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The map label for this gene is dnaG [H]

Identifier: 188585840

GI number: 188585840

Start: 1286489

End: 1288369

Strand: Direct

Name: dnaG [H]

Synonym: Nther_1214

Alternate gene names: 188585840

Gene position: 1286489-1288369 (Clockwise)

Preceding gene: 188585839

Following gene: 188585841

Centisome position: 40.64

GC content: 35.46

Gene sequence:

>1881_bases
ATGACAATCCGAATACCCCAGGAAATTATAGAACGAATTCAAGCTGAAGCCGACTTAGTAGAAATTGCATCAAGGTATGT
GAACTTAAAAAAACAAGGTAAGTATTACTGGGGTCTATGTCCCTTTCATAGCGAAAAAACTCCGTCATTTCATATTTCAC
CTGAAAAGCAATTGTACTATTGTTTTGGTTGTCAAGCAGGAGGGAATGTTTTTAAATTTCTCGAATCTATTGAACAACTA
AACTTTGTTGAGGTTTGTCAAAAGTTGGGTGAAGAACTAGGAATTGAGTTGCCAGCGGATTCCCAAGACAGTCAAGAGTT
ATCCCAGGAAATGCAGGAACGAGAAATTATTAAAAAAGCCAATAAATATGCAGCAAACTTTTTTAGATACGTACTAATGA
ATACCGAAACTGGGAAATCTGGGAGAAAATACTTGGCTGATAGAAAAATTGACAAGCAGACGATGGCGGACTTTTTTATT
GGATACGCTCCCGATAACTGGACTGGTCTGAGTGATTTCTTAAAGAAAAAAGGTTTCACTGAGGAATTACTAGTTAAAGC
TGGTCTAAGTAATCGTAGGAAATATCGTGAGGGGGTATACGACAGATTTAGAGGAAGGGTAATTTTTCCTATTTATAACC
AAAGAGGCGAAGTGATCGGTTTCGGTGGAAGGCTATTAGAGGAGAGTGAAAAGCAACCTAAGTATTTAAATTCTCCTGAA
ACTCCTGTTTTTAATAAAAGATTAAGTTTATATGGTTTAAACTTAGCTTTTAAAACAATTCGAAAAGAAAATCATGCCTT
GGTGATGGAAGGTTATACTGATGTGATCACTGCTCATCAATTTGGTGTGAATACGGCAGTAGCGTCTTTAGGAACTTCTT
TAACTGAAGAACAAGCTAAACTAATTAGAAATAATGCTAGCAAGGCTTATATAGCCTTTGACGCAGATGCTGCAGGAGAG
ACTGCCACTCTCCGTGGATTAGAAATTTTGCAAAGTAAAGGCTTGGATGTCTTGATTTGTCAGCTTCCAGAGTCTTATGA
CCCTGATGAGTATTTAAAAAACTATGGACTAGAAAATTTTCAAAAGAAAATTTTAGACACGGCTTTGCCACTAACTGAAT
ATCAAATAAGACAAGCTGCTAAAAACAAAAATTTGTCAACAATAGAAGGAAAAAAGCAATACGTAACAGAAATAATCCCT
ATAATTTCAAAAATCGATAATGCAGTGGAGCGCGAGGAATATCTGAATCAAGTATCAGAGCAGTTAGGAATTTCTCAAGA
AGCACTCAATTCTGAACTCATGAAATATTTTAGAAAAAATTACAGAAATAAAAATTATCGCAAAATGGATAAAACTGGAG
TAAACAGGAAGAATAAAAGAAAAAATAATGGAAGTGAAACTGTGAAATTTGCTGACAAAAGGACACCACGCGAAAAAGCA
GAAGAAGGATTGGTGGCTCTAATGTTGCATTTTCCTGAATATACTGAAGAAGTGAATAAATTTTTGTACCCTGAAGATTT
TTCTAGTGATACTTTAGCTCTTATTGTTCATAAACTGTATCAGTTGAAGAATCACGACATTGAACTAGATCTGTCGAATC
TCACCCAGGAATTAAGAGATCACCCAGATGCAGAAAATATCATTACTAAACTTGGTATGAAGGATTTTCCGCAGCCAAAG
GATATAGAAAAGTTTGTAAAGGACTGTGTATTTAGAATTAAGGAAAGCAGAATTAAGAGTCAAAGAGAAGAGCTACAAAA
GGAGCTTTCTCAAATAAATCCTGATCATGAACCAGATAGATATAGAGAAATTCTGAGAAAGTTGCAAGATTGCTTGTATA
TAGAAAAAACAAAACAGTGGACCTTGGAGGAAGGGGGGTAA

Upstream 100 bases:

>100_bases
ATTTTATTTTTTAGCAGGATTTTTTTTAATGTTAGAGAAATGAATATACTTGTCGTTTCATGATGATTTATGTAGAAGGT
TATCTGAGGGGGATATCCAT

Downstream 100 bases:

>100_bases
AATTATGGCAGAGAAAAATCAAAACCAAAAATTAGAACAAATTAAAGATGAACTGTTAAAAGTGGGAAAAGAACGAGGAT
TTCTCACCTATAAAGAAATT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MTIRIPQEIIERIQAEADLVEIASRYVNLKKQGKYYWGLCPFHSEKTPSFHISPEKQLYYCFGCQAGGNVFKFLESIEQL
NFVEVCQKLGEELGIELPADSQDSQELSQEMQEREIIKKANKYAANFFRYVLMNTETGKSGRKYLADRKIDKQTMADFFI
GYAPDNWTGLSDFLKKKGFTEELLVKAGLSNRRKYREGVYDRFRGRVIFPIYNQRGEVIGFGGRLLEESEKQPKYLNSPE
TPVFNKRLSLYGLNLAFKTIRKENHALVMEGYTDVITAHQFGVNTAVASLGTSLTEEQAKLIRNNASKAYIAFDADAAGE
TATLRGLEILQSKGLDVLICQLPESYDPDEYLKNYGLENFQKKILDTALPLTEYQIRQAAKNKNLSTIEGKKQYVTEIIP
IISKIDNAVEREEYLNQVSEQLGISQEALNSELMKYFRKNYRNKNYRKMDKTGVNRKNKRKNNGSETVKFADKRTPREKA
EEGLVALMLHFPEYTEEVNKFLYPEDFSSDTLALIVHKLYQLKNHDIELDLSNLTQELRDHPDAENIITKLGMKDFPQPK
DIEKFVKDCVFRIKESRIKSQREELQKELSQINPDHEPDRYREILRKLQDCLYIEKTKQWTLEEGG

Sequences:

>Translated_626_residues
MTIRIPQEIIERIQAEADLVEIASRYVNLKKQGKYYWGLCPFHSEKTPSFHISPEKQLYYCFGCQAGGNVFKFLESIEQL
NFVEVCQKLGEELGIELPADSQDSQELSQEMQEREIIKKANKYAANFFRYVLMNTETGKSGRKYLADRKIDKQTMADFFI
GYAPDNWTGLSDFLKKKGFTEELLVKAGLSNRRKYREGVYDRFRGRVIFPIYNQRGEVIGFGGRLLEESEKQPKYLNSPE
TPVFNKRLSLYGLNLAFKTIRKENHALVMEGYTDVITAHQFGVNTAVASLGTSLTEEQAKLIRNNASKAYIAFDADAAGE
TATLRGLEILQSKGLDVLICQLPESYDPDEYLKNYGLENFQKKILDTALPLTEYQIRQAAKNKNLSTIEGKKQYVTEIIP
IISKIDNAVEREEYLNQVSEQLGISQEALNSELMKYFRKNYRNKNYRKMDKTGVNRKNKRKNNGSETVKFADKRTPREKA
EEGLVALMLHFPEYTEEVNKFLYPEDFSSDTLALIVHKLYQLKNHDIELDLSNLTQELRDHPDAENIITKLGMKDFPQPK
DIEKFVKDCVFRIKESRIKSQREELQKELSQINPDHEPDRYREILRKLQDCLYIEKTKQWTLEEGG
>Mature_625_residues
TIRIPQEIIERIQAEADLVEIASRYVNLKKQGKYYWGLCPFHSEKTPSFHISPEKQLYYCFGCQAGGNVFKFLESIEQLN
FVEVCQKLGEELGIELPADSQDSQELSQEMQEREIIKKANKYAANFFRYVLMNTETGKSGRKYLADRKIDKQTMADFFIG
YAPDNWTGLSDFLKKKGFTEELLVKAGLSNRRKYREGVYDRFRGRVIFPIYNQRGEVIGFGGRLLEESEKQPKYLNSPET
PVFNKRLSLYGLNLAFKTIRKENHALVMEGYTDVITAHQFGVNTAVASLGTSLTEEQAKLIRNNASKAYIAFDADAAGET
ATLRGLEILQSKGLDVLICQLPESYDPDEYLKNYGLENFQKKILDTALPLTEYQIRQAAKNKNLSTIEGKKQYVTEIIPI
ISKIDNAVEREEYLNQVSEQLGISQEALNSELMKYFRKNYRNKNYRKMDKTGVNRKNKRKNNGSETVKFADKRTPREKAE
EGLVALMLHFPEYTEEVNKFLYPEDFSSDTLALIVHKLYQLKNHDIELDLSNLTQELRDHPDAENIITKLGMKDFPQPKD
IEKFVKDCVFRIKESRIKSQREELQKELSQINPDHEPDRYREILRKLQDCLYIEKTKQWTLEEGG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=443, Percent_Identity=37.2460496613996, Blast_Score=268, Evalue=9e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 72539; Mature: 72408

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRIPQEIIERIQAEADLVEIASRYVNLKKQGKYYWGLCPFHSEKTPSFHISPEKQLYY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCCCEEE
CFGCQAGGNVFKFLESIEQLNFVEVCQKLGEELGIELPADSQDSQELSQEMQEREIIKKA
EECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
NKYAANFFRYVLMNTETGKSGRKYLADRKIDKQTMADFFIGYAPDNWTGLSDFLKKKGFT
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCH
EELLVKAGLSNRRKYREGVYDRFRGRVIFPIYNQRGEVIGFGGRLLEESEKQPKYLNSPE
HHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCHHHHHHCCCCCCCCCCC
TPVFNKRLSLYGLNLAFKTIRKENHALVMEGYTDVITAHQFGVNTAVASLGTSLTEEQAK
CCHHHCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LIRNNASKAYIAFDADAAGETATLRGLEILQSKGLDVLICQLPESYDPDEYLKNYGLENF
HHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCHHHH
QKKILDTALPLTEYQIRQAAKNKNLSTIEGKKQYVTEIIPIISKIDNAVEREEYLNQVSE
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLGISQEALNSELMKYFRKNYRNKNYRKMDKTGVNRKNKRKNNGSETVKFADKRTPREKA
HHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCHHEEEHHCCCCHHHH
EEGLVALMLHFPEYTEEVNKFLYPEDFSSDTLALIVHKLYQLKNHDIELDLSNLTQELRD
HCCHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHC
HPDAENIITKLGMKDFPQPKDIEKFVKDCVFRIKESRIKSQREELQKELSQINPDHEPDR
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
YREILRKLQDCLYIEKTKQWTLEEGG
HHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TIRIPQEIIERIQAEADLVEIASRYVNLKKQGKYYWGLCPFHSEKTPSFHISPEKQLYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCCCEEE
CFGCQAGGNVFKFLESIEQLNFVEVCQKLGEELGIELPADSQDSQELSQEMQEREIIKKA
EECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
NKYAANFFRYVLMNTETGKSGRKYLADRKIDKQTMADFFIGYAPDNWTGLSDFLKKKGFT
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCH
EELLVKAGLSNRRKYREGVYDRFRGRVIFPIYNQRGEVIGFGGRLLEESEKQPKYLNSPE
HHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCHHHHHHCCCCCCCCCCC
TPVFNKRLSLYGLNLAFKTIRKENHALVMEGYTDVITAHQFGVNTAVASLGTSLTEEQAK
CCHHHCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LIRNNASKAYIAFDADAAGETATLRGLEILQSKGLDVLICQLPESYDPDEYLKNYGLENF
HHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCHHHH
QKKILDTALPLTEYQIRQAAKNKNLSTIEGKKQYVTEIIPIISKIDNAVEREEYLNQVSE
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLGISQEALNSELMKYFRKNYRNKNYRKMDKTGVNRKNKRKNNGSETVKFADKRTPREKA
HHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCHHEEEHHCCCCHHHH
EEGLVALMLHFPEYTEEVNKFLYPEDFSSDTLALIVHKLYQLKNHDIELDLSNLTQELRD
HCCHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHC
HPDAENIITKLGMKDFPQPKDIEKFVKDCVFRIKESRIKSQREELQKELSQINPDHEPDR
CCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
YREILRKLQDCLYIEKTKQWTLEEGG
HHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]