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The map label for this gene is pflD [H]

Identifier: 188587082

GI number: 188587082

Start: 2661735

End: 2664125

Strand: Reverse

Name: pflD [H]

Synonym: Nther_2476

Alternate gene names: 188587082

Gene position: 2664125-2661735 (Counterclockwise)

Preceding gene: 188587083

Following gene: 188587075

Centisome position: 84.16

GC content: 37.22

Gene sequence:

>2391_bases
ATGGAAAATAATAGTAGGAATTCTGAAAGCATGAGTCCCCGTGTTAAAAAACTCAGGGAACAAAGTGTCTCTAAGAATCC
TAAAGTATCGGTAGAACGCGCTATACTTGTAACCCAGGCCTACAAAAAATATGATGGCAATGTGTCAATTCCCATATTGA
GAGCTTTAACATTTAAACACATTTTAGAAAATAAGAGTATTTCTATTAATGATGGAGAATTGATTGTGGGAGAACGAGGA
CCAGCACCTCAAGAAGTACCAACTTATCCCGAACTTTGTACCCATTCCATCACTGATTTAGAGATAATTCATGACAGGGA
AAAAGTTTTTTTTGAGGTCGACGAAACTGTTAAAAAAATACAGGACAGCGATATCATTCCTTTTTGGAAAGGAAAGTCCA
TGAGAGAAAAGATATTTGATAATATGAGCCGAGAATGGCTTGACTGTTATGAAGCGGGGATCTTTACAGAATTTATGGAA
CAAAGAGCTCCTGGACACACAGTAGCTGATGGGAAAATATATCAGAAGGGTTTATTGGATTTTAGGGATGATATCGACTT
AGAACTTAAAAATATTGATGAAATAAATGATCCTCAAGCTTACGAAAAAAGTGAACAGTTAAAAGCTATGCGTGTTTGTA
TTGATGCCCTTATAGAATTTGCCCAAAGATATGCAAATATAGCTGAAGAGTTAGCACAAGAAGTAGATGATAGCCATCGA
AAAGAAGAGTTGTTAAAGATAGCAGAAATATGTAAACGTGTTCCGGCAAAAGCGCCGCGTAATTTTTGGGAAGCACTACA
ATATTACTGGTTTGTGCATCTAGGTGTGATAACGGAATTGAATACATGGGATTCCTTTTGCCCTGGTAGACTGGATCAAC
ATTTGTATCCATTTTATTTACAAGATTTAAATGCAGGAGAGTTGACAAGGGGTTCAGCTAAAGAATTGTTGCAGTGCTTT
TGGATAAAATTCAACAATCAACCAGCTCCACCTAAAGTGGGTACCACATTGAAAGAAAGCGGTACTTACACAGACTTTTG
TAATATCAATATCGGAGGAGTGACACCTGAAGGTTTTGATGGCGTCAATGATGTAAGTTACTTACTTTTAGAAGTAATAG
ATGAAATGCAGTTGGTTCAACCAAGTACCAACATACAGGTTAGTAAACAAAATCCCAACGAATTTATTAAAGAAGCCGGA
AAAATCATCAGAAAAGGGTGGGGGCAACCCTCAGTATTTAATACTGATGCAGTCATATCTGAACTATTGAATCAGGGGAA
GGACTTGGTTGATGCCAGATGTGGCGGCACTAGTGGTTGTGTAGAAGCGGGGACCTTCGGAAAAGAAAGTTATATTTTAA
CCGGATACTTTAATTTAACCAAAATACTGGAATTAACCTTGTATAGAGGCGTTGATCCCAGATCCGGAAAGCAAATTGGA
CTAAAAACAGATGATCCTGAAACCTTTGATAGTTTTCAAGATTTAATAGATGCCTTTAATGAACAATTGAATTATTTTAT
AGATATAAAAATCCGAGGGAACAACATCATAGAAAAACTATATGATAAATATATGCCGTCACCTTTTCTTTCAGTCATAA
TCGATGATTGTATCAAAAAAGCCAAAGACTATAATTCTGGTGGGGCAAGGTATAATACAAGATATATTCAAGGTGTTGGG
ATAGGGACAATTACCGATAGTTTGTCTGCCATAAAACACCACATTTTTGAAAAACAGAATTACACGTGGAATGAGCTGTT
AACAGCTTTACAAAACAACTTTGAAGGCTTTAAACAGATGAGAGGATTTTTGAAAAACAAAACACCCAGGTACGGTAATG
ACGACGACTCTGCAGATGATTTGATGAAGGAAGTTTTCCAAAGGTTCTACGATGCAGTGAATGGTCGTAAAACTATGACC
GGAGGTACTTATAGAATAAATATGCTGCCCACTACTTGCCATGTTTACTTTGGATCAGTTATCGGCGCAACCCCCGAAGG
AAGATTGAGGGGAACTCCACTTTCTGAAGGTATATCACCGGTTCAAAGCGCAGATCAGGAAGGACCTACTGCTGCCTTGA
AATCTGCCGCAAAAATGGACCATATAAAAACTGGTGGAACTTTGTTAAATCTTAAATTTACTCCTCAAGTATTACAAGGG
GAGGAAGGCCTTTTAAACTTGGTTTATTTGGTTAGGTCCTATTTCCGTATGAATGGGCATCATGTTCAGTTCAATGTTGT
AGATGCCGAAACATTAAAGGATGCTCAAAGAAATCCCGATTCATATCGGAACTTAATTGTCCGAGTGGCAGGTTACAGTG
ATTATTTCAATAACTTAAACGAAAACTTACAAAATGAGATTATTAAAAGAACAGAACATTCCATAGGTTGA

Upstream 100 bases:

>100_bases
GATTTACTAATAACTGCCAGTCAAAAGGGGAGTGATTTGCTTGAAACTCATAAACTTCTGAAACAATTTGGTTTGACTGT
AGAAATGGAGGTGTGAACCC

Downstream 100 bases:

>100_bases
TAATAAATTGGAGGTTTTAGATTTATAGGTGGAAAATAGGTTGCGGGGAAATAAGCTGATTTTAAGTAAGTCGCAGGAAT
AGTTAAAAAGTCGGAGGTTA

Product: Formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 796; Mature: 796

Protein sequence:

>796_residues
MENNSRNSESMSPRVKKLREQSVSKNPKVSVERAILVTQAYKKYDGNVSIPILRALTFKHILENKSISINDGELIVGERG
PAPQEVPTYPELCTHSITDLEIIHDREKVFFEVDETVKKIQDSDIIPFWKGKSMREKIFDNMSREWLDCYEAGIFTEFME
QRAPGHTVADGKIYQKGLLDFRDDIDLELKNIDEINDPQAYEKSEQLKAMRVCIDALIEFAQRYANIAEELAQEVDDSHR
KEELLKIAEICKRVPAKAPRNFWEALQYYWFVHLGVITELNTWDSFCPGRLDQHLYPFYLQDLNAGELTRGSAKELLQCF
WIKFNNQPAPPKVGTTLKESGTYTDFCNINIGGVTPEGFDGVNDVSYLLLEVIDEMQLVQPSTNIQVSKQNPNEFIKEAG
KIIRKGWGQPSVFNTDAVISELLNQGKDLVDARCGGTSGCVEAGTFGKESYILTGYFNLTKILELTLYRGVDPRSGKQIG
LKTDDPETFDSFQDLIDAFNEQLNYFIDIKIRGNNIIEKLYDKYMPSPFLSVIIDDCIKKAKDYNSGGARYNTRYIQGVG
IGTITDSLSAIKHHIFEKQNYTWNELLTALQNNFEGFKQMRGFLKNKTPRYGNDDDSADDLMKEVFQRFYDAVNGRKTMT
GGTYRINMLPTTCHVYFGSVIGATPEGRLRGTPLSEGISPVQSADQEGPTAALKSAAKMDHIKTGGTLLNLKFTPQVLQG
EEGLLNLVYLVRSYFRMNGHHVQFNVVDAETLKDAQRNPDSYRNLIVRVAGYSDYFNNLNENLQNEIIKRTEHSIG

Sequences:

>Translated_796_residues
MENNSRNSESMSPRVKKLREQSVSKNPKVSVERAILVTQAYKKYDGNVSIPILRALTFKHILENKSISINDGELIVGERG
PAPQEVPTYPELCTHSITDLEIIHDREKVFFEVDETVKKIQDSDIIPFWKGKSMREKIFDNMSREWLDCYEAGIFTEFME
QRAPGHTVADGKIYQKGLLDFRDDIDLELKNIDEINDPQAYEKSEQLKAMRVCIDALIEFAQRYANIAEELAQEVDDSHR
KEELLKIAEICKRVPAKAPRNFWEALQYYWFVHLGVITELNTWDSFCPGRLDQHLYPFYLQDLNAGELTRGSAKELLQCF
WIKFNNQPAPPKVGTTLKESGTYTDFCNINIGGVTPEGFDGVNDVSYLLLEVIDEMQLVQPSTNIQVSKQNPNEFIKEAG
KIIRKGWGQPSVFNTDAVISELLNQGKDLVDARCGGTSGCVEAGTFGKESYILTGYFNLTKILELTLYRGVDPRSGKQIG
LKTDDPETFDSFQDLIDAFNEQLNYFIDIKIRGNNIIEKLYDKYMPSPFLSVIIDDCIKKAKDYNSGGARYNTRYIQGVG
IGTITDSLSAIKHHIFEKQNYTWNELLTALQNNFEGFKQMRGFLKNKTPRYGNDDDSADDLMKEVFQRFYDAVNGRKTMT
GGTYRINMLPTTCHVYFGSVIGATPEGRLRGTPLSEGISPVQSADQEGPTAALKSAAKMDHIKTGGTLLNLKFTPQVLQG
EEGLLNLVYLVRSYFRMNGHHVQFNVVDAETLKDAQRNPDSYRNLIVRVAGYSDYFNNLNENLQNEIIKRTEHSIG
>Mature_796_residues
MENNSRNSESMSPRVKKLREQSVSKNPKVSVERAILVTQAYKKYDGNVSIPILRALTFKHILENKSISINDGELIVGERG
PAPQEVPTYPELCTHSITDLEIIHDREKVFFEVDETVKKIQDSDIIPFWKGKSMREKIFDNMSREWLDCYEAGIFTEFME
QRAPGHTVADGKIYQKGLLDFRDDIDLELKNIDEINDPQAYEKSEQLKAMRVCIDALIEFAQRYANIAEELAQEVDDSHR
KEELLKIAEICKRVPAKAPRNFWEALQYYWFVHLGVITELNTWDSFCPGRLDQHLYPFYLQDLNAGELTRGSAKELLQCF
WIKFNNQPAPPKVGTTLKESGTYTDFCNINIGGVTPEGFDGVNDVSYLLLEVIDEMQLVQPSTNIQVSKQNPNEFIKEAG
KIIRKGWGQPSVFNTDAVISELLNQGKDLVDARCGGTSGCVEAGTFGKESYILTGYFNLTKILELTLYRGVDPRSGKQIG
LKTDDPETFDSFQDLIDAFNEQLNYFIDIKIRGNNIIEKLYDKYMPSPFLSVIIDDCIKKAKDYNSGGARYNTRYIQGVG
IGTITDSLSAIKHHIFEKQNYTWNELLTALQNNFEGFKQMRGFLKNKTPRYGNDDDSADDLMKEVFQRFYDAVNGRKTMT
GGTYRINMLPTTCHVYFGSVIGATPEGRLRGTPLSEGISPVQSADQEGPTAALKSAAKMDHIKTGGTLLNLKFTPQVLQG
EEGLLNLVYLVRSYFRMNGHHVQFNVVDAETLKDAQRNPDSYRNLIVRVAGYSDYFNNLNENLQNEIIKRTEHSIG

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=794, Percent_Identity=32.8715365239295, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1787044, Length=810, Percent_Identity=31.7283950617284, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI48994926, Length=683, Percent_Identity=25.6222547584187, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1787131, Length=686, Percent_Identity=24.4897959183673, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90384; Mature: 90384

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNSRNSESMSPRVKKLREQSVSKNPKVSVERAILVTQAYKKYDGNVSIPILRALTFKH
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILENKSISINDGELIVGERGPAPQEVPTYPELCTHSITDLEIIHDREKVFFEVDETVKKI
HHCCCCEEECCCCEEEECCCCCCCCCCCCHHHHHCCCCCHHHEECCHHHEEEHHHHHHHH
QDSDIIPFWKGKSMREKIFDNMSREWLDCYEAGIFTEFMEQRAPGHTVADGKIYQKGLLD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
FRDDIDLELKNIDEINDPQAYEKSEQLKAMRVCIDALIEFAQRYANIAEELAQEVDDSHR
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEELLKIAEICKRVPAKAPRNFWEALQYYWFVHLGVITELNTWDSFCPGRLDQHLYPFYL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHH
QDLNAGELTRGSAKELLQCFWIKFNNQPAPPKVGTTLKESGTYTDFCNINIGGVTPEGFD
HCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCCHHCCCCEEEEEEEEECCCCCCCCC
GVNDVSYLLLEVIDEMQLVQPSTNIQVSKQNPNEFIKEAGKIIRKGWGQPSVFNTDAVIS
CHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ELLNQGKDLVDARCGGTSGCVEAGTFGKESYILTGYFNLTKILELTLYRGVDPRSGKQIG
HHHHCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCEEC
LKTDDPETFDSFQDLIDAFNEQLNYFIDIKIRGNNIIEKLYDKYMPSPFLSVIIDDCIKK
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AKDYNSGGARYNTRYIQGVGIGTITDSLSAIKHHIFEKQNYTWNELLTALQNNFEGFKQM
HHCCCCCCCEEECEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RGFLKNKTPRYGNDDDSADDLMKEVFQRFYDAVNGRKTMTGGTYRINMLPTTCHVYFGSV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECHHHHHHHHHH
IGATPEGRLRGTPLSEGISPVQSADQEGPTAALKSAAKMDHIKTGGTLLNLKFTPQVLQG
HCCCCCCCCCCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHCC
EEGLLNLVYLVRSYFRMNGHHVQFNVVDAETLKDAQRNPDSYRNLIVRVAGYSDYFNNLN
CHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
ENLQNEIIKRTEHSIG
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENNSRNSESMSPRVKKLREQSVSKNPKVSVERAILVTQAYKKYDGNVSIPILRALTFKH
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILENKSISINDGELIVGERGPAPQEVPTYPELCTHSITDLEIIHDREKVFFEVDETVKKI
HHCCCCEEECCCCEEEECCCCCCCCCCCCHHHHHCCCCCHHHEECCHHHEEEHHHHHHHH
QDSDIIPFWKGKSMREKIFDNMSREWLDCYEAGIFTEFMEQRAPGHTVADGKIYQKGLLD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
FRDDIDLELKNIDEINDPQAYEKSEQLKAMRVCIDALIEFAQRYANIAEELAQEVDDSHR
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEELLKIAEICKRVPAKAPRNFWEALQYYWFVHLGVITELNTWDSFCPGRLDQHLYPFYL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHH
QDLNAGELTRGSAKELLQCFWIKFNNQPAPPKVGTTLKESGTYTDFCNINIGGVTPEGFD
HCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCCHHCCCCEEEEEEEEECCCCCCCCC
GVNDVSYLLLEVIDEMQLVQPSTNIQVSKQNPNEFIKEAGKIIRKGWGQPSVFNTDAVIS
CHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ELLNQGKDLVDARCGGTSGCVEAGTFGKESYILTGYFNLTKILELTLYRGVDPRSGKQIG
HHHHCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCEEC
LKTDDPETFDSFQDLIDAFNEQLNYFIDIKIRGNNIIEKLYDKYMPSPFLSVIIDDCIKK
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AKDYNSGGARYNTRYIQGVGIGTITDSLSAIKHHIFEKQNYTWNELLTALQNNFEGFKQM
HHCCCCCCCEEECEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RGFLKNKTPRYGNDDDSADDLMKEVFQRFYDAVNGRKTMTGGTYRINMLPTTCHVYFGSV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECHHHHHHHHHH
IGATPEGRLRGTPLSEGISPVQSADQEGPTAALKSAAKMDHIKTGGTLLNLKFTPQVLQG
HCCCCCCCCCCCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHCC
EEGLLNLVYLVRSYFRMNGHHVQFNVVDAETLKDAQRNPDSYRNLIVRVAGYSDYFNNLN
CHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
ENLQNEIIKRTEHSIG
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]