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The map label for this gene is rfbB [C]

Identifier: 188586990

GI number: 188586990

Start: 2544373

End: 2545317

Strand: Reverse

Name: rfbB [C]

Synonym: Nther_2382

Alternate gene names: 188586990

Gene position: 2545317-2544373 (Counterclockwise)

Preceding gene: 188586991

Following gene: 188586989

Centisome position: 80.41

GC content: 42.96

Gene sequence:

>945_bases
ATGAGTCGTGAAAATAAGAAAAATAACAACAGCCAGGAAACAGTTTTGATAACAGGAGGAGCCGGATTTATCGGTTCCTA
TGTGGCTGGACTCTTAATAGATCAAGGATATCGAGTTGTGATTGTGGATGACCTATCTACGGGACAAACTGGTAATATTC
CGGAATCAGCCGCGTTTTATTCACTATGTATCACAGAAGATCTTTCAAGTATATTTCTAAAGGAAAAACCCCACTACGTC
ATTCACATGGCGGCTCAGGTAAGTGTCAGCAAATCCTTGGAAGACCCAGAGGAAGACGCCAAGATAAACCTCATGGGTGG
TTTAAACCTACTCCAAGAAGCTAGTAATAACGGAGTAGAAAAGTTCGTATATGCTTCGACAGCTGCTGTTTACGGAGACC
CCTCGGAATTACCGTTAAAGGAAGAACACGAAAAAAAGCCGTTATCGCCCTATGGGATTAATAAGTTGGCCTTTGAACAA
TATCTTGAAAGTTATCGGGTAAACCTTGGGATGGATTACACTGTTCTGAGATATGCCAATGTCTATGGGCCCCGTCAGGT
CCCTGGAGCCGATGGAGGCGTTGTGGCGGTATTTATGGATAGAATTAAAAAGGGACTTCCTTTGATAATTCATGGAGATG
GCAGCCAGACCAGGGACTTTGTATATGTTGAAGATGCAGCTAGAGCTAATCTTCTGGCCCTTGAGAGGGGAAGTGGTCAA
GTTTTTAATGTCGGTTACGGTGAAGAAACTTCCATTAGTGAACTGGTAGACAGTCTAGCTCGCATCCTGGGCAGAGAGTT
GCCCTATGAATATACAAATCGACGACCGGGCGATATATATAGAAGTGTATTTAACTCAGAAAAGGCCCGGACTAATCTAG
GATTTCAAGCACAACATTCCTTGGAGTCAGGATTAATAAAAACTGTCAAGGAAGAAGATGTTTGA

Upstream 100 bases:

>100_bases
TGTCACTGAAACTTTGAAATTATATCAAAAGACTGTTAACTCAAAAAGGGAACCCGGATGATTGGATGATTAACGGGTAT
GAGGAAGGAGTCGGTTGGGC

Downstream 100 bases:

>100_bases
TGGAAGGAGGTATCCCTTGTTGCAGTATGACAGTAATAGACATGCTCTAGGTTCTGATTTGGTTTTTAAGTGGATCTTAA
TAGGGATTTTAGTTGTTGCC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSRENKKNNNSQETVLITGGAGFIGSYVAGLLIDQGYRVVIVDDLSTGQTGNIPESAAFYSLCITEDLSSIFLKEKPHYV
IHMAAQVSVSKSLEDPEEDAKINLMGGLNLLQEASNNGVEKFVYASTAAVYGDPSELPLKEEHEKKPLSPYGINKLAFEQ
YLESYRVNLGMDYTVLRYANVYGPRQVPGADGGVVAVFMDRIKKGLPLIIHGDGSQTRDFVYVEDAARANLLALERGSGQ
VFNVGYGEETSISELVDSLARILGRELPYEYTNRRPGDIYRSVFNSEKARTNLGFQAQHSLESGLIKTVKEEDV

Sequences:

