Click here to switch to the map view.

The map label for this gene is sigG [H]

Identifier: 188585937

GI number: 188585937

Start: 1384887

End: 1385696

Strand: Direct

Name: sigG [H]

Synonym: Nther_1311

Alternate gene names: 188585937

Gene position: 1384887-1385696 (Clockwise)

Preceding gene: 188585936

Following gene: 188585938

Centisome position: 43.75

GC content: 36.42

Gene sequence:

>810_bases
ATGAGCAGTGCTAAGGTAGAAATTTCCAATGTCAACACCTCCAAGTTACCCGTTTTAAACAACTCAGAAATGAAGGAACT
CTTTGAGAAAATGAGGGTTGGTGATGATGAAGACGAAGCAAGACAAAAACTTATTAACGGGAATTTACGCCTTGTATTAA
GTGTGATCCAAAGGTTCAACAACAGAGGTGAATACGTAGATGATCTATTCCAAGTAGGTTGTGTAGGGTTAATAAAGGCT
ATTGATAATTTTGATTTGAGTCAAAACGTAAAATTTTCAACTTATGCAGTACCTATGATAATTGGTGAAATACGAAGATA
CTTAAGAGATAATAATTCTATTAGAGTTAGCCGCTCCCTAAGAGATATAGCATATAAAGCCCTTCAAATCAGAGATGAGC
TAGTTGAAAAAAATTCCAGAGAACCTGCAATATCTGAAATTGCCGAAAAACTCGATGTTAGTGAAGAACAGGTGGTATTT
GCTTTAGATGCTATCCAAGATCCAGTATCTTTATTTGAACCAATCTATCATGATGGGGGTGATCCCATCTTCGTAATGGA
TCAGATCAGTGATGAGAAGAATCAAGATAGAACCTGGTTAGAGGAAATAGCCATTAAAGAAGGTCTTCATAGACTATCCT
CCCGAGAAAAATTAATTGTAAATCTGCGATTTTTTAAGGGGAAAACACAAATGGAAGTAGCAGAAGAAATCGGAATCAGC
CAGGCCCAGGTTTCACGGCTAGAAAAAGCCGCTTTATCTCAATTGAAAAAAAATATATCCGATTCTGGATCAAAATCTAG
GTCGAATTAA

Upstream 100 bases:

>100_bases
ATGAGAAAAGAAATAAACAAAATGCAATAACTCACCAGTATAAAAAACGGGTACTTGGAGATAAATAAGGGACAGAATGA
AGCAACAGGGGGCGATTAAA

Downstream 100 bases:

>100_bases
ATTCCCTCCTCCAGGAGGGAATTTTTATTATAATAATTTAGGAAAAAAACCTTTCTGCTCATCATATGATGAATCAGGGG
GTATTAATGTGATTAAGGCT

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: Stage III sporulation protein G [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSSAKVEISNVNTSKLPVLNNSEMKELFEKMRVGDDEDEARQKLINGNLRLVLSVIQRFNNRGEYVDDLFQVGCVGLIKA
IDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSLRDIAYKALQIRDELVEKNSREPAISEIAEKLDVSEEQVVF
ALDAIQDPVSLFEPIYHDGGDPIFVMDQISDEKNQDRTWLEEIAIKEGLHRLSSREKLIVNLRFFKGKTQMEVAEEIGIS
QAQVSRLEKAALSQLKKNISDSGSKSRSN

Sequences:

>Translated_269_residues
MSSAKVEISNVNTSKLPVLNNSEMKELFEKMRVGDDEDEARQKLINGNLRLVLSVIQRFNNRGEYVDDLFQVGCVGLIKA
IDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSLRDIAYKALQIRDELVEKNSREPAISEIAEKLDVSEEQVVF
ALDAIQDPVSLFEPIYHDGGDPIFVMDQISDEKNQDRTWLEEIAIKEGLHRLSSREKLIVNLRFFKGKTQMEVAEEIGIS
QAQVSRLEKAALSQLKKNISDSGSKSRSN
>Mature_268_residues
SSAKVEISNVNTSKLPVLNNSEMKELFEKMRVGDDEDEARQKLINGNLRLVLSVIQRFNNRGEYVDDLFQVGCVGLIKAI
DNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSLRDIAYKALQIRDELVEKNSREPAISEIAEKLDVSEEQVVFA
LDAIQDPVSLFEPIYHDGGDPIFVMDQISDEKNQDRTWLEEIAIKEGLHRLSSREKLIVNLRFFKGKTQMEVAEEIGISQ
AQVSRLEKAALSQLKKNISDSGSKSRSN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the forespore [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=250, Percent_Identity=28.8, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789098, Length=235, Percent_Identity=29.3617021276596, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789871, Length=260, Percent_Identity=26.1538461538462, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788231, Length=200, Percent_Identity=26, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30595; Mature: 30464

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAKVEISNVNTSKLPVLNNSEMKELFEKMRVGDDEDEARQKLINGNLRLVLSVIQRFN
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
NRGEYVDDLFQVGCVGLIKAIDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHH
RDIAYKALQIRDELVEKNSREPAISEIAEKLDVSEEQVVFALDAIQDPVSLFEPIYHDGG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCC
DPIFVMDQISDEKNQDRTWLEEIAIKEGLHRLSSREKLIVNLRFFKGKTQMEVAEEIGIS
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCC
QAQVSRLEKAALSQLKKNISDSGSKSRSN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSAKVEISNVNTSKLPVLNNSEMKELFEKMRVGDDEDEARQKLINGNLRLVLSVIQRFN
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
NRGEYVDDLFQVGCVGLIKAIDNFDLSQNVKFSTYAVPMIIGEIRRYLRDNNSIRVSRSL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHH
RDIAYKALQIRDELVEKNSREPAISEIAEKLDVSEEQVVFALDAIQDPVSLFEPIYHDGG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCC
DPIFVMDQISDEKNQDRTWLEEIAIKEGLHRLSSREKLIVNLRFFKGKTQMEVAEEIGIS
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCC
QAQVSRLEKAALSQLKKNISDSGSKSRSN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497052; 2459711; 9384377 [H]