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The map label for this gene is mnmE

Identifier: 188587519

GI number: 188587519

Start: 3161462

End: 3162838

Strand: Reverse

Name: mnmE

Synonym: Nther_2930

Alternate gene names: 188587519

Gene position: 3162838-3161462 (Counterclockwise)

Preceding gene: 188587520

Following gene: 188587518

Centisome position: 99.91

GC content: 34.71

Gene sequence:

>1377_bases
TTGACTGAGGATACCATCGCAGCATTATCTACTCCTCCTGGAGAAGGTGGCATAGCAGTCATTAGAGTAAGTGGTCCAGA
TAGTCAAAATAAAGTGAAACAGATATTTAGAAGTTCACGAACTGATGGAAACTTTAATAATAAGAAGATGTATCATGGTC
AGATCGTATCGCCAGAAACCAATAGAATTTTAGATGAAGTTTTAGTTGTATTTATGAACAAGCCCTATACTTACACTTGT
GAAGACGTAGTTGAAATACATTGTCATGGTGGAATGGTACCAGTTAAAGAGATCTTACAATTATTGTTTAGTTACGGTAT
TAGACCAGCTGAACCTGGTGAGTTCACAAAACGAGCTTTTTTAAATGGTCGTTTGGATTTAACGCAGGCGGAAGGGGTTA
TGGACTTAATTACCTCTAAGACAAATAACTTAAAAAATGTTGCTATAAATCAACTACAAGGAAATCTTAAACAGAAAATA
GATAGATTAAGAGATGATTTAGTATCTGTAATGGCAAACTTAGAAGCGAGAATTGATTTCCCCGATGAAGATATTGATGT
AGAGGATTATCATGAATTAAAACATAGAATTGACAATGCCAAGGTTGACATAAACAATTTGATTGCAAGTTACGATAAAG
GCAAAATAATTAGAGAAGGAATCAAAACTGTTATTGTTGGGAGACCGAATGTAGGCAAATCTTCTTTATTAAATCTACTC
TTGGGAGAAGAACGTGCTATAGTCACAGAAATTCCTGGAACTACCAGGGATGTTTTAGAAGAAGTGATAAATTTAAAGGG
AATCCCTTTGCGCATAATAGATACTGCCGGAATTAGGGAATCTGAAGACAAAGTAGAACAAATTGGAGTTAAACGTACTA
GAGATTCTATGGAGCAAGCCGATATTATTCTGGTTGTAATAGATTCTTCTCAGGAATTAAGCCAGGAGGATAAACAAATC
TTGACAATGGCTCAGGATAAAACTTCTTTACTTGTATTAAATAAAACTGATCTCCATGAAAAACTTGATATAGATGAAAT
TGATAAATTAGTCTCCCAAATACCAAAAGTGCGGATTTCTGCGCTTAAAGAAGAGGGTCTGGATAAATTGGAAGAACATA
TTTCTGAATTGGTTTTTGGTGGGCAAGTTATGCAGACGGAGGAATTAGTAATTACAAAAGCTCGCCATTTTCATTCTCTT
GACAAAGTTAAGGAAGCTCTAAGTTCAGCAGAAGAAAATATCAAGGCTGAAATGAGTGAGGATTTAATTGCTATAGATAT
AAAAGAGGCTTATGATTACTTAGGTGAAATCACAGGTGAAACTGCTTCAGAAGAACTTGTAGATCGAATTTTTAATGATT
TTTGTATTGGAAAGTGA

Upstream 100 bases:

>100_bases
GTAGTGCAGATTCACTTAAACAAAAACCGCCTTTAGGGGCGGTTTTCTTATAACTTGGTTATTGCCAATAGACTATAAAT
AATAATAGAGGTGATATAGA

Downstream 100 bases:

>100_bases
GGTTGTTAATATGGCCTATTATGGTGGCAATTATGATTTAATTGTAGTGGGCGCAGGACATGCTGGCTGTGAAGCCGCTT
TAGCTGGTGCTAGAATTGGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTEDTIAALSTPPGEGGIAVIRVSGPDSQNKVKQIFRSSRTDGNFNNKKMYHGQIVSPETNRILDEVLVVFMNKPYTYTC
EDVVEIHCHGGMVPVKEILQLLFSYGIRPAEPGEFTKRAFLNGRLDLTQAEGVMDLITSKTNNLKNVAINQLQGNLKQKI
DRLRDDLVSVMANLEARIDFPDEDIDVEDYHELKHRIDNAKVDINNLIASYDKGKIIREGIKTVIVGRPNVGKSSLLNLL
LGEERAIVTEIPGTTRDVLEEVINLKGIPLRIIDTAGIRESEDKVEQIGVKRTRDSMEQADIILVVIDSSQELSQEDKQI
LTMAQDKTSLLVLNKTDLHEKLDIDEIDKLVSQIPKVRISALKEEGLDKLEEHISELVFGGQVMQTEELVITKARHFHSL
DKVKEALSSAEENIKAEMSEDLIAIDIKEAYDYLGEITGETASEELVDRIFNDFCIGK

Sequences:

>Translated_458_residues
MTEDTIAALSTPPGEGGIAVIRVSGPDSQNKVKQIFRSSRTDGNFNNKKMYHGQIVSPETNRILDEVLVVFMNKPYTYTC
EDVVEIHCHGGMVPVKEILQLLFSYGIRPAEPGEFTKRAFLNGRLDLTQAEGVMDLITSKTNNLKNVAINQLQGNLKQKI
DRLRDDLVSVMANLEARIDFPDEDIDVEDYHELKHRIDNAKVDINNLIASYDKGKIIREGIKTVIVGRPNVGKSSLLNLL
LGEERAIVTEIPGTTRDVLEEVINLKGIPLRIIDTAGIRESEDKVEQIGVKRTRDSMEQADIILVVIDSSQELSQEDKQI
LTMAQDKTSLLVLNKTDLHEKLDIDEIDKLVSQIPKVRISALKEEGLDKLEEHISELVFGGQVMQTEELVITKARHFHSL
DKVKEALSSAEENIKAEMSEDLIAIDIKEAYDYLGEITGETASEELVDRIFNDFCIGK
>Mature_457_residues
TEDTIAALSTPPGEGGIAVIRVSGPDSQNKVKQIFRSSRTDGNFNNKKMYHGQIVSPETNRILDEVLVVFMNKPYTYTCE
DVVEIHCHGGMVPVKEILQLLFSYGIRPAEPGEFTKRAFLNGRLDLTQAEGVMDLITSKTNNLKNVAINQLQGNLKQKID
RLRDDLVSVMANLEARIDFPDEDIDVEDYHELKHRIDNAKVDINNLIASYDKGKIIREGIKTVIVGRPNVGKSSLLNLLL
GEERAIVTEIPGTTRDVLEEVINLKGIPLRIIDTAGIRESEDKVEQIGVKRTRDSMEQADIILVVIDSSQELSQEDKQIL
TMAQDKTSLLVLNKTDLHEKLDIDEIDKLVSQIPKVRISALKEEGLDKLEEHISELVFGGQVMQTEELVITKARHFHSLD
KVKEALSSAEENIKAEMSEDLIAIDIKEAYDYLGEITGETASEELVDRIFNDFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=37.8723404255319, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=35.8565737051793, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=37.2549019607843, Blast_Score=249, Evalue=4e-66,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=36.5800865800866, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=40.9836065573771, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=461, Percent_Identity=30.8026030368764, Blast_Score=201, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6323665, Length=501, Percent_Identity=33.3333333333333, Blast_Score=227, Evalue=3e-60,
Organism=Drosophila melanogaster, GI45550813, Length=478, Percent_Identity=33.0543933054393, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_NATTJ (B2A470)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001919064.1
- GeneID:   6317166
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2930
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51302; Mature: 51171

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 85-85 BINDING 124-124 BINDING 458-458

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDTIAALSTPPGEGGIAVIRVSGPDSQNKVKQIFRSSRTDGNFNNKKMYHGQIVSPET
CCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEECCEEECCHH
NRILDEVLVVFMNKPYTYTCEDVVEIHCHGGMVPVKEILQLLFSYGIRPAEPGEFTKRAF
HHHHHHHHHHHHCCCCEEEHHHHHEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
LNGRLDLTQAEGVMDLITSKTNNLKNVAINQLQGNLKQKIDRLRDDLVSVMANLEARIDF
HCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECC
PDEDIDVEDYHELKHRIDNAKVDINNLIASYDKGKIIREGIKTVIVGRPNVGKSSLLNLL
CCCCCCHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHCCCEEEECCCCCCHHHHHHHH
LGEERAIVTEIPGTTRDVLEEVINLKGIPLRIIDTAGIRESEDKVEQIGVKRTRDSMEQA
HCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHC
DIILVVIDSSQELSQEDKQILTMAQDKTSLLVLNKTDLHEKLDIDEIDKLVSQIPKVRIS
CEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHHHHHHHHHCCHHHHH
ALKEEGLDKLEEHISELVFGGQVMQTEELVITKARHFHSLDKVKEALSSAEENIKAEMSE
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLIAIDIKEAYDYLGEITGETASEELVDRIFNDFCIGK
CEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEDTIAALSTPPGEGGIAVIRVSGPDSQNKVKQIFRSSRTDGNFNNKKMYHGQIVSPET
CCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEECCEEECCHH
NRILDEVLVVFMNKPYTYTCEDVVEIHCHGGMVPVKEILQLLFSYGIRPAEPGEFTKRAF
HHHHHHHHHHHHCCCCEEEHHHHHEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
LNGRLDLTQAEGVMDLITSKTNNLKNVAINQLQGNLKQKIDRLRDDLVSVMANLEARIDF
HCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECC
PDEDIDVEDYHELKHRIDNAKVDINNLIASYDKGKIIREGIKTVIVGRPNVGKSSLLNLL
CCCCCCHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHCCCEEEECCCCCCHHHHHHHH
LGEERAIVTEIPGTTRDVLEEVINLKGIPLRIIDTAGIRESEDKVEQIGVKRTRDSMEQA
HCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHC
DIILVVIDSSQELSQEDKQILTMAQDKTSLLVLNKTDLHEKLDIDEIDKLVSQIPKVRIS
CEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHHHHHHHHHCCHHHHH
ALKEEGLDKLEEHISELVFGGQVMQTEELVITKARHFHSLDKVKEALSSAEENIKAEMSE
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLIAIDIKEAYDYLGEITGETASEELVDRIFNDFCIGK
CEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA