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The map label for this gene is pilT [H]

Identifier: 188586712

GI number: 188586712

Start: 2218111

End: 2219157

Strand: Reverse

Name: pilT [H]

Synonym: Nther_2102

Alternate gene names: 188586712

Gene position: 2219157-2218111 (Counterclockwise)

Preceding gene: 188586713

Following gene: 188586711

Centisome position: 70.1

GC content: 41.26

Gene sequence:

>1047_bases
TTGAATATAACTATAGATGAGCTGTTATCTCAGGCGGTTTCTCAAGGGGCCTCTGATCTGCACGTAACAGTTGAATCCCC
TCCTATAATACGTAAAGATGGAGAACTTTTTCCCCTGTCATATGATAAATTCAGTCCCGAATTGACTGAAAAGATGGCCA
AGGAAGTTCTGGCTGTCAAGGGAATAGATGCCTTAACATCGGAATTAGGTGAAACAGATCTATCCTATAGCATACCGGGT
ACTGGTAGGTTTAGAATCAATGTCTTTCTGCAGAGAAATACGTACGGAATTTCTTGTCGTGTTATTTCTTCTCGGGTTCC
CAATCTAGAGGAATTAGGCCTCCCTCCTATTGTAAAGAGTCTTGCCCAGGAAAATAGGGGTTTAGTAGTGGTTACCGGGG
CTACCGGTAGCGGTAAATCTACGACTTTAGCTGCCATGATAGATGAAATCAATAAAAACCGAACTTGTCATGTTGTTACT
CTGGAAGATCCTATTGAATATTTGCATAATCACAATCAAAGTATAATCAATCAACGGGAAATCGGTAGTGATTCAGCTGG
TTACGCTGGGGCTTTGAGAGCGGCTTTACGACAAGATCCCGATGTAATCCTTGTTGGAGAGATGCGGGACAGGGAAACTA
TCCAAACTGCCATTACGGCAGCGGAAACTGGTCATTTAGTATTGACAACTTTACATACAGTAGATGCCCCTCAAACCATT
GATAGAATCGTGGATGTTTTTCCACCACATCAACAGAATCAAATTAGAGTTCAATTGGCAGGTACTCTGAAAGGCATTTT
GGCACAACAACTGCTTCCCAAGTCACAAGGCGGCCGCGTTGCTGCATTGGAGTGTCTTGTTTCGACTTCTGGTATTAAAA
ATTTAATTAGGGAAGGTAAAACATATCAGATCTATTCTCAAATGCAAACTGGGGCAAAATTAGGCATGCAGACTATGGAA
AGTGCAGTTAAAGATTTGTTTCAAAATGGACTTATAACAGAAGCTACTATGGAAAAAAGTTTACCAGCCAAGGGAAAGCT
AAAATAA

Upstream 100 bases:

>100_bases
TGAATAATATTGATTCATCGACTAATAATTGAATTTATCGATAATTATTGAATCACTGCGCAGATTTGATTTATGTCTTC
CAAGAGGAGAGTGATCCAAA

Downstream 100 bases:

>100_bases
AATCAGCCAAGGTGAACTTAAAATTAGTCTCTTGTATATTCCGTTGTTGTTGAACTCTTGGATTGAACGAATTCTGTAGG
GTTGATATTACATATTTAAC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNITIDELLSQAVSQGASDLHVTVESPPIIRKDGELFPLSYDKFSPELTEKMAKEVLAVKGIDALTSELGETDLSYSIPG
TGRFRINVFLQRNTYGISCRVISSRVPNLEELGLPPIVKSLAQENRGLVVVTGATGSGKSTTLAAMIDEINKNRTCHVVT
LEDPIEYLHNHNQSIINQREIGSDSAGYAGALRAALRQDPDVILVGEMRDRETIQTAITAAETGHLVLTTLHTVDAPQTI
DRIVDVFPPHQQNQIRVQLAGTLKGILAQQLLPKSQGGRVAALECLVSTSGIKNLIREGKTYQIYSQMQTGAKLGMQTME
SAVKDLFQNGLITEATMEKSLPAKGKLK

Sequences:

