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The map label for this gene is topA [H]

Identifier: 188586007

GI number: 188586007

Start: 1451970

End: 1454057

Strand: Direct

Name: topA [H]

Synonym: Nther_1381

Alternate gene names: 188586007

Gene position: 1451970-1454057 (Clockwise)

Preceding gene: 188586006

Following gene: 188586008

Centisome position: 45.87

GC content: 37.98

Gene sequence:

>2088_bases
TTGGCAAGTAATTTAGTTATAGTTGAATCTCCGGCCAAGGCTCAGACGATTAAAAGATACCTTGGAAAACAATACAAAGT
AAAAGCATCAATGGGGCATTTAATAGACCTCCCAAAAAGTCAGATGGGAATAGAAATTAATGAAGTATTCAAGCCAAAAT
ATATAACAATTAGAGGGAAAGGTAAAATTCTCTCAGAGCTTAAAAAAGAGGCCAAAAAAGCCGACAGAGTTTATCTGGCA
GGGGACCCTGACCGAGAAGGGGAAGCTATCTGTTGGCACTTGGGGCGAGCTTTAGGAATAGATGAAGATGAACATTGCCG
CGTCGAATTTAATGAAATTACTAAGGATGCAATAAAAGCGGCTTTTAAAGAACCAAGAAAAATCGCTTATAACTTAGTTA
ATGCACAACAGGCTCGACGAGTACTGGACCGGCTTGTTGGTTATAATATTAGTCCCATTTTGTGGAAAAAAGTAAGAAAA
GGATTGAGTGCGGGAAGGGTTCAGTCGGTAGCGGTTAGGCTCATTTGCGATAGAGAACAAGAGATCAAAGAATTTGTTCC
AGAAGAGTATTGGACCCTGATTGCTCAATTCAGTAAAGATGGAGAAAAATTTGAAGGTAAACTGCAAGAAGTTGACGGCA
AAAAAGCTGAGATTCCCAATGAAGAAAGAATGAACGAGATACTATCAAAGTTAGAAAATGCTACGTACAAGGTTCATAAG
GTAGAGAAAAAAGAACGCAAAAGAAACCCGAATCCTCCATTCATAACAAGCACATTACAGCAAGACGCATATAGACGATT
GGGTTTTTCTGCACGAAGAACTATGAGAACAGCTCAACAATTATATGAAGGTATTAAGGTGTCTGGTGAAGGACATGTGG
GTTTAATAACTTATTTAAGAACGGATTCCACTAGGATTAGTCAAAGTGCTTTGACTGCTGCTAAAGAATATATCCTGGAG
GAATATGGCGAAGACTACTATCCCGGGAAACCGAGAACGTTTAAAGGTGGCAAAGGATCCCAGGATGCTCACGAGTGTAT
TCGCCCAACTAGGACGGATTTATCACCTGAAAAAATTAAATCGGCCCTGAGTAATGACCAATTTAAATTGTATAAATTAA
TTTGGAATCGATTTATTGCAAGTCAGATGGCTCCAGCCAAGTACGATACCGTTGCTGCTGATATTTATGCTGCTGGTACT
ATGAAGTTTAGGGCTCGAGGTTCTCAACTTAAATTTCCAGGCTTTTTAGCTTTGGGACATGAAGACCAGGATAAAGATGA
AATTTTGCCTGAATTAAAACAGGATGATTTGTTAGATTTAGAAGAATTGCAACCTAACCAACACTTTACTCAACCACCGC
CTCGATACAGTGAGGCCTCTCTTGTAAAAACTTTAGAAAAAGAAGGTATAGGTCGTCCTAGTACTTATGCACCAATTATA
GAAACTATTCAAACAAAGGGGTATGTGATTCAGGAGGAGAAGAGACTATATCCGACTCAACTTGGTGAAATAGTAGTTGA
ACAACTTAAAGAATTTTTTCCTGATGTATTAGATGTAGATTTTACTGCAGAAGTAGAACAGGGTTTAGATAAAATTGAAG
ATGGTGAAAAAGACTGGCAAGAGTTCTTATATGAATTCTACGAACCCTTTGAGAAACAAGTAAAAACAGCTGAAGAAGAG
ATGAAGAAAATTGAACTAGAAGAAGAACAGACGGATGTAGAATGTGAAAAATGTGGTAGTTACATGGTGGTAAAACATGG
CCGTTATGGAAAGTTTTTGGCTTGTCCTAATTTTCCTGATTGTAAAAATACCAAATCCTTTGTTAAGGAATTGAATGTAG
AATGTCCTAAATGTCAGGGAACTATTGTAGAAAGAAAGAGTAAAAAAGGCAGAAAGTTTTTGGGATGCAATAATTATCCA
GACTGTGATTTTATAACTTGGTATAAACCTGTTGAAGGAAAAAAATGCCCTAAATGCGAATCTTTTCTAGTACAGCGCGG
TAAAAAGAACAAGTACTACCAGTGTAGTCATCCAGATTGTGATTATTCCGCCGATGCCGTGGAAGAACAAGAAGAGAAAC
AGAGCTGA

Upstream 100 bases:

>100_bases
ATACATGTTTATTGGAATTAGAATTAAATGGATGGATAATGCGCAGACTGGGTGGATATGTAATAAGGATAAAATAAGGT
CTTTTATGGAGGTGAAGTAA

Downstream 100 bases:

>100_bases
ACTAACACAGGAGAAGGGAAGGATATACTATGAGATTAACAGTGATAGGTGGTGGACTAGCTGGTTCAGAAGCTGCTTAT
CAGGCTGCACAACAAGGGGT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MASNLVIVESPAKAQTIKRYLGKQYKVKASMGHLIDLPKSQMGIEINEVFKPKYITIRGKGKILSELKKEAKKADRVYLA
GDPDREGEAICWHLGRALGIDEDEHCRVEFNEITKDAIKAAFKEPRKIAYNLVNAQQARRVLDRLVGYNISPILWKKVRK
GLSAGRVQSVAVRLICDREQEIKEFVPEEYWTLIAQFSKDGEKFEGKLQEVDGKKAEIPNEERMNEILSKLENATYKVHK
VEKKERKRNPNPPFITSTLQQDAYRRLGFSARRTMRTAQQLYEGIKVSGEGHVGLITYLRTDSTRISQSALTAAKEYILE
EYGEDYYPGKPRTFKGGKGSQDAHECIRPTRTDLSPEKIKSALSNDQFKLYKLIWNRFIASQMAPAKYDTVAADIYAAGT
MKFRARGSQLKFPGFLALGHEDQDKDEILPELKQDDLLDLEELQPNQHFTQPPPRYSEASLVKTLEKEGIGRPSTYAPII
ETIQTKGYVIQEEKRLYPTQLGEIVVEQLKEFFPDVLDVDFTAEVEQGLDKIEDGEKDWQEFLYEFYEPFEKQVKTAEEE
MKKIELEEEQTDVECEKCGSYMVVKHGRYGKFLACPNFPDCKNTKSFVKELNVECPKCQGTIVERKSKKGRKFLGCNNYP
DCDFITWYKPVEGKKCPKCESFLVQRGKKNKYYQCSHPDCDYSADAVEEQEEKQS

Sequences:

>Translated_695_residues
MASNLVIVESPAKAQTIKRYLGKQYKVKASMGHLIDLPKSQMGIEINEVFKPKYITIRGKGKILSELKKEAKKADRVYLA
GDPDREGEAICWHLGRALGIDEDEHCRVEFNEITKDAIKAAFKEPRKIAYNLVNAQQARRVLDRLVGYNISPILWKKVRK
GLSAGRVQSVAVRLICDREQEIKEFVPEEYWTLIAQFSKDGEKFEGKLQEVDGKKAEIPNEERMNEILSKLENATYKVHK
VEKKERKRNPNPPFITSTLQQDAYRRLGFSARRTMRTAQQLYEGIKVSGEGHVGLITYLRTDSTRISQSALTAAKEYILE
EYGEDYYPGKPRTFKGGKGSQDAHECIRPTRTDLSPEKIKSALSNDQFKLYKLIWNRFIASQMAPAKYDTVAADIYAAGT
MKFRARGSQLKFPGFLALGHEDQDKDEILPELKQDDLLDLEELQPNQHFTQPPPRYSEASLVKTLEKEGIGRPSTYAPII
ETIQTKGYVIQEEKRLYPTQLGEIVVEQLKEFFPDVLDVDFTAEVEQGLDKIEDGEKDWQEFLYEFYEPFEKQVKTAEEE
MKKIELEEEQTDVECEKCGSYMVVKHGRYGKFLACPNFPDCKNTKSFVKELNVECPKCQGTIVERKSKKGRKFLGCNNYP
DCDFITWYKPVEGKKCPKCESFLVQRGKKNKYYQCSHPDCDYSADAVEEQEEKQS
>Mature_694_residues
ASNLVIVESPAKAQTIKRYLGKQYKVKASMGHLIDLPKSQMGIEINEVFKPKYITIRGKGKILSELKKEAKKADRVYLAG
DPDREGEAICWHLGRALGIDEDEHCRVEFNEITKDAIKAAFKEPRKIAYNLVNAQQARRVLDRLVGYNISPILWKKVRKG
LSAGRVQSVAVRLICDREQEIKEFVPEEYWTLIAQFSKDGEKFEGKLQEVDGKKAEIPNEERMNEILSKLENATYKVHKV
EKKERKRNPNPPFITSTLQQDAYRRLGFSARRTMRTAQQLYEGIKVSGEGHVGLITYLRTDSTRISQSALTAAKEYILEE
YGEDYYPGKPRTFKGGKGSQDAHECIRPTRTDLSPEKIKSALSNDQFKLYKLIWNRFIASQMAPAKYDTVAADIYAAGTM
KFRARGSQLKFPGFLALGHEDQDKDEILPELKQDDLLDLEELQPNQHFTQPPPRYSEASLVKTLEKEGIGRPSTYAPIIE
TIQTKGYVIQEEKRLYPTQLGEIVVEQLKEFFPDVLDVDFTAEVEQGLDKIEDGEKDWQEFLYEFYEPFEKQVKTAEEEM
KKIELEEEQTDVECEKCGSYMVVKHGRYGKFLACPNFPDCKNTKSFVKELNVECPKCQGTIVERKSKKGRKFLGCNNYPD
CDFITWYKPVEGKKCPKCESFLVQRGKKNKYYQCSHPDCDYSADAVEEQEEKQS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=630, Percent_Identity=26.031746031746, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI4507635, Length=661, Percent_Identity=23.6006051437216, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI1787529, Length=750, Percent_Identity=41.6, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1788061, Length=529, Percent_Identity=23.4404536862004, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI48994931, Length=93, Percent_Identity=38.7096774193548, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32563869, Length=714, Percent_Identity=25.7703081232493, Blast_Score=142, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=511, Percent_Identity=26.027397260274, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=588, Percent_Identity=23.9795918367347, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585251, Length=631, Percent_Identity=23.2963549920761, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24640096, Length=547, Percent_Identity=24.4972577696526, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79865; Mature: 79734

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNLVIVESPAKAQTIKRYLGKQYKVKASMGHLIDLPKSQMGIEINEVFKPKYITIRGK
CCCCEEEEECCHHHHHHHHHHCCCEEEEHHHCHHHCCCCHHHCCCHHHCCCCCEEEEECC
GKILSELKKEAKKADRVYLAGDPDREGEAICWHLGRALGIDEDEHCRVEFNEITKDAIKA
CHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHH
AFKEPRKIAYNLVNAQQARRVLDRLVGYNISPILWKKVRKGLSAGRVQSVAVRLICDREQ
HHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHH
EIKEFVPEEYWTLIAQFSKDGEKFEGKLQEVDGKKAEIPNEERMNEILSKLENATYKVHK
HHHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHH
VEKKERKRNPNPPFITSTLQQDAYRRLGFSARRTMRTAQQLYEGIKVSGEGHVGLITYLR
HHHHHHCCCCCCCEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCEECCCCCEEEEEEEE
TDSTRISQSALTAAKEYILEEYGEDYYPGKPRTFKGGKGSQDAHECIRPTRTDLSPEKIK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHH
SALSNDQFKLYKLIWNRFIASQMAPAKYDTVAADIYAAGTMKFRARGSQLKFPGFLALGH
HHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECC
EDQDKDEILPELKQDDLLDLEELQPNQHFTQPPPRYSEASLVKTLEKEGIGRPSTYAPII
CCCCHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
ETIQTKGYVIQEEKRLYPTQLGEIVVEQLKEFFPDVLDVDFTAEVEQGLDKIEDGEKDWQ
HHHHCCCEEEEHHHHCCHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHCCCHHHHH
EFLYEFYEPFEKQVKTAEEEMKKIELEEEQTDVECEKCGSYMVVKHGRYGKFLACPNFPD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHCCCEEEEECCCCCCEEECCCCCC
CKNTKSFVKELNVECPKCQGTIVERKSKKGRKFLGCNNYPDCDFITWYKPVEGKKCPKCE
CCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCCCCCCHH
SFLVQRGKKNKYYQCSHPDCDYSADAVEEQEEKQS
HHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ASNLVIVESPAKAQTIKRYLGKQYKVKASMGHLIDLPKSQMGIEINEVFKPKYITIRGK
CCCEEEEECCHHHHHHHHHHCCCEEEEHHHCHHHCCCCHHHCCCHHHCCCCCEEEEECC
GKILSELKKEAKKADRVYLAGDPDREGEAICWHLGRALGIDEDEHCRVEFNEITKDAIKA
CHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHH
AFKEPRKIAYNLVNAQQARRVLDRLVGYNISPILWKKVRKGLSAGRVQSVAVRLICDREQ
HHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHH
EIKEFVPEEYWTLIAQFSKDGEKFEGKLQEVDGKKAEIPNEERMNEILSKLENATYKVHK
HHHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHHHH
VEKKERKRNPNPPFITSTLQQDAYRRLGFSARRTMRTAQQLYEGIKVSGEGHVGLITYLR
HHHHHHCCCCCCCEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCEECCCCCEEEEEEEE
TDSTRISQSALTAAKEYILEEYGEDYYPGKPRTFKGGKGSQDAHECIRPTRTDLSPEKIK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHH
SALSNDQFKLYKLIWNRFIASQMAPAKYDTVAADIYAAGTMKFRARGSQLKFPGFLALGH
HHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECC
EDQDKDEILPELKQDDLLDLEELQPNQHFTQPPPRYSEASLVKTLEKEGIGRPSTYAPII
CCCCHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
ETIQTKGYVIQEEKRLYPTQLGEIVVEQLKEFFPDVLDVDFTAEVEQGLDKIEDGEKDWQ
HHHHCCCEEEEHHHHCCHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHCCCHHHHH
EFLYEFYEPFEKQVKTAEEEMKKIELEEEQTDVECEKCGSYMVVKHGRYGKFLACPNFPD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHCCCEEEEECCCCCCEEECCCCCC
CKNTKSFVKELNVECPKCQGTIVERKSKKGRKFLGCNNYPDCDFITWYKPVEGKKCPKCE
CCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCCCCCCCHH
SFLVQRGKKNKYYQCSHPDCDYSADAVEEQEEKQS
HHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]