Click here to switch to the map view.

The map label for this gene is liaR [H]

Identifier: 188586689

GI number: 188586689

Start: 2199120

End: 2199758

Strand: Reverse

Name: liaR [H]

Synonym: Nther_2079

Alternate gene names: 188586689

Gene position: 2199758-2199120 (Counterclockwise)

Preceding gene: 188586690

Following gene: 188586687

Centisome position: 69.49

GC content: 34.9

Gene sequence:

>639_bases
ATGTATAAACAAGAGGGTGAAAAAATATCTGTTTTAATTGTGGATGATCATGAAATGGTCAGATTGGGACTTCGCAGTTA
CCTGGAGACCCAGGAGGATATTGAAGTTGTAGCGGAAGCTTGTGATGGACAAGAAGGTATTGATTTGGCAAGGGAATACC
AACCTCAAGTTATTTTAATGGATTTAGTTATGGATGGCATGGATGGAATCCAGGCTACCAGGGAATTACAGGGAGAAGGA
TTTAAAATTGTTGTTCTTACAAGTTATGTAGATGATGATAAAGTATTTCCCGCTTTAGAAGCTGGAGCTTATAGTTATAT
ACTAAAAACTTCTGATGCGGAAGATATTGCCAAATGTATTCGAAAAGCAAGTGAAGGAAAACCCAGTTTTGAGGGTCAAG
TTACTAAATTAATGATGGAACGTAGGGAAAATGAATCGGCATATCTTAACTTAACTAATAGAGAAAAAGAAGTTTTGAGA
TTACTGGGTAAAGGGTTTACTAATCAGGAAATAGCCGATGAATTATATATTGGTATAAAAACAGTCAAGACACATGTAAG
CAATATTTTAGATAAATTGGAACTGAAAGATAGAACTCAGGCTGCCATTTATGCAAAAGATTATGGTTATACTAATTAA

Upstream 100 bases:

>100_bases
CTTACCTATCCAGGATCGGGGACCCGAGTTGAGCTGAGATTACCTATAACATCTTCAGAAAAAGAAAGCGGTGAAAACGA
CAGTAATGGGAGGGAAAGCC

Downstream 100 bases:

>100_bases
AGATTTGATGAATCTGCAATTGGCCCAAAGGTGTAATGTGTATATACACTTCCTTGAGACAAATACTATGGTTAAATGAA
AGCTGTATTAAAGTTGAATG

Product: two component transcriptional regulator, LuxR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MYKQEGEKISVLIVDDHEMVRLGLRSYLETQEDIEVVAEACDGQEGIDLAREYQPQVILMDLVMDGMDGIQATRELQGEG
FKIVVLTSYVDDDKVFPALEAGAYSYILKTSDAEDIAKCIRKASEGKPSFEGQVTKLMMERRENESAYLNLTNREKEVLR
LLGKGFTNQEIADELYIGIKTVKTHVSNILDKLELKDRTQAAIYAKDYGYTN

Sequences:

>Translated_212_residues
MYKQEGEKISVLIVDDHEMVRLGLRSYLETQEDIEVVAEACDGQEGIDLAREYQPQVILMDLVMDGMDGIQATRELQGEG
FKIVVLTSYVDDDKVFPALEAGAYSYILKTSDAEDIAKCIRKASEGKPSFEGQVTKLMMERRENESAYLNLTNREKEVLR
LLGKGFTNQEIADELYIGIKTVKTHVSNILDKLELKDRTQAAIYAKDYGYTN
>Mature_212_residues
MYKQEGEKISVLIVDDHEMVRLGLRSYLETQEDIEVVAEACDGQEGIDLAREYQPQVILMDLVMDGMDGIQATRELQGEG
FKIVVLTSYVDDDKVFPALEAGAYSYILKTSDAEDIAKCIRKASEGKPSFEGQVTKLMMERRENESAYLNLTNREKEVLR
LLGKGFTNQEIADELYIGIKTVKTHVSNILDKLELKDRTQAAIYAKDYGYTN

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=204, Percent_Identity=39.7058823529412, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=30, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1787473, Length=205, Percent_Identity=35.1219512195122, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1786747, Length=203, Percent_Identity=30.0492610837438, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788712, Length=190, Percent_Identity=24.7368421052632, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1790102, Length=194, Percent_Identity=27.319587628866, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788546, Length=190, Percent_Identity=28.9473684210526, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI87082052, Length=115, Percent_Identity=34.7826086956522, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787277, Length=162, Percent_Identity=30.2469135802469, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1788394, Length=190, Percent_Identity=27.3684210526316, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23976; Mature: 23976

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKQEGEKISVLIVDDHEMVRLGLRSYLETQEDIEVVAEACDGQEGIDLAREYQPQVILM
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEEH
DLVMDGMDGIQATRELQGEGFKIVVLTSYVDDDKVFPALEAGAYSYILKTSDAEDIAKCI
HHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHCCCEEEEEECCCHHHHHHHH
RKASEGKPSFEGQVTKLMMERRENESAYLNLTNREKEVLRLLGKGFTNQEIADELYIGIK
HHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHH
TVKTHVSNILDKLELKDRTQAAIYAKDYGYTN
HHHHHHHHHHHHHHCCCCHHEEEEECCCCCCC
>Mature Secondary Structure
MYKQEGEKISVLIVDDHEMVRLGLRSYLETQEDIEVVAEACDGQEGIDLAREYQPQVILM
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEEH
DLVMDGMDGIQATRELQGEGFKIVVLTSYVDDDKVFPALEAGAYSYILKTSDAEDIAKCI
HHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHCCCEEEEEECCCHHHHHHHH
RKASEGKPSFEGQVTKLMMERRENESAYLNLTNREKEVLRLLGKGFTNQEIADELYIGIK
HHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHH
TVKTHVSNILDKLELKDRTQAAIYAKDYGYTN
HHHHHHHHHHHHHHCCCCHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]