>Translated_314_residues
MSRENKKNNNSQETVLITGGAGFIGSYVAGLLIDQGYRVVIVDDLSTGQTGNIPESAAFYSLCITEDLSSIFLKEKPHYV
IHMAAQVSVSKSLEDPEEDAKINLMGGLNLLQEASNNGVEKFVYASTAAVYGDPSELPLKEEHEKKPLSPYGINKLAFEQ
YLESYRVNLGMDYTVLRYANVYGPRQVPGADGGVVAVFMDRIKKGLPLIIHGDGSQTRDFVYVEDAARANLLALERGSGQ
VFNVGYGEETSISELVDSLARILGRELPYEYTNRRPGDIYRSVFNSEKARTNLGFQAQHSLESGLIKTVKEEDV
>Mature_313_residues
SRENKKNNNSQETVLITGGAGFIGSYVAGLLIDQGYRVVIVDDLSTGQTGNIPESAAFYSLCITEDLSSIFLKEKPHYVI
HMAAQVSVSKSLEDPEEDAKINLMGGLNLLQEASNNGVEKFVYASTAAVYGDPSELPLKEEHEKKPLSPYGINKLAFEQY
LESYRVNLGMDYTVLRYANVYGPRQVPGADGGVVAVFMDRIKKGLPLIIHGDGSQTRDFVYVEDAARANLLALERGSGQV
FNVGYGEETSISELVDSLARILGRELPYEYTNRRPGDIYRSVFNSEKARTNLGFQAQHSLESGLIKTVKEEDV

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=314, Percent_Identity=30.8917197452229, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI56237023, Length=327, Percent_Identity=29.0519877675841, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI56118217, Length=327, Percent_Identity=29.0519877675841, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI189083684, Length=327, Percent_Identity=29.0519877675841, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI42516563, Length=304, Percent_Identity=29.9342105263158, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4504031, Length=284, Percent_Identity=28.169014084507, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI116268111, Length=318, Percent_Identity=26.7295597484277, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI193211614, Length=280, Percent_Identity=24.2857142857143, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI8393516, Length=280, Percent_Identity=24.2857142857143, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1788353, Length=333, Percent_Identity=30.3303303303303, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI48994969, Length=331, Percent_Identity=28.0966767371601, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1786974, Length=319, Percent_Identity=27.2727272727273, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790049, Length=258, Percent_Identity=29.0697674418605, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788366, Length=277, Percent_Identity=27.7978339350181, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788365, Length=321, Percent_Identity=23.98753894081, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=333, Percent_Identity=30.3303303303303, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI71982035, Length=331, Percent_Identity=29.9093655589124, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17568069, Length=321, Percent_Identity=30.8411214953271, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI115532424, Length=324, Percent_Identity=28.7037037037037, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17539532, Length=317, Percent_Identity=26.813880126183, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI133901790, Length=347, Percent_Identity=25.3602305475504, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=347, Percent_Identity=25.3602305475504, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=347, Percent_Identity=25.3602305475504, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=347, Percent_Identity=25.3602305475504, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=347, Percent_Identity=25.3602305475504, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=358, Percent_Identity=24.3016759776536, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=333, Percent_Identity=30.03003003003, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19923002, Length=326, Percent_Identity=28.8343558282209, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356223, Length=309, Percent_Identity=28.8025889967638, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24158427, Length=288, Percent_Identity=26.3888888888889, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24667531, Length=313, Percent_Identity=25.2396166134185, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 34562; Mature: 34431

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRENKKNNNSQETVLITGGAGFIGSYVAGLLIDQGYRVVIVDDLSTGQTGNIPESAAFY
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
SLCITEDLSSIFLKEKPHYVIHMAAQVSVSKSLEDPEEDAKINLMGGLNLLQEASNNGVE
HHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCCCCEEEEECCHHHHHHHCCCCHH
KFVYASTAAVYGDPSELPLKEEHEKKPLSPYGINKLAFEQYLESYRVNLGMDYTVLRYAN
HEEEEHHEEECCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHH
VYGPRQVPGADGGVVAVFMDRIKKGLPLIIHGDGSQTRDFVYVEDAARANLLALERGSGQ
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCEEEEECCCCC
VFNVGYGEETSISELVDSLARILGRELPYEYTNRRPGDIYRSVFNSEKARTNLGFQAQHS
EEECCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHCCHHHHCCCCCHHHHH
LESGLIKTVKEEDV
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SRENKKNNNSQETVLITGGAGFIGSYVAGLLIDQGYRVVIVDDLSTGQTGNIPESAAFY
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHH
SLCITEDLSSIFLKEKPHYVIHMAAQVSVSKSLEDPEEDAKINLMGGLNLLQEASNNGVE
HHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCCCCEEEEECCHHHHHHHCCCCHH
KFVYASTAAVYGDPSELPLKEEHEKKPLSPYGINKLAFEQYLESYRVNLGMDYTVLRYAN
HEEEEHHEEECCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHH
VYGPRQVPGADGGVVAVFMDRIKKGLPLIIHGDGSQTRDFVYVEDAARANLLALERGSGQ
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCEEEEECCCCC
VFNVGYGEETSISELVDSLARILGRELPYEYTNRRPGDIYRSVFNSEKARTNLGFQAQHS
EEECCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHCCHHHHCCCCCHHHHH
LESGLIKTVKEEDV
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]