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The map label for this gene is nadE [H]

Identifier: 188586416

GI number: 188586416

Start: 1865107

End: 1865859

Strand: Reverse

Name: nadE [H]

Synonym: Nther_1801

Alternate gene names: 188586416

Gene position: 1865859-1865107 (Counterclockwise)

Preceding gene: 188586417

Following gene: 188586415

Centisome position: 58.94

GC content: 37.72

Gene sequence:

>753_bases
TTGTACGTGGACACGCATTCAAAAGCTGAAATGATGGTGGAATCGGTTAGTGATTGGATCAAGCAACAAGTTGAAGAAGC
AGGCTGCAAAGGCACTGTAACTGGACTTAGTGGTGGGATTGATTCTGCGGTAGTGGCCGCTTTATCAAAAAGAGTTTTCC
CTGAAGATACATTAGGTGTGATTATGCCATGTTATAGTGAACCCAGAGATGAAGAGGATGCTTTATTATTTGCTGAACAT
CATAATATTAACGTGAAAAATTTAGACCTATCCGAAACTTACGATTCAATGGTAGGAAAATTGGATGATACAGAGAGCAC
TTCCAAAATGGCTCTTGCTAATATTAAGCCACGACTTAGGATGACTGTATTATATTATTATGCGCAAACCTATAAATATC
TAGTTTTAGGAACTTCTAACAAAAGTGAATTGATTTTAGGGTACTTTACTAAGTATGGTGATGGGGCTGCCGATTTATTA
CCAATAGCTACGTTTACTAAAATGGAAGTGTATGCACTTGCTCGTCAATTAGAGATACCCGAACGCCTGATTAAACGACC
ACCAACAGCAGGTATAATATCAGGCCAGACCGACGAAGATGAGATGGGTTTTACTTACGATGAACTGGAGAAATATTTCA
AAGGGGAACAGATATCTAAAGATGTAAAGAATAAGATTGAAAGACTAGAAAGAATAAATGCACACAAACGTTCTATGCCC
CCTATATATACACCGAAAGAATCATTGAATTAA

Upstream 100 bases:

>100_bases
GGGCTCTTTTGTTTTCGTTGCATACATGTAGAATATGATGTAAAATGGAACATGACTTACTGGAAAAATGTTAAAATGGC
TCGAAAAGGGGTTGACTAGC

Downstream 100 bases:

>100_bases
GTCTTGTAACATTGAATGATTGTTATTAGTAATTTTTATGCGAACATTATTTAGATTGTATTATCAAATTTAAAAGGGGG
GATAATTTTGGCAGGACATT

Product: NAD+ synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MYVDTHSKAEMMVESVSDWIKQQVEEAGCKGTVTGLSGGIDSAVVAALSKRVFPEDTLGVIMPCYSEPRDEEDALLFAEH
HNINVKNLDLSETYDSMVGKLDDTESTSKMALANIKPRLRMTVLYYYAQTYKYLVLGTSNKSELILGYFTKYGDGAADLL
PIATFTKMEVYALARQLEIPERLIKRPPTAGIISGQTDEDEMGFTYDELEKYFKGEQISKDVKNKIERLERINAHKRSMP
PIYTPKESLN

Sequences:

>Translated_250_residues
MYVDTHSKAEMMVESVSDWIKQQVEEAGCKGTVTGLSGGIDSAVVAALSKRVFPEDTLGVIMPCYSEPRDEEDALLFAEH
HNINVKNLDLSETYDSMVGKLDDTESTSKMALANIKPRLRMTVLYYYAQTYKYLVLGTSNKSELILGYFTKYGDGAADLL
PIATFTKMEVYALARQLEIPERLIKRPPTAGIISGQTDEDEMGFTYDELEKYFKGEQISKDVKNKIERLERINAHKRSMP
PIYTPKESLN
>Mature_250_residues
MYVDTHSKAEMMVESVSDWIKQQVEEAGCKGTVTGLSGGIDSAVVAALSKRVFPEDTLGVIMPCYSEPRDEEDALLFAEH
HNINVKNLDLSETYDSMVGKLDDTESTSKMALANIKPRLRMTVLYYYAQTYKYLVLGTSNKSELILGYFTKYGDGAADLL
PIATFTKMEVYALARQLEIPERLIKRPPTAGIISGQTDEDEMGFTYDELEKYFKGEQISKDVKNKIERLERINAHKRSMP
PIYTPKESLN

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=285, Percent_Identity=27.3684210526316, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1788036, Length=231, Percent_Identity=29.8701298701299, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538756, Length=257, Percent_Identity=30.3501945525292, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641841, Length=125, Percent_Identity=36, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641843, Length=125, Percent_Identity=36, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 28175; Mature: 28175

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVDTHSKAEMMVESVSDWIKQQVEEAGCKGTVTGLSGGIDSAVVAALSKRVFPEDTLGV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCE
IMPCYSEPRDEEDALLFAEHHNINVKNLDLSETYDSMVGKLDDTESTSKMALANIKPRLR
EECCCCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHH
MTVLYYYAQTYKYLVLGTSNKSELILGYFTKYGDGAADLLPIATFTKMEVYALARQLEIP
HHHHHHHHHHEEEEEEECCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
ERLIKRPPTAGIISGQTDEDEMGFTYDELEKYFKGEQISKDVKNKIERLERINAHKRSMP
HHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
PIYTPKESLN
CCCCCHHCCC
>Mature Secondary Structure
MYVDTHSKAEMMVESVSDWIKQQVEEAGCKGTVTGLSGGIDSAVVAALSKRVFPEDTLGV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCE
IMPCYSEPRDEEDALLFAEHHNINVKNLDLSETYDSMVGKLDDTESTSKMALANIKPRLR
EECCCCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHH
MTVLYYYAQTYKYLVLGTSNKSELILGYFTKYGDGAADLLPIATFTKMEVYALARQLEIP
HHHHHHHHHHEEEEEEECCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
ERLIKRPPTAGIISGQTDEDEMGFTYDELEKYFKGEQISKDVKNKIERLERINAHKRSMP
HHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
PIYTPKESLN
CCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA