Click here to switch to the map view.

The map label for this gene is gcvT

Identifier: 188587345

GI number: 188587345

Start: 2970699

End: 2971796

Strand: Reverse

Name: gcvT

Synonym: Nther_2751

Alternate gene names: 188587345

Gene position: 2971796-2970699 (Counterclockwise)

Preceding gene: 188587346

Following gene: 188587344

Centisome position: 93.88

GC content: 37.52

Gene sequence:

>1098_bases
TTGACCCACCCACAAAAAACCCCTTTGTATGATATCCATAAAGAACGAGGGGGAAAGATAATTGACTTCGGTGGTTGGTA
CTTGCCGGTACAGTTCACAGGTATTATTGACGAGGTAATGACAACTAGAAAAGAGGCCGGTCTATTTGATGTTTCCCACA
TGGGAGAAATAATAGTAGAAGGCCCAAAGGCTTTAGAGTATCTTCAAAAAATGGTCCCTAATGATGTGGCCAGATTAAAA
CCGGGTAAGATACTTTACACACCAATGTGCTATGAAAACGGTGGAACTGTAGATGACTTTTTAATTTATAAAATGGATGA
AAATAAATTCCTTCTTATTGTTAATGCAGCTAACACGGACAAGGATTTTGAATGGCTTCAGGAAAATAATACTGAAGGTG
TTGAATTGAAAAATCTTTCTGATGAATATGGTCAGATAGCGATTCAAGGTCCCAAGGCCGAAAAAATATTACAACGCTTG
ACGGATACTCCTCTTAAAGAGATTAAATTTTTTAATTTCAAGGAAGATGTGGATTTAGATGGTGTGAAAGCTTTAATTTC
CCGCACTGGTTATACTGGGGAAAATGGTTTTGAAATTTATATCAAGGCAGAAGAAACAGCCAAACTTTGGGAAAAGATTG
AAGATGCCGGTGAAAATGATGGATTAAAGCCAATTGGTCTTGGAGCTAGGGATGTACTTAGATTTGAAGTTTGTTTGCCA
TTATACGGTAATGAATTGTCACCTGAAATTACCCCCCTAGAAGCCAGACTGAATCCTTTTGTTAAGTTGAATAAAACAGA
AGACTTTTTAGGCAAAGATGTGTTAGTGAACCAAAAAGAACAAGGTCTAGAAAGGGTTCTTGTAGGATTTGAGATGATCG
ATAGGGGAATTCCAAGAACCAATTATATCTTGATGAAAGACGGACAGGAGATTGGCTTTGTTAGTTCGGGATCACAATCA
CCAACTCTTGATAAAGCCCTAGGGCTTGGCTTCATTAAGCCTGAGCATGACCAAGAAGGTAATGAAATTGAAGTGAAGAT
AAGAAAGAAAACTGCAAAAGCAAAAATTGTAAAAACACCTTTTTATAGGAGGGGATAA

Upstream 100 bases:

>100_bases
TTTAAGTTCACAAAATAACTAGATTGGGAGCAAGAGGAATTTTTTATTTTTTGTATAATATATTTAATTGCAGAGGTGAT
TAGATTATGGAGGTGATTTT

Downstream 100 bases:

>100_bases
AAATGGTTAATGTACCAGAAGAATTTTATTATTCAAAGGATCATGAGTGGATTAAAAAACTTGAAGATGGGAAAGTTTGT
GTCGGAGTAACTGACTATGC

Product: aminomethyltransferase

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTHPQKTPLYDIHKERGGKIIDFGGWYLPVQFTGIIDEVMTTRKEAGLFDVSHMGEIIVEGPKALEYLQKMVPNDVARLK
PGKILYTPMCYENGGTVDDFLIYKMDENKFLLIVNAANTDKDFEWLQENNTEGVELKNLSDEYGQIAIQGPKAEKILQRL
TDTPLKEIKFFNFKEDVDLDGVKALISRTGYTGENGFEIYIKAEETAKLWEKIEDAGENDGLKPIGLGARDVLRFEVCLP
LYGNELSPEITPLEARLNPFVKLNKTEDFLGKDVLVNQKEQGLERVLVGFEMIDRGIPRTNYILMKDGQEIGFVSSGSQS
PTLDKALGLGFIKPEHDQEGNEIEVKIRKKTAKAKIVKTPFYRRG

Sequences:

>Translated_365_residues
MTHPQKTPLYDIHKERGGKIIDFGGWYLPVQFTGIIDEVMTTRKEAGLFDVSHMGEIIVEGPKALEYLQKMVPNDVARLK
PGKILYTPMCYENGGTVDDFLIYKMDENKFLLIVNAANTDKDFEWLQENNTEGVELKNLSDEYGQIAIQGPKAEKILQRL
TDTPLKEIKFFNFKEDVDLDGVKALISRTGYTGENGFEIYIKAEETAKLWEKIEDAGENDGLKPIGLGARDVLRFEVCLP
LYGNELSPEITPLEARLNPFVKLNKTEDFLGKDVLVNQKEQGLERVLVGFEMIDRGIPRTNYILMKDGQEIGFVSSGSQS
PTLDKALGLGFIKPEHDQEGNEIEVKIRKKTAKAKIVKTPFYRRG
>Mature_364_residues
THPQKTPLYDIHKERGGKIIDFGGWYLPVQFTGIIDEVMTTRKEAGLFDVSHMGEIIVEGPKALEYLQKMVPNDVARLKP
GKILYTPMCYENGGTVDDFLIYKMDENKFLLIVNAANTDKDFEWLQENNTEGVELKNLSDEYGQIAIQGPKAEKILQRLT
DTPLKEIKFFNFKEDVDLDGVKALISRTGYTGENGFEIYIKAEETAKLWEKIEDAGENDGLKPIGLGARDVLRFEVCLPL
YGNELSPEITPLEARLNPFVKLNKTEDFLGKDVLVNQKEQGLERVLVGFEMIDRGIPRTNYILMKDGQEIGFVSSGSQSP
TLDKALGLGFIKPEHDQEGNEIEVKIRKKTAKAKIVKTPFYRRG

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=370, Percent_Identity=32.7027027027027, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI257796258, Length=356, Percent_Identity=32.3033707865169, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI257796254, Length=364, Percent_Identity=30.7692307692308, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI24797151, Length=344, Percent_Identity=29.3604651162791, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI257796256, Length=313, Percent_Identity=30.3514376996805, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI197927446, Length=342, Percent_Identity=27.1929824561404, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21361378, Length=342, Percent_Identity=27.1929824561404, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194306651, Length=329, Percent_Identity=26.4437689969605, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI1789272, Length=377, Percent_Identity=35.8090185676393, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17560118, Length=371, Percent_Identity=33.9622641509434, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI32563613, Length=348, Percent_Identity=24.4252873563218, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71994052, Length=323, Percent_Identity=25.6965944272446, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994045, Length=323, Percent_Identity=25.6965944272446, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320222, Length=387, Percent_Identity=34.1085271317829, Blast_Score=193, Evalue=3e-50,
Organism=Drosophila melanogaster, GI20129441, Length=377, Percent_Identity=33.42175066313, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28571104, Length=332, Percent_Identity=28.3132530120482, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI20130091, Length=351, Percent_Identity=25.9259259259259, Blast_Score=104, Evalue=7e-23,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_NATTJ (B2A2T4)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918890.1
- ProteinModelPortal:   B2A2T4
- SMR:   B2A2T4
- GeneID:   6314345
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2751
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 41270; Mature: 41139

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPQKTPLYDIHKERGGKIIDFGGWYLPVQFTGIIDEVMTTRKEAGLFDVSHMGEIIVE
CCCCCCCCCHHHHHHCCCEEEEECCEEEEEEEHHHHHHHHHHHHHCCCEEHHHCCCEEEC
GPKALEYLQKMVPNDVARLKPGKILYTPMCYENGGTVDDFLIYKMDENKFLLIVNAANTD
CHHHHHHHHHHCCCHHHHCCCCCEEEEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCC
KDFEWLQENNTEGVELKNLSDEYGQIAIQGPKAEKILQRLTDTPLKEIKFFNFKEDVDLD
CHHHHHHCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHEECCCCCCCCHH
GVKALISRTGYTGENGFEIYIKAEETAKLWEKIEDAGENDGLKPIGLGARDVLRFEVCLP
HHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCEECCCHHHHEEEEEEEE
LYGNELSPEITPLEARLNPFVKLNKTEDFLGKDVLVNQKEQGLERVLVGFEMIDRGIPRT
ECCCCCCCCCCCHHHCCCCCEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
NYILMKDGQEIGFVSSGSQSPTLDKALGLGFIKPEHDQEGNEIEVKIRKKTAKAKIVKTP
CEEEEECCCEECEEECCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECC
FYRRG
CCCCC
>Mature Secondary Structure 
THPQKTPLYDIHKERGGKIIDFGGWYLPVQFTGIIDEVMTTRKEAGLFDVSHMGEIIVE
CCCCCCCCHHHHHHCCCEEEEECCEEEEEEEHHHHHHHHHHHHHCCCEEHHHCCCEEEC
GPKALEYLQKMVPNDVARLKPGKILYTPMCYENGGTVDDFLIYKMDENKFLLIVNAANTD
CHHHHHHHHHHCCCHHHHCCCCCEEEEEEEECCCCCEEEEEEEEECCCCEEEEEECCCCC
KDFEWLQENNTEGVELKNLSDEYGQIAIQGPKAEKILQRLTDTPLKEIKFFNFKEDVDLD
CHHHHHHCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHEECCCCCCCCHH
GVKALISRTGYTGENGFEIYIKAEETAKLWEKIEDAGENDGLKPIGLGARDVLRFEVCLP
HHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCEECCCHHHHEEEEEEEE
LYGNELSPEITPLEARLNPFVKLNKTEDFLGKDVLVNQKEQGLERVLVGFEMIDRGIPRT
ECCCCCCCCCCCHHHCCCCCEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
NYILMKDGQEIGFVSSGSQSPTLDKALGLGFIKPEHDQEGNEIEVKIRKKTAKAKIVKTP
CEEEEECCCEECEEECCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCEEEEECC
FYRRG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA