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The map label for this gene is fadH [C]

Identifier: 188587173

GI number: 188587173

Start: 2760991

End: 2762949

Strand: Direct

Name: fadH [C]

Synonym: Nther_2572

Alternate gene names: 188587173

Gene position: 2760991-2762949 (Clockwise)

Preceding gene: 188587169

Following gene: 188587176

Centisome position: 87.22

GC content: 38.64

Gene sequence:

>1959_bases
ATGGATAAAAAGTATTCAAAGATTTTTGAACCTATTAACATAGGTGGTGTTGAGGTTAGGAATAGAATTAATATGGCACC
AATGACTACACTTTACGCCGGACATAATGGAGAGGTTACAGAACAAATTGTACAGTATTACGGAGCCAGGGCAAGAGGTG
GCACCGGTATGGTAACTGTAGAAGGTGCATATGTAAATGAGATGGGAATTCAAATACCAGGTTCAATCAATGTAAGCGAT
GACAAATATATCCCTGGATTATCTAGGGTTGCAGATGCCATCAAGGGTAATGGCGGTGTAGCAGTTTTACAGCTCATACA
TTCCGGGATACAAGCTTGGGTGGAACAAGCAGTCGGCCCAAGTGAAATTGGCCGAATCGATGGCAAACCAATTTCTACAG
AAAAGACACCAAGGGCCCTGACTACAAAAGAAGTTAACCAATATGTCCAAGATTTTGCAGATGCCGCCCTTAGAGCAAAG
ATAGCAGGTTTTGATATTGTCCAAGTTCATGGAACACATGGATACCTTATTATGCAATTTTTGTCTCCATTAACCAATAA
AAGAACAGACAACTATGGAGCCGACAGAGACCTTTTTCCAGTTGAAATTGTCAAAGCCATTAAAGAAAAATGTGGACAAG
ACTTCCCGGTGATGTATAGATTATGTGCCGATGAGTCCTTGGGAGATGAACTAATCAATGGTGGTATAACTTTAGAAGAT
GCTAAAAAGACTGCTGAGAAACTTGAAAATGTTGGAGTAGATGCTTTCGACGTAACTGGAGGATCTGATGATGTGATCCA
CCTCTATGTACCATCTTCATACGTATTAGAAGAAAATGAAGGTTGTTTTATAGATTTAGCTGCAGAAATTAAAAAAACAG
TAAATGTGCCTGTAATGAGTGGCGGAGGAGTAGAAAGCCCTGATGCAGCAGAACAATTGTTAGAATCAGGTCAGGTTGAC
ATGATATTTATGGGAAGGCAACTAATTGCTGATCCAGAGTGGGTATCGAAAATAGAAAAAGAAAATATAGAAGGAATCAA
ACAATGTGTTAAATGTGTTGAATGTGGCAAAAGAATAGCCTTTCTTAGGGACATGAGATGTGCCGTCAATCCTATAGTAG
GTAATGAGTGGAAATATTTAACCGAAAAGGATCTGCCTAAGGCAGCAAATAAAAAGAAAATCCTTGTAATAGGAGCTGGG
CCCGGTGGCTTAGAAGCTGCTAGGACATCGGCCATGAGAGGACATGATGTTACAATAGTGGATAAAGAATCAAAAGTAGG
TGGAACATTAAATGTAGCTGCAGTCCCCAATTTCAAAAGTAAATTTAAAAAATTAATTAATTGGTACAAGCAGCAGCTTG
AAGAGTTAAAGATAAACCTTATATTAAATAAATCTGCAGATTTGGAATTTATCCAAGAAGTTAATCCAGATGAAGTGATA
TTAGCTACAGGTTCCGAAGAAGTAGTGCCATCTATTCCGGGAGTAGAAAATACAGTTATATCGGATGAAGTATTACTCGG
TAATAAAGAGGTAGGGGAAAATGTTGTTGTAATAGGATGTGGACTTGTAGGTGCTGAAACCGCCTACTATATCGCAAAGT
CAGGTAAAAAAGTAAACGTTTTTGAAACACTACCACAAACAGATTTAGGCATAGGGGGGATCGCATTATTAAGACCCTCT
GGACTATTTGAAAAGTACGGTGTAACAGTTAATTTCAATACACCAATAGTCGAAATATATGAAGATGGAGTTTTGACAGT
TGATAAATTTGGCAAGAAAATAATCACAAAAGCAGATACTATTGTCTGCGCAGTAGGTAGGCAACCAGTACATGGCAGTG
ATTTTGTAAAGTCTATCCGAGAAAATGGCATGGTAGTAAAACCAGTTGGTGATGTTAAGTCTGCCAGAACGGTTTATGAA
GCTCTCCATGAAGCCTTTAATGCAGCCGTCAGTATATAA

Upstream 100 bases:

>100_bases
CTACAGTAGAGCTTTTAGATTGACAAACATACCGGGGTATGTTACTCTAGTCACATAAGACATACCTCGGTATGTTAAAT
TTCATTAGGAGGCGTTTATA

Downstream 100 bases:

