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The map label for this gene is lpdA [H]

Identifier: 188586789

GI number: 188586789

Start: 2305638

End: 2307395

Strand: Direct

Name: lpdA [H]

Synonym: Nther_2180

Alternate gene names: 188586789

Gene position: 2305638-2307395 (Clockwise)

Preceding gene: 188586787

Following gene: 188586790

Centisome position: 72.84

GC content: 36.8

Gene sequence:

>1758_bases
ATGGATTTTTTAGTTAAAATTCCAAAATTGAATCAAAAAAGTAACCAAGTCACGGTATATCAATGGTTCATACAAGAAGG
GGAAGAGGTCAAATCTGGAGATCCGCTTCTAGAAGTGACATCGGATAAAACCAATGTGCGAGTTGAAGCTGAAGTTAATG
GAACAGTAAAAGAGATAATGGTTTCTGAAGGGGAAAAAGCAAAACTCAATCAAGCCGTTTGTAAATTAGAAACAACTGAA
ACAGTGTCAAAAGATAAGCCTAAAAGTTCACCCAAAGATAAAGATACATCCAAGACTAGTGGTTCAGATGAATCTCAATT
TAACTACTTTGGTAGCTTACTTGGCAAAAAAGAACCCGAAAAAGAAAGTAAAGAAGTTGATTTATGTATCTTAGGAGGGG
GACCCGGAGGATATACAGCAGCTATCAGAGCTGCCAAAGCAGGTTTATCTGTAGCTCTCGTGGAAAAAGACAATTTAGGA
GGAACCTGCTTAAATAGAGGTTGTATACCAACAAAAGCCCTTATCCAATCCGCTAATCTCTTAAGTCAAATAAATTCTGC
CGAAAACTTTGGAATTACTACTGATGCGGTAATAGGAGATTTTTCAAAAGCAGTTTCTTATAAAGATAATGTAGTTACTA
CTCTAAAAGATGGAGTTGCAAATCTGTTAGCCAACAACCAGGTTTCAGTCATTTCTGGTGAAGGACAACTCAAATCATCT
ACTGAAGTGACAGTTGAAGCTGACAATAAGATAGTAAATATAACAGCTAAAAATATCATTTTAGCAACAGGGTCAAAACC
TAAGCTCCCTCCAATTGAAGGAATAGAACACGAAAATGTTTTAACCAGTACAGAATTACTCAATTTAAAGGAATTGCCAT
CAAAAATGGTAATTATCGGTGGCGGCGTTATCGGTATGGAACTATCATTTATTTTAAATAAATTCGGTGTTGATGTGACT
GTAGTAGAAGCAATGGACCATATCCTGCCTTATATGGATAAACAAATTGGTGAAGAAATTAAAATCAGTGCCGAAGAACA
AGGCATCAATATTATTACAGGAGCTTTTGCAAAAGAAATTTCTCAAGTTGAAAATGGCGGTCTAATGATCACCTTAACTA
AAAATGAAGAACAATTAAAAGTTTTTGGTAACCAAATCCTTGTGGCCATAGGAAGAGATTTCAATACTAAAGCTTTAAAT
GCAACCGAGCTTGGACTGGACACAACAAAACAAGGTGCTATTCAGGTGGACAAACACCTAAAAACAAGTCTAGACAATGT
ATATGCCATAGGTGATGTGATTGGAGGTTATCTCCTGGCCCATGAAGCTTCTCATGAAGCCATTGTTGCCGTTGATAATA
TTCTCGGAATAGAAAAACAGATAAACTACGATAATATACCATCGGCTGTGTTCACTGATCCTGAAGCTGCCCAAGTAGGA
TTGACTCAAAAAGAGGCCAAAGATATGGGATATCAGGTTAAAACAGCTGAATTCCCCTTTTCTTCCAATGGTAAAGTTTT
AACGACGCAGAAGCTGTCCGGATTTAGCAAATTAGTTGTGAATTCTACAGATAACGTAATTTTAGGGGGTAGCCTGGTTG
GAGTAGGTGCTACTGAACTCATCCATCAAATTGCAATAGCAGTAACTAATCAATTAACAGTAGAACAGTTAACAAACACA
GTTTTTGCTCACCCCACCGTGTCAGAAACAATTTTTGAAAGCTCTTTAGCTTTTTTTGGAGAAGAAATCCATGGTTAA

Upstream 100 bases:

>100_bases
GTTTTGATTTCTGTCTAAAACTATCTTAACTCCGGGACGGAGACTGATTCAAATATCATATAACTTAACAGAACTATTAT
ATTATACAGGAGGTAGATTT

Downstream 100 bases:

>100_bases
TTTTCTAAACCAAAACAGGTATTGATTAAAATTTATCGAATAATAATTATGAATCTATTTACAGTTACGCTGAAAACGAG
CATCAACATTAATGAGCATT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MDFLVKIPKLNQKSNQVTVYQWFIQEGEEVKSGDPLLEVTSDKTNVRVEAEVNGTVKEIMVSEGEKAKLNQAVCKLETTE
TVSKDKPKSSPKDKDTSKTSGSDESQFNYFGSLLGKKEPEKESKEVDLCILGGGPGGYTAAIRAAKAGLSVALVEKDNLG
GTCLNRGCIPTKALIQSANLLSQINSAENFGITTDAVIGDFSKAVSYKDNVVTTLKDGVANLLANNQVSVISGEGQLKSS
TEVTVEADNKIVNITAKNIILATGSKPKLPPIEGIEHENVLTSTELLNLKELPSKMVIIGGGVIGMELSFILNKFGVDVT
VVEAMDHILPYMDKQIGEEIKISAEEQGINIITGAFAKEISQVENGGLMITLTKNEEQLKVFGNQILVAIGRDFNTKALN
ATELGLDTTKQGAIQVDKHLKTSLDNVYAIGDVIGGYLLAHEASHEAIVAVDNILGIEKQINYDNIPSAVFTDPEAAQVG
LTQKEAKDMGYQVKTAEFPFSSNGKVLTTQKLSGFSKLVVNSTDNVILGGSLVGVGATELIHQIAIAVTNQLTVEQLTNT
VFAHPTVSETIFESSLAFFGEEIHG

Sequences:

>Translated_585_residues
MDFLVKIPKLNQKSNQVTVYQWFIQEGEEVKSGDPLLEVTSDKTNVRVEAEVNGTVKEIMVSEGEKAKLNQAVCKLETTE
TVSKDKPKSSPKDKDTSKTSGSDESQFNYFGSLLGKKEPEKESKEVDLCILGGGPGGYTAAIRAAKAGLSVALVEKDNLG
GTCLNRGCIPTKALIQSANLLSQINSAENFGITTDAVIGDFSKAVSYKDNVVTTLKDGVANLLANNQVSVISGEGQLKSS
TEVTVEADNKIVNITAKNIILATGSKPKLPPIEGIEHENVLTSTELLNLKELPSKMVIIGGGVIGMELSFILNKFGVDVT
VVEAMDHILPYMDKQIGEEIKISAEEQGINIITGAFAKEISQVENGGLMITLTKNEEQLKVFGNQILVAIGRDFNTKALN
ATELGLDTTKQGAIQVDKHLKTSLDNVYAIGDVIGGYLLAHEASHEAIVAVDNILGIEKQINYDNIPSAVFTDPEAAQVG
LTQKEAKDMGYQVKTAEFPFSSNGKVLTTQKLSGFSKLVVNSTDNVILGGSLVGVGATELIHQIAIAVTNQLTVEQLTNT
VFAHPTVSETIFESSLAFFGEEIHG
>Mature_585_residues
MDFLVKIPKLNQKSNQVTVYQWFIQEGEEVKSGDPLLEVTSDKTNVRVEAEVNGTVKEIMVSEGEKAKLNQAVCKLETTE
TVSKDKPKSSPKDKDTSKTSGSDESQFNYFGSLLGKKEPEKESKEVDLCILGGGPGGYTAAIRAAKAGLSVALVEKDNLG
GTCLNRGCIPTKALIQSANLLSQINSAENFGITTDAVIGDFSKAVSYKDNVVTTLKDGVANLLANNQVSVISGEGQLKSS
TEVTVEADNKIVNITAKNIILATGSKPKLPPIEGIEHENVLTSTELLNLKELPSKMVIIGGGVIGMELSFILNKFGVDVT
VVEAMDHILPYMDKQIGEEIKISAEEQGINIITGAFAKEISQVENGGLMITLTKNEEQLKVFGNQILVAIGRDFNTKALN
ATELGLDTTKQGAIQVDKHLKTSLDNVYAIGDVIGGYLLAHEASHEAIVAVDNILGIEKQINYDNIPSAVFTDPEAAQVG
LTQKEAKDMGYQVKTAEFPFSSNGKVLTTQKLSGFSKLVVNSTDNVILGGSLVGVGATELIHQIAIAVTNQLTVEQLTNT
VFAHPTVSETIFESSLAFFGEEIHG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=34.468085106383, Blast_Score=276, Evalue=6e-74,
Organism=Homo sapiens, GI50301238, Length=444, Percent_Identity=30.1801801801802, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI22035672, Length=462, Percent_Identity=29.4372294372294, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI33519430, Length=441, Percent_Identity=25.8503401360544, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI33519428, Length=441, Percent_Identity=25.8503401360544, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI33519426, Length=441, Percent_Identity=25.8503401360544, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI148277071, Length=441, Percent_Identity=25.8503401360544, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI148277065, Length=441, Percent_Identity=25.8503401360544, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=26.1574074074074, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI291045268, Length=427, Percent_Identity=24.824355971897, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786307, Length=456, Percent_Identity=33.7719298245614, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI87081717, Length=448, Percent_Identity=32.8125, Blast_Score=215, Evalue=8e-57,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=27.1948608137045, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=27.7136258660508, Blast_Score=171, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI32565766, Length=465, Percent_Identity=32.9032258064516, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI71983429, Length=447, Percent_Identity=29.7539149888143, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71983419, Length=447, Percent_Identity=29.7539149888143, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17557007, Length=488, Percent_Identity=28.0737704918033, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71982272, Length=345, Percent_Identity=28.9855072463768, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=485, Percent_Identity=34.639175257732, Blast_Score=260, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=29.723991507431, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=26.7241379310345, Blast_Score=147, Evalue=5e-36,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=34.6723044397463, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=28.8421052631579, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24640553, Length=471, Percent_Identity=28.8747346072187, Blast_Score=159, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24640551, Length=471, Percent_Identity=28.8747346072187, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI17737741, Length=495, Percent_Identity=26.6666666666667, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 62604; Mature: 62604

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLVKIPKLNQKSNQVTVYQWFIQEGEEVKSGDPLLEVTSDKTNVRVEAEVNGTVKEIM
CCCEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCHHHHH
VSEGEKAKLNQAVCKLETTETVSKDKPKSSPKDKDTSKTSGSDESQFNYFGSLLGKKEPE
HCCCCHHHHHHHHHEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
KESKEVDLCILGGGPGGYTAAIRAAKAGLSVALVEKDNLGGTCLNRGCIPTKALIQSANL
CCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
LSQINSAENFGITTDAVIGDFSKAVSYKDNVVTTLKDGVANLLANNQVSVISGEGQLKSS
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
TEVTVEADNKIVNITAKNIILATGSKPKLPPIEGIEHENVLTSTELLNLKELPSKMVIIG
CEEEEEECCEEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEE
GGVIGMELSFILNKFGVDVTVVEAMDHILPYMDKQIGEEIKISAEEQGINIITGAFAKEI
CCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHH
SQVENGGLMITLTKNEEQLKVFGNQILVAIGRDFNTKALNATELGLDTTKQGAIQVDKHL
HHHCCCCEEEEEECCCHHHHHHCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCEEEHHHH
KTSLDNVYAIGDVIGGYLLAHEASHEAIVAVDNILGIEKQINYDNIPSAVFTDPEAAQVG
HHHHHHHEEHHHHHHHHHEEECCCCCEEEEEHHHHCCHHCCCCCCCCCCEECCCCHHHCC
LTQKEAKDMGYQVKTAEFPFSSNGKVLTTQKLSGFSKLVVNSTDNVILGGSLVGVGATEL
CCHHHHHHCCCEEEEEECCCCCCCCEEEEHHHCCCEEEEECCCCCEEECCCEECCCHHHH
IHQIAIAVTNQLTVEQLTNTVFAHPTVSETIFESSLAFFGEEIHG
HHHHHHHHHCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFLVKIPKLNQKSNQVTVYQWFIQEGEEVKSGDPLLEVTSDKTNVRVEAEVNGTVKEIM
CCCEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCHHHHH
VSEGEKAKLNQAVCKLETTETVSKDKPKSSPKDKDTSKTSGSDESQFNYFGSLLGKKEPE
HCCCCHHHHHHHHHEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
KESKEVDLCILGGGPGGYTAAIRAAKAGLSVALVEKDNLGGTCLNRGCIPTKALIQSANL
CCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
LSQINSAENFGITTDAVIGDFSKAVSYKDNVVTTLKDGVANLLANNQVSVISGEGQLKSS
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
TEVTVEADNKIVNITAKNIILATGSKPKLPPIEGIEHENVLTSTELLNLKELPSKMVIIG
CEEEEEECCEEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEE
GGVIGMELSFILNKFGVDVTVVEAMDHILPYMDKQIGEEIKISAEEQGINIITGAFAKEI
CCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHH
SQVENGGLMITLTKNEEQLKVFGNQILVAIGRDFNTKALNATELGLDTTKQGAIQVDKHL
HHHCCCCEEEEEECCCHHHHHHCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCEEEHHHH
KTSLDNVYAIGDVIGGYLLAHEASHEAIVAVDNILGIEKQINYDNIPSAVFTDPEAAQVG
HHHHHHHEEHHHHHHHHHEEECCCCCEEEEEHHHHCCHHCCCCCCCCCCEECCCCHHHCC
LTQKEAKDMGYQVKTAEFPFSSNGKVLTTQKLSGFSKLVVNSTDNVILGGSLVGVGATEL
CCHHHHHHCCCEEEEEECCCCCCCCEEEEHHHCCCEEEEECCCCCEEECCCEECCCHHHH
IHQIAIAVTNQLTVEQLTNTVFAHPTVSETIFESSLAFFGEEIHG
HHHHHHHHHCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]