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The map label for this gene is appA [H]

Identifier: 188586810

GI number: 188586810

Start: 2337209

End: 2338912

Strand: Direct

Name: appA [H]

Synonym: Nther_2201

Alternate gene names: 188586810

Gene position: 2337209-2338912 (Clockwise)

Preceding gene: 188586809

Following gene: 188586830

Centisome position: 73.83

GC content: 40.26

Gene sequence:

>1704_bases
ATGGAAAATGCAGGACAATTTTTTAAGAAATTTTCAGTACTCATGGCAGTTTTAATAGTTACTACTGCGTTAGTAATCGG
ATGTGGAGACGGTGGCGATCCAGACGCATCTGATGATTTGGACCCAGATAGAAAGGTTCCAGAAGTAAGATTTGTTTCAT
CCACTGCTGATGATAACCAGATAAGAAATGAAGCAGTACAGCTAGTTGCTGACTGGTGGGAAGAAATCGGTTTAGAAGTA
GATATTCAAACCAGAGAGTTTAACTCTCTAGTCAACAGAGTATTGGCTGCCCCCGAAGATAAGGATTTCGAAGCATATAT
TCTAGGTTGGAGTGGTAGAGTATCAAGATCAGACCCTGATATGTTCCTTTATTCCTTATATCATTCCAATCAAGCTGTAG
ATGGCGGTAACAACAGTAGTGTTTTCAAAAATGAAAAGTATGATGAACTTGTTTCGAAACAAAGAGCGGAAATGGATCTA
GAAAAGCGACAAGAACTCGTCTTTGAGGCCCAGGAAGTCCTGGCAGAAGAAGTTCCTGATATCACCCTATATTACAGAGA
CGAAATTCAAGGTTATAACAATGAGCGATGGGAAGATCTACCAAGTATGGCGGGAGAAGGTATCTTTAACGAACAGTTCC
CCTATGAGGCTACTCCCAAAACAGATGATGCAGAGTTTGTAATAGCTAACTCCGCAAACTTGGATACATTTAACCCCTTT
GCGGCAGAGACAGTTTACGAATGGAAATTTTTACGACTTGTTTACGACAAATTAGTTAGATTAGATGAGAATTTTGAGCC
CCAACCCTGGGCAGCAGAAGAAATCAACGTAGTAGAAGGCGAAGACGGTGAAGTAATAGATGTAACCTTGAGAGATGATT
TACAGTTCCACGATGGAGAACCACTTGGGCCAGAAGATGTTGTCTTTACTTTCGACTACATGTTTGAAGAAGGCATACCT
TATTTCCAAGCCTTTTTAGACCCAATTGAAACAGTAGACCTCATGGACGATGATGAAACTATCAGATTTACTTTAGAAGA
AGCTTATGCACCCTTTATAACCAATACTTTAGGTCAAATTCCTATCTTACCAGAACACATCTGGGCTGATGTTATGGAAG
AAGAAAATTTAGATCATCCTTCTCAGTTCGACAATGCCGAAGCTATCGGAAGCGGTCCTTTCAAATTTGACAATTGGGAA
AGAGGCGAGTACATCAGAATTGTTAAAAACGATGATTACTTCAAAGCTGATGATATCGATGTGGAAGCTATCAGATACGA
CAAGTACAGCCATTCTGAAGGAGTTTTCGGTGCCCTGGAGAACCAACAGGCCGATGTAAACGAAAACACTTTTGACCCTG
AATACGTTCAACAAGCAGAAGATCTTGATCATTTAACAGTAGTTAGAGAACCCGATATCGGCTTTGACTTCATTGGCCTC
AATAACTACAAAGAGCCTTTTAACGATAAAGCCGTAAGACAAGCCGCTGCTCATGCCATCGATTTAGATGAGCTTGTAGA
CGTTCTCTTGTACGGTTATGGAGATCCAGCAGGTGCAGGTCAGACTATTTCAACAGGTAATGAAATGTGGAAAAATGACG
ATGTAAAAGAATATCCTTTTGATATCGATAAAGCAAGAGAAATCTTAAAAGATGCCGGCTATGAATGGGATAGTGACGGA
AGATTATATTTCCCTGAAGAGTAA

Upstream 100 bases:

>100_bases
AATTTAGGCTGTTAAGAACAGAAAGTTAACGATAGATTCAAACGTATAAAGTATTATGATTGATTATCTAATTAATTTAA
ATTTAGAGGGAGGTCAAATT

Downstream 100 bases:

>100_bases
TCCTGATACAAATTAAAACTCCTGATCCACACTAAAACTCTGGTTAACTAAAGCTCCACTTACACTAAAAAGCTCCGGGT
GATACAGCAATTTGCTGTCC

Product: extracellular solute-binding protein family 5

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MENAGQFFKKFSVLMAVLIVTTALVIGCGDGGDPDASDDLDPDRKVPEVRFVSSTADDNQIRNEAVQLVADWWEEIGLEV
DIQTREFNSLVNRVLAAPEDKDFEAYILGWSGRVSRSDPDMFLYSLYHSNQAVDGGNNSSVFKNEKYDELVSKQRAEMDL
EKRQELVFEAQEVLAEEVPDITLYYRDEIQGYNNERWEDLPSMAGEGIFNEQFPYEATPKTDDAEFVIANSANLDTFNPF
AAETVYEWKFLRLVYDKLVRLDENFEPQPWAAEEINVVEGEDGEVIDVTLRDDLQFHDGEPLGPEDVVFTFDYMFEEGIP
YFQAFLDPIETVDLMDDDETIRFTLEEAYAPFITNTLGQIPILPEHIWADVMEEENLDHPSQFDNAEAIGSGPFKFDNWE
RGEYIRIVKNDDYFKADDIDVEAIRYDKYSHSEGVFGALENQQADVNENTFDPEYVQQAEDLDHLTVVREPDIGFDFIGL
NNYKEPFNDKAVRQAAAHAIDLDELVDVLLYGYGDPAGAGQTISTGNEMWKNDDVKEYPFDIDKAREILKDAGYEWDSDG
RLYFPEE

Sequences:

>Translated_567_residues
MENAGQFFKKFSVLMAVLIVTTALVIGCGDGGDPDASDDLDPDRKVPEVRFVSSTADDNQIRNEAVQLVADWWEEIGLEV
DIQTREFNSLVNRVLAAPEDKDFEAYILGWSGRVSRSDPDMFLYSLYHSNQAVDGGNNSSVFKNEKYDELVSKQRAEMDL
EKRQELVFEAQEVLAEEVPDITLYYRDEIQGYNNERWEDLPSMAGEGIFNEQFPYEATPKTDDAEFVIANSANLDTFNPF
AAETVYEWKFLRLVYDKLVRLDENFEPQPWAAEEINVVEGEDGEVIDVTLRDDLQFHDGEPLGPEDVVFTFDYMFEEGIP
YFQAFLDPIETVDLMDDDETIRFTLEEAYAPFITNTLGQIPILPEHIWADVMEEENLDHPSQFDNAEAIGSGPFKFDNWE
RGEYIRIVKNDDYFKADDIDVEAIRYDKYSHSEGVFGALENQQADVNENTFDPEYVQQAEDLDHLTVVREPDIGFDFIGL
NNYKEPFNDKAVRQAAAHAIDLDELVDVLLYGYGDPAGAGQTISTGNEMWKNDDVKEYPFDIDKAREILKDAGYEWDSDG
RLYFPEE
>Mature_567_residues
MENAGQFFKKFSVLMAVLIVTTALVIGCGDGGDPDASDDLDPDRKVPEVRFVSSTADDNQIRNEAVQLVADWWEEIGLEV
DIQTREFNSLVNRVLAAPEDKDFEAYILGWSGRVSRSDPDMFLYSLYHSNQAVDGGNNSSVFKNEKYDELVSKQRAEMDL
EKRQELVFEAQEVLAEEVPDITLYYRDEIQGYNNERWEDLPSMAGEGIFNEQFPYEATPKTDDAEFVIANSANLDTFNPF
AAETVYEWKFLRLVYDKLVRLDENFEPQPWAAEEINVVEGEDGEVIDVTLRDDLQFHDGEPLGPEDVVFTFDYMFEEGIP
YFQAFLDPIETVDLMDDDETIRFTLEEAYAPFITNTLGQIPILPEHIWADVMEEENLDHPSQFDNAEAIGSGPFKFDNWE
RGEYIRIVKNDDYFKADDIDVEAIRYDKYSHSEGVFGALENQQADVNENTFDPEYVQQAEDLDHLTVVREPDIGFDFIGL
NNYKEPFNDKAVRQAAAHAIDLDELVDVLLYGYGDPAGAGQTISTGNEMWKNDDVKEYPFDIDKAREILKDAGYEWDSDG
RLYFPEE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=351, Percent_Identity=25.0712250712251, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787551, Length=365, Percent_Identity=26.3013698630137, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787052, Length=332, Percent_Identity=23.7951807228916, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789887, Length=315, Percent_Identity=25.7142857142857, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787762, Length=321, Percent_Identity=23.98753894081, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64903; Mature: 64903

Theoretical pI: Translated: 3.80; Mature: 3.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENAGQFFKKFSVLMAVLIVTTALVIGCGDGGDPDASDDLDPDRKVPEVRFVSSTADDNQ
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
IRNEAVQLVADWWEEIGLEVDIQTREFNSLVNRVLAAPEDKDFEAYILGWSGRVSRSDPD
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCC
MFLYSLYHSNQAVDGGNNSSVFKNEKYDELVSKQRAEMDLEKRQELVFEAQEVLAEEVPD
CEEEEEHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
ITLYYRDEIQGYNNERWEDLPSMAGEGIFNEQFPYEATPKTDDAEFVIANSANLDTFNPF
EEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCH
AAETVYEWKFLRLVYDKLVRLDENFEPQPWAAEEINVVEGEDGEVIDVTLRDDLQFHDGE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCC
PLGPEDVVFTFDYMFEEGIPYFQAFLDPIETVDLMDDDETIRFTLEEAYAPFITNTLGQI
CCCCCCEEEEEHHHHHCCCHHHHHHHCHHHHHHCCCCCCEEEEEHHHHHCHHHHHHHCCC
PILPEHIWADVMEEENLDHPSQFDNAEAIGSGPFKFDNWERGEYIRIVKNDDYFKADDID
CCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEECCCCEECCCCC
VEAIRYDKYSHSEGVFGALENQQADVNENTFDPEYVQQAEDLDHLTVVREPDIGFDFIGL
EEEEEECCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEEEC
NNYKEPFNDKAVRQAAAHAIDLDELVDVLLYGYGDPAGAGQTISTGNEMWKNDDVKEYPF
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
DIDKAREILKDAGYEWDSDGRLYFPEE
CHHHHHHHHHHCCCCCCCCCEEECCCC
>Mature Secondary Structure
MENAGQFFKKFSVLMAVLIVTTALVIGCGDGGDPDASDDLDPDRKVPEVRFVSSTADDNQ
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
IRNEAVQLVADWWEEIGLEVDIQTREFNSLVNRVLAAPEDKDFEAYILGWSGRVSRSDPD
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCC
MFLYSLYHSNQAVDGGNNSSVFKNEKYDELVSKQRAEMDLEKRQELVFEAQEVLAEEVPD
CEEEEEHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
ITLYYRDEIQGYNNERWEDLPSMAGEGIFNEQFPYEATPKTDDAEFVIANSANLDTFNPF
EEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCH
AAETVYEWKFLRLVYDKLVRLDENFEPQPWAAEEINVVEGEDGEVIDVTLRDDLQFHDGE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCC
PLGPEDVVFTFDYMFEEGIPYFQAFLDPIETVDLMDDDETIRFTLEEAYAPFITNTLGQI
CCCCCCEEEEEHHHHHCCCHHHHHHHCHHHHHHCCCCCCEEEEEHHHHHCHHHHHHHCCC
PILPEHIWADVMEEENLDHPSQFDNAEAIGSGPFKFDNWERGEYIRIVKNDDYFKADDID
CCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEECCCCEECCCCC
VEAIRYDKYSHSEGVFGALENQQADVNENTFDPEYVQQAEDLDHLTVVREPDIGFDFIGL
EEEEEECCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEEEC
NNYKEPFNDKAVRQAAAHAIDLDELVDVLLYGYGDPAGAGQTISTGNEMWKNDDVKEYPF
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
DIDKAREILKDAGYEWDSDGRLYFPEE
CHHHHHHHHHHCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]