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The map label for this gene is prfA

Identifier: 188587462

GI number: 188587462

Start: 3103797

End: 3104870

Strand: Reverse

Name: prfA

Synonym: Nther_2872

Alternate gene names: 188587462

Gene position: 3104870-3103797 (Counterclockwise)

Preceding gene: 188587463

Following gene: 188587461

Centisome position: 98.08

GC content: 37.9

Gene sequence:

>1074_bases
ATGCTAGATAAATTAGAAGGTATAGAAAAACGATATCAGGAACTGGAAGATTTAATGTCAGATCCTGAAGTTATAAATGA
CCCTGAAAAGTTAAAACAATATTCTAAAGAACATGCATCTCTTGAGGAAATCGTTAATAAATATCGACAATATCTTGAAA
CAAAACAAGAATTTGAAGAGGCTAAGGAAATGCTAGAAGAAAATGATGAAGATCCGGATATGGAAGAATTTTTAAAGGAT
GAAATTCCTCGACTGGAAAATCAAATGCAAGAGATCTATGACTCTCTTAAAATTCTGTTATTGCCCAAAGATCCCAATGA
TGAAAAGAACGTAATTGTTGAAATTAGAGCCGGAGCGGGTGGTGATGAAGCAGCTTTATTTGCTGCGGATCTGTATCGTA
TGTATACCCGCTATGCTGAGATAATGAACTGGAAAACAGAAATAATGGATAGTCATGAGAAAGATATGGGTGGCTTTAAA
GAGATTGTTTTCATGATAGAAGGTAAAAATGCTTATTCGCGTCTAAAATTTGAAAGTGGTGTCCACAGGGTACAGCGAGT
GCCCTCTACCGAATCTTCTGGTAGGATTCACACTTCAACTGCTACTGTTGCTGTATTGCCTGAAGCGGAAGATGTAGAAA
TTGAAATTGATACCAATGAACTCAGAATAGATACATTTTGTGCTACAGGACCAGGAGGGCAAAGTGTTAATACCACACAA
TCGGCAGTTAGAATCACCCATGAACCAACTGGAGTTGTTGTTAGTTGCCAGGATGAGAAATCTCAGCATAAGAATCGAGA
TAAAGCCATGAAAGTATTGCGAGCTAGAATATATGATATGTATCTGGAGGAACAACAACAGCAAGAGGCAGAAGAACGAA
AAAGTCAGGTGGGAAGTGGCGATAGGAGCGAAAGAATAAGAACTTACAACTTTCCCCAGGGGCGAATTACCGATCACAGG
ATAAACTTCACAACTCATAGACTGGAACAAGTGTTAGAAGGCGAAATCGATGAATTAATTGATGCTTTAATAACTACAGA
ACAAGCTGAACAATTGAAAAAAATGGGAGCCTAA

Upstream 100 bases:

>100_bases
AAAAGTTTACAACGAGAGAACCCGATGCTGAGCAGATAGAAGTGGCTTTGACTGCTTTAAACAACTTGATTTCTGAAAGT
GAGGAAAGAGAGGTGAATCC

Downstream 100 bases:

>100_bases
ACTATGGGAAGGTGTAAAAATGAAAGACTACGAAGCCGAATATGAAAATAGAGAAAATGACGTGACAGTGGCAGAGGCCC
GAAAATGGGCCTCTGATTAT

Product: peptide chain release factor 1 (bRF-1)

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLDKLEGIEKRYQELEDLMSDPEVINDPEKLKQYSKEHASLEEIVNKYRQYLETKQEFEEAKEMLEENDEDPDMEEFLKD
EIPRLENQMQEIYDSLKILLLPKDPNDEKNVIVEIRAGAGGDEAALFAADLYRMYTRYAEIMNWKTEIMDSHEKDMGGFK
EIVFMIEGKNAYSRLKFESGVHRVQRVPSTESSGRIHTSTATVAVLPEAEDVEIEIDTNELRIDTFCATGPGGQSVNTTQ
SAVRITHEPTGVVVSCQDEKSQHKNRDKAMKVLRARIYDMYLEEQQQQEAEERKSQVGSGDRSERIRTYNFPQGRITDHR
INFTTHRLEQVLEGEIDELIDALITTEQAEQLKKMGA

Sequences:

>Translated_357_residues
MLDKLEGIEKRYQELEDLMSDPEVINDPEKLKQYSKEHASLEEIVNKYRQYLETKQEFEEAKEMLEENDEDPDMEEFLKD
EIPRLENQMQEIYDSLKILLLPKDPNDEKNVIVEIRAGAGGDEAALFAADLYRMYTRYAEIMNWKTEIMDSHEKDMGGFK
EIVFMIEGKNAYSRLKFESGVHRVQRVPSTESSGRIHTSTATVAVLPEAEDVEIEIDTNELRIDTFCATGPGGQSVNTTQ
SAVRITHEPTGVVVSCQDEKSQHKNRDKAMKVLRARIYDMYLEEQQQQEAEERKSQVGSGDRSERIRTYNFPQGRITDHR
INFTTHRLEQVLEGEIDELIDALITTEQAEQLKKMGA
>Mature_357_residues
MLDKLEGIEKRYQELEDLMSDPEVINDPEKLKQYSKEHASLEEIVNKYRQYLETKQEFEEAKEMLEENDEDPDMEEFLKD
EIPRLENQMQEIYDSLKILLLPKDPNDEKNVIVEIRAGAGGDEAALFAADLYRMYTRYAEIMNWKTEIMDSHEKDMGGFK
EIVFMIEGKNAYSRLKFESGVHRVQRVPSTESSGRIHTSTATVAVLPEAEDVEIEIDTNELRIDTFCATGPGGQSVNTTQ
SAVRITHEPTGVVVSCQDEKSQHKNRDKAMKVLRARIYDMYLEEQQQQEAEERKSQVGSGDRSERIRTYNFPQGRITDHR
INFTTHRLEQVLEGEIDELIDALITTEQAEQLKKMGA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=293, Percent_Identity=46.4163822525597, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI34577120, Length=360, Percent_Identity=38.0555555555556, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI166795305, Length=198, Percent_Identity=42.4242424242424, Blast_Score=175, Evalue=6e-44,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=51.685393258427, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=38.2089552238806, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=284, Percent_Identity=34.8591549295775, Blast_Score=190, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=359, Percent_Identity=42.6183844011142, Blast_Score=249, Evalue=5e-67,
Organism=Drosophila melanogaster, GI19921226, Length=338, Percent_Identity=42.8994082840237, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_NATTJ (B2A3J0)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001919007.1
- ProteinModelPortal:   B2A3J0
- SMR:   B2A3J0
- GeneID:   6316330
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2872
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41236; Mature: 41236

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKLEGIEKRYQELEDLMSDPEVINDPEKLKQYSKEHASLEEIVNKYRQYLETKQEFEE
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEMLEENDEDPDMEEFLKDEIPRLENQMQEIYDSLKILLLPKDPNDEKNVIVEIRAGAG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCCC
GDEAALFAADLYRMYTRYAEIMNWKTEIMDSHEKDMGGFKEIVFMIEGKNAYSRLKFESG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHEEEEEECCCHHHHHHHHHH
VHRVQRVPSTESSGRIHTSTATVAVLPEAEDVEIEIDTNELRIDTFCATGPGGQSVNTTQ
HHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCEEEEEEEEECCCCCCCCCCHH
SAVRITHEPTGVVVSCQDEKSQHKNRDKAMKVLRARIYDMYLEEQQQQEAEERKSQVGSG
HHEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRITDHRINFTTHRLEQVLEGEIDELIDALITTEQAEQLKKMGA
CCCCCCEECCCCCCCCCCCEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDKLEGIEKRYQELEDLMSDPEVINDPEKLKQYSKEHASLEEIVNKYRQYLETKQEFEE
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKEMLEENDEDPDMEEFLKDEIPRLENQMQEIYDSLKILLLPKDPNDEKNVIVEIRAGAG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCCC
GDEAALFAADLYRMYTRYAEIMNWKTEIMDSHEKDMGGFKEIVFMIEGKNAYSRLKFESG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHEEEEEECCCHHHHHHHHHH
VHRVQRVPSTESSGRIHTSTATVAVLPEAEDVEIEIDTNELRIDTFCATGPGGQSVNTTQ
HHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCEEEEEEEEECCCCCCCCCCHH
SAVRITHEPTGVVVSCQDEKSQHKNRDKAMKVLRARIYDMYLEEQQQQEAEERKSQVGSG
HHEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRITDHRINFTTHRLEQVLEGEIDELIDALITTEQAEQLKKMGA
CCCCCCEECCCCCCCCCCCEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA