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The map label for this gene is queA

Identifier: 188586408

GI number: 188586408

Start: 1857818

End: 1858849

Strand: Reverse

Name: queA

Synonym: Nther_1793

Alternate gene names: 188586408

Gene position: 1858849-1857818 (Counterclockwise)

Preceding gene: 188586409

Following gene: 188586407

Centisome position: 58.72

GC content: 37.5

Gene sequence:

>1032_bases
ATGAAATTAGAAGAATTTAATTATGAACTTCCAGAAGAATTAATCGCACAGAAACCACTTGCTAATAGATCTAAATCCAG
ATTAATGGTAGTTTCAATTAATTCATCAGATGTCCAGCACAACCATTTTGAAAAATTACCACAGTACGTAAAGGATGGAG
ATCTGTTTGTAATTAATAATAGTAGAGTAATACCTGCCAGATTATTTGGTAACAAAAAACAGACAGGTGGAAAACTGGAA
ATGGTGCTACTTCACCCCTTAGAGGGTAAAGATGAATGGGAAGTGCTGGTTAAACCGGGTAAGCGTGCTAAACCCGGGAA
TGTATTCGAATTTGGTCAAGAAACTTTGGAAGCAGAAATTTTAGATATCACTCCTGAAGGTAGTCGAAGAGTCAAATTTT
ATTACCAAGGAAATTTTCAACAGGTATTGGACCAATTGGGTCAAATGCCTTTGCCTCCATATATAAAGGAGGAATTAGAG
GATCCCGAACGATATCAGACTGTGTACGCCAAGGAAAGTGGGTCAGTAGCGGCTCCTACAGCTGGGTTACATTTCACACC
AGAAATCATAAGTGATATTAAGGATCAAGGTGGAGAGGTAGCCGAATTAACTTTACACGTAGGCCTAGGTACTTTTAGGC
CTGTAGAAACTCCTAATATTGAAGAACACGAAATGCATGCTGAATATTATCAACTCCCAGAAGAAACAGCCAAAAAAATT
AATCAAGCAAAACAAAGGGGTAATAGAGTGATTGCTGTAGGTACAACAGTAGTTCGTACTTTGGAGACAGTTAAAACTGA
CGATGGTTTAGTTCAGCCTGGAAAAGGTTGGACAGACATATTTATCTACCCCGGATACGAATTTAAAGTAGTTGACGCTA
TGCTAACTAATTTTCATTTGCCTAAATCTACATTGTTAATGTTAGTAAGTGCATTTGCTGGCAAAGATTTAATAATGAGC
GCTTATCAAGCTGCCATAGAGGAAAGATACCGGTTTTTCAGTTTTGGAGATGCCATGTTTATTAAAAATTAG

Upstream 100 bases:

>100_bases
GAATGGCCCTTGAAGGTTATAATTACAGGGAAATTTTAAACCACTATTACAGTGATCAAATAATAATTGAATAAAATAAA
ATAGTAAATGGGGTTGTAAT

Downstream 100 bases:

>100_bases
GTACTGGCACTGTTATAGTTAGTTGTTGATATTGTTACAGTTATCAACATTCAGCCAAAAATTAAATTGGCGATTTAGTT
ATTACAAGGAGCGCTGGAAA

Product: S-adenosylmethionine--tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKLEEFNYELPEELIAQKPLANRSKSRLMVVSINSSDVQHNHFEKLPQYVKDGDLFVINNSRVIPARLFGNKKQTGGKLE
MVLLHPLEGKDEWEVLVKPGKRAKPGNVFEFGQETLEAEILDITPEGSRRVKFYYQGNFQQVLDQLGQMPLPPYIKEELE
DPERYQTVYAKESGSVAAPTAGLHFTPEIISDIKDQGGEVAELTLHVGLGTFRPVETPNIEEHEMHAEYYQLPEETAKKI
NQAKQRGNRVIAVGTTVVRTLETVKTDDGLVQPGKGWTDIFIYPGYEFKVVDAMLTNFHLPKSTLLMLVSAFAGKDLIMS
AYQAAIEERYRFFSFGDAMFIKN

Sequences:

>Translated_343_residues
MKLEEFNYELPEELIAQKPLANRSKSRLMVVSINSSDVQHNHFEKLPQYVKDGDLFVINNSRVIPARLFGNKKQTGGKLE
MVLLHPLEGKDEWEVLVKPGKRAKPGNVFEFGQETLEAEILDITPEGSRRVKFYYQGNFQQVLDQLGQMPLPPYIKEELE
DPERYQTVYAKESGSVAAPTAGLHFTPEIISDIKDQGGEVAELTLHVGLGTFRPVETPNIEEHEMHAEYYQLPEETAKKI
NQAKQRGNRVIAVGTTVVRTLETVKTDDGLVQPGKGWTDIFIYPGYEFKVVDAMLTNFHLPKSTLLMLVSAFAGKDLIMS
AYQAAIEERYRFFSFGDAMFIKN
>Mature_343_residues
MKLEEFNYELPEELIAQKPLANRSKSRLMVVSINSSDVQHNHFEKLPQYVKDGDLFVINNSRVIPARLFGNKKQTGGKLE
MVLLHPLEGKDEWEVLVKPGKRAKPGNVFEFGQETLEAEILDITPEGSRRVKFYYQGNFQQVLDQLGQMPLPPYIKEELE
DPERYQTVYAKESGSVAAPTAGLHFTPEIISDIKDQGGEVAELTLHVGLGTFRPVETPNIEEHEMHAEYYQLPEETAKKI
NQAKQRGNRVIAVGTTVVRTLETVKTDDGLVQPGKGWTDIFIYPGYEFKVVDAMLTNFHLPKSTLLMLVSAFAGKDLIMS
AYQAAIEERYRFFSFGDAMFIKN

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=46.6666666666667, Blast_Score=308, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_NATTJ (B2A5L1)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917953.1
- ProteinModelPortal:   B2A5L1
- SMR:   B2A5L1
- GeneID:   6315256
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1793
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38858; Mature: 38858

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEEFNYELPEELIAQKPLANRSKSRLMVVSINSSDVQHNHFEKLPQYVKDGDLFVINN
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEC
SRVIPARLFGNKKQTGGKLEMVLLHPLEGKDEWEVLVKPGKRAKPGNVFEFGQETLEAEI
CEEEEHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCEEE
LDITPEGSRRVKFYYQGNFQQVLDQLGQMPLPPYIKEELEDPERYQTVYAKESGSVAAPT
EEECCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHEEEECCCCCEECCC
AGLHFTPEIISDIKDQGGEVAELTLHVGLGTFRPVETPNIEEHEMHAEYYQLPEETAKKI
CCCEECHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
NQAKQRGNRVIAVGTTVVRTLETVKTDDGLVQPGKGWTDIFIYPGYEFKVVDAMLTNFHL
HHHHHHCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHCCC
PKSTLLMLVSAFAGKDLIMSAYQAAIEERYRFFSFGDAMFIKN
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MKLEEFNYELPEELIAQKPLANRSKSRLMVVSINSSDVQHNHFEKLPQYVKDGDLFVINN
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEC
SRVIPARLFGNKKQTGGKLEMVLLHPLEGKDEWEVLVKPGKRAKPGNVFEFGQETLEAEI
CEEEEHHHCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCEEE
LDITPEGSRRVKFYYQGNFQQVLDQLGQMPLPPYIKEELEDPERYQTVYAKESGSVAAPT
EEECCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHEEEECCCCCEECCC
AGLHFTPEIISDIKDQGGEVAELTLHVGLGTFRPVETPNIEEHEMHAEYYQLPEETAKKI
CCCEECHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
NQAKQRGNRVIAVGTTVVRTLETVKTDDGLVQPGKGWTDIFIYPGYEFKVVDAMLTNFHL
HHHHHHCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHCCC
PKSTLLMLVSAFAGKDLIMSAYQAAIEERYRFFSFGDAMFIKN
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA