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The map label for this gene is ansB [H]

Identifier: 188587265

GI number: 188587265

Start: 2872332

End: 2873771

Strand: Reverse

Name: ansB [H]

Synonym: Nther_2667

Alternate gene names: 188587265

Gene position: 2873771-2872332 (Counterclockwise)

Preceding gene: 188587266

Following gene: 188587264

Centisome position: 90.78

GC content: 37.78

Gene sequence:

>1440_bases
ATGAATAGCGAAGATGTGAAAGAAATAACAAGAGTAGAGTGCGATTCTTTAGGGTCAAAAGAAATACCTAAAGATGCCTA
CTATGGGATTCAGTCAAAAAGAGCCCAAGAAAATTTTCCGTTATCTGGATATAGAGTACATCCAGAACTGATTAAAGCAA
TGGGTAAAGTGAAGTATGCATCTGTTGTAGCCAATATGAAAACAGGAAAAATGCCCAACAAATCAGGAGAGGCTATTAAA
CAGGCTTGTGAAGAAATGAGTTCAGGTAAATTTTTCGATGCTATTGTGGTTGAGGCAATTCAAGGTGGTGCTGGCACATC
AATAAATATGAACGTAAACGAAATTCTCGCTAATCGCGCTATTGAAATATTAGGCCATGAAAAGGGTGATTACGACGTAG
TTTCACCTAACACTCATGTAAATATGTCCCAATCCACCAATGATGTAGTACCTACATCATTTAAATTGGCTGTTATTACT
ATGCGTGAAGATCTTATCAGTAAGCTAGAAAATTTATATCAAACATTGTCCAAGAAAGAACAAGAATTTGATAAAGTTAT
CAAGACGGGTAGAACCCATCTTCAGGATGCAGTTCCCATCCGACTTGGACAAGAGTTCGGAGCTTATGCCAGGTTTGTAA
AACGCGATATTGAACGGATTTCTAATTCAATAGAAGCTCTCAAAACAGTAAATTTAGGTGCTACAGCTGTTGGTACTGGT
TTAAATGCCGACTCTAAATACATTAATACTGCTATAGAAGAATTAAGGGAAATCACTGGTATAGATCTAGAAATCAGTGA
TCATCTAGTTGATGCAACACAAAATACAGATGCCTATGTTCATGTATCCGGTGCTTTAAAAACAGCAGCTCTTAACATGT
CCAAAATGGCCAATGATTTGCGCTTGATGTCTTCAGGTCCGAGATGTGGCTTTAAGGAGATTAAGCTGCCAGCTGTTCAG
CCAGGTTCTTCAATTATGCCTGGTAAGGTGAATCCCGTAATTCCAGAAGTTATCAATCAAATCTGCTTCCAGGTAGCAGG
AAATGACCATACTATTTCAATGGCTTCAGAAGCAGGTCAATTGGAACTTAATGTGATGGGCCCGGTTTTATTTAGGAATA
TGTTTGAATCCATGGAGATAATAGCCAATGGAGCTCATATACTGTCTGAAAAATGTATTAAAGGCATTGAGCCCAATCAT
GATAGATGTAATGAGTTAGTTGAAAATAGTATTGGAATTATTACAGCCTTAAATCCTCATATAGGATATGAAACGGCTTC
CGAAATTGCCAAGGAAGTTTTAGAAACCGGTCGTAGTGTACGAGATGTCATTCTAGAACGAGAAATCCTAACAGAGGAAG
AAATTGAAGACATTCTAGATCCCAAGGAAATGACTGAACCAGGTATTGCTGGAAAAAAGAAAAATGACGTTACTGCTTAA

Upstream 100 bases:

>100_bases
AAACTATGTCTCAAAATTTTGCACGAAGGGCGTAATACACTGTTTTTGCAGCGCCTGAAAGGTAATTGCTATAACATATA
ATTTAGGAGGGGGTTCCTTA

Downstream 100 bases:

>100_bases
AACTTAAGAGGACAAGCCATGTTATAAACTGAGGCGCTGTTATATTTAGCTGTTGATACTGTGAAGTTGAAATTTTACAG
TTATCAACATTCAGCTTTAA

Product: fumarase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNSEDVKEITRVECDSLGSKEIPKDAYYGIQSKRAQENFPLSGYRVHPELIKAMGKVKYASVVANMKTGKMPNKSGEAIK
QACEEMSSGKFFDAIVVEAIQGGAGTSINMNVNEILANRAIEILGHEKGDYDVVSPNTHVNMSQSTNDVVPTSFKLAVIT
MREDLISKLENLYQTLSKKEQEFDKVIKTGRTHLQDAVPIRLGQEFGAYARFVKRDIERISNSIEALKTVNLGATAVGTG
LNADSKYINTAIEELREITGIDLEISDHLVDATQNTDAYVHVSGALKTAALNMSKMANDLRLMSSGPRCGFKEIKLPAVQ
PGSSIMPGKVNPVIPEVINQICFQVAGNDHTISMASEAGQLELNVMGPVLFRNMFESMEIIANGAHILSEKCIKGIEPNH
DRCNELVENSIGIITALNPHIGYETASEIAKEVLETGRSVRDVILEREILTEEEIEDILDPKEMTEPGIAGKKKNDVTA

Sequences:

>Translated_479_residues
MNSEDVKEITRVECDSLGSKEIPKDAYYGIQSKRAQENFPLSGYRVHPELIKAMGKVKYASVVANMKTGKMPNKSGEAIK
QACEEMSSGKFFDAIVVEAIQGGAGTSINMNVNEILANRAIEILGHEKGDYDVVSPNTHVNMSQSTNDVVPTSFKLAVIT
MREDLISKLENLYQTLSKKEQEFDKVIKTGRTHLQDAVPIRLGQEFGAYARFVKRDIERISNSIEALKTVNLGATAVGTG
LNADSKYINTAIEELREITGIDLEISDHLVDATQNTDAYVHVSGALKTAALNMSKMANDLRLMSSGPRCGFKEIKLPAVQ
PGSSIMPGKVNPVIPEVINQICFQVAGNDHTISMASEAGQLELNVMGPVLFRNMFESMEIIANGAHILSEKCIKGIEPNH
DRCNELVENSIGIITALNPHIGYETASEIAKEVLETGRSVRDVILEREILTEEEIEDILDPKEMTEPGIAGKKKNDVTA
>Mature_479_residues
MNSEDVKEITRVECDSLGSKEIPKDAYYGIQSKRAQENFPLSGYRVHPELIKAMGKVKYASVVANMKTGKMPNKSGEAIK
QACEEMSSGKFFDAIVVEAIQGGAGTSINMNVNEILANRAIEILGHEKGDYDVVSPNTHVNMSQSTNDVVPTSFKLAVIT
MREDLISKLENLYQTLSKKEQEFDKVIKTGRTHLQDAVPIRLGQEFGAYARFVKRDIERISNSIEALKTVNLGATAVGTG
LNADSKYINTAIEELREITGIDLEISDHLVDATQNTDAYVHVSGALKTAALNMSKMANDLRLMSSGPRCGFKEIKLPAVQ
PGSSIMPGKVNPVIPEVINQICFQVAGNDHTISMASEAGQLELNVMGPVLFRNMFESMEIIANGAHILSEKCIKGIEPNH
DRCNELVENSIGIITALNPHIGYETASEIAKEVLETGRSVRDVILEREILTEEEIEDILDPKEMTEPGIAGKKKNDVTA

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=40, Blast_Score=352, Evalue=6e-97,
Organism=Escherichia coli, GI87082375, Length=469, Percent_Identity=48.8272921108742, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=40.1727861771058, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI1790398, Length=304, Percent_Identity=25, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=41.0869565217391, Blast_Score=355, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=40.0611620795107, Blast_Score=256, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=41.304347826087, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=39.0455531453362, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=39.0455531453362, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=39.7408207343413, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=38.7445887445887, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=37.9310344827586, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52436; Mature: 52436

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSEDVKEITRVECDSLGSKEIPKDAYYGIQSKRAQENFPLSGYRVHPELIKAMGKVKYA
CCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHCCCCCCCCEEECHHHHHHHHHHHHH
SVVANMKTGKMPNKSGEAIKQACEEMSSGKFFDAIVVEAIQGGAGTSINMNVNEILANRA
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCHHHHHHHHH
IEILGHEKGDYDVVSPNTHVNMSQSTNDVVPTSFKLAVITMREDLISKLENLYQTLSKKE
HHHHCCCCCCCEEECCCCCEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
QEFDKVIKTGRTHLQDAVPIRLGQEFGAYARFVKRDIERISNSIEALKTVNLGATAVGTG
HHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
LNADSKYINTAIEELREITGIDLEISDHLVDATQNTDAYVHVSGALKTAALNMSKMANDL
CCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHH
RLMSSGPRCGFKEIKLPAVQPGSSIMPGKVNPVIPEVINQICFQVAGNDHTISMASEAGQ
HHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCC
LELNVMGPVLFRNMFESMEIIANGAHILSEKCIKGIEPNHDRCNELVENSIGIITALNPH
EEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCC
IGYETASEIAKEVLETGRSVRDVILEREILTEEEIEDILDPKEMTEPGIAGKKKNDVTA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSEDVKEITRVECDSLGSKEIPKDAYYGIQSKRAQENFPLSGYRVHPELIKAMGKVKYA
CCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHCCCCCCCCEEECHHHHHHHHHHHHH
SVVANMKTGKMPNKSGEAIKQACEEMSSGKFFDAIVVEAIQGGAGTSINMNVNEILANRA
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCHHHHHHHHH
IEILGHEKGDYDVVSPNTHVNMSQSTNDVVPTSFKLAVITMREDLISKLENLYQTLSKKE
HHHHCCCCCCCEEECCCCCEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
QEFDKVIKTGRTHLQDAVPIRLGQEFGAYARFVKRDIERISNSIEALKTVNLGATAVGTG
HHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
LNADSKYINTAIEELREITGIDLEISDHLVDATQNTDAYVHVSGALKTAALNMSKMANDL
CCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHH
RLMSSGPRCGFKEIKLPAVQPGSSIMPGKVNPVIPEVINQICFQVAGNDHTISMASEAGQ
HHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCC
LELNVMGPVLFRNMFESMEIIANGAHILSEKCIKGIEPNHDRCNELVENSIGIITALNPH
EEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCC
IGYETASEIAKEVLETGRSVRDVILEREILTEEEIEDILDPKEMTEPGIAGKKKNDVTA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]