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The map label for this gene is liaS [H]

Identifier: 188586690

GI number: 188586690

Start: 2199751

End: 2200863

Strand: Reverse

Name: liaS [H]

Synonym: Nther_2080

Alternate gene names: 188586690

Gene position: 2200863-2199751 (Counterclockwise)

Preceding gene: 188586691

Following gene: 188586689

Centisome position: 69.53

GC content: 38.36

Gene sequence:

>1113_bases
ATGCCGTTGATTAAGAAGTTAATCACTAAATTGAGGCCAAAGGGGATTTTATGGGAATTTTTTCAGCGGCAATTAGTGGG
TGCCCTGGCGGCAGGTTTAATTATATTTTTTTGTATTTATTTAATATGGCCGGGAGATATGGATCTTGAGATTGCTACTG
CCATTGTATTGGGTTCGCCAGTAGTAGTTTTTCTTAGTTCTTGGGTATCTTGGTATAAATATCTCAGAAATTTAAAACAA
TCAATTAACTATATGGGCAGATATATATTGGAACTAAAACGAGGCAATCTTAATAATCGCCTTGATATTCACAGGGACGA
TGAGATTCAGGATTTAGGTGAACGCTTGGAAGAGTTAACCAAAGATTATAAGCAACAAGTTAGGGCTAGTCAAAAAAAGA
TTAATGAAAATAAAGATTTAATTGCCCGGGCGGAAGAAGCTGCTAGCTTAGAAGAAAGGCGTAAATTGGCTAGAGAACTG
CATGATGCTGTCAGTCAGCAGCTTTTTGCAGCTTCTATGTCAATGAAAGCCATGGATAAAATGATTTATCAGGATCCTGA
TAAGGCAAGACAGCTATTTTCGAAAACTACTTCCATGATCCAAAATGCCCAACAGGAACTACGGGCTTTAATATTACATT
TGAGACCTGTTACATTAGAAGGCAAAAGCCTATCTGAAGGTTTGGAACAGTTGCTGCAAGAGATTAAGGATAAACACAAA
AACTTTAATATTTCCTGGGAAATCACTCAACTTCCAGAACTTGCTTCTGGGGTGGAAGACCAGGTATTTCGAGTGATTCA
GGAAGCCATCTCCAATATGCTCCGTCACTCACAAGCTAAAAATTTTATTGTGAATGGAAAATATAAAGGGAATCGGATTT
ATATTTCTTTGGAAGATGATGGTGTAGGTTTTAATTTAAGCCAGGCTTTGAACAAGCAGTCTTCTTCCTATGGTTTGAAA
ACCATGAAGGAACGTATGGTTGAGTTAGGGGGACATCTAAACATCCTTACCTATCCAGGATCGGGGACCCGAGTTGAGCT
GAGATTACCTATAACATCTTCAGAAAAAGAAAGCGGTGAAAACGACAGTAATGGGAGGGAAAGCCATGTATAA

Upstream 100 bases:

>100_bases
GGTATGAGTCGTCCTGTAATTTATACTAGTCCAAATTATCAGGATGCAGAAAGAAAATTAAAAATTATTGTAATGCTCTC
CGTTGGAGAGGTGGGCGTTT

Downstream 100 bases:

>100_bases
ACAAGAGGGTGAAAAAATATCTGTTTTAATTGTGGATGATCATGAAATGGTCAGATTGGGACTTCGCAGTTACCTGGAGA
CCCAGGAGGATATTGAAGTT

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MPLIKKLITKLRPKGILWEFFQRQLVGALAAGLIIFFCIYLIWPGDMDLEIATAIVLGSPVVVFLSSWVSWYKYLRNLKQ
SINYMGRYILELKRGNLNNRLDIHRDDEIQDLGERLEELTKDYKQQVRASQKKINENKDLIARAEEAASLEERRKLAREL
HDAVSQQLFAASMSMKAMDKMIYQDPDKARQLFSKTTSMIQNAQQELRALILHLRPVTLEGKSLSEGLEQLLQEIKDKHK
NFNISWEITQLPELASGVEDQVFRVIQEAISNMLRHSQAKNFIVNGKYKGNRIYISLEDDGVGFNLSQALNKQSSSYGLK
TMKERMVELGGHLNILTYPGSGTRVELRLPITSSEKESGENDSNGRESHV

Sequences:

>Translated_370_residues
MPLIKKLITKLRPKGILWEFFQRQLVGALAAGLIIFFCIYLIWPGDMDLEIATAIVLGSPVVVFLSSWVSWYKYLRNLKQ
SINYMGRYILELKRGNLNNRLDIHRDDEIQDLGERLEELTKDYKQQVRASQKKINENKDLIARAEEAASLEERRKLAREL
HDAVSQQLFAASMSMKAMDKMIYQDPDKARQLFSKTTSMIQNAQQELRALILHLRPVTLEGKSLSEGLEQLLQEIKDKHK
NFNISWEITQLPELASGVEDQVFRVIQEAISNMLRHSQAKNFIVNGKYKGNRIYISLEDDGVGFNLSQALNKQSSSYGLK
TMKERMVELGGHLNILTYPGSGTRVELRLPITSSEKESGENDSNGRESHV
>Mature_369_residues
PLIKKLITKLRPKGILWEFFQRQLVGALAAGLIIFFCIYLIWPGDMDLEIATAIVLGSPVVVFLSSWVSWYKYLRNLKQS
INYMGRYILELKRGNLNNRLDIHRDDEIQDLGERLEELTKDYKQQVRASQKKINENKDLIARAEEAASLEERRKLARELH
DAVSQQLFAASMSMKAMDKMIYQDPDKARQLFSKTTSMIQNAQQELRALILHLRPVTLEGKSLSEGLEQLLQEIKDKHKN
FNISWEITQLPELASGVEDQVFRVIQEAISNMLRHSQAKNFIVNGKYKGNRIYISLEDDGVGFNLSQALNKQSSSYGLKT
MKERMVELGGHLNILTYPGSGTRVELRLPITSSEKESGENDSNGRESHV

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=206, Percent_Identity=29.126213592233, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787474, Length=204, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082311, Length=241, Percent_Identity=28.2157676348548, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42370; Mature: 42239

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLIKKLITKLRPKGILWEFFQRQLVGALAAGLIIFFCIYLIWPGDMDLEIATAIVLGSP
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCH
VVVFLSSWVSWYKYLRNLKQSINYMGRYILELKRGNLNNRLDIHRDDEIQDLGERLEELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
KDYKQQVRASQKKINENKDLIARAEEAASLEERRKLARELHDAVSQQLFAASMSMKAMDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIYQDPDKARQLFSKTTSMIQNAQQELRALILHLRPVTLEGKSLSEGLEQLLQEIKDKHK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHC
NFNISWEITQLPELASGVEDQVFRVIQEAISNMLRHSQAKNFIVNGKYKGNRIYISLEDD
CCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECEEECCEEEEEEECC
GVGFNLSQALNKQSSSYGLKTMKERMVELGGHLNILTYPGSGTRVELRLPITSSEKESGE
CCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCC
NDSNGRESHV
CCCCCCCCCC
>Mature Secondary Structure 
PLIKKLITKLRPKGILWEFFQRQLVGALAAGLIIFFCIYLIWPGDMDLEIATAIVLGSP
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCH
VVVFLSSWVSWYKYLRNLKQSINYMGRYILELKRGNLNNRLDIHRDDEIQDLGERLEELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
KDYKQQVRASQKKINENKDLIARAEEAASLEERRKLARELHDAVSQQLFAASMSMKAMDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIYQDPDKARQLFSKTTSMIQNAQQELRALILHLRPVTLEGKSLSEGLEQLLQEIKDKHK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHC
NFNISWEITQLPELASGVEDQVFRVIQEAISNMLRHSQAKNFIVNGKYKGNRIYISLEDD
CCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECEEECCEEEEEEECC
GVGFNLSQALNKQSSSYGLKTMKERMVELGGHLNILTYPGSGTRVELRLPITSSEKESGE
CCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCC
NDSNGRESHV
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]