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The map label for this gene is gcvH [H]

Identifier: 188587344

GI number: 188587344

Start: 2970295

End: 2970696

Strand: Reverse

Name: gcvH [H]

Synonym: Nther_2750

Alternate gene names: 188587344

Gene position: 2970696-2970295 (Counterclockwise)

Preceding gene: 188587345

Following gene: 188587343

Centisome position: 93.84

GC content: 34.83

Gene sequence:

>402_bases
ATGGTTAATGTACCAGAAGAATTTTATTATTCAAAGGATCATGAGTGGATTAAAAAACTTGAAGATGGGAAAGTTTGTGT
CGGAGTAACTGACTATGCTCAAGATCAATTGGGAGATATTGTCTTTGTAGAACTTCCTGAAGAAGAGGATGAATATGAAC
AGGAAGAAACATTTACGGTAATCGAATCTGTAAAAGCCGTAGCAGATACTTATGCTCCGGTATCTGGAACAATTGATGAA
ATAAATGAAGACTTAGAAGATAGTCCTGAATTAATTAATCAGGATCCTTATGGAGATGGTTGGATAGCTGTCTTTCAACT
TTCTGATGAGTCTGAGTTAGAAGAGTTATTGTCCGCTGAAGAATATCAAAATTATTTACAAGAAATTGAAGAGGAGGAAT
AG

Upstream 100 bases:

>100_bases
CTGAGCATGACCAAGAAGGTAATGAAATTGAAGTGAAGATAAGAAAGAAAACTGCAAAAGCAAAAATTGTAAAAACACCT
TTTTATAGGAGGGGATAAAA

Downstream 100 bases:

>100_bases
TTTTGAATAATAACTATTTACCTCATACTGACAATGAGCGAAGGGAAATGTTAGAGGCTATTGGGGTAGACTCGGTCCGT
GAGCTTTTTAAAGATATTCC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MVNVPEEFYYSKDHEWIKKLEDGKVCVGVTDYAQDQLGDIVFVELPEEEDEYEQEETFTVIESVKAVADTYAPVSGTIDE
INEDLEDSPELINQDPYGDGWIAVFQLSDESELEELLSAEEYQNYLQEIEEEE

Sequences:

>Translated_133_residues
MVNVPEEFYYSKDHEWIKKLEDGKVCVGVTDYAQDQLGDIVFVELPEEEDEYEQEETFTVIESVKAVADTYAPVSGTIDE
INEDLEDSPELINQDPYGDGWIAVFQLSDESELEELLSAEEYQNYLQEIEEEE
>Mature_133_residues
MVNVPEEFYYSKDHEWIKKLEDGKVCVGVTDYAQDQLGDIVFVELPEEEDEYEQEETFTVIESVKAVADTYAPVSGTIDE
INEDLEDSPELINQDPYGDGWIAVFQLSDESELEELLSAEEYQNYLQEIEEEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=122, Percent_Identity=42.6229508196721, Blast_Score=113, Evalue=4e-26,
Organism=Homo sapiens, GI89057342, Length=122, Percent_Identity=41.8032786885246, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1789271, Length=128, Percent_Identity=52.34375, Blast_Score=135, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=43.9655172413793, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=123, Percent_Identity=45.5284552845528, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=42.4, Blast_Score=111, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=38.2113821138211, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 15358; Mature: 15358

Theoretical pI: Translated: 3.32; Mature: 3.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVPEEFYYSKDHEWIKKLEDGKVCVGVTDYAQDQLGDIVFVELPEEEDEYEQEETFTV
CCCCCHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
IESVKAVADTYAPVSGTIDEINEDLEDSPELINQDPYGDGWIAVFQLSDESELEELLSAE
HHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHH
EYQNYLQEIEEEE
HHHHHHHHHHCCC
>Mature Secondary Structure
MVNVPEEFYYSKDHEWIKKLEDGKVCVGVTDYAQDQLGDIVFVELPEEEDEYEQEETFTV
CCCCCHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
IESVKAVADTYAPVSGTIDEINEDLEDSPELINQDPYGDGWIAVFQLSDESELEELLSAE
HHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHH
EYQNYLQEIEEEE
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA