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The map label for this gene is thiH [H]

Identifier: 188586531

GI number: 188586531

Start: 2012273

End: 2013376

Strand: Direct

Name: thiH [H]

Synonym: Nther_1917

Alternate gene names: 188586531

Gene position: 2012273-2013376 (Clockwise)

Preceding gene: 188586530

Following gene: 188586532

Centisome position: 63.57

GC content: 36.68

Gene sequence:

>1104_bases
ATGAGTTTTAGAGATTTTATTAAAAAATACCAACAGCTAGACTTTCAGCAAACTTTTCAAGATATCACTCCCCAAAGGGT
TGAAACCGCAATTTATAAAGACAATCCAAACTTTAGAGACTTTTTAGCCATGCTATCGCCAGCCGCAGAAAATTACCTGG
AAGAAATGGCGCAAAAAGCTAATCAACTCACCACAAATTTTTTTGGAAAAGCTATCGTTTTGTATGCTCCCATTTACGTT
AGTGATCATTGTGATAATAACTGCCTTTATTGTGCTTTTAAAGTAGATAATCAGTTTCAAAGAACTACTTTAAGCCTTGA
AGAAGTTGAACAAGAAGCCCAAGCTATCAGCCATACTGGTCTTCGCCATATATTGCTTTTAACTGGTGAATCTAAACCAC
ATGCACCCCTAGATTATATAGAAAAATGTATTGATATTTTAAAAAAATACTTTTCTTCTATAGCTATAGAAATCTATCCA
TTGACAGCCAAAGAATATAAACAGTTAATTGACAATGAAGTTGATGGACTTACAATCTACCAAGAGGTTTACGACGAAGA
TATTTATCAACAAGTTCATAAAAGCGGCCCAAAACGCGATTATGATTTTCGATTACTAGCCCCGGAACGCAGCCTACAAA
TGGGCATGAGAAGGGTTAACATAGGTGCATTATTTGGTTTGGGCCCCTGGAGGCAGGAAGCTTTTTTTACTGGTCTACAT
GCTTGGTACTTATTAAATCATTATCCGGAAGCTGAAATTTCCATATCTTTTCCCCGCTTAAGACCTTTTGCTGATGAAAC
CTTGGAATATTATAGAGTTGCGGATAAAAACTTGGTTCAAATGATAGTAGCCACAAGAATTTTTTTACACAGTGTGGGGA
TCAATATTTCTACTAGAGAAAGCCCTGATTTAAGAGAAAATCTGCTTCCCCTAGGAGTAACTAGAATGTCGGCAGGAGCT
AAGACAGCTGTAGGAAGCTATTCCGGTGTAGAAAATAGCGAGTCTCAGTTTCATACCGCCGATGAACGTTCAGTAGTAGA
GATCAAAGGTATGTTAATAAATAATGGTTATCAACCGGTATTAAAAGACTGGGAATTAATTTAG

Upstream 100 bases:

>100_bases
GAAAGCATATTTATCCAAAGCCGCACCCGTGAAAGAGACTGCTGAAGCTTCTTCACCTTTGACTGGTTTTCTTCGAGACG
AAAATGGGGAGGGATAAGTA

Downstream 100 bases:

>100_bases
ATTAGGGAGGGTTAATTTAATGACTACTGGAAACAAAGGATTAAATACAGATATTTATGCTATTACGGCCGAACCCTTTT
CCAGGGGAAGAGATAATATT

Product: thiazole biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSFRDFIKKYQQLDFQQTFQDITPQRVETAIYKDNPNFRDFLAMLSPAAENYLEEMAQKANQLTTNFFGKAIVLYAPIYV
SDHCDNNCLYCAFKVDNQFQRTTLSLEEVEQEAQAISHTGLRHILLLTGESKPHAPLDYIEKCIDILKKYFSSIAIEIYP
LTAKEYKQLIDNEVDGLTIYQEVYDEDIYQQVHKSGPKRDYDFRLLAPERSLQMGMRRVNIGALFGLGPWRQEAFFTGLH
AWYLLNHYPEAEISISFPRLRPFADETLEYYRVADKNLVQMIVATRIFLHSVGINISTRESPDLRENLLPLGVTRMSAGA
KTAVGSYSGVENSESQFHTADERSVVEIKGMLINNGYQPVLKDWELI

Sequences:

>Translated_367_residues
MSFRDFIKKYQQLDFQQTFQDITPQRVETAIYKDNPNFRDFLAMLSPAAENYLEEMAQKANQLTTNFFGKAIVLYAPIYV
SDHCDNNCLYCAFKVDNQFQRTTLSLEEVEQEAQAISHTGLRHILLLTGESKPHAPLDYIEKCIDILKKYFSSIAIEIYP
LTAKEYKQLIDNEVDGLTIYQEVYDEDIYQQVHKSGPKRDYDFRLLAPERSLQMGMRRVNIGALFGLGPWRQEAFFTGLH
AWYLLNHYPEAEISISFPRLRPFADETLEYYRVADKNLVQMIVATRIFLHSVGINISTRESPDLRENLLPLGVTRMSAGA
KTAVGSYSGVENSESQFHTADERSVVEIKGMLINNGYQPVLKDWELI
>Mature_366_residues
SFRDFIKKYQQLDFQQTFQDITPQRVETAIYKDNPNFRDFLAMLSPAAENYLEEMAQKANQLTTNFFGKAIVLYAPIYVS
DHCDNNCLYCAFKVDNQFQRTTLSLEEVEQEAQAISHTGLRHILLLTGESKPHAPLDYIEKCIDILKKYFSSIAIEIYPL
TAKEYKQLIDNEVDGLTIYQEVYDEDIYQQVHKSGPKRDYDFRLLAPERSLQMGMRRVNIGALFGLGPWRQEAFFTGLHA
WYLLNHYPEAEISISFPRLRPFADETLEYYRVADKNLVQMIVATRIFLHSVGINISTRESPDLRENLLPLGVTRMSAGAK
TAVGSYSGVENSESQFHTADERSVVEIKGMLINNGYQPVLKDWELI

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=367, Percent_Identity=35.6948228882834, Blast_Score=257, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42304; Mature: 42172

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRDFIKKYQQLDFQQTFQDITPQRVETAIYKDNPNFRDFLAMLSPAAENYLEEMAQKA
CCHHHHHHHHHHCCHHHHHHHCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
NQLTTNFFGKAIVLYAPIYVSDHCDNNCLYCAFKVDNQFQRTTLSLEEVEQEAQAISHTG
HHHHHHHHCCEEEEEECEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
LRHILLLTGESKPHAPLDYIEKCIDILKKYFSSIAIEIYPLTAKEYKQLIDNEVDGLTIY
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCCCCCHHH
QEVYDEDIYQQVHKSGPKRDYDFRLLAPERSLQMGMRRVNIGALFGLGPWRQEAFFTGLH
HHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCHHEEECCCCHHHHHHHHHHH
AWYLLNHYPEAEISISFPRLRPFADETLEYYRVADKNLVQMIVATRIFLHSVGINISTRE
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
SPDLRENLLPLGVTRMSAGAKTAVGSYSGVENSESQFHTADERSVVEIKGMLINNGYQPV
CCCHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHH
LKDWELI
HHCCCCC
>Mature Secondary Structure 
SFRDFIKKYQQLDFQQTFQDITPQRVETAIYKDNPNFRDFLAMLSPAAENYLEEMAQKA
CHHHHHHHHHHCCHHHHHHHCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
NQLTTNFFGKAIVLYAPIYVSDHCDNNCLYCAFKVDNQFQRTTLSLEEVEQEAQAISHTG
HHHHHHHHCCEEEEEECEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
LRHILLLTGESKPHAPLDYIEKCIDILKKYFSSIAIEIYPLTAKEYKQLIDNEVDGLTIY
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCCCCCHHH
QEVYDEDIYQQVHKSGPKRDYDFRLLAPERSLQMGMRRVNIGALFGLGPWRQEAFFTGLH
HHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCHHEEECCCCHHHHHHHHHHH
AWYLLNHYPEAEISISFPRLRPFADETLEYYRVADKNLVQMIVATRIFLHSVGINISTRE
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
SPDLRENLLPLGVTRMSAGAKTAVGSYSGVENSESQFHTADERSVVEIKGMLINNGYQPV
CCCHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHH
LKDWELI
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]