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The map label for this gene is lytC [H]

Identifier: 188586792

GI number: 188586792

Start: 2312199

End: 2313494

Strand: Direct

Name: lytC [H]

Synonym: Nther_2183

Alternate gene names: 188586792

Gene position: 2312199-2313494 (Clockwise)

Preceding gene: 188586790

Following gene: 188586799

Centisome position: 73.04

GC content: 38.97

Gene sequence:

>1296_bases
ATGAATAAAGTTAAGCTAGGCCTTTCTATGTTAGTCTTTCTCACTGTTTTAACTATGGTAAGCGGTGTGTTAGCAGGACA
GGAAGTTAAGGTGTTTGTCGATAACGAGGAAATAAACTTTCCAGATCAAAACCCGTACATAGACCAAAACAACAGAACTC
AAGTTCCAGTCAGATTTGTCAGTCAAGCTCTAGGAGGATATGTTGAGTGGCATAGTGATAGCCAAGAAATTGAGATAAAA
TTGGGGGACGATCGATTAATCTTAGAAGTAGGTATCCAAAATTACAGTTTAAATGGACAGGCACTGGATATGGATACAGC
TCCATTAATAACCAATCAAGGCCGGACTATGGTTCCACTGAGGTTTGTTAGTGAGGGACTTGGGGCACAAGTTAATTGGG
TAGAAGAAGAGAATTCTGTTTATATTTCACAGAGGGAAAATAATCACCAAATTGAAGATCTAGAACAGGCTGAGGTAACC
GCTACTACATTAAATGTAAGAACTGGCCCAGGACTAAACCACGCACCAATCCATCAAGTTCATGCTAATGAAACTTATCC
AGTATTAGAAGAAGTTTCGTATGAGTACGATAATACTCATCATGAATGGATGAAAATCAATTTACCAGAACACAATGAAG
TAGGATGGGTAAGTAGAGACTTTGTAAGATTGGCTGATGATAATGATGGAGTAACCGAGGACACGAACAAGCAAGAGGGA
CATGAAGGATCACCCTTACAAGGTTTTAAAATAGTTATTGACCCGGGACATGGCGGAACTGACCCTGGAGCAGTTGGGCC
TACTGGGCTAACAGAAAAGGAAGTTGCTTTAGATGTATCCCTGAGGGCAAGAGATCTACTTGAGGACTTAGGTGCTGAAA
CATACCTTACTAGGTATTCTGATATTGATGTAACACTATATGACAGAATCAATTTGGCTAATCAAATTAACGCCGATATT
TTCATAAGTGTTCATGCTAATGCAGCATTAAATAGATCTGCCCAAGGAACTGAAACTTATTACTCTTCACAAAGAAGTCT
TTACGACTATAACTTGGCAAATAGCCTACAAACAGCTTTAGTAAACAAACTTGGTACAATAGATAGAGGTACTCTGGATA
GAAGTTTCTATGTGCTCAGGCACGGAAATATGCCTTCGGCTTTAGTAGAGCTGGCTTTCGTTTCTAATTACTGGGAGGAA
TCTCTATTAAAAGACAATTATTTTAGACAAAACGCAGCCCAGGCCATTACCGATGGTGTTTATAATTATTTTAAAAGCAT
CTCTCAAAGTTACTAG

Upstream 100 bases:

>100_bases
CCCCAGTCCCCTGGCGAAAGTTCATTTAATTTCTAACGGACCTTCCAGAGTAAGACCTAATAATTTCAGAAATCAAAATG
AGAAAGGAGATTTGTTAAAA

Downstream 100 bases:

>100_bases
CCTACCTCACTTAACACTTAACAGGAAATGGCATAAAATCAACCCTTCATATAGAAGCTGATTAGTTAATAAATTGATGA
AATAACAAAACATATTATAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNKVKLGLSMLVFLTVLTMVSGVLAGQEVKVFVDNEEINFPDQNPYIDQNNRTQVPVRFVSQALGGYVEWHSDSQEIEIK
LGDDRLILEVGIQNYSLNGQALDMDTAPLITNQGRTMVPLRFVSEGLGAQVNWVEEENSVYISQRENNHQIEDLEQAEVT
ATTLNVRTGPGLNHAPIHQVHANETYPVLEEVSYEYDNTHHEWMKINLPEHNEVGWVSRDFVRLADDNDGVTEDTNKQEG
HEGSPLQGFKIVIDPGHGGTDPGAVGPTGLTEKEVALDVSLRARDLLEDLGAETYLTRYSDIDVTLYDRINLANQINADI
FISVHANAALNRSAQGTETYYSSQRSLYDYNLANSLQTALVNKLGTIDRGTLDRSFYVLRHGNMPSALVELAFVSNYWEE
SLLKDNYFRQNAAQAITDGVYNYFKSISQSY

Sequences:

>Translated_431_residues
MNKVKLGLSMLVFLTVLTMVSGVLAGQEVKVFVDNEEINFPDQNPYIDQNNRTQVPVRFVSQALGGYVEWHSDSQEIEIK
LGDDRLILEVGIQNYSLNGQALDMDTAPLITNQGRTMVPLRFVSEGLGAQVNWVEEENSVYISQRENNHQIEDLEQAEVT
ATTLNVRTGPGLNHAPIHQVHANETYPVLEEVSYEYDNTHHEWMKINLPEHNEVGWVSRDFVRLADDNDGVTEDTNKQEG
HEGSPLQGFKIVIDPGHGGTDPGAVGPTGLTEKEVALDVSLRARDLLEDLGAETYLTRYSDIDVTLYDRINLANQINADI
FISVHANAALNRSAQGTETYYSSQRSLYDYNLANSLQTALVNKLGTIDRGTLDRSFYVLRHGNMPSALVELAFVSNYWEE
SLLKDNYFRQNAAQAITDGVYNYFKSISQSY
>Mature_431_residues
MNKVKLGLSMLVFLTVLTMVSGVLAGQEVKVFVDNEEINFPDQNPYIDQNNRTQVPVRFVSQALGGYVEWHSDSQEIEIK
LGDDRLILEVGIQNYSLNGQALDMDTAPLITNQGRTMVPLRFVSEGLGAQVNWVEEENSVYISQRENNHQIEDLEQAEVT
ATTLNVRTGPGLNHAPIHQVHANETYPVLEEVSYEYDNTHHEWMKINLPEHNEVGWVSRDFVRLADDNDGVTEDTNKQEG
HEGSPLQGFKIVIDPGHGGTDPGAVGPTGLTEKEVALDVSLRARDLLEDLGAETYLTRYSDIDVTLYDRINLANQINADI
FISVHANAALNRSAQGTETYYSSQRSLYDYNLANSLQTALVNKLGTIDRGTLDRSFYVLRHGNMPSALVELAFVSNYWEE
SLLKDNYFRQNAAQAITDGVYNYFKSISQSY

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=220, Percent_Identity=32.2727272727273, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082163, Length=218, Percent_Identity=33.0275229357798, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1790611, Length=223, Percent_Identity=31.8385650224215, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 48325; Mature: 48325

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVKLGLSMLVFLTVLTMVSGVLAGQEVKVFVDNEEINFPDQNPYIDQNNRTQVPVRFV
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
SQALGGYVEWHSDSQEIEIKLGDDRLILEVGIQNYSLNGQALDMDTAPLITNQGRTMVPL
HHHHCCEEEECCCCCEEEEEECCCEEEEEECCCEECCCCEEECCCCCCEEECCCCEEEEH
RFVSEGLGAQVNWVEEENSVYISQRENNHQIEDLEQAEVTATTLNVRTGPGLNHAPIHQV
HHHHHCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHEEEEEEEEEECCCCCCCCCCEEE
HANETYPVLEEVSYEYDNTHHEWMKINLPEHNEVGWVSRDFVRLADDNDGVTEDTNKQEG
ECCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCEECCEEEEECCCCCCCCCCCCCCC
HEGSPLQGFKIVIDPGHGGTDPGAVGPTGLTEKEVALDVSLRARDLLEDLGAETYLTRYS
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEECC
DIDVTLYDRINLANQINADIFISVHANAALNRSAQGTETYYSSQRSLYDYNLANSLQTAL
CCCEEEEECCCHHHHCCCEEEEEEECCCEECCCCCCCHHHHHCCCCEEECHHHHHHHHHH
VNKLGTIDRGTLDRSFYVLRHGNMPSALVELAFVSNYWEESLLKDNYFRQNAAQAITDGV
HHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNYFKSISQSY
HHHHHHHHCCC
>Mature Secondary Structure
MNKVKLGLSMLVFLTVLTMVSGVLAGQEVKVFVDNEEINFPDQNPYIDQNNRTQVPVRFV
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHH
SQALGGYVEWHSDSQEIEIKLGDDRLILEVGIQNYSLNGQALDMDTAPLITNQGRTMVPL
HHHHCCEEEECCCCCEEEEEECCCEEEEEECCCEECCCCEEECCCCCCEEECCCCEEEEH
RFVSEGLGAQVNWVEEENSVYISQRENNHQIEDLEQAEVTATTLNVRTGPGLNHAPIHQV
HHHHHCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHEEEEEEEEEECCCCCCCCCCEEE
HANETYPVLEEVSYEYDNTHHEWMKINLPEHNEVGWVSRDFVRLADDNDGVTEDTNKQEG
ECCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCCCCEECCEEEEECCCCCCCCCCCCCCC
HEGSPLQGFKIVIDPGHGGTDPGAVGPTGLTEKEVALDVSLRARDLLEDLGAETYLTRYS
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEECC
DIDVTLYDRINLANQINADIFISVHANAALNRSAQGTETYYSSQRSLYDYNLANSLQTAL
CCCEEEEECCCHHHHCCCEEEEEEECCCEECCCCCCCHHHHHCCCCEEECHHHHHHHHHH
VNKLGTIDRGTLDRSFYVLRHGNMPSALVELAFVSNYWEESLLKDNYFRQNAAQAITDGV
HHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNYFKSISQSY
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]