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The map label for this gene is wecC [H]

Identifier: 188586986

GI number: 188586986

Start: 2539432

End: 2540814

Strand: Reverse

Name: wecC [H]

Synonym: Nther_2378

Alternate gene names: 188586986

Gene position: 2540814-2539432 (Counterclockwise)

Preceding gene: 188586987

Following gene: 188586985

Centisome position: 80.26

GC content: 41.87

Gene sequence:

>1383_bases
ATGAACCCAGTTAATAAAACGGATCAAAATAAAATGGAAGTAAATACTGTCGGTTTAAAGCGAAACAATACCATTGCTGT
CTTTGGCCAGGGATATGTAGGATTACCTCTGGCGCTTAGCTTTGCCATGAAAGGATTCAAGGTATTTGGTGTTGATATTA
ATCAAGCTTTGATTAAAGAGTTAAAGCAGGGGAAGACCAAGCACCTAGAGAGTTACCAGGGCAAATCAATTCAGGAAATC
TTACAGGAAGAACTGGATTCGGGAAGATATATACCAGTGGATGATTACCGTCAAGCCCTAACTCAGGCGGGGATTATCGT
AGTAACAGTAGGTGCGCCTATCAAGGATGATGGAGAAGTAAATAATAGTCCTCTGAAAGCAGTTGCTCGTGAAATAGGAC
AATCATTGAAAAAAGATGACCTGGTCCTGGTACGGAGTACAGTAGTTCCTGGTACTACTGAAAGAGTGATTCAGCCAATT
TTGGAAAGCGAAAGCGGTTTGGTGTCAGGAGAAGACTTTGATTTGGCTTACTCTTCGGAACGTATAGCTGAAGGAAAAGC
TTTTCTTGAATTCAGTGAAATGCCAACTCTGGTTGCCGGAGTCACCGACTCTGGTCGTAAGAGGGCAAGTGAATTACTGG
CTTCTTTGTTTGGTGATGGTGGTGAAATTGTTGTCTCCCCTTCTATAGAGGCGGTAGAATTGTCAAAGGTTCTAGAGAAC
TTGTCCAGGGATATTAATATAGGAATGGTAAATGAATTTGCGGGTTTATGTGAGGAACTTGATTTAGATATTCACCATAT
TATTGATTTGGCCAATACCCACAAAAGGGTCAACTTATTGCGTCCTGGACCCGGTGTGGGAGGATTTTGTATTCCAAATG
CTTACTATTATCTTTTACCCAGGGCTAGAGAAGCCGGTTTAGATTTAGACTTAAGTTCTAGTGCCCGTGAAATCAATGAT
TATCGTCCCAGGCGTGTAGTTGAGCGTTTAAAAGAGAGGTTACAGGAGCAGGGGAAATCTCTAGATCAGTGCAAAATAGC
TATTTTGGGACTGGCTATGAAAGACTATTCTAGTGACCACAGGCAAAGCCCTGCTTTAGATATTGTAGAACTATTGCTAC
ATCAAGGTGGAGAAGTAAACTGTTACGACCCAGAAGTGAGTTCTGGATTGATCGCAGGTCAAGTTAATGACCTGTGGAAG
TGTGTGCAGGGAGCCAGTGCCGTGATATTTTTGACTCGACAGGAAGAGTTTGATAATTTGGATTTTGGACAGTTAAAAGG
AAAAATGTTAGCAAATCCCCACTCACCTTTGCTGTTAGATCCCAAAAAAGCCCTGTCCAGGGAAGAAGCTACTAAAGAAG
GGATTCAATACGTAATCTTGTGA

Upstream 100 bases:

>100_bases
AGGGACTGGCAAGGAATACCGCTAAATTTACACTTGATTGTTTGACTTAATTGTTATATAAAGGATTATGAAGAAAACTT
GTCCAAAGGGAGAGACTGAT

Downstream 100 bases:

>100_bases
TGGTAACTATTTACAGAGTGACACAAAGGAAGGATATCGGTCATTAAAAGCGAATTTGTAAGTTAGTTTAGGAGTGTCTA
TATATGAGCTTTTTTAAAAA

Product: nucleotide sugar dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNPVNKTDQNKMEVNTVGLKRNNTIAVFGQGYVGLPLALSFAMKGFKVFGVDINQALIKELKQGKTKHLESYQGKSIQEI
LQEELDSGRYIPVDDYRQALTQAGIIVVTVGAPIKDDGEVNNSPLKAVAREIGQSLKKDDLVLVRSTVVPGTTERVIQPI
LESESGLVSGEDFDLAYSSERIAEGKAFLEFSEMPTLVAGVTDSGRKRASELLASLFGDGGEIVVSPSIEAVELSKVLEN
LSRDINIGMVNEFAGLCEELDLDIHHIIDLANTHKRVNLLRPGPGVGGFCIPNAYYYLLPRAREAGLDLDLSSSAREIND
YRPRRVVERLKERLQEQGKSLDQCKIAILGLAMKDYSSDHRQSPALDIVELLLHQGGEVNCYDPEVSSGLIAGQVNDLWK
CVQGASAVIFLTRQEEFDNLDFGQLKGKMLANPHSPLLLDPKKALSREEATKEGIQYVIL

Sequences:

>Translated_460_residues
MNPVNKTDQNKMEVNTVGLKRNNTIAVFGQGYVGLPLALSFAMKGFKVFGVDINQALIKELKQGKTKHLESYQGKSIQEI
LQEELDSGRYIPVDDYRQALTQAGIIVVTVGAPIKDDGEVNNSPLKAVAREIGQSLKKDDLVLVRSTVVPGTTERVIQPI
LESESGLVSGEDFDLAYSSERIAEGKAFLEFSEMPTLVAGVTDSGRKRASELLASLFGDGGEIVVSPSIEAVELSKVLEN
LSRDINIGMVNEFAGLCEELDLDIHHIIDLANTHKRVNLLRPGPGVGGFCIPNAYYYLLPRAREAGLDLDLSSSAREIND
YRPRRVVERLKERLQEQGKSLDQCKIAILGLAMKDYSSDHRQSPALDIVELLLHQGGEVNCYDPEVSSGLIAGQVNDLWK
CVQGASAVIFLTRQEEFDNLDFGQLKGKMLANPHSPLLLDPKKALSREEATKEGIQYVIL
>Mature_460_residues
MNPVNKTDQNKMEVNTVGLKRNNTIAVFGQGYVGLPLALSFAMKGFKVFGVDINQALIKELKQGKTKHLESYQGKSIQEI
LQEELDSGRYIPVDDYRQALTQAGIIVVTVGAPIKDDGEVNNSPLKAVAREIGQSLKKDDLVLVRSTVVPGTTERVIQPI
LESESGLVSGEDFDLAYSSERIAEGKAFLEFSEMPTLVAGVTDSGRKRASELLASLFGDGGEIVVSPSIEAVELSKVLEN
LSRDINIGMVNEFAGLCEELDLDIHHIIDLANTHKRVNLLRPGPGVGGFCIPNAYYYLLPRAREAGLDLDLSSSAREIND
YRPRRVVERLKERLQEQGKSLDQCKIAILGLAMKDYSSDHRQSPALDIVELLLHQGGEVNCYDPEVSSGLIAGQVNDLWK
CVQGASAVIFLTRQEEFDNLDFGQLKGKMLANPHSPLLLDPKKALSREEATKEGIQYVIL

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA) [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=450, Percent_Identity=24.2222222222222, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI296040438, Length=335, Percent_Identity=25.3731343283582, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI296040443, Length=280, Percent_Identity=26.0714285714286, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI48994968, Length=374, Percent_Identity=33.1550802139037, Blast_Score=188, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17560350, Length=436, Percent_Identity=23.6238532110092, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136908, Length=464, Percent_Identity=22.6293103448276, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 50501; Mature: 50501

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVNKTDQNKMEVNTVGLKRNNTIAVFGQGYVGLPLALSFAMKGFKVFGVDINQALIKE
CCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEEECHHHHHHHH
LKQGKTKHLESYQGKSIQEILQEELDSGRYIPVDDYRQALTQAGIIVVTVGAPIKDDGEV
HHCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCC
NNSPLKAVAREIGQSLKKDDLVLVRSTVVPGTTERVIQPILESESGLVSGEDFDLAYSSE
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCH
RIAEGKAFLEFSEMPTLVAGVTDSGRKRASELLASLFGDGGEIVVSPSIEAVELSKVLEN
HHHCCHHHEEHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHH
LSRDINIGMVNEFAGLCEELDLDIHHIIDLANTHKRVNLLRPGPGVGGFCIPNAYYYLLP
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCEECCCCEEEEEC
RAREAGLDLDLSSSAREINDYRPRRVVERLKERLQEQGKSLDQCKIAILGLAMKDYSSDH
CHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHH
RQSPALDIVELLLHQGGEVNCYDPEVSSGLIAGQVNDLWKCVQGASAVIFLTRQEEFDNL
CCCCHHHHHHHHHHCCCCEEEECCCCCCCEEECCHHHHHHHHCCCCEEEEEEECCCCCCC
DFGQLKGKMLANPHSPLLLDPKKALSREEATKEGIQYVIL
CHHHHCCCHHCCCCCCEEECHHHHHHHHHHHHHCCEEEEC
>Mature Secondary Structure
MNPVNKTDQNKMEVNTVGLKRNNTIAVFGQGYVGLPLALSFAMKGFKVFGVDINQALIKE
CCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCEEEEECHHHHHHHH
LKQGKTKHLESYQGKSIQEILQEELDSGRYIPVDDYRQALTQAGIIVVTVGAPIKDDGEV
HHCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCC
NNSPLKAVAREIGQSLKKDDLVLVRSTVVPGTTERVIQPILESESGLVSGEDFDLAYSSE
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCH
RIAEGKAFLEFSEMPTLVAGVTDSGRKRASELLASLFGDGGEIVVSPSIEAVELSKVLEN
HHHCCHHHEEHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHH
LSRDINIGMVNEFAGLCEELDLDIHHIIDLANTHKRVNLLRPGPGVGGFCIPNAYYYLLP
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCEECCCCEEEEEC
RAREAGLDLDLSSSAREINDYRPRRVVERLKERLQEQGKSLDQCKIAILGLAMKDYSSDH
CHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHH
RQSPALDIVELLLHQGGEVNCYDPEVSSGLIAGQVNDLWKCVQGASAVIFLTRQEEFDNL
CCCCHHHHHHHHHHCCCCEEEECCCCCCCEEECCHHHHHHHHCCCCEEEEEEECCCCCCC
DFGQLKGKMLANPHSPLLLDPKKALSREEATKEGIQYVIL
CHHHHCCCHHCCCCCCEEECHHHHHHHHHHHHHCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]