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The map label for this gene is pyrG

Identifier: 188587477

GI number: 188587477

Start: 3118187

End: 3119800

Strand: Direct

Name: pyrG

Synonym: Nther_2887

Alternate gene names: 188587477

Gene position: 3118187-3119800 (Clockwise)

Preceding gene: 188587476

Following gene: 188587481

Centisome position: 98.5

GC content: 38.91

Gene sequence:

>1614_bases
ATGAGTAAATACATTTTCGTAACCGGAGGCGTAGCATCATCCCTGGGAAAAGGAATCTCAGCAGCTTCAATCGGTCGCTT
ACTAAAAAATCGTGGCTTAAATGTCACTATCCAAAAGTTTGATCCTTATATTAATATTGATCCTGGCACAATGAGCCCAT
ACCAACACGGTGAAGTTTTTGTGACCGAGGACGGTGCCGAAACCGACCTAGACCTGGGTCATTATGAGCGATTTATTGAT
GAAAACCTAAGTAGAATCAACAATATAACCACTGGGAAAATCTACTGGTCAGTACTCAGTAAAGAAAGAAAAGGTGAGTA
TTTAGGAAAAACTGTGCAAGTAATCCCCCATATAACCGATGAGATTAAATCTCGCATTTACCAGGCCAGTAAAGATAACG
ATGCAGATGTGGTAATAGCTGAAATAGGTGGAACTGTGGGGGATATTGAAAGTCTACCCTTTTTAGAGGCTATTAGGCAA
ATGAGAAGTGATATCGGTCGTGATTCCGTCATGTATATTCACGTTACCCTAGTCCCTTATTTGGAAAAATCCGGGGAATT
AAAAACTAAACCTACTCAACACAGTGTAAAAGAACTCAGAAGCCTAGGTATTCAACCCGATATGGTAATTTGTCGAACTC
ATTACGAACTGTCCCAAGAAATCAAAGAAAAACTAGCATTATTTTGTGATATTGATGTAAATGCAGTCATTGAAAATTTA
GATGTAGAAAATATTTATGAAGTACCTATGGCCCTGAATGAACAGCGGGTAGATGGACTAATAGCAGATAGGTTAAATTT
AAATTCAGGTAGCTTGGAGTTGGAAAGATGGAAGCAACTTGTTGACCAGATAAAGAATTTATCTCAGGAAATAACTATCG
GGTTAGTAGGAAAATACGTTTCTCTACAGGATGCCTATATTAGCGTAACAGAGGCTTTGGACCACGCTGGTTTTAATTAC
GATTCCAAAGTTAAAGTCAAATGGATTAACTCTGAGGAATTAGAGAAAAACGGTTCAGTTGAAACAGCATTTCAAGGAGT
TGACGGTATTTTAGTCCCAGGTGGTTTCGGAGATAGAGGAATTGGAGGAAAAATAATGGCAGCTCAATATGCCAGGAAAA
ACAATATTCCATTTTTAGGAATTTGTCTTGGTATGCATTGTTCTGTAATTGATTTTGCTCACGAAGTTGCTGGTTTAAAT
GGGGCTTACAGTTCGGAGTTTCATCCAGAAACACCATATCCTGTAATTGATCTGCTCCCCGAACAAAAGGAGGTAAAAGA
TCTAGGGGGAACTATGCGTCTAGGTGCTTATCCATGCAAGTTAATTGAGGGAACTCGTGCCTATGAAGCCTATCAACAGG
ATATCGTCCAGGAACGACATAGGCATAGATACGAATTTAATAATGATTACAAAGAACAGCTTACGAACAATGGCCTAGTT
GTCTCAGGGGTTTCACCTGATGATCGTCTTGTTGAAATTGTGGAGTTAGAAGATCATCCATGGTTTGTAGCCACTCAATT
CCATCCAGAATTTAAATCAAGACCAGGAGCTCCACATCCTTTATTTAATAGTTTTGTTCAGCAAGCATTAAAAAACAGCC
TTTCATCCAAATGA

Upstream 100 bases:

>100_bases
ATTTAAAATCATAATTTAACCGGTTGCAAATCCAGTGTTAGTATTAGATAATATAAGACATGAGTGCAAGTCGTGGAATA
CTATACAGGAGGGTGCCAGA

Downstream 100 bases:

>100_bases
TGAGAGGCTGTTTTTATTAACACTCATTTATGCTTTGTTGATTATTACAGATTATAGCTACAAAATCCTGTAAAATTTTA
AACCTAACCTGTTCTCCAAT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSKYIFVTGGVASSLGKGISAASIGRLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFID
ENLSRINNITTGKIYWSVLSKERKGEYLGKTVQVIPHITDEIKSRIYQASKDNDADVVIAEIGGTVGDIESLPFLEAIRQ
MRSDIGRDSVMYIHVTLVPYLEKSGELKTKPTQHSVKELRSLGIQPDMVICRTHYELSQEIKEKLALFCDIDVNAVIENL
DVENIYEVPMALNEQRVDGLIADRLNLNSGSLELERWKQLVDQIKNLSQEITIGLVGKYVSLQDAYISVTEALDHAGFNY
DSKVKVKWINSEELEKNGSVETAFQGVDGILVPGGFGDRGIGGKIMAAQYARKNNIPFLGICLGMHCSVIDFAHEVAGLN
GAYSSEFHPETPYPVIDLLPEQKEVKDLGGTMRLGAYPCKLIEGTRAYEAYQQDIVQERHRHRYEFNNDYKEQLTNNGLV
VSGVSPDDRLVEIVELEDHPWFVATQFHPEFKSRPGAPHPLFNSFVQQALKNSLSSK

Sequences:

>Translated_537_residues
MSKYIFVTGGVASSLGKGISAASIGRLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFID
ENLSRINNITTGKIYWSVLSKERKGEYLGKTVQVIPHITDEIKSRIYQASKDNDADVVIAEIGGTVGDIESLPFLEAIRQ
MRSDIGRDSVMYIHVTLVPYLEKSGELKTKPTQHSVKELRSLGIQPDMVICRTHYELSQEIKEKLALFCDIDVNAVIENL
DVENIYEVPMALNEQRVDGLIADRLNLNSGSLELERWKQLVDQIKNLSQEITIGLVGKYVSLQDAYISVTEALDHAGFNY
DSKVKVKWINSEELEKNGSVETAFQGVDGILVPGGFGDRGIGGKIMAAQYARKNNIPFLGICLGMHCSVIDFAHEVAGLN
GAYSSEFHPETPYPVIDLLPEQKEVKDLGGTMRLGAYPCKLIEGTRAYEAYQQDIVQERHRHRYEFNNDYKEQLTNNGLV
VSGVSPDDRLVEIVELEDHPWFVATQFHPEFKSRPGAPHPLFNSFVQQALKNSLSSK
>Mature_536_residues
SKYIFVTGGVASSLGKGISAASIGRLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIDE
NLSRINNITTGKIYWSVLSKERKGEYLGKTVQVIPHITDEIKSRIYQASKDNDADVVIAEIGGTVGDIESLPFLEAIRQM
RSDIGRDSVMYIHVTLVPYLEKSGELKTKPTQHSVKELRSLGIQPDMVICRTHYELSQEIKEKLALFCDIDVNAVIENLD
VENIYEVPMALNEQRVDGLIADRLNLNSGSLELERWKQLVDQIKNLSQEITIGLVGKYVSLQDAYISVTEALDHAGFNYD
SKVKVKWINSEELEKNGSVETAFQGVDGILVPGGFGDRGIGGKIMAAQYARKNNIPFLGICLGMHCSVIDFAHEVAGLNG
AYSSEFHPETPYPVIDLLPEQKEVKDLGGTMRLGAYPCKLIEGTRAYEAYQQDIVQERHRHRYEFNNDYKEQLTNNGLVV
SGVSPDDRLVEIVELEDHPWFVATQFHPEFKSRPGAPHPLFNSFVQQALKNSLSSK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=566, Percent_Identity=45.7597173144876, Blast_Score=490, Evalue=1e-138,
Organism=Homo sapiens, GI28559083, Length=566, Percent_Identity=45.7597173144876, Blast_Score=490, Evalue=1e-138,
Organism=Homo sapiens, GI221316689, Length=566, Percent_Identity=45.7597173144876, Blast_Score=490, Evalue=1e-138,
Organism=Homo sapiens, GI148491070, Length=540, Percent_Identity=48.3333333333333, Blast_Score=484, Evalue=1e-136,
Organism=Escherichia coli, GI1789142, Length=538, Percent_Identity=56.3197026022305, Blast_Score=597, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI25148299, Length=602, Percent_Identity=39.8671096345515, Blast_Score=436, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319432, Length=559, Percent_Identity=43.4704830053667, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=44.1071428571429, Blast_Score=448, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24664469, Length=544, Percent_Identity=45.7720588235294, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI21357815, Length=485, Percent_Identity=43.7113402061856, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_NATTJ (B2A3K5)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001919022.1
- ProteinModelPortal:   B2A3K5
- SMR:   B2A3K5
- GeneID:   6315712
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2887
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60058; Mature: 59927

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 382-382 ACT_SITE 508-508 ACT_SITE 510-510

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYIFVTGGVASSLGKGISAASIGRLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVF
CCCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFIDENLSRINNITTGKIYWSVLSKERKGEYLGKTVQVIPHITD
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHH
EIKSRIYQASKDNDADVVIAEIGGTVGDIESLPFLEAIRQMRSDIGRDSVMYIHVTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEH
LEKSGELKTKPTQHSVKELRSLGIQPDMVICRTHYELSQEIKEKLALFCDIDVNAVIENL
HCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHEEECCCHHHHHHCC
DVENIYEVPMALNEQRVDGLIADRLNLNSGSLELERWKQLVDQIKNLSQEITIGLVGKYV
CHHHHHHCCHHCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCHHHEEEEEHCCHH
SLQDAYISVTEALDHAGFNYDSKVKVKWINSEELEKNGSVETAFQGVDGILVPGGFGDRG
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCCCCHHHHHCCCCCEEECCCCCCCC
IGGKIMAAQYARKNNIPFLGICLGMHCSVIDFAHEVAGLNGAYSSEFHPETPYPVIDLLP
CCCCCHHHHHHHCCCCCEEEEHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCC
EQKEVKDLGGTMRLGAYPCKLIEGTRAYEAYQQDIVQERHRHRYEFNNDYKEQLTNNGLV
CHHHHHHCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEE
VSGVSPDDRLVEIVELEDHPWFVATQFHPEFKSRPGAPHPLFNSFVQQALKNSLSSK
EECCCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKYIFVTGGVASSLGKGISAASIGRLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVF
CCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFIDENLSRINNITTGKIYWSVLSKERKGEYLGKTVQVIPHITD
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHH
EIKSRIYQASKDNDADVVIAEIGGTVGDIESLPFLEAIRQMRSDIGRDSVMYIHVTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEH
LEKSGELKTKPTQHSVKELRSLGIQPDMVICRTHYELSQEIKEKLALFCDIDVNAVIENL
HCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHEEECCCHHHHHHCC
DVENIYEVPMALNEQRVDGLIADRLNLNSGSLELERWKQLVDQIKNLSQEITIGLVGKYV
CHHHHHHCCHHCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCHHHEEEEEHCCHH
SLQDAYISVTEALDHAGFNYDSKVKVKWINSEELEKNGSVETAFQGVDGILVPGGFGDRG
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCCCCHHHHHCCCCCEEECCCCCCCC
IGGKIMAAQYARKNNIPFLGICLGMHCSVIDFAHEVAGLNGAYSSEFHPETPYPVIDLLP
CCCCCHHHHHHHCCCCCEEEEHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCC
EQKEVKDLGGTMRLGAYPCKLIEGTRAYEAYQQDIVQERHRHRYEFNNDYKEQLTNNGLV
CHHHHHHCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEE
VSGVSPDDRLVEIVELEDHPWFVATQFHPEFKSRPGAPHPLFNSFVQQALKNSLSSK
EECCCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA