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The map label for this gene is trmD

Identifier: 188585998

GI number: 188585998

Start: 1443185

End: 1443955

Strand: Direct

Name: trmD

Synonym: Nther_1372

Alternate gene names: 188585998

Gene position: 1443185-1443955 (Clockwise)

Preceding gene: 188585997

Following gene: 188585999

Centisome position: 45.59

GC content: 37.87

Gene sequence:

>771_bases
ATGCATATCGATATCATGACATTATTCCCGGAAATGTTTCACGGCGTATTGGAAAGCAGTATTATTGGTAGAGCCCTGGC
TCGAGAAATGATTACAGTAAATCTGATTGATATCAGGGAGTTTTCTACTAATAAGCATCGAAAGGTAGATGATTACCCTT
ATGGTGGCGGTGCGGGAATGGTGATGAAACCAGAACCCATATTTTATGCCATGGATGAACTTAAAAAGTCTCCTTCTTTT
AATGTAAACCAGAAAACTGTTTTTGTTACACCACAAGGAAATCCATATTCTCAAGAAAAAGCGAAGGAATTATCTAGTTT
AAATCGATTAACCATTTTATGTGGGCATTACGAAGGAGTAGATGAAAGGGTTAGGCAACATTTAGTAGATGAAGAAATAT
CCGTTGGAGATTACGTATTAACCGGTGGTGAATTGCCTGCAATGGTTATTTTGGATTCTGTAACCAGGCTTGTACCTGGT
GTTCTTGGTGATGAGAGCTCGGCCAAGACAGATTCATTCAATAATTACTTGCTTGAACATCCCCAGTATACTAGACCTGC
AATTTTTAGAGGTTTAGAAGTTCCAGAAATTTTAATGTCTGGGGATCACAAGCGAATAGCAACATGGAAAACCAAAGAGA
GCCTAAAGAGAACCTTATCTCGGAGGCCAGACTTACTAGACAAGAAATCGTTAACTGAAGAAGAATACAAATTAATGCGC
GAAATTTTAAAAGAAAGTCCAAATAAAGAATTGGACTTGGACAGATTGTAA

Upstream 100 bases:

>100_bases
TTAATTCCAGCCCTTTATGAAGTAGTTAAAAAAATAGATCTTGACGAACAAATTATGATAGTGGATCTACCAGAGGGGCT
CTTAGACGAGGAGGAATAAA

Downstream 100 bases:

>100_bases
ATTTAAGACCAATATGCTATAATATTTCTGTCGTTTGTCAAATCACAGATGGTCCTCTGTCAAAATAATTGACATGAACG
TCTAAGAAGGAGGAGGAAGA

Product: tRNA (Guanine37-N(1)-) methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MHIDIMTLFPEMFHGVLESSIIGRALAREMITVNLIDIREFSTNKHRKVDDYPYGGGAGMVMKPEPIFYAMDELKKSPSF
NVNQKTVFVTPQGNPYSQEKAKELSSLNRLTILCGHYEGVDERVRQHLVDEEISVGDYVLTGGELPAMVILDSVTRLVPG
VLGDESSAKTDSFNNYLLEHPQYTRPAIFRGLEVPEILMSGDHKRIATWKTKESLKRTLSRRPDLLDKKSLTEEEYKLMR
EILKESPNKELDLDRL

Sequences:

>Translated_256_residues
MHIDIMTLFPEMFHGVLESSIIGRALAREMITVNLIDIREFSTNKHRKVDDYPYGGGAGMVMKPEPIFYAMDELKKSPSF
NVNQKTVFVTPQGNPYSQEKAKELSSLNRLTILCGHYEGVDERVRQHLVDEEISVGDYVLTGGELPAMVILDSVTRLVPG
VLGDESSAKTDSFNNYLLEHPQYTRPAIFRGLEVPEILMSGDHKRIATWKTKESLKRTLSRRPDLLDKKSLTEEEYKLMR
EILKESPNKELDLDRL
>Mature_256_residues
MHIDIMTLFPEMFHGVLESSIIGRALAREMITVNLIDIREFSTNKHRKVDDYPYGGGAGMVMKPEPIFYAMDELKKSPSF
NVNQKTVFVTPQGNPYSQEKAKELSSLNRLTILCGHYEGVDERVRQHLVDEEISVGDYVLTGGELPAMVILDSVTRLVPG
VLGDESSAKTDSFNNYLLEHPQYTRPAIFRGLEVPEILMSGDHKRIATWKTKESLKRTLSRRPDLLDKKSLTEEEYKLMR
EILKESPNKELDLDRL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=250, Percent_Identity=45.2, Blast_Score=234, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_NATTJ (B2A2P2)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917543.1
- ProteinModelPortal:   B2A2P2
- SMR:   B2A2P2
- GeneID:   6315685
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1372
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 29199; Mature: 29199

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIDIMTLFPEMFHGVLESSIIGRALAREMITVNLIDIREFSTNKHRKVDDYPYGGGAGM
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCCCCCCCCCCCCCCC
VMKPEPIFYAMDELKKSPSFNVNQKTVFVTPQGNPYSQEKAKELSSLNRLTILCGHYEGV
EECCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCH
DERVRQHLVDEEISVGDYVLTGGELPAMVILDSVTRLVPGVLGDESSAKTDSFNNYLLEH
HHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
PQYTRPAIFRGLEVPEILMSGDHKRIATWKTKESLKRTLSRRPDLLDKKSLTEEEYKLMR
CCCCCHHHHHCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHH
EILKESPNKELDLDRL
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MHIDIMTLFPEMFHGVLESSIIGRALAREMITVNLIDIREFSTNKHRKVDDYPYGGGAGM
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCCCCCCCCCCCCCCC
VMKPEPIFYAMDELKKSPSFNVNQKTVFVTPQGNPYSQEKAKELSSLNRLTILCGHYEGV
EECCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCH
DERVRQHLVDEEISVGDYVLTGGELPAMVILDSVTRLVPGVLGDESSAKTDSFNNYLLEH
HHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
PQYTRPAIFRGLEVPEILMSGDHKRIATWKTKESLKRTLSRRPDLLDKKSLTEEEYKLMR
CCCCCHHHHHCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHH
EILKESPNKELDLDRL
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA