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The map label for this gene is ansA [H]

Identifier: 188587248

GI number: 188587248

Start: 2850997

End: 2852004

Strand: Reverse

Name: ansA [H]

Synonym: Nther_2650

Alternate gene names: 188587248

Gene position: 2852004-2850997 (Counterclockwise)

Preceding gene: 188587249

Following gene: 188587247

Centisome position: 90.09

GC content: 38.29

Gene sequence:

>1008_bases
ATGAAAAAGGTATTGCTGTTATCAACAGGAGGTACTATTGCTTCTATAAAAACGGAAAAAGGGCTAACACCGGGTCGTTT
TTCCCGTGAACTGCTCGAGTATATTCCGCAAGTTAAGTACTTTGCAGAAGTGACTGCCAAAGATTTGCTGAATATTGACA
GCTCCAATATGCAACCTGAAGATTGGCTTGAAATCGCTAAAGAAATAGTCCGAAATTATAATGATTATGATGGATTTGTC
ATCACTCACGGAACTGATACCATGGAATACACATCAAGTGCCCTGACTTATTTATTGAAAAATCTTTCTAAACCTGTAGT
TTTAACAGGTTCCCAGAAATCCATAGAAGAACCTTTGGGAGATGCCAATAGAAATTTGATAGATGCTGTTAGATTCGCTT
GCGAAGATATTTCCGGGGTTTTTGTAGCCTTTTGCGGAAAAATCATCAATGGCCCCAGAGCTAAAAAGATTAACAGTAAA
AGTTATGATGCCTTTGACAGTATAAATTATCCTGTAGTGGCGTCAGTTAAAGAAAATTATGTCTGTTATCATGAAAGAGC
CTGCCAGGATTTTGAGCCAAGTTTATCTAGTTATAATGGTCAAGTATATTGTGAAGATCAGATAGTCCCAGATGTGAATT
TACTAAAATTAGTACCTGGATTAGATCCTGGGCTGATTAAGGAGATATCCAAACATTGCAGGGGAATGGTGATTGAAAGC
TTTGGACTAGGGGGCTTGTCCGATCGCAAAAGAAACATGTTCGATGCTCTAGAAAATGCTCTCAATAGAGGTATCTACAT
TGCCATAACCACTCAAGTTTTACGGGAAGGGATTGAGCTGGGGACTTATGCATTGGGAGACAAAGTCACTAATCTAAAGG
TTATTTCAGGTAAAGACATGACTCCCAGTGCCTTGGTGGTCAAAATGATGTGGGCTTTGGCTAAGAACCAAAACTTTGAG
GAATTAAAAAAAGTGATTCATACTCCCATTCAAAAGGATATTTATTAA

Upstream 100 bases:

>100_bases
GTTTATGTGGAAGACTGAAGAAGCTGACAATTAATTGACGTACTCCGCCTACAGTTGAAGCAATGGTTTAGCTACAGTAA
ATTGTAAAGGAGCGGATGCT

Downstream 100 bases:

>100_bases
GAGTTGGCTGTACAAAAGTTGGCTCTAAATGTTATAGAAAAGAAAATGTACCGGTACTTCATTTAATGGAGTACCGGTTT
TTTAAATCTAAAGTTAATTT

Product: L-asparaginase, type I

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKKVLLLSTGGTIASIKTEKGLTPGRFSRELLEYIPQVKYFAEVTAKDLLNIDSSNMQPEDWLEIAKEIVRNYNDYDGFV
ITHGTDTMEYTSSALTYLLKNLSKPVVLTGSQKSIEEPLGDANRNLIDAVRFACEDISGVFVAFCGKIINGPRAKKINSK
SYDAFDSINYPVVASVKENYVCYHERACQDFEPSLSSYNGQVYCEDQIVPDVNLLKLVPGLDPGLIKEISKHCRGMVIES
FGLGGLSDRKRNMFDALENALNRGIYIAITTQVLREGIELGTYALGDKVTNLKVISGKDMTPSALVVKMMWALAKNQNFE
ELKKVIHTPIQKDIY

Sequences:

>Translated_335_residues
MKKVLLLSTGGTIASIKTEKGLTPGRFSRELLEYIPQVKYFAEVTAKDLLNIDSSNMQPEDWLEIAKEIVRNYNDYDGFV
ITHGTDTMEYTSSALTYLLKNLSKPVVLTGSQKSIEEPLGDANRNLIDAVRFACEDISGVFVAFCGKIINGPRAKKINSK
SYDAFDSINYPVVASVKENYVCYHERACQDFEPSLSSYNGQVYCEDQIVPDVNLLKLVPGLDPGLIKEISKHCRGMVIES
FGLGGLSDRKRNMFDALENALNRGIYIAITTQVLREGIELGTYALGDKVTNLKVISGKDMTPSALVVKMMWALAKNQNFE
ELKKVIHTPIQKDIY
>Mature_335_residues
MKKVLLLSTGGTIASIKTEKGLTPGRFSRELLEYIPQVKYFAEVTAKDLLNIDSSNMQPEDWLEIAKEIVRNYNDYDGFV
ITHGTDTMEYTSSALTYLLKNLSKPVVLTGSQKSIEEPLGDANRNLIDAVRFACEDISGVFVAFCGKIINGPRAKKINSK
SYDAFDSINYPVVASVKENYVCYHERACQDFEPSLSSYNGQVYCEDQIVPDVNLLKLVPGLDPGLIKEISKHCRGMVIES
FGLGGLSDRKRNMFDALENALNRGIYIAITTQVLREGIELGTYALGDKVTNLKVISGKDMTPSALVVKMMWALAKNQNFE
ELKKVIHTPIQKDIY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=343, Percent_Identity=29.7376093294461, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1788065, Length=333, Percent_Identity=31.2312312312312, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789327, Length=176, Percent_Identity=32.9545454545455, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI193207372, Length=348, Percent_Identity=31.0344827586207, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17558280, Length=348, Percent_Identity=31.0344827586207, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI193207374, Length=348, Percent_Identity=31.0344827586207, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17558278, Length=348, Percent_Identity=31.0344827586207, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320527, Length=338, Percent_Identity=23.3727810650888, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=261, Percent_Identity=26.8199233716475, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323187, Length=261, Percent_Identity=26.8199233716475, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323186, Length=261, Percent_Identity=26.8199233716475, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323184, Length=261, Percent_Identity=26.8199233716475, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24645530, Length=344, Percent_Identity=31.9767441860465, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24639609, Length=284, Percent_Identity=33.8028169014084, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37345; Mature: 37345

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLLLSTGGTIASIKTEKGLTPGRFSRELLEYIPQVKYFAEVTAKDLLNIDSSNMQPE
CCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DWLEIAKEIVRNYNDYDGFVITHGTDTMEYTSSALTYLLKNLSKPVVLTGSQKSIEEPLG
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCC
DANRNLIDAVRFACEDISGVFVAFCGKIINGPRAKKINSKSYDAFDSINYPVVASVKENY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCCCE
VCYHERACQDFEPSLSSYNGQVYCEDQIVPDVNLLKLVPGLDPGLIKEISKHCRGMVIES
EHHHHHHCCCCCCHHHCCCCEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCEEHHH
FGLGGLSDRKRNMFDALENALNRGIYIAITTQVLREGIELGTYALGDKVTNLKVISGKDM
CCCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCHHHHCCCCCEEEEECCCCC
TPSALVVKMMWALAKNQNFEELKKVIHTPIQKDIY
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MKKVLLLSTGGTIASIKTEKGLTPGRFSRELLEYIPQVKYFAEVTAKDLLNIDSSNMQPE
CCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
DWLEIAKEIVRNYNDYDGFVITHGTDTMEYTSSALTYLLKNLSKPVVLTGSQKSIEEPLG
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCC
DANRNLIDAVRFACEDISGVFVAFCGKIINGPRAKKINSKSYDAFDSINYPVVASVKENY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCCCE
VCYHERACQDFEPSLSSYNGQVYCEDQIVPDVNLLKLVPGLDPGLIKEISKHCRGMVIES
EHHHHHHCCCCCCHHHCCCCEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCEEHHH
FGLGGLSDRKRNMFDALENALNRGIYIAITTQVLREGIELGTYALGDKVTNLKVISGKDM
CCCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHCHHHHCCCCCEEEEECCCCC
TPSALVVKMMWALAKNQNFEELKKVIHTPIQKDIY
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]