>Translated_348_residues
MNITIDELLSQAVSQGASDLHVTVESPPIIRKDGELFPLSYDKFSPELTEKMAKEVLAVKGIDALTSELGETDLSYSIPG
TGRFRINVFLQRNTYGISCRVISSRVPNLEELGLPPIVKSLAQENRGLVVVTGATGSGKSTTLAAMIDEINKNRTCHVVT
LEDPIEYLHNHNQSIINQREIGSDSAGYAGALRAALRQDPDVILVGEMRDRETIQTAITAAETGHLVLTTLHTVDAPQTI
DRIVDVFPPHQQNQIRVQLAGTLKGILAQQLLPKSQGGRVAALECLVSTSGIKNLIREGKTYQIYSQMQTGAKLGMQTME
SAVKDLFQNGLITEATMEKSLPAKGKLK
>Mature_348_residues
MNITIDELLSQAVSQGASDLHVTVESPPIIRKDGELFPLSYDKFSPELTEKMAKEVLAVKGIDALTSELGETDLSYSIPG
TGRFRINVFLQRNTYGISCRVISSRVPNLEELGLPPIVKSLAQENRGLVVVTGATGSGKSTTLAAMIDEINKNRTCHVVT
LEDPIEYLHNHNQSIINQREIGSDSAGYAGALRAALRQDPDVILVGEMRDRETIQTAITAAETGHLVLTTLHTVDAPQTI
DRIVDVFPPHQQNQIRVQLAGTLKGILAQQLLPKSQGGRVAALECLVSTSGIKNLIREGKTYQIYSQMQTGAKLGMQTME
SAVKDLFQNGLITEATMEKSLPAKGKLK

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=322, Percent_Identity=43.7888198757764, Blast_Score=271, Evalue=3e-74,
Organism=Escherichia coli, GI1786296, Length=281, Percent_Identity=33.4519572953737, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789723, Length=286, Percent_Identity=32.8671328671329, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 37811; Mature: 37811

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITIDELLSQAVSQGASDLHVTVESPPIIRKDGELFPLSYDKFSPELTEKMAKEVLAVK
CCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCEECCCCHHCCHHHHHHHHHHHHHHC
GIDALTSELGETDLSYSIPGTGRFRINVFLQRNTYGISCRVISSRVPNLEELGLPPIVKS
CHHHHHHHHCCCCCEEECCCCCEEEEEEEEEECCCEEEEEEECCCCCCHHHCCCCHHHHH
LAQENRGLVVVTGATGSGKSTTLAAMIDEINKNRTCHVVTLEDPIEYLHNHNQSIINQRE
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCHHHHHHHH
IGSDSAGYAGALRAALRQDPDVILVGEMRDRETIQTAITAAETGHLVLTTLHTVDAPQTI
CCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
DRIVDVFPPHQQNQIRVQLAGTLKGILAQQLLPKSQGGRVAALECLVSTSGIKNLIREGK
HHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCC
TYQIYSQMQTGAKLGMQTMESAVKDLFQNGLITEATMEKSLPAKGKLK
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNITIDELLSQAVSQGASDLHVTVESPPIIRKDGELFPLSYDKFSPELTEKMAKEVLAVK
CCCCHHHHHHHHHHCCCCEEEEEECCCCCEECCCCEECCCCHHCCHHHHHHHHHHHHHHC
GIDALTSELGETDLSYSIPGTGRFRINVFLQRNTYGISCRVISSRVPNLEELGLPPIVKS
CHHHHHHHHCCCCCEEECCCCCEEEEEEEEEECCCEEEEEEECCCCCCHHHCCCCHHHHH
LAQENRGLVVVTGATGSGKSTTLAAMIDEINKNRTCHVVTLEDPIEYLHNHNQSIINQRE
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHCCCHHHHHHHH
IGSDSAGYAGALRAALRQDPDVILVGEMRDRETIQTAITAAETGHLVLTTLHTVDAPQTI
CCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHH
DRIVDVFPPHQQNQIRVQLAGTLKGILAQQLLPKSQGGRVAALECLVSTSGIKNLIREGK
HHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCC
TYQIYSQMQTGAKLGMQTMESAVKDLFQNGLITEATMEKSLPAKGKLK
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]