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The map label for this gene is mleN [H]

Identifier: 188587234

GI number: 188587234

Start: 2831425

End: 2832873

Strand: Reverse

Name: mleN [H]

Synonym: Nther_2636

Alternate gene names: 188587234

Gene position: 2832873-2831425 (Counterclockwise)

Preceding gene: 188587235

Following gene: 188587233

Centisome position: 89.49

GC content: 39.27

Gene sequence:

>1449_bases
TTGTCTGACAATAAGGAAAATGTACAAGAACAAGAAGAACAACAAGTTAAAATTAAACCGACACCAGCTCAAGCGTTTAC
ACCTATGATTGCACTGATTGTACTAATCTTCTTTAGTGCTATAATATTGGAAGTTGATATTCATATTCCATTATTTATCT
CCACTATCATAGCAGCTGTTGTGGCAGTTGCTTGGATGGGTCATGATTGGGAAACTGTTCAAGAAGGTATTCTTAAGGGA
ATTAACTTTGCTTTAGGTGCTGTAATAATTTTAGCTATAGTAGGTATTTTAATTGGAAGTTGGATAGAAGGTGGAGTTGT
TCCCACAATTGTTTACTACGGACTTCAGCTTTTATCCCCACAAATTTTCTTAGTGGCAACCCTGGTTATATCTGCAGTAG
TTTCAGTCTTTACTGGTAGTTCTTGGTCATCAATTGCTACTATTGGGCTGGCTCTATCAGGAATCGGTCACGGGATGGGA
ATGCCACCTGCCATGACCGTTGGTGCCATTGTTTCCGGTGCTTACTTTGGGGACAAGCTATCTCCCTTATCCGATACCAC
AGTTGTAGCCTCAAGTACAGCCGGAGTTAATATTTATGATCACATAAAACACATGCTGTACGTATCGGTACCAGCTTTTG
TGATATCTGGAATTTTGTTTGGTATTTTAGGACTTCGCTTTGCAGAAGAAGAGATGGATTATGATCAGATAGGTGAACTT
ACAGGCACTATTGCTGAACACTTTAATGTAGGTCCATTAATGTTTTTACCGATAGTGGCAGTTCTGATCATGATTTTATT
TAGAGTACCACCAATTCCTGCCTTAACAGGTGGTTGTATTCTTGGACTAATATGGGCTCTAGTATTTCAGGGTTCTGGTC
TAGCAGATGTGTTTTCTGCTATGAACTACGGGTATGTTATGGATACTGGCATAGAAATTGTTGATGAATTGTTCACTGCT
GGCGGACTCCAAAGTATGATGTGGACTATTTCGTTGATTCTAGTAGCTCTAGCCTATGGAGGGATTGTAGAGCACACCCG
AATGATGGAAGTAATGCTTGAAAAGATTTTAACAGTAGCAACTACTCACAAGAGACTATCAATTGCAGCTCACGTTTCTA
CACTGATTTTCATTTTAACTGTAAACTCTCACTATTTGACCCATGTATTGACATGTAGATCTTATAAACACGCTTTTGAA
GAAAAAGGTTTGCACCCTAAAAATGTTTCTAGAATTTCTGAAGCGTGGGGAACCCTACCTTCATCATTGATACCTTGGGG
TCCATGTGGAGCCTTTGTTTATGGGACTTTAGGGGTTTATCCATTTGACTATCTACCCTTCGCGTTTTTCATTTTAATAG
CCATGGCTCTTTCATTTATCTATGGACTTACCGGGTTCTCAATGCATAAATTAGATGATGAAGATGCTGCCGGAATAGCT
GGTGATTAA

Upstream 100 bases:

>100_bases
GTGCTTACTTTATTGTCCAATCATGACCAGTAACAAAACAAAATAATTAGTTTTTATTGATTATTGAATCTAATTAATAT
TTCATAGGAGGGATCTCTTA

Downstream 100 bases:

>100_bases
GAAGGGTTTTTTGAGCAATAGATTGAAAACTAGATTAAAAGTTAGATATTTTAAACTGAATTAAAAAGTTGAATATATTA
AGTAGTAAAAATAAAGGATT

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSDNKENVQEQEEQQVKIKPTPAQAFTPMIALIVLIFFSAIILEVDIHIPLFISTIIAAVVAVAWMGHDWETVQEGILKG
INFALGAVIILAIVGILIGSWIEGGVVPTIVYYGLQLLSPQIFLVATLVISAVVSVFTGSSWSSIATIGLALSGIGHGMG
MPPAMTVGAIVSGAYFGDKLSPLSDTTVVASSTAGVNIYDHIKHMLYVSVPAFVISGILFGILGLRFAEEEMDYDQIGEL
TGTIAEHFNVGPLMFLPIVAVLIMILFRVPPIPALTGGCILGLIWALVFQGSGLADVFSAMNYGYVMDTGIEIVDELFTA
GGLQSMMWTISLILVALAYGGIVEHTRMMEVMLEKILTVATTHKRLSIAAHVSTLIFILTVNSHYLTHVLTCRSYKHAFE
EKGLHPKNVSRISEAWGTLPSSLIPWGPCGAFVYGTLGVYPFDYLPFAFFILIAMALSFIYGLTGFSMHKLDDEDAAGIA
GD

Sequences:

>Translated_482_residues
MSDNKENVQEQEEQQVKIKPTPAQAFTPMIALIVLIFFSAIILEVDIHIPLFISTIIAAVVAVAWMGHDWETVQEGILKG
INFALGAVIILAIVGILIGSWIEGGVVPTIVYYGLQLLSPQIFLVATLVISAVVSVFTGSSWSSIATIGLALSGIGHGMG
MPPAMTVGAIVSGAYFGDKLSPLSDTTVVASSTAGVNIYDHIKHMLYVSVPAFVISGILFGILGLRFAEEEMDYDQIGEL
TGTIAEHFNVGPLMFLPIVAVLIMILFRVPPIPALTGGCILGLIWALVFQGSGLADVFSAMNYGYVMDTGIEIVDELFTA
GGLQSMMWTISLILVALAYGGIVEHTRMMEVMLEKILTVATTHKRLSIAAHVSTLIFILTVNSHYLTHVLTCRSYKHAFE
EKGLHPKNVSRISEAWGTLPSSLIPWGPCGAFVYGTLGVYPFDYLPFAFFILIAMALSFIYGLTGFSMHKLDDEDAAGIA
GD
>Mature_481_residues
SDNKENVQEQEEQQVKIKPTPAQAFTPMIALIVLIFFSAIILEVDIHIPLFISTIIAAVVAVAWMGHDWETVQEGILKGI
NFALGAVIILAIVGILIGSWIEGGVVPTIVYYGLQLLSPQIFLVATLVISAVVSVFTGSSWSSIATIGLALSGIGHGMGM
PPAMTVGAIVSGAYFGDKLSPLSDTTVVASSTAGVNIYDHIKHMLYVSVPAFVISGILFGILGLRFAEEEMDYDQIGELT
GTIAEHFNVGPLMFLPIVAVLIMILFRVPPIPALTGGCILGLIWALVFQGSGLADVFSAMNYGYVMDTGIEIVDELFTAG
GLQSMMWTISLILVALAYGGIVEHTRMMEVMLEKILTVATTHKRLSIAAHVSTLIFILTVNSHYLTHVLTCRSYKHAFEE
KGLHPKNVSRISEAWGTLPSSLIPWGPCGAFVYGTLGVYPFDYLPFAFFILIAMALSFIYGLTGFSMHKLDDEDAAGIAG
D

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 51867; Mature: 51736

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNKENVQEQEEQQVKIKPTPAQAFTPMIALIVLIFFSAIILEVDIHIPLFISTIIAAV
CCCCHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VAVAWMGHDWETVQEGILKGINFALGAVIILAIVGILIGSWIEGGVVPTIVYYGLQLLSP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
QIFLVATLVISAVVSVFTGSSWSSIATIGLALSGIGHGMGMPPAMTVGAIVSGAYFGDKL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
SPLSDTTVVASSTAGVNIYDHIKHMLYVSVPAFVISGILFGILGLRFAEEEMDYDQIGEL
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TGTIAEHFNVGPLMFLPIVAVLIMILFRVPPIPALTGGCILGLIWALVFQGSGLADVFSA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
MNYGYVMDTGIEIVDELFTAGGLQSMMWTISLILVALAYGGIVEHTRMMEVMLEKILTVA
HCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTHKRLSIAAHVSTLIFILTVNSHYLTHVLTCRSYKHAFEEKGLHPKNVSRISEAWGTLP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
SSLIPWGPCGAFVYGTLGVYPFDYLPFAFFILIAMALSFIYGLTGFSMHKLDDEDAAGIA
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
GD
CC
>Mature Secondary Structure 
SDNKENVQEQEEQQVKIKPTPAQAFTPMIALIVLIFFSAIILEVDIHIPLFISTIIAAV
CCCHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH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CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]