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The map label for this gene is murD

Identifier: 188585928

GI number: 188585928

Start: 1374723

End: 1376108

Strand: Direct

Name: murD

Synonym: Nther_1302

Alternate gene names: 188585928

Gene position: 1374723-1376108 (Clockwise)

Preceding gene: 188585927

Following gene: 188585929

Centisome position: 43.43

GC content: 34.7

Gene sequence:

>1386_bases
ATGTTTGATGATAAAGGCAAATATCTAATACTTGGATTAGGTAAAAGCGGCCAGGGCGCTGTTATAGGTCTGAGTAATTT
TGAAGATTGTCAGGTAGCTGTGACAGATAGAAAAAACCTCGATGACATCCGGGATGCAGCTGCCATACTAGAAGAGCATA
ATGTACCTTATTTAAATGAAAGAGAAAGCGTTGAAAGACTGTCTGAGTTTGATATTCTTGTCAAAAGTCCAGGAATACCC
ATGGACAATAATATTATTACCAAAGCTAAAGAATTAGGTATGCCTGTCATAGATGAACTTGAACTAGGATATCAAATATT
GAATTACAGATTGACAAAACCGGAAGAACGGCTTATTGCTGTAACTGGTACTAATGGAAAAACTACAACTACTAATTTAA
TTGGCGAATTTTTCGAAGCAAGTGGTTACAGCATTTATGTAGCGGGAAATGTGGGATTACCTTTAAGCCAGATAGCCTGT
GAAGTTACAGACACTGATTTTATAGTCTTAGAAGTATCTAGTTTTCAATTGGAATTTATTAAAGGCTTTAGGCCAAAGGT
ATCAATGATATTGAATATAACACCAGATCACCTAGATTGGCATGGTGATCTAGATTCATATATTAGAGCTAAGATGAATA
TTTTTAAAAATCAGACCAGTAAAGATTTTTCCGTTGTCAATGTGGATGATGAAACGACATTTGGTTTAAATCGAGAAAGC
AAAGGAAGAAAATTAAATTTTTCAAAGAATAGTTTGCGGACTGAAGGCTCTTTTATAGAGGATGATCACTTAGTAATCAA
TTATTTAGATACTAAAACTTCTATAATCCATAAACACGATATTTTACTTCCTGGGGAGCATAATTTGGAGAATGTTTTGG
CAGCGACTACCGCAACTTTGCCTTTTTCTATAAGTGAGGAAAATATTCGAAATACCTTGAAAGCATTTAGTGGAGTAGAC
CACAGACTTGAATTAGTGAGAGAACTAAATGGAGTTAAATTTATAAATGATTCTAAGGGAACCAATGTTGAAGCCTCTTT
AAAAGCCATCAATTCATTTGATTCCATGATATTAATAGCTGGGGGTAAAGATAAAGGGGCTGATTTCAGTGAATTTGCGG
AGGCTATAAAAGAAAAAGGAGTTAAAAAAGTTTATTTATTTGGAGAAACTTATAAAAAAATAGCTTCTGCTCTAGATAAA
GTATATTATCCTGAATATTATATTGTTAATAATTTAGAAACAGCTGTCACAGGGGCATATCAGGACGCCATTTCTGGCGA
TGTAGTTTTGCTTTCACCTGCCTGTGCCAGCTGGGATATGTATGACAGTTTCGAGAAACGGGGAAATCATTTTAAAAGTA
TTGTTTATTCTCTCGGGGGTGAATGA

Upstream 100 bases:

>100_bases
AGGTTGTATTAGTTTTTTGGGTTTTTACTCTGATTACTGCCGCTATCGGGATTATTTCCGTGATTAATTTATAAACAAAA
GTTCAGATAGGAGAGAGACT

Downstream 100 bases:

>100_bases
TATGGAAAAAAAGCAATCAACTCCCGATTTCACTCTTTTTGCAGTTACACTAATATTGGTAGCTTTTGGATTGGTAATGG
TTTTTAGTTCTAGTGCTATC

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MFDDKGKYLILGLGKSGQGAVIGLSNFEDCQVAVTDRKNLDDIRDAAAILEEHNVPYLNERESVERLSEFDILVKSPGIP
MDNNIITKAKELGMPVIDELELGYQILNYRLTKPEERLIAVTGTNGKTTTTNLIGEFFEASGYSIYVAGNVGLPLSQIAC
EVTDTDFIVLEVSSFQLEFIKGFRPKVSMILNITPDHLDWHGDLDSYIRAKMNIFKNQTSKDFSVVNVDDETTFGLNRES
KGRKLNFSKNSLRTEGSFIEDDHLVINYLDTKTSIIHKHDILLPGEHNLENVLAATTATLPFSISEENIRNTLKAFSGVD
HRLELVRELNGVKFINDSKGTNVEASLKAINSFDSMILIAGGKDKGADFSEFAEAIKEKGVKKVYLFGETYKKIASALDK
VYYPEYYIVNNLETAVTGAYQDAISGDVVLLSPACASWDMYDSFEKRGNHFKSIVYSLGGE

Sequences:

>Translated_461_residues
MFDDKGKYLILGLGKSGQGAVIGLSNFEDCQVAVTDRKNLDDIRDAAAILEEHNVPYLNERESVERLSEFDILVKSPGIP
MDNNIITKAKELGMPVIDELELGYQILNYRLTKPEERLIAVTGTNGKTTTTNLIGEFFEASGYSIYVAGNVGLPLSQIAC
EVTDTDFIVLEVSSFQLEFIKGFRPKVSMILNITPDHLDWHGDLDSYIRAKMNIFKNQTSKDFSVVNVDDETTFGLNRES
KGRKLNFSKNSLRTEGSFIEDDHLVINYLDTKTSIIHKHDILLPGEHNLENVLAATTATLPFSISEENIRNTLKAFSGVD
HRLELVRELNGVKFINDSKGTNVEASLKAINSFDSMILIAGGKDKGADFSEFAEAIKEKGVKKVYLFGETYKKIASALDK
VYYPEYYIVNNLETAVTGAYQDAISGDVVLLSPACASWDMYDSFEKRGNHFKSIVYSLGGE
>Mature_461_residues
MFDDKGKYLILGLGKSGQGAVIGLSNFEDCQVAVTDRKNLDDIRDAAAILEEHNVPYLNERESVERLSEFDILVKSPGIP
MDNNIITKAKELGMPVIDELELGYQILNYRLTKPEERLIAVTGTNGKTTTTNLIGEFFEASGYSIYVAGNVGLPLSQIAC
EVTDTDFIVLEVSSFQLEFIKGFRPKVSMILNITPDHLDWHGDLDSYIRAKMNIFKNQTSKDFSVVNVDDETTFGLNRES
KGRKLNFSKNSLRTEGSFIEDDHLVINYLDTKTSIIHKHDILLPGEHNLENVLAATTATLPFSISEENIRNTLKAFSGVD
HRLELVRELNGVKFINDSKGTNVEASLKAINSFDSMILIAGGKDKGADFSEFAEAIKEKGVKKVYLFGETYKKIASALDK
VYYPEYYIVNNLETAVTGAYQDAISGDVVLLSPACASWDMYDSFEKRGNHFKSIVYSLGGE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=469, Percent_Identity=32.1961620469083, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786274, Length=306, Percent_Identity=24.8366013071895, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786279, Length=297, Percent_Identity=22.8956228956229, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_NATTJ (B2A2H0)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917473.1
- ProteinModelPortal:   B2A2H0
- SMR:   B2A2H0
- GeneID:   6315165
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1302
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 51289; Mature: 51289

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDDKGKYLILGLGKSGQGAVIGLSNFEDCQVAVTDRKNLDDIRDAAAILEEHNVPYLNE
CCCCCCCEEEEEECCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCC
RESVERLSEFDILVKSPGIPMDNNIITKAKELGMPVIDELELGYQILNYRLTKPEERLIA
HHHHHHHHHCCEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCCEEEE
VTGTNGKTTTTNLIGEFFEASGYSIYVAGNVGLPLSQIACEVTDTDFIVLEVSSFQLEFI
EECCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHEEEECCCCEEEEEECCHHHHHH
KGFRPKVSMILNITPDHLDWHGDLDSYIRAKMNIFKNQTSKDFSVVNVDDETTFGLNRES
HCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCC
KGRKLNFSKNSLRTEGSFIEDDHLVINYLDTKTSIIHKHDILLPGEHNLENVLAATTATL
CCCEECCCHHCCCCCCCEECCCCEEEEEECCCHHEEEECCEEECCCCCHHHHHHHHHEEC
PFSISEENIRNTLKAFSGVDHRLELVRELNGVKFINDSKGTNVEASLKAINSFDSMILIA
CEECCHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEEEE
GGKDKGADFSEFAEAIKEKGVKKVYLFGETYKKIASALDKVYYPEYYIVNNLETAVTGAY
CCCCCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHH
QDAISGDVVLLSPACASWDMYDSFEKRGNHFKSIVYSLGGE
HHHCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure
MFDDKGKYLILGLGKSGQGAVIGLSNFEDCQVAVTDRKNLDDIRDAAAILEEHNVPYLNE
CCCCCCCEEEEEECCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCC
RESVERLSEFDILVKSPGIPMDNNIITKAKELGMPVIDELELGYQILNYRLTKPEERLIA
HHHHHHHHHCCEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCCEEEE
VTGTNGKTTTTNLIGEFFEASGYSIYVAGNVGLPLSQIACEVTDTDFIVLEVSSFQLEFI
EECCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHEEEECCCCEEEEEECCHHHHHH
KGFRPKVSMILNITPDHLDWHGDLDSYIRAKMNIFKNQTSKDFSVVNVDDETTFGLNRES
HCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCC
KGRKLNFSKNSLRTEGSFIEDDHLVINYLDTKTSIIHKHDILLPGEHNLENVLAATTATL
CCCEECCCHHCCCCCCCEECCCCEEEEEECCCHHEEEECCEEECCCCCHHHHHHHHHEEC
PFSISEENIRNTLKAFSGVDHRLELVRELNGVKFINDSKGTNVEASLKAINSFDSMILIA
CEECCHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEEEE
GGKDKGADFSEFAEAIKEKGVKKVYLFGETYKKIASALDKVYYPEYYIVNNLETAVTGAY
CCCCCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHH
QDAISGDVVLLSPACASWDMYDSFEKRGNHFKSIVYSLGGE
HHHCCCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA