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The map label for this gene is nuoE [H]

Identifier: 188585873

GI number: 188585873

Start: 1316345

End: 1316821

Strand: Direct

Name: nuoE [H]

Synonym: Nther_1247

Alternate gene names: 188585873

Gene position: 1316345-1316821 (Clockwise)

Preceding gene: 188585872

Following gene: 188585874

Centisome position: 41.58

GC content: 39.83

Gene sequence:

>477_bases
GTGTCTCAAGAAAAAACCATTGGAACAGAGGAAGTCAAAAAAGTAGAGAATCTAGTCAAAAAACACCAAGATAAAAAAGG
TGCAGCTATTCCAATTTTGCAAGACATACAAAAGGAGTTGGGGTACATTCCTAAAGAGGTGTTACCAAAAGTTACCTCTT
TAACAAAGATTCCAGAAAGTGACCTCTACAGTATTGTTACATTTTATTCTCAATTTAGATTGCAGCCCGTTGGTGATAAT
TTGATTCATGTTTGTCACGGAACTGCATGTCACTTGGCTGGTGCCGAGGAGATCACCAATGCTTTAGTACGAGAATTAGA
GATTGGCGATGAAGGTACAAGCCCCGATGGTAAATTTACTGTCGAAAAAGTAGCCTGCCTCGGTTGCTGCAGTTTAGCAC
CAGTTATGACTATAAATGGTGAAACTCACGGCAGGCTGACTCCTGACAAAGCTGTTAAAATAGCTAAAAACATCTAG

Upstream 100 bases:

>100_bases
AATATCTAGGACAGGCTTAGTTTTTAAGTCATTAGTAATAAAGATTAATTAATGTATTAAGTATTTAGGGAGGACATATT
TTTCTGTTAGGAGGGATAAC

Downstream 100 bases:

>100_bases
GAGGTGGACAAAAGTATGGTACCAGTTGTAAAAATCGGAATGGCCAGTTGTGGTATAGCTGCAGGGGCTAAAAAAGTTTA
TGACCTTTTCTCCGATGAAG

Product: NADH dehydrogenase (ubiquinone) 24 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSQEKTIGTEEVKKVENLVKKHQDKKGAAIPILQDIQKELGYIPKEVLPKVTSLTKIPESDLYSIVTFYSQFRLQPVGDN
LIHVCHGTACHLAGAEEITNALVRELEIGDEGTSPDGKFTVEKVACLGCCSLAPVMTINGETHGRLTPDKAVKIAKNI

Sequences:

>Translated_158_residues
MSQEKTIGTEEVKKVENLVKKHQDKKGAAIPILQDIQKELGYIPKEVLPKVTSLTKIPESDLYSIVTFYSQFRLQPVGDN
LIHVCHGTACHLAGAEEITNALVRELEIGDEGTSPDGKFTVEKVACLGCCSLAPVMTINGETHGRLTPDKAVKIAKNI
>Mature_157_residues
SQEKTIGTEEVKKVENLVKKHQDKKGAAIPILQDIQKELGYIPKEVLPKVTSLTKIPESDLYSIVTFYSQFRLQPVGDNL
IHVCHGTACHLAGAEEITNALVRELEIGDEGTSPDGKFTVEKVACLGCCSLAPVMTINGETHGRLTPDKAVKIAKNI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=140, Percent_Identity=34.2857142857143, Blast_Score=93, Evalue=1e-19,
Organism=Escherichia coli, GI1788621, Length=143, Percent_Identity=34.965034965035, Blast_Score=104, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17561328, Length=134, Percent_Identity=37.3134328358209, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI18859877, Length=140, Percent_Identity=35.7142857142857, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21355353, Length=133, Percent_Identity=29.3233082706767, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17224; Mature: 17093

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEKTIGTEEVKKVENLVKKHQDKKGAAIPILQDIQKELGYIPKEVLPKVTSLTKIPES
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
DLYSIVTFYSQFRLQPVGDNLIHVCHGTACHLAGAEEITNALVRELEIGDEGTSPDGKFT
HHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCH
VEKVACLGCCSLAPVMTINGETHGRLTPDKAVKIAKNI
HHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SQEKTIGTEEVKKVENLVKKHQDKKGAAIPILQDIQKELGYIPKEVLPKVTSLTKIPES
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
DLYSIVTFYSQFRLQPVGDNLIHVCHGTACHLAGAEEITNALVRELEIGDEGTSPDGKFT
HHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCH
VEKVACLGCCSLAPVMTINGETHGRLTPDKAVKIAKNI
HHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]