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The map label for this gene is ywkE [H]

Identifier: 188587461

GI number: 188587461

Start: 3102812

End: 3103777

Strand: Reverse

Name: ywkE [H]

Synonym: Nther_2871

Alternate gene names: 188587461

Gene position: 3103777-3102812 (Counterclockwise)

Preceding gene: 188587462

Following gene: 188587460

Centisome position: 98.05

GC content: 34.58

Gene sequence:

>966_bases
ATGAAAGACTACGAAGCCGAATATGAAAATAGAGAAAATGACGTGACAGTGGCAGAGGCCCGAAAATGGGCCTCTGATTA
TTTAAAAAGAGCCAAGATTGAAAATTACATTAAAGAAGCAGATTTCTTATTAGCATTTATCTTAGATTGGGATAGAAGCA
AATTATTAGCATATCCGGAAAAACATCTGAGCAGTGGTCAGTACAAGGAATTGAAAGAACTCGTAATTAAAAGATCTGAA
GGAACTCCCTATGCCTACTTAACGGGAAAAAGAGAGTTTATGGGACTTGAATTTACTGTTACAGAAAATGTGTTGATTCC
CAGACCTGATACAGAAGTGGTTGTGGAATTTGCTTTAAATTGTCTTGGAAAAATTATTTTGAAAGATAATCCTAATAATA
AAAAGGAATTACAATTTAATGAACTGAATTCTCCTATTAAAATATTGGATATCTGTACAGGTAGTGGCAACATTGGCCTT
TCAATTGCTTATTATTTTAACAAAATGTATGGGCAAAATTTAGAACTAACTCTATCAGATATCTCGGATAAAGCCTTAGA
GATAGCCCATATTAATGCTTCCAATTTAGGTTTGCTATCTCAATGTAAGTTTGTTAAAAGTGATTTATTTTCTAATACTT
CGGATGATAATTATCGATTAATAACAGCTAATCCCCCTTACATTTCTTCAAAACATTACTCGACACTATCTAAAGAGGTT
AAGTTGGAACCAGCTCATGCCCTACTAGCAGGGGAAGATGGTCTATATTATTATAAAAAAATCTCGCAACAGATTAAAAA
ATATCTATCCCAGGATGGGGTACTTATCTTTGAGATTGGCGAAGATCAGCAAGAAGAGGTTGAAAATATGCTTTCAACTC
AAAACCTTGTAGTGACAAGTGAACAAGATTTGGCAGGGCGACCACGGTTGATTGCAGCTAGTAGAGGATACGAACCATTT
GGTTAA

Upstream 100 bases:

>100_bases
TTAGAAGGCGAAATCGATGAATTAATTGATGCTTTAATAACTACAGAACAAGCTGAACAATTGAAAAAAATGGGAGCCTA
AACTATGGGAAGGTGTAAAA

Downstream 100 bases:

>100_bases
TGAACTAAATATTGCAACTTATTAAGATTTACCGGCGGGAAAACCGCCGGTTTTTTAATTTATGTGCTGTTATATTTAGT
CGTTGATACTGAAAAACTTT

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKDYEAEYENRENDVTVAEARKWASDYLKRAKIENYIKEADFLLAFILDWDRSKLLAYPEKHLSSGQYKELKELVIKRSE
GTPYAYLTGKREFMGLEFTVTENVLIPRPDTEVVVEFALNCLGKIILKDNPNNKKELQFNELNSPIKILDICTGSGNIGL
SIAYYFNKMYGQNLELTLSDISDKALEIAHINASNLGLLSQCKFVKSDLFSNTSDDNYRLITANPPYISSKHYSTLSKEV
KLEPAHALLAGEDGLYYYKKISQQIKKYLSQDGVLIFEIGEDQQEEVENMLSTQNLVVTSEQDLAGRPRLIAASRGYEPF
G

Sequences:

>Translated_321_residues
MKDYEAEYENRENDVTVAEARKWASDYLKRAKIENYIKEADFLLAFILDWDRSKLLAYPEKHLSSGQYKELKELVIKRSE
GTPYAYLTGKREFMGLEFTVTENVLIPRPDTEVVVEFALNCLGKIILKDNPNNKKELQFNELNSPIKILDICTGSGNIGL
SIAYYFNKMYGQNLELTLSDISDKALEIAHINASNLGLLSQCKFVKSDLFSNTSDDNYRLITANPPYISSKHYSTLSKEV
KLEPAHALLAGEDGLYYYKKISQQIKKYLSQDGVLIFEIGEDQQEEVENMLSTQNLVVTSEQDLAGRPRLIAASRGYEPF
G
>Mature_321_residues
MKDYEAEYENRENDVTVAEARKWASDYLKRAKIENYIKEADFLLAFILDWDRSKLLAYPEKHLSSGQYKELKELVIKRSE
GTPYAYLTGKREFMGLEFTVTENVLIPRPDTEVVVEFALNCLGKIILKDNPNNKKELQFNELNSPIKILDICTGSGNIGL
SIAYYFNKMYGQNLELTLSDISDKALEIAHINASNLGLLSQCKFVKSDLFSNTSDDNYRLITANPPYISSKHYSTLSKEV
KLEPAHALLAGEDGLYYYKKISQQIKKYLSQDGVLIFEIGEDQQEEVENMLSTQNLVVTSEQDLAGRPRLIAASRGYEPF
G

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=310, Percent_Identity=26.4516129032258, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87082085, Length=221, Percent_Identity=38.4615384615385, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1787463, Length=256, Percent_Identity=31.640625, Blast_Score=124, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24582226, Length=282, Percent_Identity=25.177304964539, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 36534; Mature: 36534

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDYEAEYENRENDVTVAEARKWASDYLKRAKIENYIKEADFLLAFILDWDRSKLLAYPE
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEECCH
KHLSSGQYKELKELVIKRSEGTPYAYLTGKREFMGLEFTVTENVLIPRPDTEVVVEFALN
HHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHEEEEEEEECCEEECCCCHHHHHHHHHH
CLGKIILKDNPNNKKELQFNELNSPIKILDICTGSGNIGLSIAYYFNKMYGQNLELTLSD
HHHEEEECCCCCCCCEEEHHHCCCCEEEEEEEECCCCCCEEHHEEHHHHHCCCEEEEEEC
ISDKALEIAHINASNLGLLSQCKFVKSDLFSNTSDDNYRLITANPPYISSKHYSTLSKEV
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHE
KLEPAHALLAGEDGLYYYKKISQQIKKYLSQDGVLIFEIGEDQQEEVENMLSTQNLVVTS
EECCHHEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEC
EQDLAGRPRLIAASRGYEPFG
CCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MKDYEAEYENRENDVTVAEARKWASDYLKRAKIENYIKEADFLLAFILDWDRSKLLAYPE
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEECCH
KHLSSGQYKELKELVIKRSEGTPYAYLTGKREFMGLEFTVTENVLIPRPDTEVVVEFALN
HHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHEEEEEEEECCEEECCCCHHHHHHHHHH
CLGKIILKDNPNNKKELQFNELNSPIKILDICTGSGNIGLSIAYYFNKMYGQNLELTLSD
HHHEEEECCCCCCCCEEEHHHCCCCEEEEEEEECCCCCCEEHHEEHHHHHCCCEEEEEEC
ISDKALEIAHINASNLGLLSQCKFVKSDLFSNTSDDNYRLITANPPYISSKHYSTLSKEV
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHE
KLEPAHALLAGEDGLYYYKKISQQIKKYLSQDGVLIFEIGEDQQEEVENMLSTQNLVVTS
EECCHHEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEC
EQDLAGRPRLIAASRGYEPFG
CCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]