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The map label for this gene is mleN [H]

Identifier: 188587249

GI number: 188587249

Start: 2852071

End: 2853465

Strand: Reverse

Name: mleN [H]

Synonym: Nther_2651

Alternate gene names: 188587249

Gene position: 2853465-2852071 (Counterclockwise)

Preceding gene: 188587250

Following gene: 188587248

Centisome position: 90.14

GC content: 39.71

Gene sequence:

>1395_bases
ATGGGTGAAGTTAGAAAACCAAGTATTGGTGAGGTTTTAGTCGTCCTGATAGCGTTTTTAGTTGTAGTATTTTCTTTTGT
GGCAATTTATGATTTACCAATTCAACTGGCTTTATTTATTTCATGGTTCATAGTTATCGCCCTGGGATTGCGTTTAGGTT
ACAGTTATGCGGATTTGGAAGATGGCATCAAAGATGGAATTCGTGAGGGCTTGGGAGCAATTTTCATTTTAATTGCTGTA
GGAGCTTTAATCGGTACCTGGATATCCGGTGGTATTATTGGTACATTGATCTATTATGGTTTGGCCATTATCCATCCCAG
TATTTTCTTACTGGCAACCTTGATAATCACTTCCTTGACTTCCCTGGCAACAGGGACATCCTGGGGTTCAGCAGGAACTT
CAGGGATTGCAATGATGGGTATTGGGGCAGGTTTTGGCTATCCATTACCCCTAGTAGCTGGTGCAGTGTTATCTGGTGTA
TATTTTGGTGACAAACTTTCCCCTCTATCTGATAGTACTAATCTGACTGCTTCTATGTGTAAAATTGATGTGATTGAACA
CGTTCGTTCAATGATTTACTTGATAGTTCCTGCCTGGGTAATTTCAGGAATTTTGTATACTGTAGTTGGTTTCACTATGG
ATGTAGGAACGGCACCAGAGGAAATCGCCAGGGTAGAGGAATATATGCAAGTGATGCAGGAATATTTCGTTGTTAATCCT
GCTATGTTGATTCCAGCTGTAGTTGTAATTGCTTTATTAATACTTCGTAAACCACCAGTTGCCAGTATAGCTTTTGGTTC
ATTATTAGGAGTAATTTGGGCAACTTTATTTCAAGGGATGAACTTTTTTGAAGCCTTGGGAACTGCCTATGATGGATTCG
CCATTGATACAGGAGTTGAATTCATTGATGATCTGTTAAGTCGTGGTGGAATATATGAAATGTTATCTTCCATTGCAGTG
ATTATGTTTGGACTTGGTTTTGGAGGCCTTTTAGAAAAGATAGGAGCTTTGAGGGTCATAGTGGAAAAAGTTGCTTTCTT
GTTCTCCAATCAGGGAAGAGCTTCTATAGGTTCAATCATCGTCGGTGTTATGGGTAACATGTTTGGTTCTGCCATGTATG
TATCTCTAATAGTACCTCCTAAAATCATGCAAGGAAGTTATGACGAATTGAAACTAAAACGAACAGTATTATCCCGCAAC
TCAGAAGTGGGAGGTACTTTAACTTCAGCCATGGTCCCATGGTCCGACAATGGTATCTTCATGGCTACATTGCTGGGTGT
GTCCGTTTTTGAGTATATTCCATTTATGTGGTTGGCCTTAATCTCAATTCTCCTTTCAATAATTTACGGCTACACCGGTA
TGTTTATGTGGAAGACTGAAGAAGCTGACAATTAA

Upstream 100 bases:

>100_bases
TCCTACTCGATTGTTTAAAACTTAATGAATCTTAAAACTTAATGAATGGATTCAATAGATAATTAATATACATCTAATAC
ACCATAAAGGGGGGATTTTA

Downstream 100 bases:

>100_bases
TTGACGTACTCCGCCTACAGTTGAAGCAATGGTTTAGCTACAGTAAATTGTAAAGGAGCGGATGCTATGAAAAAGGTATT
GCTGTTATCAACAGGAGGTA

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MGEVRKPSIGEVLVVLIAFLVVVFSFVAIYDLPIQLALFISWFIVIALGLRLGYSYADLEDGIKDGIREGLGAIFILIAV
GALIGTWISGGIIGTLIYYGLAIIHPSIFLLATLIITSLTSLATGTSWGSAGTSGIAMMGIGAGFGYPLPLVAGAVLSGV
YFGDKLSPLSDSTNLTASMCKIDVIEHVRSMIYLIVPAWVISGILYTVVGFTMDVGTAPEEIARVEEYMQVMQEYFVVNP
AMLIPAVVVIALLILRKPPVASIAFGSLLGVIWATLFQGMNFFEALGTAYDGFAIDTGVEFIDDLLSRGGIYEMLSSIAV
IMFGLGFGGLLEKIGALRVIVEKVAFLFSNQGRASIGSIIVGVMGNMFGSAMYVSLIVPPKIMQGSYDELKLKRTVLSRN
SEVGGTLTSAMVPWSDNGIFMATLLGVSVFEYIPFMWLALISILLSIIYGYTGMFMWKTEEADN

Sequences:

>Translated_464_residues
MGEVRKPSIGEVLVVLIAFLVVVFSFVAIYDLPIQLALFISWFIVIALGLRLGYSYADLEDGIKDGIREGLGAIFILIAV
GALIGTWISGGIIGTLIYYGLAIIHPSIFLLATLIITSLTSLATGTSWGSAGTSGIAMMGIGAGFGYPLPLVAGAVLSGV
YFGDKLSPLSDSTNLTASMCKIDVIEHVRSMIYLIVPAWVISGILYTVVGFTMDVGTAPEEIARVEEYMQVMQEYFVVNP
AMLIPAVVVIALLILRKPPVASIAFGSLLGVIWATLFQGMNFFEALGTAYDGFAIDTGVEFIDDLLSRGGIYEMLSSIAV
IMFGLGFGGLLEKIGALRVIVEKVAFLFSNQGRASIGSIIVGVMGNMFGSAMYVSLIVPPKIMQGSYDELKLKRTVLSRN
SEVGGTLTSAMVPWSDNGIFMATLLGVSVFEYIPFMWLALISILLSIIYGYTGMFMWKTEEADN
>Mature_463_residues
GEVRKPSIGEVLVVLIAFLVVVFSFVAIYDLPIQLALFISWFIVIALGLRLGYSYADLEDGIKDGIREGLGAIFILIAVG
ALIGTWISGGIIGTLIYYGLAIIHPSIFLLATLIITSLTSLATGTSWGSAGTSGIAMMGIGAGFGYPLPLVAGAVLSGVY
FGDKLSPLSDSTNLTASMCKIDVIEHVRSMIYLIVPAWVISGILYTVVGFTMDVGTAPEEIARVEEYMQVMQEYFVVNPA
MLIPAVVVIALLILRKPPVASIAFGSLLGVIWATLFQGMNFFEALGTAYDGFAIDTGVEFIDDLLSRGGIYEMLSSIAVI
MFGLGFGGLLEKIGALRVIVEKVAFLFSNQGRASIGSIIVGVMGNMFGSAMYVSLIVPPKIMQGSYDELKLKRTVLSRNS
EVGGTLTSAMVPWSDNGIFMATLLGVSVFEYIPFMWLALISILLSIIYGYTGMFMWKTEEADN

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 49758; Mature: 49627

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure 
GEVRKPSIGEVLVVLIAFLVVVFSFVAIYDLPIQLALFISWFIVIALGLRLGYSYADLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]