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The map label for this gene is metK [H]

Identifier: 188586938

GI number: 188586938

Start: 2475937

End: 2477127

Strand: Reverse

Name: metK [H]

Synonym: Nther_2330

Alternate gene names: 188586938

Gene position: 2477127-2475937 (Counterclockwise)

Preceding gene: 188586940

Following gene: 188586937

Centisome position: 78.25

GC content: 42.74

Gene sequence:

>1191_bases
ATGAGCAAAAAATTAGTGACGTCGGAATCTGTTACCGAAGGCCATCCTGATAAGATTGCCGATCAAATATCCGATGCCAT
ATTAGATGCCATGCTTGAAAAAGATCCATCCGCTAGAGTGGCTGCTGAAACTACTGTGACCACAGGTTTGGTCCTGGTTA
CAGGTGAAATCACCACAAAGTGTTATGTAGACATTCCTAAACTGGTTAGAGAAACTGTACAAAATATTGGTTATACCAGG
GCAAAGTACGGCTTTGATGGTGAAACTTGTGCTGTGCTAACTTCCATTGACGAGCAGTCCCCTGATATTGCCCAGGGAGT
AGATCAAGCTTTGGAAAAAAGGCAAGAAGAAATCACCGATGATGAAATTAATGCTATCGGTGCCGGGGATCAGGGAATGA
TGTTTGGTTATGCTACTAATGAAACTGAAGAGTACCTACCCTTACCCATTGCTTTGGCTCACAAGATTACTCGTCGTTTA
GCAGCTGTCAGAAAAGATGGAACTTTACCCTATCTACGTCCAGACGGAAAAAGTCAGGTGACAGTAGAGTACAGAAATAA
TGAACCTTACAGGGTGAATAAAGTAGTAGTATCATCCCAGCATAGTCCTAAAGTGACGGTAGACCAATTGGAACAAGAAA
TTATTGATAATGTGGTAAAAGAAATCATTCCATCTAAATTCTTAGATGAGACTACCGAATATTTAATTAATCCCACGGGG
CGTTTCGTTGTAGGAGGACCTCAGGGAGATGCCGGGGCCACTGGTAGAAAGGTGATTGTAGATACATATGGTGGTGTTGC
ACGCCACGGTGGTGGTGCATTCTCCGGCAAAGATCCTACAAAAGTTGATCGTTCTGCCGCTTACGCTGCCAGATATATAG
CCAAAAATGTAGTTGCTGCAGGCCTGGCAGATAAATGTGAACTCCAAGTAGCTTATGCCATCGGTGTTGCTAATCCTGTC
AGTGTTATGGTTGAAACTTATGGAACAGCTAAGATTCCAGAAGACCAGATCGAAGAACTAGTTAATAAACACTTTGATCT
CAGGCCCGGTGCTATTATCAGGGATTTGAATCTATTAAAACCAATATACCGGCAGGTAGCCTGTTACGGCCATATGGGAA
GAACTGATATTGAGTTGCCTTGGGAACGAACTGATAAAGCAGAAATCTTAAAAACAGAAGCCGGGATTTAA

Upstream 100 bases:

>100_bases
GTCATGGGCGGTTAATGGTCTATAAAATTGACATAAATATGTTAATTTTATAGAATAAATATAACTTTGTTGATTTTTGG
AAATCAGGAGGTGAGAAATA

Downstream 100 bases:

>100_bases
TTTTACGAAAAAGGGCTGAGCACAGTCAAAATGTGTTTCAGCCCTTTTTTGTTAATACGAAAAACTATCTTTATTTTTTC
ATAAACTTTTAAGTTCAAAA

Product: methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSKKLVTSESVTEGHPDKIADQISDAILDAMLEKDPSARVAAETTVTTGLVLVTGEITTKCYVDIPKLVRETVQNIGYTR
AKYGFDGETCAVLTSIDEQSPDIAQGVDQALEKRQEEITDDEINAIGAGDQGMMFGYATNETEEYLPLPIALAHKITRRL
AAVRKDGTLPYLRPDGKSQVTVEYRNNEPYRVNKVVVSSQHSPKVTVDQLEQEIIDNVVKEIIPSKFLDETTEYLINPTG
RFVVGGPQGDAGATGRKVIVDTYGGVARHGGGAFSGKDPTKVDRSAAYAARYIAKNVVAAGLADKCELQVAYAIGVANPV
SVMVETYGTAKIPEDQIEELVNKHFDLRPGAIIRDLNLLKPIYRQVACYGHMGRTDIELPWERTDKAEILKTEAGI

Sequences:

>Translated_396_residues
MSKKLVTSESVTEGHPDKIADQISDAILDAMLEKDPSARVAAETTVTTGLVLVTGEITTKCYVDIPKLVRETVQNIGYTR
AKYGFDGETCAVLTSIDEQSPDIAQGVDQALEKRQEEITDDEINAIGAGDQGMMFGYATNETEEYLPLPIALAHKITRRL
AAVRKDGTLPYLRPDGKSQVTVEYRNNEPYRVNKVVVSSQHSPKVTVDQLEQEIIDNVVKEIIPSKFLDETTEYLINPTG
RFVVGGPQGDAGATGRKVIVDTYGGVARHGGGAFSGKDPTKVDRSAAYAARYIAKNVVAAGLADKCELQVAYAIGVANPV
SVMVETYGTAKIPEDQIEELVNKHFDLRPGAIIRDLNLLKPIYRQVACYGHMGRTDIELPWERTDKAEILKTEAGI
>Mature_395_residues
SKKLVTSESVTEGHPDKIADQISDAILDAMLEKDPSARVAAETTVTTGLVLVTGEITTKCYVDIPKLVRETVQNIGYTRA
KYGFDGETCAVLTSIDEQSPDIAQGVDQALEKRQEEITDDEINAIGAGDQGMMFGYATNETEEYLPLPIALAHKITRRLA
AVRKDGTLPYLRPDGKSQVTVEYRNNEPYRVNKVVVSSQHSPKVTVDQLEQEIIDNVVKEIIPSKFLDETTEYLINPTGR
FVVGGPQGDAGATGRKVIVDTYGGVARHGGGAFSGKDPTKVDRSAAYAARYIAKNVVAAGLADKCELQVAYAIGVANPVS
VMVETYGTAKIPEDQIEELVNKHFDLRPGAIIRDLNLLKPIYRQVACYGHMGRTDIELPWERTDKAEILKTEAGI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=55.2356020942408, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=56.282722513089, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1789311, Length=396, Percent_Identity=59.5959595959596, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17551082, Length=392, Percent_Identity=53.8265306122449, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17538494, Length=391, Percent_Identity=53.4526854219949, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17538498, Length=391, Percent_Identity=52.9411764705882, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25145633, Length=394, Percent_Identity=53.2994923857868, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=391, Percent_Identity=49.1048593350384, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565911, Length=344, Percent_Identity=50.8720930232558, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI25145635, Length=346, Percent_Identity=51.4450867052023, Blast_Score=349, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=346, Percent_Identity=50.5780346820809, Blast_Score=330, Evalue=9e-91,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=55.421686746988, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320710, Length=396, Percent_Identity=54.040404040404, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=54.8302872062663, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI45552159, Length=389, Percent_Identity=54.2416452442159, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580529, Length=389, Percent_Identity=54.2416452442159, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580537, Length=389, Percent_Identity=54.2416452442159, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580533, Length=389, Percent_Identity=54.2416452442159, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580535, Length=389, Percent_Identity=54.2416452442159, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580531, Length=389, Percent_Identity=54.2416452442159, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=53.7275064267352, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=53.7275064267352, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=391, Percent_Identity=51.4066496163683, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580539, Length=170, Percent_Identity=46.4705882352941, Blast_Score=134, Evalue=9e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43198; Mature: 43067

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLVTSESVTEGHPDKIADQISDAILDAMLEKDPSARVAAETTVTTGLVLVTGEITTK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHEEEEEEEECCCCEE
CYVDIPKLVRETVQNIGYTRAKYGFDGETCAVLTSIDEQSPDIAQGVDQALEKRQEEITD
EEECHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
DEINAIGAGDQGMMFGYATNETEEYLPLPIALAHKITRRLAAVRKDGTLPYLRPDGKSQV
CHHHEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEE
TVEYRNNEPYRVNKVVVSSQHSPKVTVDQLEQEIIDNVVKEIIPSKFLDETTEYLINPTG
EEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RFVVGGPQGDAGATGRKVIVDTYGGVARHGGGAFSGKDPTKVDRSAAYAARYIAKNVVAA
CEEEECCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GLADKCELQVAYAIGVANPVSVMVETYGTAKIPEDQIEELVNKHFDLRPGAIIRDLNLLK
CCCCHHHEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
PIYRQVACYGHMGRTDIELPWERTDKAEILKTEAGI
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
SKKLVTSESVTEGHPDKIADQISDAILDAMLEKDPSARVAAETTVTTGLVLVTGEITTK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHEEEEEEEECCCCEE
CYVDIPKLVRETVQNIGYTRAKYGFDGETCAVLTSIDEQSPDIAQGVDQALEKRQEEITD
EEECHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
DEINAIGAGDQGMMFGYATNETEEYLPLPIALAHKITRRLAAVRKDGTLPYLRPDGKSQV
CHHHEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEE
TVEYRNNEPYRVNKVVVSSQHSPKVTVDQLEQEIIDNVVKEIIPSKFLDETTEYLINPTG
EEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RFVVGGPQGDAGATGRKVIVDTYGGVARHGGGAFSGKDPTKVDRSAAYAARYIAKNVVAA
CEEEECCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GLADKCELQVAYAIGVANPVSVMVETYGTAKIPEDQIEELVNKHFDLRPGAIIRDLNLLK
CCCCHHHEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
PIYRQVACYGHMGRTDIELPWERTDKAEILKTEAGI
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA