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The map label for this gene is mdeA [H]

Identifier: 188587275

GI number: 188587275

Start: 2885893

End: 2887077

Strand: Direct

Name: mdeA [H]

Synonym: Nther_2677

Alternate gene names: 188587275

Gene position: 2885893-2887077 (Clockwise)

Preceding gene: 188587269

Following gene: 188587278

Centisome position: 91.17

GC content: 41.01

Gene sequence:

>1185_bases
TTGGCTAGAGAGTACAAGCATAAATTTGAGACTCGTGCCGTTCACGCTGATGAAGGTCACAAAAATGAGGTCGGTGCTCT
AAGAACTCCTATATACCAAAGCTCAACCTTCGTATTTGACAACGTGGAACAAGGTGCCAATAGATTTTGTGGAGAAGAAG
CAGGATATATTTATACCAGGCTAGGTAATCCCACACAAAGAGATTTGGAAAAGCAAATGGCCAATTTAGAAGGAGCTGAG
GACGCTATTGCTGTTGGGTCTGGAATGGCGGCTGTTTCCGCTGTTGTGATGAATTTATTAAATAGTGGAGACCATATGGT
AGCATCTGACACACTTTACGGTTGCACACATTCACTATTTGACGAATTATTGCCCAACTGGAATGTGGACGTAACTTTTG
TCGATATGACAGATCCCGATAATGTCCGAAATGCCATCAGGAACAGTACCAAAGTAATTTATACAGAAACCCCTGCAAAT
CCTACCCTTTCAATCATTGACCTAAACGAAATTGTTAATATAGCCAAGGATAAAAATATCACTACTATTACTGATAATAC
TTTTATGACCCCCTATTTACAAAGACCTATTGAAATGGGAATAGATGTGGTAATTCACAGTGCCACCAAGTATCTAGGTG
GTCACGGAGATACTATTGGTGGTGTGATTGTGGGCTCCCAGGAACTTATGAACGAACTTAGAATGACTACAGTCAAAGAT
TTTGGCGGCATTATTGGTCCTTTTGATGCCTTTCTACTCTTACGGGGTATTAAAACCTTGACAGTTCGAATGGATAAGAT
TTGTGAGAATGCCAAAAAGATAGCCAAGTTTTTAGAAGAACATTCAGAAGTAGAAAGAGTTTACTATCCAGGCTTAGAAA
GCCATCCTCAACACTCCCTTGCTAAAAAACAAATGGGAGATTTCGGTGGCATGATCACCTTTGAACTAAAAGGTGGCTAT
GAAGCAGGTAAAAAACTGATGAACAGTGTAGAACTTTGTGTACTGGCCGTTAGCTTGGGCGATGTGGATACCTTAATTCA
GCATCCCGCTTCCATGACTCACTATGTAGTACCTCCAGAAGAAAGAGAAAAAGCAGGCATCACCGACGGCCTAGTCAGGC
TGTCGGTAGGAATTGAAGATCCGGAAGATGTCATGAATGATTTAAAACAAGGCATGGAAAGTTAA

Upstream 100 bases:

>100_bases
TTTTAAATATTTGCGCAATAATTGTTAATCTGGCACGGCAGTTGCAACTAAGGTATAATTAGAAAATTCTAAAACTTATC
GAAAAATAGGAGGTGTTAAT

Downstream 100 bases:

>100_bases
AATTAAATAAACAGCAGATAAGTGAATTTAGTGAATAATACTTTGAGAATTGATGGGACAATCATCTTTAATCTTCAACT
ATCTCCAATGCCCCCGATTG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAREYKHKFETRAVHADEGHKNEVGALRTPIYQSSTFVFDNVEQGANRFCGEEAGYIYTRLGNPTQRDLEKQMANLEGAE
DAIAVGSGMAAVSAVVMNLLNSGDHMVASDTLYGCTHSLFDELLPNWNVDVTFVDMTDPDNVRNAIRNSTKVIYTETPAN
PTLSIIDLNEIVNIAKDKNITTITDNTFMTPYLQRPIEMGIDVVIHSATKYLGGHGDTIGGVIVGSQELMNELRMTTVKD
FGGIIGPFDAFLLLRGIKTLTVRMDKICENAKKIAKFLEEHSEVERVYYPGLESHPQHSLAKKQMGDFGGMITFELKGGY
EAGKKLMNSVELCVLAVSLGDVDTLIQHPASMTHYVVPPEEREKAGITDGLVRLSVGIEDPEDVMNDLKQGMES

Sequences:

>Translated_394_residues
MAREYKHKFETRAVHADEGHKNEVGALRTPIYQSSTFVFDNVEQGANRFCGEEAGYIYTRLGNPTQRDLEKQMANLEGAE
DAIAVGSGMAAVSAVVMNLLNSGDHMVASDTLYGCTHSLFDELLPNWNVDVTFVDMTDPDNVRNAIRNSTKVIYTETPAN
PTLSIIDLNEIVNIAKDKNITTITDNTFMTPYLQRPIEMGIDVVIHSATKYLGGHGDTIGGVIVGSQELMNELRMTTVKD
FGGIIGPFDAFLLLRGIKTLTVRMDKICENAKKIAKFLEEHSEVERVYYPGLESHPQHSLAKKQMGDFGGMITFELKGGY
EAGKKLMNSVELCVLAVSLGDVDTLIQHPASMTHYVVPPEEREKAGITDGLVRLSVGIEDPEDVMNDLKQGMES
>Mature_393_residues
AREYKHKFETRAVHADEGHKNEVGALRTPIYQSSTFVFDNVEQGANRFCGEEAGYIYTRLGNPTQRDLEKQMANLEGAED
AIAVGSGMAAVSAVVMNLLNSGDHMVASDTLYGCTHSLFDELLPNWNVDVTFVDMTDPDNVRNAIRNSTKVIYTETPANP
TLSIIDLNEIVNIAKDKNITTITDNTFMTPYLQRPIEMGIDVVIHSATKYLGGHGDTIGGVIVGSQELMNELRMTTVKDF
GGIIGPFDAFLLLRGIKTLTVRMDKICENAKKIAKFLEEHSEVERVYYPGLESHPQHSLAKKQMGDFGGMITFELKGGYE
AGKKLMNSVELCVLAVSLGDVDTLIQHPASMTHYVVPPEEREKAGITDGLVRLSVGIEDPEDVMNDLKQGMES

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=377, Percent_Identity=39.5225464190981, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI299473759, Length=377, Percent_Identity=36.604774535809, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI25453487, Length=376, Percent_Identity=34.5744680851064, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI1790375, Length=389, Percent_Identity=36.5038560411311, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=28.2442748091603, Blast_Score=168, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17560056, Length=376, Percent_Identity=43.6170212765958, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17538055, Length=376, Percent_Identity=42.5531914893617, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=42.2764227642276, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI193202265, Length=88, Percent_Identity=37.5, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319307, Length=401, Percent_Identity=38.1546134663342, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=32.648401826484, Blast_Score=222, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6321254, Length=389, Percent_Identity=33.1619537275064, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321170, Length=323, Percent_Identity=30.030959752322, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=27.3869346733668, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322590, Length=272, Percent_Identity=25.3676470588235, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19922554, Length=390, Percent_Identity=38.2051282051282, Blast_Score=281, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43424; Mature: 43293

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREYKHKFETRAVHADEGHKNEVGALRTPIYQSSTFVFDNVEQGANRFCGEEAGYIYTR
CCCHHHHHHHHHEEECCCCCCCCCCHHHCCCCCCCEEEECCHHHHHHHHCCCCCCEEEEE
LGNPTQRDLEKQMANLEGAEDAIAVGSGMAAVSAVVMNLLNSGDHMVASDTLYGCTHSLF
CCCCHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHH
DELLPNWNVDVTFVDMTDPDNVRNAIRNSTKVIYTETPANPTLSIIDLNEIVNIAKDKNI
HHHCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHCCCCE
TTITDNTFMTPYLQRPIEMGIDVVIHSATKYLGGHGDTIGGVIVGSQELMNELRMTTVKD
EEEECCCEECHHHHCHHHCCHHHEEHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHH
FGGIIGPFDAFLLLRGIKTLTVRMDKICENAKKIAKFLEEHSEVERVYYPGLESHPQHSL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AKKQMGDFGGMITFELKGGYEAGKKLMNSVELCVLAVSLGDVDTLIQHPASMTHYVVPPE
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCC
EREKAGITDGLVRLSVGIEDPEDVMNDLKQGMES
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AREYKHKFETRAVHADEGHKNEVGALRTPIYQSSTFVFDNVEQGANRFCGEEAGYIYTR
CCHHHHHHHHHEEECCCCCCCCCCHHHCCCCCCCEEEECCHHHHHHHHCCCCCCEEEEE
LGNPTQRDLEKQMANLEGAEDAIAVGSGMAAVSAVVMNLLNSGDHMVASDTLYGCTHSLF
CCCCHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHH
DELLPNWNVDVTFVDMTDPDNVRNAIRNSTKVIYTETPANPTLSIIDLNEIVNIAKDKNI
HHHCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHCCCCE
TTITDNTFMTPYLQRPIEMGIDVVIHSATKYLGGHGDTIGGVIVGSQELMNELRMTTVKD
EEEECCCEECHHHHCHHHCCHHHEEHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHH
FGGIIGPFDAFLLLRGIKTLTVRMDKICENAKKIAKFLEEHSEVERVYYPGLESHPQHSL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AKKQMGDFGGMITFELKGGYEAGKKLMNSVELCVLAVSLGDVDTLIQHPASMTHYVVPPE
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCC
EREKAGITDGLVRLSVGIEDPEDVMNDLKQGMES
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]