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The map label for this gene is epsE [H]

Identifier: 188586713

GI number: 188586713

Start: 2219277

End: 2220992

Strand: Reverse

Name: epsE [H]

Synonym: Nther_2103

Alternate gene names: 188586713

Gene position: 2220992-2219277 (Counterclockwise)

Preceding gene: 188586714

Following gene: 188586712

Centisome position: 70.16

GC content: 39.92

Gene sequence:

>1716_bases
GTGTTTCGTCAGAAGAAACAAAGATTAGGGGATTTGTTGTTGGAAAGCGGTGCTATCACCGAAGAAGACTTAAAACAAGC
CCTTGACCATCAAAATAAGTCAGGACAAAAGTTAGGGGCTAGCCTGGTAGATCTGGGAATAATTACAGAAGAAGAGATTA
TTGAAGTATTGGAATTCCAACTTGGAATACCCCATGTATCCCTGTCCCAATACGATACCAATAGAGAAACTGCTACACTC
ATTCCTGCCTATTTAGCAGAACGGTATCAAGTGCTTCCCATAGATAATAGAAGTGGAAAATTAGTACTGGCCATGGGTGA
CCCTTTAAATGTGGTTGCTATTGATGACGTCAAAATGGCTACCGGTATGGAAGTTGAGCCTGTTATAGCATCTCCTCGAG
AAATCGAAGGTGAGATTAACCGTCACTTTGGAATCCAGGATTCTGTTGAAAAAGCTATAGAAGAAATTGAAGGCTCAGCC
GAGGAAGAGGCAGAAAGTGAAATAGCTGCAACTGAAGAAGAAGAGTTATCAAATCTAGAAACTAATGCACCTGTTGTCAA
AGTGGTCAATTCTTTAGTGTCTCAAGCATACGAGCAGGGTGCTAGTGATATACATATTGAACCTACTAAACAGGGGATGC
AGATACGGTACCGAATAGATGGAGTCTTGCATAATGTGGCAACTCCTCCCCGGTATGCTAAAGATCTGTTGATCAGTCGT
GTGAAAATTATGGCTGGTATGGACATTACAAAAAAAAGAATCCCCCAAGATGGTAGGAGCAATTATAATATAGGAGGACA
TGAAATTGACTTAAGGGTATCTACTTTACCGACAATTTACGGTGAAAAAGTAGTGATCCGCTTGCTTCATAAAGATAAAG
TGATTTTTTCACTGGATAAATTGGGGTTTCAACAGGATAATTTTAAGCTCTATCAAGGATTATTGAAAAACAGTGCCGGG
ATGGTTTTGGTTACCGGCCCTACTGGATGTGGTAAAACTACTACTCTCTATTCTTCCCTCAACCGGATAAACAGTTCCGA
GAAAAATATAATTACTATAGAGGATCCAGTGGAATATCAGATTGAAGGGATTAATCAAGTTCAAACCAATGAAAAAGGTG
GATTGACCTTCGCAAACGGTTTAAGAGCTATTTTACGTCAAGATCCGGATATCATCATGGTAGGGGAAATCAGAGACTTA
GAAACTGCTCAAATTGCAATTAGATCGGCCCTGACAGGACATTTAGTGTTTTCTACATTACATACTAATAATGCGATTGC
CACCCTTTCCAGGCTAGTGGATATGGGAATCCCGCCTTTTTTAGTTAGCTCTGCTGTGGAAGGAGTATTATCCCAGAGGT
TGGTAAGGATAATCTGTTCCAATTGCAAAATTGAATACAGCCCCACGGCCGAGGAACAAGAAATATATCACCGTTACTCG
GGGGAACAGGTGGATACCCTTTATAAAGGTAAAGGCTGTACCAACTGTAATAATACTGGTTATAAAGGTCGGACTGCAAT
TCATGAACTATTGATACTTGATAAAACTTTAAAAGATATGCTAGCTAGAGAAGCTTCTGAAAGAGAACTGACAGAGGAAG
CTCGTAAAAGAGGTTTTTCATATTTAATCGAAAACGCCATTTCTAAAGTCAGTCAGGGTATCACAACTATGGAAGAAGTT
ATTAGAGCTACCTTTCATCAGGAAAGCCATCTCTAA

Upstream 100 bases:

>100_bases
CTAGTATACTATACAGTATTTACAGGGCTTTGAGTTATCAATTATAATGAAAAGATAGTAAGTATAACTAATTTTATTTT
TGAAAGAGGTGGCTAGTTAA

Downstream 100 bases:

>100_bases
ATAACTCATCGTCGACTTATGAATAATATTGATTCATCGACTAATAATTGAATTTATCGATAATTATTGAATCACTGCGC
AGATTTGATTTATGTCTTCC

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MFRQKKQRLGDLLLESGAITEEDLKQALDHQNKSGQKLGASLVDLGIITEEEIIEVLEFQLGIPHVSLSQYDTNRETATL
IPAYLAERYQVLPIDNRSGKLVLAMGDPLNVVAIDDVKMATGMEVEPVIASPREIEGEINRHFGIQDSVEKAIEEIEGSA
EEEAESEIAATEEEELSNLETNAPVVKVVNSLVSQAYEQGASDIHIEPTKQGMQIRYRIDGVLHNVATPPRYAKDLLISR
VKIMAGMDITKKRIPQDGRSNYNIGGHEIDLRVSTLPTIYGEKVVIRLLHKDKVIFSLDKLGFQQDNFKLYQGLLKNSAG
MVLVTGPTGCGKTTTLYSSLNRINSSEKNIITIEDPVEYQIEGINQVQTNEKGGLTFANGLRAILRQDPDIIMVGEIRDL
ETAQIAIRSALTGHLVFSTLHTNNAIATLSRLVDMGIPPFLVSSAVEGVLSQRLVRIICSNCKIEYSPTAEEQEIYHRYS
GEQVDTLYKGKGCTNCNNTGYKGRTAIHELLILDKTLKDMLAREASERELTEEARKRGFSYLIENAISKVSQGITTMEEV
IRATFHQESHL

Sequences:

>Translated_571_residues
MFRQKKQRLGDLLLESGAITEEDLKQALDHQNKSGQKLGASLVDLGIITEEEIIEVLEFQLGIPHVSLSQYDTNRETATL
IPAYLAERYQVLPIDNRSGKLVLAMGDPLNVVAIDDVKMATGMEVEPVIASPREIEGEINRHFGIQDSVEKAIEEIEGSA
EEEAESEIAATEEEELSNLETNAPVVKVVNSLVSQAYEQGASDIHIEPTKQGMQIRYRIDGVLHNVATPPRYAKDLLISR
VKIMAGMDITKKRIPQDGRSNYNIGGHEIDLRVSTLPTIYGEKVVIRLLHKDKVIFSLDKLGFQQDNFKLYQGLLKNSAG
MVLVTGPTGCGKTTTLYSSLNRINSSEKNIITIEDPVEYQIEGINQVQTNEKGGLTFANGLRAILRQDPDIIMVGEIRDL
ETAQIAIRSALTGHLVFSTLHTNNAIATLSRLVDMGIPPFLVSSAVEGVLSQRLVRIICSNCKIEYSPTAEEQEIYHRYS
GEQVDTLYKGKGCTNCNNTGYKGRTAIHELLILDKTLKDMLAREASERELTEEARKRGFSYLIENAISKVSQGITTMEEV
IRATFHQESHL
>Mature_571_residues
MFRQKKQRLGDLLLESGAITEEDLKQALDHQNKSGQKLGASLVDLGIITEEEIIEVLEFQLGIPHVSLSQYDTNRETATL
IPAYLAERYQVLPIDNRSGKLVLAMGDPLNVVAIDDVKMATGMEVEPVIASPREIEGEINRHFGIQDSVEKAIEEIEGSA
EEEAESEIAATEEEELSNLETNAPVVKVVNSLVSQAYEQGASDIHIEPTKQGMQIRYRIDGVLHNVATPPRYAKDLLISR
VKIMAGMDITKKRIPQDGRSNYNIGGHEIDLRVSTLPTIYGEKVVIRLLHKDKVIFSLDKLGFQQDNFKLYQGLLKNSAG
MVLVTGPTGCGKTTTLYSSLNRINSSEKNIITIEDPVEYQIEGINQVQTNEKGGLTFANGLRAILRQDPDIIMVGEIRDL
ETAQIAIRSALTGHLVFSTLHTNNAIATLSRLVDMGIPPFLVSSAVEGVLSQRLVRIICSNCKIEYSPTAEEQEIYHRYS
GEQVDTLYKGKGCTNCNNTGYKGRTAIHELLILDKTLKDMLAREASERELTEEARKRGFSYLIENAISKVSQGITTMEEV
IRATFHQESHL

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=399, Percent_Identity=47.1177944862155, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1786296, Length=426, Percent_Identity=40.1408450704225, Blast_Score=319, Evalue=4e-88,
Organism=Escherichia coli, GI87082188, Length=248, Percent_Identity=34.2741935483871, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 63402; Mature: 63402

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRQKKQRLGDLLLESGAITEEDLKQALDHQNKSGQKLGASLVDLGIITEEEIIEVLEFQ
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LGIPHVSLSQYDTNRETATLIPAYLAERYQVLPIDNRSGKLVLAMGDPLNVVAIDDVKMA
HCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEEEECCEEHH
TGMEVEPVIASPREIEGEINRHFGIQDSVEKAIEEIEGSAEEEAESEIAATEEEELSNLE
CCCCCCCCCCCCHHHCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCC
TNAPVVKVVNSLVSQAYEQGASDIHIEPTKQGMQIRYRIDGVLHNVATPPRYAKDLLISR
CCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHH
VKIMAGMDITKKRIPQDGRSNYNIGGHEIDLRVSTLPTIYGEKVVIRLLHKDKVIFSLDK
HHHHHCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHCCHHHHHHHHCCCCEEEHHH
LGFQQDNFKLYQGLLKNSAGMVLVTGPTGCGKTTTLYSSLNRINSSEKNIITIEDPVEYQ
HCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEE
IEGINQVQTNEKGGLTFANGLRAILRQDPDIIMVGEIRDLETAQIAIRSALTGHLVFSTL
ECCCCHHCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEEH
HTNNAIATLSRLVDMGIPPFLVSSAVEGVLSQRLVRIICSNCKIEYSPTAEEQEIYHRYS
HCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHC
GEQVDTLYKGKGCTNCNNTGYKGRTAIHELLILDKTLKDMLAREASERELTEEARKRGFS
CCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
YLIENAISKVSQGITTMEEVIRATFHQESHL
HHHHHHHHHHHHCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFRQKKQRLGDLLLESGAITEEDLKQALDHQNKSGQKLGASLVDLGIITEEEIIEVLEFQ
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LGIPHVSLSQYDTNRETATLIPAYLAERYQVLPIDNRSGKLVLAMGDPLNVVAIDDVKMA
HCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEEEECCEEHH
TGMEVEPVIASPREIEGEINRHFGIQDSVEKAIEEIEGSAEEEAESEIAATEEEELSNLE
CCCCCCCCCCCCHHHCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCC
TNAPVVKVVNSLVSQAYEQGASDIHIEPTKQGMQIRYRIDGVLHNVATPPRYAKDLLISR
CCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHH
VKIMAGMDITKKRIPQDGRSNYNIGGHEIDLRVSTLPTIYGEKVVIRLLHKDKVIFSLDK
HHHHHCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHCCHHHHHHHHCCCCEEEHHH
LGFQQDNFKLYQGLLKNSAGMVLVTGPTGCGKTTTLYSSLNRINSSEKNIITIEDPVEYQ
HCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCEE
IEGINQVQTNEKGGLTFANGLRAILRQDPDIIMVGEIRDLETAQIAIRSALTGHLVFSTL
ECCCCHHCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHEEEH
HTNNAIATLSRLVDMGIPPFLVSSAVEGVLSQRLVRIICSNCKIEYSPTAEEQEIYHRYS
HCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHC
GEQVDTLYKGKGCTNCNNTGYKGRTAIHELLILDKTLKDMLAREASERELTEEARKRGFS
CCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
YLIENAISKVSQGITTMEEVIRATFHQESHL
HHHHHHHHHHHHCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]