>100_bases
TGATAGTCTAAGGAATGACAGTCTGGGAAATGGTAATGCTATGGAAATGATAGCCTAGAGTCTATGAAAATTGATTTTAA
TGGAGTAATCACTTAGAAAA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MDKKYSKIFEPINIGGVEVRNRINMAPMTTLYAGHNGEVTEQIVQYYGARARGGTGMVTVEGAYVNEMGIQIPGSINVSD
DKYIPGLSRVADAIKGNGGVAVLQLIHSGIQAWVEQAVGPSEIGRIDGKPISTEKTPRALTTKEVNQYVQDFADAALRAK
IAGFDIVQVHGTHGYLIMQFLSPLTNKRTDNYGADRDLFPVEIVKAIKEKCGQDFPVMYRLCADESLGDELINGGITLED
AKKTAEKLENVGVDAFDVTGGSDDVIHLYVPSSYVLEENEGCFIDLAAEIKKTVNVPVMSGGGVESPDAAEQLLESGQVD
MIFMGRQLIADPEWVSKIEKENIEGIKQCVKCVECGKRIAFLRDMRCAVNPIVGNEWKYLTEKDLPKAANKKKILVIGAG
PGGLEAARTSAMRGHDVTIVDKESKVGGTLNVAAVPNFKSKFKKLINWYKQQLEELKINLILNKSADLEFIQEVNPDEVI
LATGSEEVVPSIPGVENTVISDEVLLGNKEVGENVVVIGCGLVGAETAYYIAKSGKKVNVFETLPQTDLGIGGIALLRPS
GLFEKYGVTVNFNTPIVEIYEDGVLTVDKFGKKIITKADTIVCAVGRQPVHGSDFVKSIRENGMVVKPVGDVKSARTVYE
ALHEAFNAAVSI

Sequences:

>Translated_652_residues
MDKKYSKIFEPINIGGVEVRNRINMAPMTTLYAGHNGEVTEQIVQYYGARARGGTGMVTVEGAYVNEMGIQIPGSINVSD
DKYIPGLSRVADAIKGNGGVAVLQLIHSGIQAWVEQAVGPSEIGRIDGKPISTEKTPRALTTKEVNQYVQDFADAALRAK
IAGFDIVQVHGTHGYLIMQFLSPLTNKRTDNYGADRDLFPVEIVKAIKEKCGQDFPVMYRLCADESLGDELINGGITLED
AKKTAEKLENVGVDAFDVTGGSDDVIHLYVPSSYVLEENEGCFIDLAAEIKKTVNVPVMSGGGVESPDAAEQLLESGQVD
MIFMGRQLIADPEWVSKIEKENIEGIKQCVKCVECGKRIAFLRDMRCAVNPIVGNEWKYLTEKDLPKAANKKKILVIGAG
PGGLEAARTSAMRGHDVTIVDKESKVGGTLNVAAVPNFKSKFKKLINWYKQQLEELKINLILNKSADLEFIQEVNPDEVI
LATGSEEVVPSIPGVENTVISDEVLLGNKEVGENVVVIGCGLVGAETAYYIAKSGKKVNVFETLPQTDLGIGGIALLRPS
GLFEKYGVTVNFNTPIVEIYEDGVLTVDKFGKKIITKADTIVCAVGRQPVHGSDFVKSIRENGMVVKPVGDVKSARTVYE
ALHEAFNAAVSI
>Mature_652_residues
MDKKYSKIFEPINIGGVEVRNRINMAPMTTLYAGHNGEVTEQIVQYYGARARGGTGMVTVEGAYVNEMGIQIPGSINVSD
DKYIPGLSRVADAIKGNGGVAVLQLIHSGIQAWVEQAVGPSEIGRIDGKPISTEKTPRALTTKEVNQYVQDFADAALRAK
IAGFDIVQVHGTHGYLIMQFLSPLTNKRTDNYGADRDLFPVEIVKAIKEKCGQDFPVMYRLCADESLGDELINGGITLED
AKKTAEKLENVGVDAFDVTGGSDDVIHLYVPSSYVLEENEGCFIDLAAEIKKTVNVPVMSGGGVESPDAAEQLLESGQVD
MIFMGRQLIADPEWVSKIEKENIEGIKQCVKCVECGKRIAFLRDMRCAVNPIVGNEWKYLTEKDLPKAANKKKILVIGAG
PGGLEAARTSAMRGHDVTIVDKESKVGGTLNVAAVPNFKSKFKKLINWYKQQLEELKINLILNKSADLEFIQEVNPDEVI
LATGSEEVVPSIPGVENTVISDEVLLGNKEVGENVVVIGCGLVGAETAYYIAKSGKKVNVFETLPQTDLGIGGIALLRPS
GLFEKYGVTVNFNTPIVEIYEDGVLTVDKFGKKIITKADTIVCAVGRQPVHGSDFVKSIRENGMVVKPVGDVKSARTVYE
ALHEAFNAAVSI

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=542, Percent_Identity=30.4428044280443, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1787939, Length=354, Percent_Identity=27.4011299435028, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17565138, Length=347, Percent_Identity=27.9538904899135, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17540738, Length=343, Percent_Identity=30.0291545189504, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17564188, Length=344, Percent_Identity=27.3255813953488, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI72001454, Length=344, Percent_Identity=28.7790697674419, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17559804, Length=344, Percent_Identity=26.453488372093, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=271, Percent_Identity=30.6273062730627, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17566914, Length=351, Percent_Identity=26.4957264957265, Blast_Score=94, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325086, Length=231, Percent_Identity=29.004329004329, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321973, Length=361, Percent_Identity=25.207756232687, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 70613; Mature: 70613

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKYSKIFEPINIGGVEVRNRINMAPMTTLYAGHNGEVTEQIVQYYGARARGGTGMVTV
CCCHHHHHCCCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEE
EGAYVNEMGIQIPGSINVSDDKYIPGLSRVADAIKGNGGVAVLQLIHSGIQAWVEQAVGP
CCCEEECCCEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCH
SEIGRIDGKPISTEKTPRALTTKEVNQYVQDFADAALRAKIAGFDIVQVHGTHGYLIMQF
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHH
LSPLTNKRTDNYGADRDLFPVEIVKAIKEKCGQDFPVMYRLCADESLGDELINGGITLED
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCCEEEHH
AKKTAEKLENVGVDAFDVTGGSDDVIHLYVPSSYVLEENEGCFIDLAAEIKKTVNVPVMS
HHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCEEEECCCCCEEEEHHHHHHHCCCEEEC
GGGVESPDAAEQLLESGQVDMIFMGRQLIADPEWVSKIEKENIEGIKQCVKCVECGKRIA
CCCCCCCHHHHHHHHCCCEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRDMRCAVNPIVGNEWKYLTEKDLPKAANKKKILVIGAGPGGLEAARTSAMRGHDVTIV
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEE
DKESKVGGTLNVAAVPNFKSKFKKLINWYKQQLEELKINLILNKSADLEFIQEVNPDEVI
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHCCCCCEE
LATGSEEVVPSIPGVENTVISDEVLLGNKEVGENVVVIGCGLVGAETAYYIAKSGKKVNV
EECCCCCCCCCCCCCCCCEECCCEEECCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEH
FETLPQTDLGIGGIALLRPSGLFEKYGVTVNFNTPIVEIYEDGVLTVDKFGKKIITKADT
EECCCCCCCCCCCEEEECCCCCHHHCCEEEECCCCEEEEECCCEEEHHHHHHHHHCCCCE
IVCAVGRQPVHGSDFVKSIRENGMVVKPVGDVKSARTVYEALHEAFNAAVSI
EEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKKYSKIFEPINIGGVEVRNRINMAPMTTLYAGHNGEVTEQIVQYYGARARGGTGMVTV
CCCHHHHHCCCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEE
EGAYVNEMGIQIPGSINVSDDKYIPGLSRVADAIKGNGGVAVLQLIHSGIQAWVEQAVGP
CCCEEECCCEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCH
SEIGRIDGKPISTEKTPRALTTKEVNQYVQDFADAALRAKIAGFDIVQVHGTHGYLIMQF
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHH
LSPLTNKRTDNYGADRDLFPVEIVKAIKEKCGQDFPVMYRLCADESLGDELINGGITLED
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCCEEEHH
AKKTAEKLENVGVDAFDVTGGSDDVIHLYVPSSYVLEENEGCFIDLAAEIKKTVNVPVMS
HHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCEEEECCCCCEEEEHHHHHHHCCCEEEC
GGGVESPDAAEQLLESGQVDMIFMGRQLIADPEWVSKIEKENIEGIKQCVKCVECGKRIA
CCCCCCCHHHHHHHHCCCEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRDMRCAVNPIVGNEWKYLTEKDLPKAANKKKILVIGAGPGGLEAARTSAMRGHDVTIV
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEE
DKESKVGGTLNVAAVPNFKSKFKKLINWYKQQLEELKINLILNKSADLEFIQEVNPDEVI
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHCCCCCEE
LATGSEEVVPSIPGVENTVISDEVLLGNKEVGENVVVIGCGLVGAETAYYIAKSGKKVNV
EECCCCCCCCCCCCCCCCEECCCEEECCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEH
FETLPQTDLGIGGIALLRPSGLFEKYGVTVNFNTPIVEIYEDGVLTVDKFGKKIITKADT
EECCCCCCCCCCCEEEECCCCCHHHCCEEEECCCCEEEEECCCEEEHHHHHHHHHCCCCE
IVCAVGRQPVHGSDFVKSIRENGMVVKPVGDVKSARTVYEALHEAFNAAVSI
EEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]