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The map label for this gene is ispG

Identifier: 188586063

GI number: 188586063

Start: 1503317

End: 1504387

Strand: Direct

Name: ispG

Synonym: Nther_1437

Alternate gene names: 188586063

Gene position: 1503317-1504387 (Clockwise)

Preceding gene: 188586062

Following gene: 188586064

Centisome position: 47.49

GC content: 40.15

Gene sequence:

>1071_bases
ATGTCTAGACGTCATAGAACTTTTCCTGTAAAAGTTGGATCTGTCACTATTGGAGGAACTGCTCCGGTTACCATCCAATC
AATGACCAATACAGATACCAAAGATACTGAAAAAACTTTAGCCCAAATCAAGGATTTGGTTGAAGCTGGTTGCCAATTAG
TTAGAGTGGCGATTCCTGATGAAGAATCTGTTAACAGTTTTAAAACTTTAACACAGTTTTCACCAGTCCCTTTGATTGCG
GACATCCACTTTAGTTATCAATTAGCAATCAAAGCAATAGAGGCAGGTGCCAGCAAAATTAGAATAAACCCCGGTAATAT
TGGGTCACGTCAAAGAGTGGCAAAGGTTGTCGAAAAAGCCAAGACTCACAATGTGCCAATTCGAGTGGGCATTAATTCAG
GATCAGTTGAAAAAAACCTCCTCCAAAAATATGGTGGACCTACTCCAAGTGCATTGGTGGAAAGCGCTGTCAATAATGTC
ATGATGTTATCTGAGATGGGTTTTGGCGATGTAGTAGTATCCATTAAAGCTTCGGATGTCAATACTACAGTTAAAGCTAA
CCAAGAGTTTGCCACAAGATTACCTAATCCTTTGCATTTAGGCATAACAGAGGCAGGGACTATTAAACAAGGTACTATTA
AGAGTTCTGTAGGAATTGGAACTTTACTATCTCATGGAATTGGAGATACACTGCGAGTTTCATTATCGGGTTCTCCTATA
GAAGAAGTTTCTGTTGCGCGCGGAATATTATCTTCTTTGAATTTAGCTGAAGGGCCTCGGATTGTATCATGCCCTACTTG
TGCCAGATCAAATATATCTGTCGAGGACTTGGCCAGTACCGTAGAAGACAGGCTAAAAGATCTCAACACTTCTCTCACTG
TTGCTGTTATGGGTTGTGAAGTCAATGGTCCAGGTGAAGCCAAGGAAGCTGATATAGGAATTGCTGGCAGTAAAGAATAT
GGGGTACTGTTCAAAAAGGGAAAGATTATAGATAGAGTACCTAAAAATCAATTACTTGAAGTCCTGTCTCGAGCCATCGA
TGAATATATTGATGAGATTCACAGCAAGTAA

Upstream 100 bases:

>100_bases
TTTGTTCATTTTATTGGGTTTGCTTTTTTAATTATGCTTATGTTTGTAATTTTGTATAATGATTTGATGAGATTGGAAAT
ATTTTAATAGGAGGTAAGTG

Downstream 100 bases:

>100_bases
TTATAGGGAAAACTGATCAAATAAATATGATTTCGGAGGTTTTGGCCGTGAATGAAACAGTAGCGATAATACCGGCATAT
AACGAAGAGAGTACTATCGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSRRHRTFPVKVGSVTIGGTAPVTIQSMTNTDTKDTEKTLAQIKDLVEAGCQLVRVAIPDEESVNSFKTLTQFSPVPLIA
DIHFSYQLAIKAIEAGASKIRINPGNIGSRQRVAKVVEKAKTHNVPIRVGINSGSVEKNLLQKYGGPTPSALVESAVNNV
MMLSEMGFGDVVVSIKASDVNTTVKANQEFATRLPNPLHLGITEAGTIKQGTIKSSVGIGTLLSHGIGDTLRVSLSGSPI
EEVSVARGILSSLNLAEGPRIVSCPTCARSNISVEDLASTVEDRLKDLNTSLTVAVMGCEVNGPGEAKEADIGIAGSKEY
GVLFKKGKIIDRVPKNQLLEVLSRAIDEYIDEIHSK

Sequences:

>Translated_356_residues
MSRRHRTFPVKVGSVTIGGTAPVTIQSMTNTDTKDTEKTLAQIKDLVEAGCQLVRVAIPDEESVNSFKTLTQFSPVPLIA
DIHFSYQLAIKAIEAGASKIRINPGNIGSRQRVAKVVEKAKTHNVPIRVGINSGSVEKNLLQKYGGPTPSALVESAVNNV
MMLSEMGFGDVVVSIKASDVNTTVKANQEFATRLPNPLHLGITEAGTIKQGTIKSSVGIGTLLSHGIGDTLRVSLSGSPI
EEVSVARGILSSLNLAEGPRIVSCPTCARSNISVEDLASTVEDRLKDLNTSLTVAVMGCEVNGPGEAKEADIGIAGSKEY
GVLFKKGKIIDRVPKNQLLEVLSRAIDEYIDEIHSK
>Mature_355_residues
SRRHRTFPVKVGSVTIGGTAPVTIQSMTNTDTKDTEKTLAQIKDLVEAGCQLVRVAIPDEESVNSFKTLTQFSPVPLIAD
IHFSYQLAIKAIEAGASKIRINPGNIGSRQRVAKVVEKAKTHNVPIRVGINSGSVEKNLLQKYGGPTPSALVESAVNNVM
MLSEMGFGDVVVSIKASDVNTTVKANQEFATRLPNPLHLGITEAGTIKQGTIKSSVGIGTLLSHGIGDTLRVSLSGSPIE
EVSVARGILSSLNLAEGPRIVSCPTCARSNISVEDLASTVEDRLKDLNTSLTVAVMGCEVNGPGEAKEADIGIAGSKEYG
VLFKKGKIIDRVPKNQLLEVLSRAIDEYIDEIHSK

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=354, Percent_Identity=44.9152542372881, Blast_Score=316, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_NATTJ (B2A390)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917608.1
- ProteinModelPortal:   B2A390
- GeneID:   6314860
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1437
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 37863; Mature: 37732

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRHRTFPVKVGSVTIGGTAPVTIQSMTNTDTKDTEKTLAQIKDLVEAGCQLVRVAIPD
CCCCCCCCEEEECCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
EESVNSFKTLTQFSPVPLIADIHFSYQLAIKAIEAGASKIRINPGNIGSRQRVAKVVEKA
CHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
KTHNVPIRVGINSGSVEKNLLQKYGGPTPSALVESAVNNVMMLSEMGFGDVVVSIKASDV
HHCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCCCEEEEEEEECCC
NTTVKANQEFATRLPNPLHLGITEAGTIKQGTIKSSVGIGTLLSHGIGDTLRVSLSGSPI
CCEEECCHHHHHHCCCCEEECCCCCCCCCCCCCHHCCCHHHHHHCCCCCEEEEEECCCCH
EEVSVARGILSSLNLAEGPRIVSCPTCARSNISVEDLASTVEDRLKDLNTSLTVAVMGCE
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEE
VNGPGEAKEADIGIAGSKEYGVLFKKGKIIDRVPKNQLLEVLSRAIDEYIDEIHSK
ECCCCCCCCCCEEECCCCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRRHRTFPVKVGSVTIGGTAPVTIQSMTNTDTKDTEKTLAQIKDLVEAGCQLVRVAIPD
CCCCCCCEEEECCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
EESVNSFKTLTQFSPVPLIADIHFSYQLAIKAIEAGASKIRINPGNIGSRQRVAKVVEKA
CHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
KTHNVPIRVGINSGSVEKNLLQKYGGPTPSALVESAVNNVMMLSEMGFGDVVVSIKASDV
HHCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCCCEEEEEEEECCC
NTTVKANQEFATRLPNPLHLGITEAGTIKQGTIKSSVGIGTLLSHGIGDTLRVSLSGSPI
CCEEECCHHHHHHCCCCEEECCCCCCCCCCCCCHHCCCHHHHHHCCCCCEEEEEECCCCH
EEVSVARGILSSLNLAEGPRIVSCPTCARSNISVEDLASTVEDRLKDLNTSLTVAVMGCE
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEE
VNGPGEAKEADIGIAGSKEYGVLFKKGKIIDRVPKNQLLEVLSRAIDEYIDEIHSK
ECCCCCCCCCCEEECCCCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA