Click here to switch to the map view.

The map label for this gene is trmFO

Identifier: 188586008

GI number: 188586008

Start: 1454087

End: 1455388

Strand: Direct

Name: trmFO

Synonym: Nther_1382

Alternate gene names: 188586008

Gene position: 1454087-1455388 (Clockwise)

Preceding gene: 188586007

Following gene: 188586009

Centisome position: 45.93

GC content: 37.86

Gene sequence:

>1302_bases
ATGAGATTAACAGTGATAGGTGGTGGACTAGCTGGTTCAGAAGCTGCTTATCAGGCTGCACAACAAGGGGTAACTGTTGA
CTTGTATGAAATGAGGCCGGTTAAGACCACGGCTGCACATAAAACAGGTGAACTGGCTGAATTAGTATGTAGTAATAGCT
TGCGAGCTAAATCCTTGGAAAATGCAGCTGGACTATTAAAAGAGGAGCTGCGAAAATTCGATAGTTTGATTATGAAAGTT
GCTGATCAGGTACAAGTTCCTGCCGGTGGAGCTTTGGCTGTTGATAGAGAAGCTTTTAGTCAGAAAGTGACTGAGTATAT
TGAAAACCATCCGAATATAAATTTAATCACAGAGGAAGTTACAGAAATAAATGAAAGTCAGCCAACTGTTATAGCTACAG
GACCTCTAACATCAACATCGTTAACTGAGCGAATTCAAAAGTTAACTGGTACAGATTTTCTGTACTTTTATGATGCAGCT
GCCCCAATCGTCACATATGAATCAATTAATAAAAATATTGCTTATTGGGCATCCAGGTATGGCAAAGGTACACCTGACTA
TCTGAACTGTCCCATGACTAAAGAAGAGTATGAAGACTTTTATCAAGAGTTAATAAATGCTGAAAAAGTACCTTTAAAAT
CTTTTGAAAAGGAAGTAGTCTTTGAAGGTTGTATGCCTGTTGAACAAATGGCAAACCGTGGTAAGGATACTTTGGTTTTT
GGTCCTTTAAAACCTGTAGGACTTGAAAATCCAAATACAGGAGAAATGCCCTATGCAGTAGTGCAACTGAGAAAAGATAA
TCGTGAAGGAACTCTATACAATCTAGTTGGTTTTCAAACTAGGCTAAAATGGCCTGAACAAAGAAGAGTGTTTCGTAAAA
TTCCCGGGCTAGAAGAAGCTGAATTTGTCAGGTATGGAGTTATGCATAGAAATACTTTTATTAATTCACCCAAAGTTTTA
ACCGCTAATTATCAGCTTAGAGAAAGGCCTGGATTATTTTTTGCAGGACAGGTGACTGGAGTTGAAGGATATGTAGAGTC
AACAGCTTCAGGGTTAGTTGCTGGACTTAATGCAGCTAGGAAATTACAAGGAAATGAAACCTTGACTTTCCCGCGAGAAA
CGGCATTGGGTGCCCTGGCAAGTTATATAGTTGAAGCTTCGCCTGACAATTTTCAACCTATGAACATTAATTTTGGTTTA
CTCCCCTCTTTAGAGAAGAAAGTTCCGAAAAAAATTAAAAAGCAAAAACAATCGGAAAGAGCTTTAAAAATATTGGAAGA
ATTTATTGAAAGAAAATTGTAA

Upstream 100 bases:

>100_bases
ACCAGTGTAGTCATCCAGATTGTGATTATTCCGCCGATGCCGTGGAAGAACAAGAAGAGAAACAGAGCTGAACTAACACA
GGAGAAGGGAAGGATATACT

Downstream 100 bases:

>100_bases
CTATTTTAGTTGCAAATCTTTTACGAATTGTGTTATGATTTGTCAGAAGGGAGAACTCGAAATTGAGAAACGAGTTGCTC
GACTTTTTGGAATTTTTAAA

Product: gid protein

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRLTVIGGGLAGSEAAYQAAQQGVTVDLYEMRPVKTTAAHKTGELAELVCSNSLRAKSLENAAGLLKEELRKFDSLIMKV
ADQVQVPAGGALAVDREAFSQKVTEYIENHPNINLITEEVTEINESQPTVIATGPLTSTSLTERIQKLTGTDFLYFYDAA
APIVTYESINKNIAYWASRYGKGTPDYLNCPMTKEEYEDFYQELINAEKVPLKSFEKEVVFEGCMPVEQMANRGKDTLVF
GPLKPVGLENPNTGEMPYAVVQLRKDNREGTLYNLVGFQTRLKWPEQRRVFRKIPGLEEAEFVRYGVMHRNTFINSPKVL
TANYQLRERPGLFFAGQVTGVEGYVESTASGLVAGLNAARKLQGNETLTFPRETALGALASYIVEASPDNFQPMNINFGL
LPSLEKKVPKKIKKQKQSERALKILEEFIERKL

Sequences:

>Translated_433_residues
MRLTVIGGGLAGSEAAYQAAQQGVTVDLYEMRPVKTTAAHKTGELAELVCSNSLRAKSLENAAGLLKEELRKFDSLIMKV
ADQVQVPAGGALAVDREAFSQKVTEYIENHPNINLITEEVTEINESQPTVIATGPLTSTSLTERIQKLTGTDFLYFYDAA
APIVTYESINKNIAYWASRYGKGTPDYLNCPMTKEEYEDFYQELINAEKVPLKSFEKEVVFEGCMPVEQMANRGKDTLVF
GPLKPVGLENPNTGEMPYAVVQLRKDNREGTLYNLVGFQTRLKWPEQRRVFRKIPGLEEAEFVRYGVMHRNTFINSPKVL
TANYQLRERPGLFFAGQVTGVEGYVESTASGLVAGLNAARKLQGNETLTFPRETALGALASYIVEASPDNFQPMNINFGL
LPSLEKKVPKKIKKQKQSERALKILEEFIERKL
>Mature_433_residues
MRLTVIGGGLAGSEAAYQAAQQGVTVDLYEMRPVKTTAAHKTGELAELVCSNSLRAKSLENAAGLLKEELRKFDSLIMKV
ADQVQVPAGGALAVDREAFSQKVTEYIENHPNINLITEEVTEINESQPTVIATGPLTSTSLTERIQKLTGTDFLYFYDAA
APIVTYESINKNIAYWASRYGKGTPDYLNCPMTKEEYEDFYQELINAEKVPLKSFEKEVVFEGCMPVEQMANRGKDTLVF
GPLKPVGLENPNTGEMPYAVVQLRKDNREGTLYNLVGFQTRLKWPEQRRVFRKIPGLEEAEFVRYGVMHRNTFINSPKVL
TANYQLRERPGLFFAGQVTGVEGYVESTASGLVAGLNAARKLQGNETLTFPRETALGALASYIVEASPDNFQPMNINFGL
LPSLEKKVPKKIKKQKQSERALKILEEFIERKL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=84, Percent_Identity=41.6666666666667, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_NATTJ (B2A334)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917553.1
- ProteinModelPortal:   B2A334
- SMR:   B2A334
- GeneID:   6316635
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1382
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 48281; Mature: 48281

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTVIGGGLAGSEAAYQAAQQGVTVDLYEMRPVKTTAAHKTGELAELVCSNSLRAKSLE
CEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCHHHCCCCHHHHHHHHCCCHHHHHHH
NAAGLLKEELRKFDSLIMKVADQVQVPAGGALAVDREAFSQKVTEYIENHPNINLITEEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHCCCCCEEEHHHH
TEINESQPTVIATGPLTSTSLTERIQKLTGTDFLYFYDAAAPIVTYESINKNIAYWASRY
HHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEHHHHCCHHHHHHHHC
GKGTPDYLNCPMTKEEYEDFYQELINAEKVPLKSFEKEVVFEGCMPVEQMANRGKDTLVF
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCEEEE
GPLKPVGLENPNTGEMPYAVVQLRKDNREGTLYNLVGFQTRLKWPEQRRVFRKIPGLEEA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEHHHHHHCCCCHHHHHHHHCCCCCHH
EFVRYGVMHRNTFINSPKVLTANYQLRERPGLFFAGQVTGVEGYVESTASGLVAGLNAAR
HHHHHHHHHHCCCCCCCCEEEECCEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
KLQGNETLTFPRETALGALASYIVEASPDNFQPMNINFGLLPSLEKKVPKKIKKQKQSER
HCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHH
ALKILEEFIERKL
HHHHHHHHHHHCC
>Mature Secondary Structure
MRLTVIGGGLAGSEAAYQAAQQGVTVDLYEMRPVKTTAAHKTGELAELVCSNSLRAKSLE
CEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCHHHCCCCHHHHHHHHCCCHHHHHHH
NAAGLLKEELRKFDSLIMKVADQVQVPAGGALAVDREAFSQKVTEYIENHPNINLITEEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHCCCCCEEEHHHH
TEINESQPTVIATGPLTSTSLTERIQKLTGTDFLYFYDAAAPIVTYESINKNIAYWASRY
HHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEHHHHCCHHHHHHHHC
GKGTPDYLNCPMTKEEYEDFYQELINAEKVPLKSFEKEVVFEGCMPVEQMANRGKDTLVF
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCEEEE
GPLKPVGLENPNTGEMPYAVVQLRKDNREGTLYNLVGFQTRLKWPEQRRVFRKIPGLEEA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEHHHHHHCCCCHHHHHHHHCCCCCHH
EFVRYGVMHRNTFINSPKVLTANYQLRERPGLFFAGQVTGVEGYVESTASGLVAGLNAAR
HHHHHHHHHHCCCCCCCCEEEECCEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
KLQGNETLTFPRETALGALASYIVEASPDNFQPMNINFGLLPSLEKKVPKKIKKQKQSER
HCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHH
ALKILEEFIERKL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA