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The map label for this gene is sigE [H]

Identifier: 188585936

GI number: 188585936

Start: 1384097

End: 1384816

Strand: Direct

Name: sigE [H]

Synonym: Nther_1310

Alternate gene names: 188585936

Gene position: 1384097-1384816 (Clockwise)

Preceding gene: 188585935

Following gene: 188585937

Centisome position: 43.72

GC content: 28.47

Gene sequence:

>720_bases
ATGAAATTTTTTAGAAATTTATTTGTTAAAATTGTTATAAAATTATACCAATGGAATATTATAAAAAAACCGCCAGTGCA
TTATATAGGGAGTAGTGAAGCCTTACCACCACCGTTAACAAATGAAGAAGAAAAAGATTTACTTGAAAAACTAAAAGCTG
GTGATAAAAAAGTAAGGTCAACTTTAATTGAGAGAAATTTAAGGCTGGTGGTTTATATTGCCAGAAAATTTGAAAATACT
GGTGTAAATATAGAAGATTTGATCTCTATAGGGACTATCGGCTTAATAAAAGCTGTTAATACTTTTGATCCTCAAAAGAA
AATTAAACTAGCTACCTATGCTTCAAAATGTATAGAAAATGAAATTTTGATGTATTTGAGACGCAATAATAAATTAAAAA
ATGAAGTATCAATCGATGAACCATTAAATATCGATTGGGACGGAAATGAACTCTTATTGTCTGATGTATTGGGAACTGAT
ATTGACATTATACATCAAAATATTGAGCAAAAAGTAAACAAAAATTTACTGAAGATGGCTTTGGAAAAATTATCCCAGAG
AGAAAAAACTATTATGAAATTGCGGTTTGGATTAGGGCGTCAAGAAGGTAAAACACAAAAAGAAGTAGCCGATATACTGG
GAATTTCTCAGTCTTATATTTCAAGATTAGAAAAGAGAATTATTAATAGAATGAGAAAAGAAATAAACAAAATGCAATAA

Upstream 100 bases:

>100_bases
TTTCTGCTCTAATAGCTGTTACTTTTCAAGAATTAAATATTGATGATACTAATTGTGGTTTAGTACCAGTAGAATTATTA
AAATTTGCGGAGGGATCTTG

Downstream 100 bases:

>100_bases
CTCACCAGTATAAAAAACGGGTACTTGGAGATAAATAAGGGACAGAATGAAGCAACAGGGGGCGATTAAAATGAGCAGTG
CTAAGGTAGAAATTTCCAAT

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: P31; Sigma-29; Stage II sporulation protein GB [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKFFRNLFVKIVIKLYQWNIIKKPPVHYIGSSEALPPPLTNEEEKDLLEKLKAGDKKVRSTLIERNLRLVVYIARKFENT
GVNIEDLISIGTIGLIKAVNTFDPQKKIKLATYASKCIENEILMYLRRNNKLKNEVSIDEPLNIDWDGNELLLSDVLGTD
IDIIHQNIEQKVNKNLLKMALEKLSQREKTIMKLRFGLGRQEGKTQKEVADILGISQSYISRLEKRIINRMRKEINKMQ

Sequences:

>Translated_239_residues
MKFFRNLFVKIVIKLYQWNIIKKPPVHYIGSSEALPPPLTNEEEKDLLEKLKAGDKKVRSTLIERNLRLVVYIARKFENT
GVNIEDLISIGTIGLIKAVNTFDPQKKIKLATYASKCIENEILMYLRRNNKLKNEVSIDEPLNIDWDGNELLLSDVLGTD
IDIIHQNIEQKVNKNLLKMALEKLSQREKTIMKLRFGLGRQEGKTQKEVADILGISQSYISRLEKRIINRMRKEINKMQ
>Mature_239_residues
MKFFRNLFVKIVIKLYQWNIIKKPPVHYIGSSEALPPPLTNEEEKDLLEKLKAGDKKVRSTLIERNLRLVVYIARKFENT
GVNIEDLISIGTIGLIKAVNTFDPQKKIKLATYASKCIENEILMYLRRNNKLKNEVSIDEPLNIDWDGNELLLSDVLGTD
IDIIHQNIEQKVNKNLLKMALEKLSQREKTIMKLRFGLGRQEGKTQKEVADILGISQSYISRLEKRIINRMRKEINKMQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=260, Percent_Identity=27.3076923076923, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789871, Length=106, Percent_Identity=41.5094339622642, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789448, Length=87, Percent_Identity=41.3793103448276, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27760; Mature: 27760

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFRNLFVKIVIKLYQWNIIKKPPVHYIGSSEALPPPLTNEEEKDLLEKLKAGDKKVRS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
TLIERNLRLVVYIARKFENTGVNIEDLISIGTIGLIKAVNTFDPQKKIKLATYASKCIEN
HHHHCCCHHEEHEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EILMYLRRNNKLKNEVSIDEPLNIDWDGNELLLSDVLGTDIDIIHQNIEQKVNKNLLKMA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LEKLSQREKTIMKLRFGLGRQEGKTQKEVADILGISQSYISRLEKRIINRMRKEINKMQ
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFFRNLFVKIVIKLYQWNIIKKPPVHYIGSSEALPPPLTNEEEKDLLEKLKAGDKKVRS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
TLIERNLRLVVYIARKFENTGVNIEDLISIGTIGLIKAVNTFDPQKKIKLATYASKCIEN
HHHHCCCHHEEHEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EILMYLRRNNKLKNEVSIDEPLNIDWDGNELLLSDVLGTDIDIIHQNIEQKVNKNLLKMA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LEKLSQREKTIMKLRFGLGRQEGKTQKEVADILGISQSYISRLEKRIINRMRKEINKMQ
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6438529; 9384377; 3104904; 2106671 [H]