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The map label for this gene is mnmG

Identifier: 188587518

GI number: 188587518

Start: 3159517

End: 3161451

Strand: Reverse

Name: mnmG

Synonym: Nther_2929

Alternate gene names: 188587518

Gene position: 3161451-3159517 (Counterclockwise)

Preceding gene: 188587519

Following gene: 188587517

Centisome position: 99.87

GC content: 35.87

Gene sequence:

>1935_bases
ATGGCCTATTATGGTGGCAATTATGATTTAATTGTAGTGGGCGCAGGACATGCTGGCTGTGAAGCCGCTTTAGCTGGTGC
TAGAATTGGGGCTAAAACTTTGTTATTGACTTTAAATTTAGACCAAATAGCTCTAATGCCATGTAATCCTGCTATTGGAG
GTCCGGCAAAAAGTCACATTGTCAGAGAAGTTGATGCTTTAGGGGGAGAAATGGGCAAAAATATAGATGATACTGCTATT
CAATTTAGGATGTTAAATACCCGCAAAGGTCCAGCTGTTCAGGCTTTGAGGGCTCAAGCTGATAAAATTAAATATCAAGA
ACGAATGAAATACATTTTAGAAAATGAAGAAAATCTCCTTCTCAAACAAGAAACAGTTAATCAGTTAATGATAGAAGACA
ATCAAATTAAAGGTGTCGTTACTAAAGATGGCACTTATTATTATTGTGAACAACTCGTGATTACCTCAGGAACTTATTTA
AAGGGAAGAATTATAGTAGGGGATGTAGACTACTCTGGAGGTCCTAATGGCCAGCAAGCTGCCTATGGTTTATCAGAGGA
CTTTAAACAGCACGGAATTTCTTTAATGAGATTTAAAACAGGGACTCCTGCTAGAGTGTATAGAAAAAGTTTGAATTTCG
ATGAAATGGAGATTCAGCATGGGGATGATTATCCCTGGAGATTTTCTTTTTATCATAGTTCTGAAAGACTGAAACAATTG
CCATGTTATTTAACTTTCACTAATTATGAAACCCATAAAATAATTAATGATAGTTTAAGTAGATCTCCTCTTTATTCTGG
AAGAATAGAAGGTACCGGTCCAAGATATTGCCCCTCTATTGAAGATAAAATTGTTAGATTTCCTGACAAATCACGACATC
AACTATTTTTAGAACCTGAAGGATTAAATACTCAGGAATACTATGTTCAGGGAATGTCAACAAGCTTACCATTAGATGTT
CAGGAAAGGATGCTCAGAACTATACCTGGTTTAGAAGAAGTTGAAATAATGAGACCTGGATATGCTATAGAATATGATTG
TATTGACCCAACCCAGTTAGAATTAACTCTAGAACACAAACAAATATCTGGATTGTTTTTTGCTGGTCAAATCAACGGTA
CTAGTGGGTATGAGGAAGCTGCTGGTCAAGGAATTTTAGCAGGAATAAATGCAGCTCAAAAAATTCAAGGTAAAGAGCCT
GTTATTATCAAACGTAGTCAAGGTTATATAGGAGTATTAATTGATGATCTTGTTACAAAAGGGACTCAAGAGCCATATAG
GATGTTAACTTCCCGCGCCGAATATCGTTTGATACTGAGACAAGATAATGCAGATTTAAGGCTAACCGAACTTGGTTATC
AAGTAGGGCTTATTAAAGAAGGCAATTATCAAAAATTCAAAGAGAAACTAGAGAAGATAGAAAGCGAAAAAGAGAGGCTT
AAATCAATTAAGTTAACACCTAATAAAAATGTACAAAACTTTATGGAAGAACACAATACCTCTGGGTTAAAAAAACCTAC
AACCTTATTTCACATCTTAAAAAGGCCAGAAATTTCATATGAAGCACTGGAGTATTTCGATGAAACAAGACCTGATTTAC
CTTTAGAAGTTACAGAACAAATTGAAATACAAATTAAGTATGAGGGATATATCGATAAACAAGTGGAACAGGTGCAAAGG
TTTGAAAAGTTGGAAGCTCGTTTAATTCCTGATGATATTGATTATAGTCAAATTTCAGGTTTGTCAATAGAAGCAAGGGA
AAAATTGCAAACGTACAGACCCAGATCCGTAGGGCAAGCAAATCGGATTTCTGGAATAGACCCTTCAGATATTTCTGTGT
TGATGGTTTATCTTGAACAACTGAAACAGCGTGCGGACAGGAATCAGGCCAGTCATGATGATTCAAATACAGAGACAGGT
GAAAATGATGACTAA

Upstream 100 bases:

>100_bases
GCTTATGATTACTTAGGTGAAATCACAGGTGAAACTGCTTCAGAAGAACTTGTAGATCGAATTTTTAATGATTTTTGTAT
TGGAAAGTGAGGTTGTTAAT

Downstream 100 bases:

>100_bases
TTTATCTAACTTATCTGACAAGATTAAGCAGTGGGGAGAAACAGCTCCAAAGGAAATAGATGAAAGTTTTATTGTATATA
AAGAAGAGTTACTGGACTGG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MAYYGGNYDLIVVGAGHAGCEAALAGARIGAKTLLLTLNLDQIALMPCNPAIGGPAKSHIVREVDALGGEMGKNIDDTAI
QFRMLNTRKGPAVQALRAQADKIKYQERMKYILENEENLLLKQETVNQLMIEDNQIKGVVTKDGTYYYCEQLVITSGTYL
KGRIIVGDVDYSGGPNGQQAAYGLSEDFKQHGISLMRFKTGTPARVYRKSLNFDEMEIQHGDDYPWRFSFYHSSERLKQL
PCYLTFTNYETHKIINDSLSRSPLYSGRIEGTGPRYCPSIEDKIVRFPDKSRHQLFLEPEGLNTQEYYVQGMSTSLPLDV
QERMLRTIPGLEEVEIMRPGYAIEYDCIDPTQLELTLEHKQISGLFFAGQINGTSGYEEAAGQGILAGINAAQKIQGKEP
VIIKRSQGYIGVLIDDLVTKGTQEPYRMLTSRAEYRLILRQDNADLRLTELGYQVGLIKEGNYQKFKEKLEKIESEKERL
KSIKLTPNKNVQNFMEEHNTSGLKKPTTLFHILKRPEISYEALEYFDETRPDLPLEVTEQIEIQIKYEGYIDKQVEQVQR
FEKLEARLIPDDIDYSQISGLSIEAREKLQTYRPRSVGQANRISGIDPSDISVLMVYLEQLKQRADRNQASHDDSNTETG
ENDD

Sequences:

>Translated_644_residues
MAYYGGNYDLIVVGAGHAGCEAALAGARIGAKTLLLTLNLDQIALMPCNPAIGGPAKSHIVREVDALGGEMGKNIDDTAI
QFRMLNTRKGPAVQALRAQADKIKYQERMKYILENEENLLLKQETVNQLMIEDNQIKGVVTKDGTYYYCEQLVITSGTYL
KGRIIVGDVDYSGGPNGQQAAYGLSEDFKQHGISLMRFKTGTPARVYRKSLNFDEMEIQHGDDYPWRFSFYHSSERLKQL
PCYLTFTNYETHKIINDSLSRSPLYSGRIEGTGPRYCPSIEDKIVRFPDKSRHQLFLEPEGLNTQEYYVQGMSTSLPLDV
QERMLRTIPGLEEVEIMRPGYAIEYDCIDPTQLELTLEHKQISGLFFAGQINGTSGYEEAAGQGILAGINAAQKIQGKEP
VIIKRSQGYIGVLIDDLVTKGTQEPYRMLTSRAEYRLILRQDNADLRLTELGYQVGLIKEGNYQKFKEKLEKIESEKERL
KSIKLTPNKNVQNFMEEHNTSGLKKPTTLFHILKRPEISYEALEYFDETRPDLPLEVTEQIEIQIKYEGYIDKQVEQVQR
FEKLEARLIPDDIDYSQISGLSIEAREKLQTYRPRSVGQANRISGIDPSDISVLMVYLEQLKQRADRNQASHDDSNTETG
ENDD
>Mature_643_residues
AYYGGNYDLIVVGAGHAGCEAALAGARIGAKTLLLTLNLDQIALMPCNPAIGGPAKSHIVREVDALGGEMGKNIDDTAIQ
FRMLNTRKGPAVQALRAQADKIKYQERMKYILENEENLLLKQETVNQLMIEDNQIKGVVTKDGTYYYCEQLVITSGTYLK
GRIIVGDVDYSGGPNGQQAAYGLSEDFKQHGISLMRFKTGTPARVYRKSLNFDEMEIQHGDDYPWRFSFYHSSERLKQLP
CYLTFTNYETHKIINDSLSRSPLYSGRIEGTGPRYCPSIEDKIVRFPDKSRHQLFLEPEGLNTQEYYVQGMSTSLPLDVQ
ERMLRTIPGLEEVEIMRPGYAIEYDCIDPTQLELTLEHKQISGLFFAGQINGTSGYEEAAGQGILAGINAAQKIQGKEPV
IIKRSQGYIGVLIDDLVTKGTQEPYRMLTSRAEYRLILRQDNADLRLTELGYQVGLIKEGNYQKFKEKLEKIESEKERLK
SIKLTPNKNVQNFMEEHNTSGLKKPTTLFHILKRPEISYEALEYFDETRPDLPLEVTEQIEIQIKYEGYIDKQVEQVQRF
EKLEARLIPDDIDYSQISGLSIEAREKLQTYRPRSVGQANRISGIDPSDISVLMVYLEQLKQRADRNQASHDDSNTETGE
NDD

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=652, Percent_Identity=39.8773006134969, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI19882217, Length=677, Percent_Identity=38.4047267355982, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI183227703, Length=692, Percent_Identity=37.5722543352601, Blast_Score=442, Evalue=1e-124,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=50.6451612903226, Blast_Score=639, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=617, Percent_Identity=41.4910858995138, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321202, Length=614, Percent_Identity=43.6482084690554, Blast_Score=486, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24658174, Length=635, Percent_Identity=41.7322834645669, Blast_Score=441, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_NATTJ (B2A469)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001919063.1
- ProteinModelPortal:   B2A469
- SMR:   B2A469
- GeneID:   6317165
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2929
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 73031; Mature: 72900

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYYGGNYDLIVVGAGHAGCEAALAGARIGAKTLLLTLNLDQIALMPCNPAIGGPAKSHI
CCEECCCEEEEEEECCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHH
VREVDALGGEMGKNIDDTAIQFRMLNTRKGPAVQALRAQADKIKYQERMKYILENEENLL
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LKQETVNQLMIEDNQIKGVVTKDGTYYYCEQLVITSGTYLKGRIIVGDVDYSGGPNGQQA
EEHHHHHHHEECCCCEEEEEECCCCEEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCHH
AYGLSEDFKQHGISLMRFKTGTPARVYRKSLNFDEMEIQHGDDYPWRFSFYHSSERLKQL
HCCCHHHHHHCCCEEEEEECCCCHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHHHHHC
PCYLTFTNYETHKIINDSLSRSPLYSGRIEGTGPRYCPSIEDKIVRFPDKSRHQLFLEPE
CEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEECCC
GLNTQEYYVQGMSTSLPLDVQERMLRTIPGLEEVEIMRPGYAIEYDCIDPTQLELTLEHK
CCCHHHHHEECCCCCCCCCHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCEEEEEEECE
QISGLFFAGQINGTSGYEEAAGQGILAGINAAQKIQGKEPVIIKRSQGYIGVLIDDLVTK
EECEEEEEEEECCCCCHHHHCCCCEEECCCHHHHCCCCCCEEEEECCCEEEEEHHHHHHC
GTQEPYRMLTSRAEYRLILRQDNADLRLTELGYQVGLIKEGNYQKFKEKLEKIESEKERL
CCCHHHHHHHCCCCEEEEEEECCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHH
KSIKLTPNKNVQNFMEEHNTSGLKKPTTLFHILKRPEISYEALEYFDETRPDLPLEVTEQ
HEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECE
IEIQIKYEGYIDKQVEQVQRFEKLEARLIPDDIDYSQISGLSIEAREKLQTYRPRSVGQA
EEEEEEECCCCHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCC
NRISGIDPSDISVLMVYLEQLKQRADRNQASHDDSNTETGENDD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYYGGNYDLIVVGAGHAGCEAALAGARIGAKTLLLTLNLDQIALMPCNPAIGGPAKSHI
CEECCCEEEEEEECCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHH
VREVDALGGEMGKNIDDTAIQFRMLNTRKGPAVQALRAQADKIKYQERMKYILENEENLL
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LKQETVNQLMIEDNQIKGVVTKDGTYYYCEQLVITSGTYLKGRIIVGDVDYSGGPNGQQA
EEHHHHHHHEECCCCEEEEEECCCCEEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCHH
AYGLSEDFKQHGISLMRFKTGTPARVYRKSLNFDEMEIQHGDDYPWRFSFYHSSERLKQL
HCCCHHHHHHCCCEEEEEECCCCHHHHHHHCCCCEEEEECCCCCCEEEEEECCHHHHHHC
PCYLTFTNYETHKIINDSLSRSPLYSGRIEGTGPRYCPSIEDKIVRFPDKSRHQLFLEPE
CEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEECCC
GLNTQEYYVQGMSTSLPLDVQERMLRTIPGLEEVEIMRPGYAIEYDCIDPTQLELTLEHK
CCCHHHHHEECCCCCCCCCHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCEEEEEEECE
QISGLFFAGQINGTSGYEEAAGQGILAGINAAQKIQGKEPVIIKRSQGYIGVLIDDLVTK
EECEEEEEEEECCCCCHHHHCCCCEEECCCHHHHCCCCCCEEEEECCCEEEEEHHHHHHC
GTQEPYRMLTSRAEYRLILRQDNADLRLTELGYQVGLIKEGNYQKFKEKLEKIESEKERL
CCCHHHHHHHCCCCEEEEEEECCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHH
KSIKLTPNKNVQNFMEEHNTSGLKKPTTLFHILKRPEISYEALEYFDETRPDLPLEVTEQ
HEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECE
IEIQIKYEGYIDKQVEQVQRFEKLEARLIPDDIDYSQISGLSIEAREKLQTYRPRSVGQA
EEEEEEECCCCHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCC
NRISGIDPSDISVLMVYLEQLKQRADRNQASHDDSNTETGENDD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA