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The map label for this gene is ndhF [H]

Identifier: 188586762

GI number: 188586762

Start: 2278007

End: 2278885

Strand: Reverse

Name: ndhF [H]

Synonym: Nther_2153

Alternate gene names: 188586762

Gene position: 2278885-2278007 (Counterclockwise)

Preceding gene: 188586763

Following gene: 188586761

Centisome position: 71.99

GC content: 36.52

Gene sequence:

>879_bases
ATGATATTATCCGAGTTTGATTATTTAGCTCCCAAGTCACTGTCAGAAGCTCTTAATATACTAGATAAAGCTGACAATGA
GCCATGTCTTTTGGCAGGTGGCACAGATGTGATGATTCACTTAAAGGAAGAAGAAATAAAGCCTCAACAAGTGATTAATT
TAAAGCAAATTCCCGAGCTTAATTTTATTAAAGAAGAAAACGGTTATATTAAGATCGGTGCCATGACTACTTTTAGTACA
ATTGAAAAATCAAATCTAATTCAAGATAGAGCCAAAGCACTAGCAAAGGCTTGTGGAGAAGTGGGGTCACCGCAAATCAG
AAATACAGGCACTGTTGGCGGAAATATAGTTAATGCTTCTCCCGCTGCTGATTCTGTGCCAGCACTTATGGCTTTAGATG
CCAAGGTGGTTTTGGCTAACAAAGAAGGTGAAAGAGAATTAGCTTTAGATGAGTTTTTATGCGGTATTAATCAAACTGTG
ATAAAGCCCAATGAAATACTCACTCACATTTATTTTAAGACACCTAAGATTGATAGCATTAGTGAATTTGAAAAGCTAGG
TCGTCGTAAGGCCTTGGCTATAGCCAGATTAAGTGTAGGTTTTTACGCTGAAGTTGAACCTGAGAACAAAGTTTTACAGG
ATGTAAGGATTTCACTGGGAGCAGTAGCTAAAAATCCTTTTAGAGCAGTGGAAGCGGAAGAATATTTAAAAGGTAAAAAG
TTAAATGAAGATGTTATCACTGAAACAGTAGAGAAAATTTCTGAAATAGCTGCAGTAACTTTAGGTAACAGATCTTCAGC
TCCTTTTAAACGAGAAAGTATCAAAGGAGTTGCTAAAAAAGCTTTAATTAAGGCCATGGATTCGGCAGACAAAATTTAA

Upstream 100 bases:

>100_bases
ACAATTTAACGAATTAATTGGACTTAAAGAGATCAAAGACTTAGAAGGAAAATTTGTCAGAGGCCAGTAAAATCACCTGA
ATATGAGGAGGGAAAAGCCA

Downstream 100 bases:

>100_bases
GGAGGGGCAGAGAATGAATAAAACAGTGAAGATCTCCTTTGAATTAAATGGGAAACAGGTAGATGTAGAGACTAAACCCA
ATAAACGCTTATTGGACCTG

Product: molybdopterin dehydrogenase FAD-binding

Products: NA

Alternate protein names: NDH; Nicotinic acid hydroxylase FAD-subunit; NAH [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MILSEFDYLAPKSLSEALNILDKADNEPCLLAGGTDVMIHLKEEEIKPQQVINLKQIPELNFIKEENGYIKIGAMTTFST
IEKSNLIQDRAKALAKACGEVGSPQIRNTGTVGGNIVNASPAADSVPALMALDAKVVLANKEGERELALDEFLCGINQTV
IKPNEILTHIYFKTPKIDSISEFEKLGRRKALAIARLSVGFYAEVEPENKVLQDVRISLGAVAKNPFRAVEAEEYLKGKK
LNEDVITETVEKISEIAAVTLGNRSSAPFKRESIKGVAKKALIKAMDSADKI

Sequences:

>Translated_292_residues
MILSEFDYLAPKSLSEALNILDKADNEPCLLAGGTDVMIHLKEEEIKPQQVINLKQIPELNFIKEENGYIKIGAMTTFST
IEKSNLIQDRAKALAKACGEVGSPQIRNTGTVGGNIVNASPAADSVPALMALDAKVVLANKEGERELALDEFLCGINQTV
IKPNEILTHIYFKTPKIDSISEFEKLGRRKALAIARLSVGFYAEVEPENKVLQDVRISLGAVAKNPFRAVEAEEYLKGKK
LNEDVITETVEKISEIAAVTLGNRSSAPFKRESIKGVAKKALIKAMDSADKI
>Mature_292_residues
MILSEFDYLAPKSLSEALNILDKADNEPCLLAGGTDVMIHLKEEEIKPQQVINLKQIPELNFIKEENGYIKIGAMTTFST
IEKSNLIQDRAKALAKACGEVGSPQIRNTGTVGGNIVNASPAADSVPALMALDAKVVLANKEGERELALDEFLCGINQTV
IKPNEILTHIYFKTPKIDSISEFEKLGRRKALAIARLSVGFYAEVEPENKVLQDVRISLGAVAKNPFRAVEAEEYLKGKK
LNEDVITETVEKISEIAAVTLGNRSSAPFKRESIKGVAKKALIKAMDSADKI

Specific function: Catalyzes the hydroxylation of nicotinate to 6- hydroxynicotinate. Also active against 2-pyrazinecarboxylic acid, but inactive against other nicotinate analogs [H]

COG id: COG1319

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, middle subunit CoxM/CutM homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI91823271, Length=238, Percent_Identity=25.6302521008403, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI71773480, Length=250, Percent_Identity=24.8, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789231, Length=285, Percent_Identity=30.1754385964912, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789245, Length=240, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540638, Length=239, Percent_Identity=28.0334728033473, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17737937, Length=277, Percent_Identity=25.6317689530686, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24647199, Length=267, Percent_Identity=29.2134831460674, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005107
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR002346 [H]

Pfam domain/function: PF03450 CO_deh_flav_C; PF00941 FAD_binding_5 [H]

EC number: =1.17.1.5 [H]

Molecular weight: Translated: 31851; Mature: 31851

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSEFDYLAPKSLSEALNILDKADNEPCLLAGGTDVMIHLKEEEIKPQQVINLKQIPEL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEHHHCCHHHHCCHHHCCCC
NFIKEENGYIKIGAMTTFSTIEKSNLIQDRAKALAKACGEVGSPQIRNTGTVGGNIVNAS
CEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECC
PAADSVPALMALDAKVVLANKEGERELALDEFLCGINQTVIKPNEILTHIYFKTPKIDSI
CCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCEECHHHHHEEEEEECCCCCCH
SEFEKLGRRKALAIARLSVGFYAEVEPENKVLQDVRISLGAVAKNPFRAVEAEEYLKGKK
HHHHHHHHHHHHHHHHEEECEEEEECCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHCCCC
LNEDVITETVEKISEIAAVTLGNRSSAPFKRESIKGVAKKALIKAMDSADKI
CCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILSEFDYLAPKSLSEALNILDKADNEPCLLAGGTDVMIHLKEEEIKPQQVINLKQIPEL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEHHHCCHHHHCCHHHCCCC
NFIKEENGYIKIGAMTTFSTIEKSNLIQDRAKALAKACGEVGSPQIRNTGTVGGNIVNAS
CEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECC
PAADSVPALMALDAKVVLANKEGERELALDEFLCGINQTVIKPNEILTHIYFKTPKIDSI
CCCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCEECHHHHHEEEEEECCCCCCH
SEFEKLGRRKALAIARLSVGFYAEVEPENKVLQDVRISLGAVAKNPFRAVEAEEYLKGKK
HHHHHHHHHHHHHHHHEEECEEEEECCHHHHHHHHHHHHHHHCCCCHHHCCHHHHHCCCC
LNEDVITETVEKISEIAAVTLGNRSSAPFKRESIKGVAKKALIKAMDSADKI
CCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA