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The map label for this gene is sigA [H]

Identifier: 188585841

GI number: 188585841

Start: 1288374

End: 1289456

Strand: Direct

Name: sigA [H]

Synonym: Nther_1215

Alternate gene names: 188585841

Gene position: 1288374-1289456 (Clockwise)

Preceding gene: 188585840

Following gene: 188585842

Centisome position: 40.7

GC content: 39.61

Gene sequence:

>1083_bases
ATGGCAGAGAAAAATCAAAACCAAAAATTAGAACAAATTAAAGATGAACTGTTAAAAGTGGGAAAAGAACGAGGATTTCT
CACCTATAAAGAAATTATGGATCAGTTGTCAGAATTCCAGCTTTCCAGCGACCAAATAGATGAATTTTATGAAGTACTGA
CTCAGGCCGGTATAGATGTGGTGGATGCTATTGGAGAGGTGGATCCAGATGACCCCACAAAAGCGGAGCTGGACTTATCA
CTTCCTGAAGGGGTAGAAGTAAGTGATCCGGTACGTATGTACTTAAAAGAGATCGGAAAAATCCCCCTTCTATCTCCCGA
CGACGAAATAGAACTTGCCAAGAAAATTGAAGAGGGAGATGAAGGAGCTAAAAGAAAATTGGCTGAAGCCAATTTACGCT
TAGTGGTCAGTATTGCTAAAAAATACGTTGGTCGCGGGATGTTATTTCTGGACTTGATTCAAGAAGGTAATTTGGGACTG
ATAAAAGCAGTAGAAAAATTTGATTACCGAAAAGGTTACAAGTTTAGTACTTATGCTACATGGTGGATACGTCAAGCAAT
TACAAGGGCCATAGCAGATCAAGCTAGGACTATTAGAATACCTGTCCATATGGTGGAAACTATCAATAAATTGATTAGAG
TTTCGCGTCAACTCGTACAGGAACTGGGTAGGGAACCTTTACCTGAAGAAATTGCAGAACAAATGGATATGAGTGAAGAC
AAGGTCAGGGAAATCTTAAAAATTGCCCAGGAACCCGTAAGTTTAGAGACTCCTATAGGAGAAGAGGATGACAGTCACTT
AGGAGACTTTATAGAAGACCAGGATGCTAAACCACCTGCAGATGCTGCTGCTTTTGAACTTCTTAAAGGACAATTAGAAG
AAGTATTGGATAGTTTAACACCCCGTGAGCAAAAAGTATTGAGACTTAGATTTGGATTAGATGACGGAAAAACTCGAACT
CTAGAAGAGGTGGGTAAACACTTTGGTGTCACTAGGGAAAGGATTAGACAAATAGAAGCTAAAGCCTTGCGTAAATTACG
TCACCCTATGAGAAGTAAACGCCTTAAGGATTACTTAGATTAA

Upstream 100 bases:

>100_bases
CATGAACCAGATAGATATAGAGAAATTCTGAGAAAGTTGCAAGATTGCTTGTATATAGAAAAAACAAAACAGTGGACCTT
GGAGGAAGGGGGGTAAAATT

Downstream 100 bases:

>100_bases
ATAAAATTTAAATTTACTCTTTACAAAAGACGTTTTTTTTGGTATAAATTATTGTGGGCGGCGGAAATGGCTCGACGACA
GCGACAGATTTTCAAACAAA

Product: RNA polymerase, sigma 70 subunit, RpoD family

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MAEKNQNQKLEQIKDELLKVGKERGFLTYKEIMDQLSEFQLSSDQIDEFYEVLTQAGIDVVDAIGEVDPDDPTKAELDLS
LPEGVEVSDPVRMYLKEIGKIPLLSPDDEIELAKKIEEGDEGAKRKLAEANLRLVVSIAKKYVGRGMLFLDLIQEGNLGL
IKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLVQELGREPLPEEIAEQMDMSED
KVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAKPPADAAAFELLKGQLEEVLDSLTPREQKVLRLRFGLDDGKTRT
LEEVGKHFGVTRERIRQIEAKALRKLRHPMRSKRLKDYLD

Sequences:

>Translated_360_residues
MAEKNQNQKLEQIKDELLKVGKERGFLTYKEIMDQLSEFQLSSDQIDEFYEVLTQAGIDVVDAIGEVDPDDPTKAELDLS
LPEGVEVSDPVRMYLKEIGKIPLLSPDDEIELAKKIEEGDEGAKRKLAEANLRLVVSIAKKYVGRGMLFLDLIQEGNLGL
IKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLVQELGREPLPEEIAEQMDMSED
KVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAKPPADAAAFELLKGQLEEVLDSLTPREQKVLRLRFGLDDGKTRT
LEEVGKHFGVTRERIRQIEAKALRKLRHPMRSKRLKDYLD
>Mature_359_residues
AEKNQNQKLEQIKDELLKVGKERGFLTYKEIMDQLSEFQLSSDQIDEFYEVLTQAGIDVVDAIGEVDPDDPTKAELDLSL
PEGVEVSDPVRMYLKEIGKIPLLSPDDEIELAKKIEEGDEGAKRKLAEANLRLVVSIAKKYVGRGMLFLDLIQEGNLGLI
KAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLVQELGREPLPEEIAEQMDMSEDK
VREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAKPPADAAAFELLKGQLEEVLDSLTPREQKVLRLRFGLDDGKTRTL
EEVGKHFGVTRERIRQIEAKALRKLRHPMRSKRLKDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=70.5882352941177, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1789098, Length=306, Percent_Identity=43.4640522875817, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=30.1470588235294, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=29.2079207920792, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41167; Mature: 41036

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKNQNQKLEQIKDELLKVGKERGFLTYKEIMDQLSEFQLSSDQIDEFYEVLTQAGIDV
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHH
VDAIGEVDPDDPTKAELDLSLPEGVEVSDPVRMYLKEIGKIPLLSPDDEIELAKKIEEGD
HHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
EGAKRKLAEANLRLVVSIAKKYVGRGMLFLDLIQEGNLGLIKAVEKFDYRKGYKFSTYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
WWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLVQELGREPLPEEIAEQMDMSED
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHH
KVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAKPPADAAAFELLKGQLEEVLDSLT
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
PREQKVLRLRFGLDDGKTRTLEEVGKHFGVTRERIRQIEAKALRKLRHPMRSKRLKDYLD
CHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEKNQNQKLEQIKDELLKVGKERGFLTYKEIMDQLSEFQLSSDQIDEFYEVLTQAGIDV
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHH
VDAIGEVDPDDPTKAELDLSLPEGVEVSDPVRMYLKEIGKIPLLSPDDEIELAKKIEEGD
HHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
EGAKRKLAEANLRLVVSIAKKYVGRGMLFLDLIQEGNLGLIKAVEKFDYRKGYKFSTYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
WWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLVQELGREPLPEEIAEQMDMSED
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHH
KVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAKPPADAAAFELLKGQLEEVLDSLT
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
PREQKVLRLRFGLDDGKTRTLEEVGKHFGVTRERIRQIEAKALRKLRHPMRSKRLKDYLD
CHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987246; 3086839; 8969508; 9384377; 2166045; 8363585 [H]