Click here to switch to the map view.

The map label for this gene is wcaL [C]

Identifier: 188586991

GI number: 188586991

Start: 2545357

End: 2546553

Strand: Reverse

Name: wcaL [C]

Synonym: Nther_2383

Alternate gene names: 188586991

Gene position: 2546553-2545357 (Counterclockwise)

Preceding gene: 188586992

Following gene: 188586990

Centisome position: 80.45

GC content: 41.77

Gene sequence:

>1197_bases
ATGAAAGTAATTCATTTAATCAGCGGGAGCGAAGGAGGCGGTTCACGATTTCAGGTATTAAGCCTCCTGGAAGAACTTGT
TCAAAATACAGATGATGACCATGATATCGAATTGGTATCTTTAATGGAAGGGCCTTTAACTCAAGACGCACGGGAACGTG
GTCTTCCCATCAAAGTTATTCCCATGAAAGGAATGTTGGATTTTAGAGTTATCTCTCCCTTGATTAGATACCTGGCGGAA
AGGAAGCCAGATATACTCCATACTCATGGGGTGAGAGCAAACTTTATTGGCAGATTGACTTACAAATTTATGTTATCTGA
TCTAAAACCCGTAATGTTTACTACAGTTCATTCGTCTATTTATCATGATTACACAAACAGCTGGAAAAGATTTATTTATC
CTTTTATGGAAAAGAGCTTGCGAGCAGTTGTTCATCGTTTTATAGCAGTATCTGATGGTTTGTATCAGGAGCTTTTGCAG
GACGGTATTGAAGAAGACAGATTGGCTTTGGTGCCAAATGGGATCTACACAGAGAAATTTTCACCTGATACTAACTCCGA
CAGTGACCCTACTACCTTGAAGGAAGAACTGGGGATTCCGGAAGAGGCGACGGTAATTTTGACTGTAGGTCGGCTTGTTC
CGGTTAAGGGTCAAGATTATCTCCTGGAAGCTTTTAAAGACTTATTGGAAGATTTGACAGAAGAAGAGGATATTGGTACT
TACTCACAAGAAAAATTGCCTTATCTTGTCATCGTAGGAGATGGTCCCCTAGGTGATAGCTTATCTTCTAAGGCCAAGAG
CTTGGGGATTGAAGAAAAAGTTATCTTTACTGGTTTTCGGCGGGATATTCCTGCTTTCTTCCAAATGGCTGATATATTTA
CCCTGCCATCTTTAATGGAGGGTATGCCTATCATTTTATTGGAAGCTATGGCAGCAAGGTTACCATTGGTGGCTAGTCGG
GTAGGGGGAGTATCTGAAGTGGTTAATGAAGGCGAAACTGGCCTCATGGTACCATCAAAAGACCCGAAAACACTAGCGGA
AGCTTTAAAGAGACTGTGGCAATCCCCGGATTTATGCCGTAAATTGGGTGGCCAAGCTGGTGAGAGAGTTGAGCGAGACC
ATCATTTTTCTCGGGTTGTCACTGAAACTTTGAAATTATATCAAAAGACTGTTAACTCAAAAAGGGAACCCGGATGA

Upstream 100 bases:

>100_bases
CTGCCAGTATTTTTGAAGTCCTGGTGGTAGGAACTTACTTTTGGATTCTGGTAGGTGTACTTCATGCTCTACCTCTGCTT
TACGAAAGGGAGGGACAGGC

Downstream 100 bases:

>100_bases
TTGGATGATTAACGGGTATGAGGAAGGAGTCGGTTGGGCATGAGTCGTGAAAATAAGAAAAATAACAACAGCCAGGAAAC
AGTTTTGATAACAGGAGGAG

Product: glycosyl transferase group 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKVIHLISGSEGGGSRFQVLSLLEELVQNTDDDHDIELVSLMEGPLTQDARERGLPIKVIPMKGMLDFRVISPLIRYLAE
RKPDILHTHGVRANFIGRLTYKFMLSDLKPVMFTTVHSSIYHDYTNSWKRFIYPFMEKSLRAVVHRFIAVSDGLYQELLQ
DGIEEDRLALVPNGIYTEKFSPDTNSDSDPTTLKEELGIPEEATVILTVGRLVPVKGQDYLLEAFKDLLEDLTEEEDIGT
YSQEKLPYLVIVGDGPLGDSLSSKAKSLGIEEKVIFTGFRRDIPAFFQMADIFTLPSLMEGMPIILLEAMAARLPLVASR
VGGVSEVVNEGETGLMVPSKDPKTLAEALKRLWQSPDLCRKLGGQAGERVERDHHFSRVVTETLKLYQKTVNSKREPG

Sequences:

>Translated_398_residues
MKVIHLISGSEGGGSRFQVLSLLEELVQNTDDDHDIELVSLMEGPLTQDARERGLPIKVIPMKGMLDFRVISPLIRYLAE
RKPDILHTHGVRANFIGRLTYKFMLSDLKPVMFTTVHSSIYHDYTNSWKRFIYPFMEKSLRAVVHRFIAVSDGLYQELLQ
DGIEEDRLALVPNGIYTEKFSPDTNSDSDPTTLKEELGIPEEATVILTVGRLVPVKGQDYLLEAFKDLLEDLTEEEDIGT
YSQEKLPYLVIVGDGPLGDSLSSKAKSLGIEEKVIFTGFRRDIPAFFQMADIFTLPSLMEGMPIILLEAMAARLPLVASR
VGGVSEVVNEGETGLMVPSKDPKTLAEALKRLWQSPDLCRKLGGQAGERVERDHHFSRVVTETLKLYQKTVNSKREPG
>Mature_398_residues
MKVIHLISGSEGGGSRFQVLSLLEELVQNTDDDHDIELVSLMEGPLTQDARERGLPIKVIPMKGMLDFRVISPLIRYLAE
RKPDILHTHGVRANFIGRLTYKFMLSDLKPVMFTTVHSSIYHDYTNSWKRFIYPFMEKSLRAVVHRFIAVSDGLYQELLQ
DGIEEDRLALVPNGIYTEKFSPDTNSDSDPTTLKEELGIPEEATVILTVGRLVPVKGQDYLLEAFKDLLEDLTEEEDIGT
YSQEKLPYLVIVGDGPLGDSLSSKAKSLGIEEKVIFTGFRRDIPAFFQMADIFTLPSLMEGMPIILLEAMAARLPLVASR
VGGVSEVVNEGETGLMVPSKDPKTLAEALKRLWQSPDLCRKLGGQAGERVERDHHFSRVVTETLKLYQKTVNSKREPG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=251, Percent_Identity=27.0916334661355, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790061, Length=197, Percent_Identity=28.4263959390863, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532709, Length=248, Percent_Identity=26.6129032258064, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI9755344, Length=263, Percent_Identity=25.0950570342205, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 44634; Mature: 44634

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIHLISGSEGGGSRFQVLSLLEELVQNTDDDHDIELVSLMEGPLTQDARERGLPIKVI
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHCCCCEEEE
PMKGMLDFRVISPLIRYLAERKPDILHTHGVRANFIGRLTYKFMLSDLKPVMFTTVHSSI
ECCCCHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YHDYTNSWKRFIYPFMEKSLRAVVHRFIAVSDGLYQELLQDGIEEDRLALVPNGIYTEKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCCCCCCCCC
SPDTNSDSDPTTLKEELGIPEEATVILTVGRLVPVKGQDYLLEAFKDLLEDLTEEEDIGT
CCCCCCCCCCHHHHHHCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHCCHHCCCC
YSQEKLPYLVIVGDGPLGDSLSSKAKSLGIEEKVIFTGFRRDIPAFFQMADIFTLPSLME
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GMPIILLEAMAARLPLVASRVGGVSEVVNEGETGLMVPSKDPKTLAEALKRLWQSPDLCR
CCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCHHHHH
KLGGQAGERVERDHHFSRVVTETLKLYQKTVNSKREPG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVIHLISGSEGGGSRFQVLSLLEELVQNTDDDHDIELVSLMEGPLTQDARERGLPIKVI
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHCCCCEEEE
PMKGMLDFRVISPLIRYLAERKPDILHTHGVRANFIGRLTYKFMLSDLKPVMFTTVHSSI
ECCCCHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YHDYTNSWKRFIYPFMEKSLRAVVHRFIAVSDGLYQELLQDGIEEDRLALVPNGIYTEKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCCCCCCCCC
SPDTNSDSDPTTLKEELGIPEEATVILTVGRLVPVKGQDYLLEAFKDLLEDLTEEEDIGT
CCCCCCCCCCHHHHHHCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHCCHHCCCC
YSQEKLPYLVIVGDGPLGDSLSSKAKSLGIEEKVIFTGFRRDIPAFFQMADIFTLPSLME
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GMPIILLEAMAARLPLVASRVGGVSEVVNEGETGLMVPSKDPKTLAEALKRLWQSPDLCR
CCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCHHHHH
KLGGQAGERVERDHHFSRVVTETLKLYQKTVNSKREPG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]