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The map label for this gene is thiE [H]

Identifier: 188586532

GI number: 188586532

Start: 2013396

End: 2014034

Strand: Direct

Name: thiE [H]

Synonym: Nther_1918

Alternate gene names: 188586532

Gene position: 2013396-2014034 (Clockwise)

Preceding gene: 188586531

Following gene: 188586539

Centisome position: 63.6

GC content: 37.87

Gene sequence:

>639_bases
ATGACTACTGGAAACAAAGGATTAAATACAGATATTTATGCTATTACGGCCGAACCCTTTTCCAGGGGAAGAGATAATAT
TGCAGTGGTCAGTAAAATGCTCAAAGCCGGAATAAAAACCATTCAGTACAGGGAAAAAGATAAAACACCCAGAGAAAAGC
TTACACAGTGCAAAAAGATCAGGGAAATGACCAGAGAAGCAAATTGTAAACTGATTATTAATGATGATATAGATATCGCA
CTACTAGTTGGTGCAGACGGAGTTCACGTCGGTCAAGATGATCTACCAGTTAATCAGGTAAGGAAATTAGTGGGTGATGA
TATGATTATTGGACTTTCAACCCATTCTCCCGATCAAGCCAAGGAAGCGATTTCATTAGGTGCCGACTATATTGGAGTAG
GGCCTGTATTTGAGACAAATACAAAGGAAGATGTTCAAAAACCAGTAGGACTTGAATATGTTGATTACGTTTCGACTAAC
CTGAATATCCCTTTTACTGCCATTGGTGGTATTAAACGCAACAATATTCATTTAGTTAAGGAAAAGGGAGCTTCATGCTT
TGCCATTGTTACCGAGATAGTTGAAGCCCCAAATATTCCTGAAAGAATTCATGAGATTCGATCAATTATTCATTCATAA

Upstream 100 bases:

>100_bases
GAACGTTCAGTAGTAGAGATCAAAGGTATGTTAATAAATAATGGTTATCAACCGGTATTAAAAGACTGGGAATTAATTTA
GATTAGGGAGGGTTAATTTA

Downstream 100 bases:

>100_bases
ACAAGAACAGCAAATAACATCCAGGTATTAGTCCAAATAGCGCCAATATCAAAGGCAGAGCAGCTGGCACTGTATTAACA
AAATTATCACAGGACCAGCT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTTGNKGLNTDIYAITAEPFSRGRDNIAVVSKMLKAGIKTIQYREKDKTPREKLTQCKKIREMTREANCKLIINDDIDIA
LLVGADGVHVGQDDLPVNQVRKLVGDDMIIGLSTHSPDQAKEAISLGADYIGVGPVFETNTKEDVQKPVGLEYVDYVSTN
LNIPFTAIGGIKRNNIHLVKEKGASCFAIVTEIVEAPNIPERIHEIRSIIHS

Sequences:

>Translated_212_residues
MTTGNKGLNTDIYAITAEPFSRGRDNIAVVSKMLKAGIKTIQYREKDKTPREKLTQCKKIREMTREANCKLIINDDIDIA
LLVGADGVHVGQDDLPVNQVRKLVGDDMIIGLSTHSPDQAKEAISLGADYIGVGPVFETNTKEDVQKPVGLEYVDYVSTN
LNIPFTAIGGIKRNNIHLVKEKGASCFAIVTEIVEAPNIPERIHEIRSIIHS
>Mature_211_residues
TTGNKGLNTDIYAITAEPFSRGRDNIAVVSKMLKAGIKTIQYREKDKTPREKLTQCKKIREMTREANCKLIINDDIDIAL
LVGADGVHVGQDDLPVNQVRKLVGDDMIIGLSTHSPDQAKEAISLGADYIGVGPVFETNTKEDVQKPVGLEYVDYVSTNL
NIPFTAIGGIKRNNIHLVKEKGASCFAIVTEIVEAPNIPERIHEIRSIIHS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=185, Percent_Identity=31.3513513513514, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=198, Percent_Identity=32.8282828282828, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23322; Mature: 23191

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGNKGLNTDIYAITAEPFSRGRDNIAVVSKMLKAGIKTIQYREKDKTPREKLTQCKKI
CCCCCCCCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
REMTREANCKLIINDDIDIALLVGADGVHVGQDDLPVNQVRKLVGDDMIIGLSTHSPDQA
HHHHHCCCCEEEEECCCCEEEEECCCCCEECCCCCCHHHHHHHHCCCEEEEECCCCCHHH
KEAISLGADYIGVGPVFETNTKEDVQKPVGLEYVDYVSTNLNIPFTAIGGIKRNNIHLVK
HHHHHHCCCEEECCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCEEEECCEECCCEEEEE
EKGASCFAIVTEIVEAPNIPERIHEIRSIIHS
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TTGNKGLNTDIYAITAEPFSRGRDNIAVVSKMLKAGIKTIQYREKDKTPREKLTQCKKI
CCCCCCCCCCEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
REMTREANCKLIINDDIDIALLVGADGVHVGQDDLPVNQVRKLVGDDMIIGLSTHSPDQA
HHHHHCCCCEEEEECCCCEEEEECCCCCEECCCCCCHHHHHHHHCCCEEEEECCCCCHHH
KEAISLGADYIGVGPVFETNTKEDVQKPVGLEYVDYVSTNLNIPFTAIGGIKRNNIHLVK
HHHHHHCCCEEECCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCCEEEECCEECCCEEEEE
EKGASCFAIVTEIVEAPNIPERIHEIRSIIHS
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA