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The map label for this gene is ldh [H]

Identifier: 188587298

GI number: 188587298

Start: 2914515

End: 2915603

Strand: Reverse

Name: ldh [H]

Synonym: Nther_2701

Alternate gene names: 188587298

Gene position: 2915603-2914515 (Counterclockwise)

Preceding gene: 188587299

Following gene: 188587297

Centisome position: 92.1

GC content: 38.38

Gene sequence:

>1089_bases
ATGTCTGAACAGGTTTTTAGGGAAATGGAGCAACAGGGTCATGAACAGGTGATTTTTAATTATGACAAAAAAACTGGTTT
AAAAGCTATTATTGCAATCCACGACACTACTTTGGGCCCGGCTCTTGGTGGTTGCCGGATGATGGACTACGAAAGCGATG
ATGATGCTTTAACCGATGTCCTTCGCTTATCTAAAGGTATGACCTACAAATGTGGAGTTTCAGGTGTGGATTATGGTGGT
GGAAAGACAGTTATGATTGGAGATCCAGAACAAAAAAATGAAGGAATGTTCAGAGCCCTGGGACGTTTTGTTCAAACTAT
GCGAGGACGTTATTACACTGGTACTGACGTGGGAACATCCCCAGACGATTTTATTCACTCTATGAGAGAAACAGATTGTA
TAGTAGGACGTCCTGTAGAATTCGGTGGTGGCGGAAGTACTGCTATTACCACGGCCTATGGTGTCTGGCAGGGATTGAAG
GCTTCTGCTAAAGCAAAATACGGAACTGATTCGCTATCAGGAAAAAAAGTTGCCGTCCAAGGTTTAGGAAAAGTTGGGCA
ATTACTTGTTAAACATTTAATTGAAGATGAAGGGGCTGAGGTAGTGGCAACTGATATTAGTAAAGATGCCATTGAAGAAT
TGAAATCGAAATATTCACAGATTGAAACGTGTGAACCGGATCAGATTTATGAAGTGGATTGCGATATTTTCAGTCCCAAT
GCATTAGGTGCAGTTATCAATGACTTCACTATTGAAAAATTAAAATGTGATATTGTAGCGGGTTCAGCAAATAATCAGTT
ACAAGAGGATAAACACGGTGATATTTTATTTGAAAAGGGAATTTTATATGCACCGGATTATGTAATTAATGCCGGAGGGC
TAATTCAAGCAGCTGATGAAGTCAAAGAATTCAATCATGACAGAGTTATGAAAACTACGGCTAGAATATATGAAATATTG
TTAGACATTTTCAAAATATCTGAAGAAAAGCGAGTACCTACTTATCAAGCAGCCAATATATTAGTAGAAGATCGATTAGA
TACAGTAGCAGAGGTCAGAAAAAACTTTTTAGTTGAAGAAAAGAAATAA

Upstream 100 bases:

>100_bases
AATTTTTATCTTTTTATGGAACACCGTTCCATACAATGGAACTATAAATAAATTATGAAGAGTTAATATTTTAAAAAATC
TTTTCAAGGAGGTTTTTAAG

Downstream 100 bases:

>100_bases
CGAAAATACTTTAACGAAAATATTAATATAAAATGTTCGAACTTTAAAAATCTTATTATAGGTTCCTGGATCAGTCCAGG
TCCAGGAACCTATAGGCATG

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSEQVFREMEQQGHEQVIFNYDKKTGLKAIIAIHDTTLGPALGGCRMMDYESDDDALTDVLRLSKGMTYKCGVSGVDYGG
GKTVMIGDPEQKNEGMFRALGRFVQTMRGRYYTGTDVGTSPDDFIHSMRETDCIVGRPVEFGGGGSTAITTAYGVWQGLK
ASAKAKYGTDSLSGKKVAVQGLGKVGQLLVKHLIEDEGAEVVATDISKDAIEELKSKYSQIETCEPDQIYEVDCDIFSPN
ALGAVINDFTIEKLKCDIVAGSANNQLQEDKHGDILFEKGILYAPDYVINAGGLIQAADEVKEFNHDRVMKTTARIYEIL
LDIFKISEEKRVPTYQAANILVEDRLDTVAEVRKNFLVEEKK

Sequences:

>Translated_362_residues
MSEQVFREMEQQGHEQVIFNYDKKTGLKAIIAIHDTTLGPALGGCRMMDYESDDDALTDVLRLSKGMTYKCGVSGVDYGG
GKTVMIGDPEQKNEGMFRALGRFVQTMRGRYYTGTDVGTSPDDFIHSMRETDCIVGRPVEFGGGGSTAITTAYGVWQGLK
ASAKAKYGTDSLSGKKVAVQGLGKVGQLLVKHLIEDEGAEVVATDISKDAIEELKSKYSQIETCEPDQIYEVDCDIFSPN
ALGAVINDFTIEKLKCDIVAGSANNQLQEDKHGDILFEKGILYAPDYVINAGGLIQAADEVKEFNHDRVMKTTARIYEIL
LDIFKISEEKRVPTYQAANILVEDRLDTVAEVRKNFLVEEKK
>Mature_361_residues
SEQVFREMEQQGHEQVIFNYDKKTGLKAIIAIHDTTLGPALGGCRMMDYESDDDALTDVLRLSKGMTYKCGVSGVDYGGG
KTVMIGDPEQKNEGMFRALGRFVQTMRGRYYTGTDVGTSPDDFIHSMRETDCIVGRPVEFGGGGSTAITTAYGVWQGLKA
SAKAKYGTDSLSGKKVAVQGLGKVGQLLVKHLIEDEGAEVVATDISKDAIEELKSKYSQIETCEPDQIYEVDCDIFSPNA
LGAVINDFTIEKLKCDIVAGSANNQLQEDKHGDILFEKGILYAPDYVINAGGLIQAADEVKEFNHDRVMKTTARIYEILL
DIFKISEEKRVPTYQAANILVEDRLDTVAEVRKNFLVEEKK

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=347, Percent_Identity=26.5129682997118, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4885281, Length=346, Percent_Identity=24.5664739884393, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45549226, Length=332, Percent_Identity=27.710843373494, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45553475, Length=332, Percent_Identity=27.710843373494, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24649460, Length=285, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24649283, Length=387, Percent_Identity=25.3229974160207, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 39888; Mature: 39756

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQVFREMEQQGHEQVIFNYDKKTGLKAIIAIHDTTLGPALGGCRMMDYESDDDALTDV
CCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCHHHHHHH
LRLSKGMTYKCGVSGVDYGGGKTVMIGDPEQKNEGMFRALGRFVQTMRGRYYTGTDVGTS
HHHHCCCEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCC
PDDFIHSMRETDCIVGRPVEFGGGGSTAITTAYGVWQGLKASAKAKYGTDSLSGKKVAVQ
HHHHHHHHHHCCCEECCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHH
GLGKVGQLLVKHLIEDEGAEVVATDISKDAIEELKSKYSQIETCEPDQIYEVDCDIFSPN
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCEECCC
ALGAVINDFTIEKLKCDIVAGSANNQLQEDKHGDILFEKGILYAPDYVINAGGLIQAADE
HHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCEEEECCCEECCCEEECCCCHHHHHHH
VKEFNHDRVMKTTARIYEILLDIFKISEEKRVPTYQAANILVEDRLDTVAEVRKNFLVEE
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KK
CC
>Mature Secondary Structure 
SEQVFREMEQQGHEQVIFNYDKKTGLKAIIAIHDTTLGPALGGCRMMDYESDDDALTDV
CHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCHHHHHHH
LRLSKGMTYKCGVSGVDYGGGKTVMIGDPEQKNEGMFRALGRFVQTMRGRYYTGTDVGTS
HHHHCCCEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCC
PDDFIHSMRETDCIVGRPVEFGGGGSTAITTAYGVWQGLKASAKAKYGTDSLSGKKVAVQ
HHHHHHHHHHCCCEECCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHH
GLGKVGQLLVKHLIEDEGAEVVATDISKDAIEELKSKYSQIETCEPDQIYEVDCDIFSPN
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCEECCC
ALGAVINDFTIEKLKCDIVAGSANNQLQEDKHGDILFEKGILYAPDYVINAGGLIQAADE
HHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCEEEECCCEECCCEEECCCCHHHHHHH
VKEFNHDRVMKTTARIYEILLDIFKISEEKRVPTYQAANILVEDRLDTVAEVRKNFLVEE
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]