTCCCGAAAAGAGTTCCTCTACACCGCCAG
CTCCTGACATGGGAGGTATGGGAGGTATGTATTGA

Upstream 100 bases:

>100_bases
TGATGAAAAGATGTGAAGTGTTGCCAAGAATCTTTTTTTTTAGTACCCTCGGGGACCGTTACATATGAGGTGTTAACCTT
ACATTTAAGGAGTGAGAAGC

Downstream 100 bases:

>100_bases
TCTAGTTAGCGCCGGTCGATTGGTCATACGTAAGGGCATGATCTCTTGAGATGAGCGTCTCCCGTGGATCTCGGCCAGCG
TGTGGTGCGGGTAATTCCCC

Product: chaperonin GroEL

Products: NA

Alternate protein names: Common antigen; CA; GroEL protein; Protein Cpn60; TP4 antigen; TP-4; TPN60

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MAKQLLFNEEARKKLLSGVEQISSAVKVTLGPKGRNVLLEKGYGAPTVTKDGVSVAKEVELEDPFENMGAQLLKEVATKT
NDVAGDGTTTATVLAYSMVREGLKAVAAGMTPLELKRGMDKAVAIAVDDIKQNSKGIKSNEEVAHVASVSANNDKEIGRI
LASAIEKVGNDGVIDVDEAQTMETVTEFVEGMQFDRGYISSYFVTDRDRMETVYENPYILIYDKSISTMKDLLPLLEKIA
QTGRPLLIIAEDVEGEALATLVVNSLRGTLKTCAVKAPGFGDRRKEMLEDIAILSGGQVISEDLGLKLESADIALLGQAK
SVKVDKENTTIIDGSGKSKDIKDRIEQIKKQIEASTSDYDSEKLKERLAKLSGGVAVIKIGAVTEVEMKEKKHRVEDALN
ATRAAIEEGIVAGGGLALIQAAAALEKADLSGLTPDEAVGFKIVRRALEEPIRQISENAGIDGAVVAEKAKEKRGIGFDA
SKMEWVDMIKVGIIDPAKVTRSALQNAASVSGLLLTTECAIAAIPEKSSSTPPAPDMGGMGGMY

Sequences:

>Translated_544_residues
MAKQLLFNEEARKKLLSGVEQISSAVKVTLGPKGRNVLLEKGYGAPTVTKDGVSVAKEVELEDPFENMGAQLLKEVATKT
NDVAGDGTTTATVLAYSMVREGLKAVAAGMTPLELKRGMDKAVAIAVDDIKQNSKGIKSNEEVAHVASVSANNDKEIGRI
LASAIEKVGNDGVIDVDEAQTMETVTEFVEGMQFDRGYISSYFVTDRDRMETVYENPYILIYDKSISTMKDLLPLLEKIA
QTGRPLLIIAEDVEGEALATLVVNSLRGTLKTCAVKAPGFGDRRKEMLEDIAILSGGQVISEDLGLKLESADIALLGQAK
SVKVDKENTTIIDGSGKSKDIKDRIEQIKKQIEASTSDYDSEKLKERLAKLSGGVAVIKIGAVTEVEMKEKKHRVEDALN
ATRAAIEEGIVAGGGLALIQAAAALEKADLSGLTPDEAVGFKIVRRALEEPIRQISENAGIDGAVVAEKAKEKRGIGFDA
SKMEWVDMIKVGIIDPAKVTRSALQNAASVSGLLLTTECAIAAIPEKSSSTPPAPDMGGMGGMY
>Mature_543_residues
AKQLLFNEEARKKLLSGVEQISSAVKVTLGPKGRNVLLEKGYGAPTVTKDGVSVAKEVELEDPFENMGAQLLKEVATKTN
DVAGDGTTTATVLAYSMVREGLKAVAAGMTPLELKRGMDKAVAIAVDDIKQNSKGIKSNEEVAHVASVSANNDKEIGRIL
ASAIEKVGNDGVIDVDEAQTMETVTEFVEGMQFDRGYISSYFVTDRDRMETVYENPYILIYDKSISTMKDLLPLLEKIAQ
TGRPLLIIAEDVEGEALATLVVNSLRGTLKTCAVKAPGFGDRRKEMLEDIAILSGGQVISEDLGLKLESADIALLGQAKS
VKVDKENTTIIDGSGKSKDIKDRIEQIKKQIEASTSDYDSEKLKERLAKLSGGVAVIKIGAVTEVEMKEKKHRVEDALNA
TRAAIEEGIVAGGGLALIQAAAALEKADLSGLTPDEAVGFKIVRRALEEPIRQISENAGIDGAVVAEKAKEKRGIGFDAS
KMEWVDMIKVGIIDPAKVTRSALQNAASVSGLLLTTECAIAAIPEKSSSTPPAPDMGGMGGMY

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=46.8926553672316, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=46.8926553672316, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI5453607, Length=546, Percent_Identity=22.1611721611722, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=58.4905660377358, Blast_Score=616, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI17555558, Length=531, Percent_Identity=46.3276836158192, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI193210679, Length=213, Percent_Identity=47.887323943662, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=52.8625954198473, Blast_Score=534, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6322524, Length=514, Percent_Identity=23.3463035019455, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322350, Length=556, Percent_Identity=21.5827338129496, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=47.7272727272727, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=47.7272727272727, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI45550936, Length=526, Percent_Identity=45.6273764258555, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI45550132, Length=526, Percent_Identity=45.6273764258555, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI45550935, Length=526, Percent_Identity=45.6273764258555, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864606, Length=523, Percent_Identity=40.9177820267686, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24584129, Length=536, Percent_Identity=35.0746268656716, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI19921262, Length=536, Percent_Identity=35.0746268656716, Blast_Score=291, Evalue=6e-79,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_TREPA (P23033)

Other databases:

- EMBL:   X54111
- EMBL:   AE000520
- PIR:   H71374
- RefSeq:   NP_218470.1
- ProteinModelPortal:   P23033
- SMR:   P23033
- IntAct:   P23033
- MINT:   MINT-6478938
- GeneID:   2611151
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0030
- NMPDR:   fig|243276.1.peg.29
- TIGR:   TP_0030
- HOGENOM:   HBG625289
- OMA:   ATDKYED
- ProtClustDB:   PRK00013
- BioCyc:   TPAL243276:TP_0030-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57981; Mature: 57850

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQLLFNEEARKKLLSGVEQISSAVKVTLGPKGRNVLLEKGYGAPTVTKDGVSVAKEVE
CCCHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHCC
LEDPFENMGAQLLKEVATKTNDVAGDGTTTATVLAYSMVREGLKAVAAGMTPLELKRGMD
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
KAVAIAVDDIKQNSKGIKSNEEVAHVASVSANNDKEIGRILASAIEKVGNDGVIDVDEAQ
CEEEEEHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHH
TMETVTEFVEGMQFDRGYISSYFVTDRDRMETVYENPYILIYDKSISTMKDLLPLLEKIA
HHHHHHHHHHCCCCCCCHHHHEEECCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHH
QTGRPLLIIAEDVEGEALATLVVNSLRGTLKTCAVKAPGFGDRRKEMLEDIAILSGGQVI
HCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCHH
SEDLGLKLESADIALLGQAKSVKVDKENTTIIDGSGKSKDIKDRIEQIKKQIEASTSDYD
HHHHCCEEECCCEEEECCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
SEKLKERLAKLSGGVAVIKIGAVTEVEMKEKKHRVEDALNATRAAIEEGIVAGGGLALIQ
HHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AAAALEKADLSGLTPDEAVGFKIVRRALEEPIRQISENAGIDGAVVAEKAKEKRGIGFDA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
SKMEWVDMIKVGIIDPAKVTRSALQNAASVSGLLLTTECAIAAIPEKSSSTPPAPDMGGM
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECHHHEECCCCCCCCCCCCCCCCC
GGMY
CCCC
>Mature Secondary Structure 
AKQLLFNEEARKKLLSGVEQISSAVKVTLGPKGRNVLLEKGYGAPTVTKDGVSVAKEVE
CCHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHCC
LEDPFENMGAQLLKEVATKTNDVAGDGTTTATVLAYSMVREGLKAVAAGMTPLELKRGMD
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCC
KAVAIAVDDIKQNSKGIKSNEEVAHVASVSANNDKEIGRILASAIEKVGNDGVIDVDEAQ
CEEEEEHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHH
TMETVTEFVEGMQFDRGYISSYFVTDRDRMETVYENPYILIYDKSISTMKDLLPLLEKIA
HHHHHHHHHHCCCCCCCHHHHEEECCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHH
QTGRPLLIIAEDVEGEALATLVVNSLRGTLKTCAVKAPGFGDRRKEMLEDIAILSGGQVI
HCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCHH
SEDLGLKLESADIALLGQAKSVKVDKENTTIIDGSGKSKDIKDRIEQIKKQIEASTSDYD
HHHHCCEEECCCEEEECCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
SEKLKERLAKLSGGVAVIKIGAVTEVEMKEKKHRVEDALNATRAAIEEGIVAGGGLALIQ
HHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AAAALEKADLSGLTPDEAVGFKIVRRALEEPIRQISENAGIDGAVVAEKAKEKRGIGFDA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
SKMEWVDMIKVGIIDPAKVTRSALQNAASVSGLLLTTECAIAAIPEKSSSTPPAPDMGGM
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECHHHEECCCCCCCCCCCCCCCCC
GGMY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876; 1971618